Article
Food Science & Technology
Hongji Ye, Xinyi Zhang, Yang Jiang, Min Guo, Xiaoming Liu, Jianxin Zhao, Hao Zhang, Wei Chen
Summary: This study investigated the peptidomic patterns of fermented milk by different strains of Lactobacillus delbrueckii ssp. bulgaricus and ssp. lactis before and after simulated gastrointestinal digestion. Variations in peptidomics were found among samples, with different subspecies displaying distinct peptide profiles. Digestion appeared to reduce peptidomics variations and the strain L. delbrueckii ssp. bulgaricus DQHXNS8L6 was the most efficient in generating bioactive peptides.
Article
Genetics & Heredity
Estrada Bonilla, Ana Rita Costa, Daan F. van den Berg, Teunke van Rossum, Stefan Hagedoorn, Hielke Walinga, Minfeng Xiao, Wenchen Song, Pieter-Jan Haas, Franklin L. Nobrega, Stan J. J. Brouns
Summary: This study reveals new genetic diversity from bacteriophages and identifies novel proteins and genes. Three of the bacteriophages encode tRNAs and have a GC content dissimilar to the host, suggesting potential biotechnological and medical applications. The study contributes to understanding previously unknown virus diversity and provides new candidates for phage therapy against antibiotic-resistant K. pneumoniae infections.
Article
Virology
Sabrina Sprotte, Olakunle Fagbemigun, Erik Brinks, Gyu-Sung Cho, Eoghan Casey, Folarin A. Oguntoyinbo, Horst Neve, Jennifer Mahony, Douwe van Sinderen, Charles M. A. P. Franz
Summary: A new Lactobacillus delbrueckii bacteriophage PMBT4 was isolated from nono and belongs to the Cequinquevirus genus. It possesses a collar-like neck passage structure and high sequence homologies to other Lb. delbrueckii phages.
Article
Entomology
Inga Bazukyan, Dimitrina Georgieva-Miteva, Tsvetelina Velikova, Svetoslav G. Dimov
Summary: This study reports the isolation of L. delbrueckii strain from the gut of a honeybee for the first time. Genomic analysis revealed that the strain possesses probiotic properties and has adapted to endosymbiotic conditions. This potentially endosymbiotic strain is capable of playing beneficial roles for the honeybee host.
Article
Biochemistry & Molecular Biology
Mariano Elean, Leonardo Albarracin, Julio Villena, Haruki Kitazawa, Lucila Saavedra, Elvira M. Hebert
Summary: A comprehensive genomic analysis of L. delbrueckii strains was conducted, focusing on the proteolytic system and the key enzyme, cell envelope-associated proteinase (CEP). The study compared amino acid sequences and protein structures of CEPs, as well as analyzed syntenic and genomic variations among different strains. Investigation of CEP promoter region and regulatory elements provided insights into the control of proteolytic activity in lactobacilli. This study contributes to our understanding of proteolytic activity and potential applications of L. delbrueckii in the dairy industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Elena Bancalari, Monica Gatti, Benedetta Bottari, Diego Mora, Stefania Arioli
Summary: The production of exopolysaccharides by 3 strains of Lactobacillus delbrueckii subsp. bulgaricus was evaluated using impedometric measurements, fluorescent microscopy, and flow cytometry analyses. The combined approach revealed differences in EPS production kinetics and highlighted the importance of EPS-producing cells within the same population. This study provides insights for selecting strains for dairy products where EPS production is a key feature.
Article
Biochemistry & Molecular Biology
Mohamad Ismail Sultan-Alolama, Amr Amin, Ranjit Vijayan, Khaled A. A. El-Tarabily
Summary: In this study, a virulent bacteriophage named Ec_MI-02 was isolated from the feces of a wild pigeon in the UAE, which can be used as a bio-preservative or in phage therapy. The phage was found to infect multiple serotypes of E. coli O157:H7. The research provides evidence that wild birds may serve as natural reservoirs for phages without antibiotic resistance genes, and studying the genetic makeup of phages that infect human pathogens is crucial for their safe usage in the food industry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Ireneusz Sobolewski, Katarzyna Adamowicz, Anna Struck, Agnieszka Zylicz-Stachula, Piotr M. Skowron
Summary: DNA purification methods are important tools in molecular biology, but currently available techniques may not be effective for certain specialized applications. We have developed a method that uses silica columns and mini-scale isolation to efficiently purify bacteriophage genomic DNA from concentrated phage preparations, without the need for removing CsCl solutions.
Article
Chemistry, Applied
Ao Liu, Qiqi Liu, Yushan Bu, Haining Hao, Tongjie Liu, Pimin Gong, Lanwei Zhang, Chen Chen, Huaixiang Tian, Huaxi Yi
Summary: The aroma of fermented milk produced by 28 strains of Lactobacillus delbrueckii subsp. bulgaricus was evaluated and classified into different types. A total of 12 key aroma compounds were identified, which can be used to develop fermented milk products with specific aroma profiles.
Article
Microbiology
Nikita Zrelovs, Andris Dislers, Andris Kazaks
Summary: Researchers isolated a unique phage, Nocturne116, from a deceased local moth specimen, revealing a genome length of 25,554 bp with 52 predicted open reading frames. Despite the availability of numerous complete genomes of Lactococcus phages, Nocturne116 stands out as a novel genus due to its distinct genetic characteristics.
Article
Biochemistry & Molecular Biology
Shuai Zhang, Dongyao Li, Xin Zhang, Yongsheng Sun, Sha Xu, Xinyu Wang, Na Zhang, Miaoshu Wang, Hongtao Tian, Chen Li
Summary: The study identified genes that persistently responded to acid during yogurt fermentation and were less affected by cold stress, suggesting their potential involvement in post-acidification. Among these genes, LDB_RS05285 was found to be crucial in inhibiting post-acidification and could be a new target for regulating yogurt quality.
Article
Virology
Chunyang Zhu, Jing Li, Yang Liu, Fenhua Jin, Zhanxin Wang, Yigang Tong, Jianping Qin, Binghu Fang
Summary: In this study, a new Salmonella phage, NX263, was isolated from sewage. The phage showed high lytic activity against a wide range of bacterial strains tested, as well as good stability at different temperatures and pH levels. Genomic analysis revealed its potential as a therapeutic agent for Salmonella Pullorum infections.
ARCHIVES OF VIROLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Gwenael Jan, Florian Tarnaud, Fillipe Luiz Rosa do Carmo, Nassima Illikoud, Fanny Canon, Julien Jardin, Valerie Briard-Bion, Fanny Guyomarc'h, Valerie Gagnaire
Summary: Lactobacillus delbrueckii subsp. bulgaricus, a commonly used yogurt starter, shows poor adaptation to soy milk. It does not grow in soy milk, but can accelerate its acidification when co-cultured with Streptococcus thermophilus. Comparison with cow's milk reveals significant differences in cell morphology and proteome composition in soy milk, indicating stress response and downregulation of cell division machinery.
Article
Microbiology
Khashayar Shahin, Mohadeseh Barazandeh, Lili Zhang, Abolghasem Hedayatkhah, Tao He, Hongduo Bao, Mojtaba Mansoorianfar, Maoda Pang, Heye Wang, Ruicheng Wei, Ran Wang
Summary: This study isolated three lytic phages from an aquatic ecosystem, indicating the importance of continued isolation on non-model members of Enterobacteriaceae family to fully understand bacteriophage diversity in aquatic environments. The phages showed high temperature and pH stability and did not harbor any genes involved in lysogenic cycles.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Y. Suda, K. Kagawa, K. Fukuyama, M. Elean, B. Zhou, M. Tomokiyo, M. Aminul Islam, M. S. R. Rajoka, A. K. M. Humayun Kober, T. Shimazu, S. Egusa, Y. Terashima, H. Aso, W. Ikeda-Ohtsubo, J. Villena, H. Kitazawa
Summary: This study found that soymilk fermented with L. delbrueckii subsp. delbrueckii TUA4408L reduced inflammation and regulated cytokine expression in the intestinal mucosa of pigs. These immunological changes were associated with improved resistance to pathogenic Escherichia coli and enhanced growth performance and meat quality.
BENEFICIAL MICROBES
(2022)
Review
Food Science & Technology
Valentina Ambrogi, Francesca Bottacini, Linqiu Cao, Bas Kuipers, Margriet Schoterman, Douwe van Sinderen
Summary: Galacto-oligosaccharides (GOS) are non-digestible oligosaccharides with diverse structures that have been extensively studied for their health-promoting effects. GOS have been reported to promote the growth of beneficial bacteria, inhibit pathogen adhesion, and improve gut barrier function. However, their industrial production still faces technological challenges.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Katherine Lavelle, Irina Sadovskaya, Evgeny Vinogradov, Philip Kelleher, Gabriele A. Lugli, Marco Ventura, Douwe van Sinderen, Jennifer Mahony
Summary: This study reveals that the rgp gene cluster in Streptococcus thermophilus encodes the biosynthetic machinery for a polysaccharide essential for phage adsorption to the host cells.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Biochemical Research Methods
Giulia Alessandri, Sonia M. Rizzo, Maria C. Ossiprandi, Douwe van Sinderen, Marco Ventura
Summary: Given the importance of the intestinal microbial community in host health, there have been increased efforts to determine its composition and functions. Next-Generation Sequencing techniques and bioinformatic tools have provided an exciting opportunity to expand our knowledge on intestinal microbial communities. Factors such as diet, host phylogeny, host physiology, and human activities play a major role in selecting a specific gut microbial consortium in mammals.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Review
Biochemical Research Methods
Jennifer Mahony, Douwe van Sinderen
Summary: The food industry is facing pressure to produce high quality, traceable and minimally processed foods using sustainable approaches and ingredients. This includes a shift towards plant-based products and the need for research on microbial requirements, composition, and the role of phages in shaping the microbial landscape of these foods.
CURRENT OPINION IN BIOTECHNOLOGY
(2022)
Article
Microbiology
Chiara Argentini, Federico Fontana, Giulia Alessandri, Gabriele Andrea Lugli, Leonardo Mancabelli, Maria Cristina Ossiprandi, Douwe van Sinderen, Marco Ventura, Christian Milani, Francesca Turroni
Summary: This study investigates the colonization mechanism and antimicrobial activity of Lactobacillus crispatus in the vaginal ecological niche. The results reveal the genetic variability of this species and its adaptation to the niche to enhance competitiveness for colonization.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Dacil Rivera, Andrea Moreno-Switt, Thomas G. Denes, Lauren K. Hudson, Tracey L. Peters, Reham Samir, Ramy K. Aziz, Jean-Paul Noben, Jeroen Wagemans, Fernando Duenas
Summary: Salmonellosis is a common zoonotic foodborne disease, and poultry is a major reservoir for the bacteria. This study introduces a newly isolated bacteriophage STGO-35-1, which effectively reduces Salmonella enterica serovar Enteritidis in chicken meat. The phage's genomic and phenotypic characteristics, as well as its potential as a biocontrol agent, are evaluated.
Article
Biochemistry & Molecular Biology
Jorge Lopez-Tello, Zoe Schofield, Raymond Kiu, Matthew J. Dalby, Douwe van Sinderen, Gwenaelle Le Gall, Amanda N. Sferruzzi-Perri, Lindsay J. Hall
Summary: This study demonstrates that the bacterium Bifidobacterium breve UCC2003, a key member of the maternal gut microbiota, modulates maternal body adaptations, placental structure, and nutrient transporter capacity, with implications for fetal metabolism and growth.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Ecology
Miriam N. Ojima, Lin Jiang, Aleksandr A. Arzamasov, Keisuke Yoshida, Toshitaka Odamaki, Jinzhong Xiao, Aruto Nakajima, Motomitsu Kitaoka, Junko Hirose, Tadasu Urashima, Toshihiko Katoh, Aina Gotoh, Douwe van Sinderen, Dmitry A. Rodionov, Andrei L. Osterman, Mikiyasu Sakanaka, Takane Katayama
Summary: This study applied assembly theory to investigate the formation of bifidobacterial communities in the infant gut. The results showed that arrival order and sugar consumption phenotypes significantly affected community formation. Bifidobacterium bifidum and Bifidobacterium longum subsp. infantis dominated through inhibitory priority effects, while Bifidobacterium breve benefited from facilitative priority effects and utilized a specific degradant to dominate. This study highlights the importance of initial community assembly and its impact on the maturation trajectory of the infant gut microbiota.
Review
Food Science & Technology
Guillermo Ortiz Charneco, Paul P. de Waal, Irma M. H. van Rijswijck, Noel N. M. E. van Peij, Douwe van Sinderen, Jennifer Mahony
Summary: Bacteriophages pose a persistent threat to food fermentations, particularly large-scale commercial dairy fermentations. The interaction between phages and lactic acid bacteria used as starter cultures in dairy fermentations has been extensively studied, leading to advances in understanding coevolution and the development of robust starter cultures. This review highlights recent progress in phage-host interactions and phage resistance mechanisms in different bacterial species, and discusses their impact on the dairy fermentation industry and future plant-based food fermentations.
ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Giulia Alessandri, Federico Fontana, Chiara Tarracchini, Sonia Mirjam Rizzo, Massimiliano G. Bianchi, Giuseppe Taurino, Martina Chiu, Gabriele Andrea Lugli, Leonardo Mancabelli, Chiara Argentini, Giulia Longhi, Rosaria Anzalone, Alice Viappiani, Christian Milani, Francesca Turroni, Ovidio Bussolati, Douwe van Sinderen, Marco Ventura
Summary: In this study, a B. longum subsp. longum strain with a high similarity to the human gut-associated B. longum subsp. longum taxon was identified and its interactomic features with the host and other intestinal microbes were investigated using in vitro models, revealing extensive cross-talk.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Guillermo Ortiz Charneco, Philip Kelleher, Andrius Buivydas, Sofia Dashko, Paul P. de Waal, Noel N. M. E. van Peij, Richard J. Roberts, Jennifer Mahony, Douwe van Sinderen
Summary: In this study, it was found that the fermented meat isolate Lactococcus lactis subsp. lactis UC11 harbors a 49.3-kb plasmid called pUC11B, which encodes a pMRC01-like conjugation system and tetracycline resistance. Mutational analysis of the genes in the presumed pUC11B conjugation cluster revealed the presence of essential conjugation genes and a transcriptional repressor gene called trsR. Additionally, the plasmid pUC11B encodes an anti-restriction protein, TrsAR, which enhances conjugation frequencies in recipient strains with Type II or Type III RM systems.
MICROBIAL BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Silvia Ruta, Matthew Murray, Zoe Kampff, Brian McDonnell, Gabriele Andrea Lugli, Marco Ventura, Massimo Todaro, Luca Settanni, Douwe van Sinderen, Jennifer Mahony
Summary: In this study, the microbial ecology of milk, curd, and whey from five Pecorino Siciliano PDO-producing farms in Sicily were evaluated using metagenomic and microbiological approaches. The diversity of dairy lactococcal and streptococcal isolates, including newly described phage-resistance systems, was analyzed through genotyping tools and whole genome sequencing.
FERMENTATION-BASEL
(2023)
Article
Microbiology
Chiara Argentini, Chiara Tarracchini, Giulia Alessandri, Giulia Longhi, Christian Milani, Douwe van Sinderen, Marco Ventura, Francesca Turroni
Summary: In this study, it was found that increased exopolysaccharide (EPS) production by bifidobacteria is associated with enhanced resistance to beta-lactam antibiotics. The genes and expression patterns related to EPS production were also explored. This study provides experimental evidence on how bifidobacterial EPS modulates antibiotic susceptibility.
FEMS MICROBIOLOGY ECOLOGY
(2023)
