Article
Chemistry, Applied
Jin-Long Tian, Xu Si, Yue-Hua Wang, Er-Sheng Gong, Xu Xie, Ye Zhang, Bin Li, Chi Shu
Summary: This study established an optimized method for extracting and enriching flavonoids from R. corchorifolius fruit, isolating and identifying 12 flavonoids, six of which were obtained for the first time from R. corchorifolius. Compound 4 in particular showed significant inhibitory effects on alpha-glucosidase and alpha-amylase, with molecular modeling revealing its strong binding with the active sites of the enzymes. The enriched flavonoids and compound 4 demonstrated significant hypoglycemic effects in mice, highlighting the potential value of R. corchorifolius fruit flavonoids as dietary supplements.
Article
Biology
Azad Farzadfard, Jannik Nedergaard Pedersen, Georg Meisl, Arun Kumar Somavarapu, Parvez Alam, Louise Goksoyr, Morten Agertoug Nielsen, Adam Frederik Sander, Tuomas P. J. Knowles, Jan Skov Pedersen, Daniel Erik Otzen
Summary: Farzadfard et al. conducted a comprehensive analysis on a range of C-terminal truncations of aSN, revealing the importance of high C-terminus charge for decreased fibrillation rates. Truncated aSN showed decreased ability to form oligomers, disrupt synthetic vesicles, and exhibit cell toxicity, while still promoting or inhibiting aggregation through intramolecular interactions. Truncation of the C-terminal domain not only increased fibrillation tendency but also suppressed toxic oligomer formation, with changes in electrostatic potential serving as a double-edged safety guard.
COMMUNICATIONS BIOLOGY
(2022)
Article
Neurosciences
Ethan W. Hass, Zachary A. Sorrentino, Yuxing Xia, Grace M. Lloyd, John Q. Trojanowski, Stefan Prokop, Benoit Giasson
Summary: Studies demonstrate significant differences in carboxy terminal alpha Syn processing associated with pathological inclusions in different disease types, brain regions, and cell populations.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2021)
Article
Energy & Fuels
Yujunjie Zhou, Jiajie Wang, Xun Wang, Fei Wang, Xun Li
Summary: In this study, the synthesis of a-pinene in Escherichia coli was optimized through the discovery of key enzymes and the fine-tuning of fermentation conditions. The results demonstrated the potential for large-scale production of a-pinene through microbial de novo synthesis.
Article
Chemistry, Physical
Mozhgan Razzaghi, Ahmad Homaei, Roohullah Hemmati, Dariush Saberi, Soudabeh Kavousipour
Summary: Cellulose nanocrystals/gold nanoparticle hybrids were synthesized using hydrothermal treatment without toxic chemicals. The immobilized enzyme showed increased optimal temperature and retained higher activity and efficiency under extreme conditions.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Review
Biotechnology & Applied Microbiology
Prayatna Sharma, Krishnendu Mondal, Keshab Chandra Mondal, Nagendra Thakur
Summary: With the development of green chemistry, the use of enzymes in industrial processes as an alternative to traditional chemical catalysts has gained significant attention. Among these enzymes, alpha-amylase stands out due to its important role in starch hydrolysis and biotechnological applications. Various methods have been devised to improve the thermostability of enzymes in response to industrial demand, including protein engineering, directed evolution, and enzyme immobilisation strategies.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Neurosciences
Ana Martinez Hernandez, Ivan Silbern, Insa Geffers, Lars Tatenhorst, Stefan Becker, Henning Urlaub, Markus Zweckstetter, Christian Griesinger, Gregor Eichele
Summary: The article introduces two mouse models of synucleinopathies based on mutations in alpha-synuclein that do not overexpress the mutant protein, with a triple alanine to proline mutation and a C-terminal truncation. These models can be used to study the pathology associated with alpha-synuclein variants.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Spectroscopy
Yao Chen, Jin Yan, Xiaozhi Wang, Siwei Zhang, Jun Li, Ying Tang, Tong Wang
Summary: A fluorescence sensing method was developed for the quantification of alkaline phosphatase (ALP) by integrating strand displacement amplification with DNAzyme-catalytic recycling cleavage of molecular beacons. The proposed method achieved sensitive and specific ALP detection in human serum samples, showing potential for application in biomedical research and clinical diagnosis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Nutrition & Dietetics
Shuangshuang Li, Wenjing Wu, Jing Li, Shengnan Zhu, Xi Yang, Lijun Sun
Summary: In this study, the courses of starch digestion with individual alpha-amylase (AA), amyloglucosidase (AMG), and AA/AMG bi-enzyme system were analyzed. It was found that AMG had higher catalytic efficiency for starchy-substrates with more complex structures, resulting in a faster rate of glucose production compared to the AA/AMG bi-enzyme system. Tannic acid (TA) showed a higher binding affinity to AA, affecting the thermal stability of the enzyme, but not its inhibitory effects. The binding property of TA to AA/AMG bi-enzyme system resulted in more free AMG, leading to increased and faster glucose production during the digestion of slowly digestible starch (SDS).
FRONTIERS IN NUTRITION
(2022)
Article
Microbiology
Pashupati Bhandari, Jeffrey Tingley, D. Wade Abbott, Janet E. Hill
Summary: Gardnerella spp. are able to produce enzymes that can digest glycogen, which serves as a food source for vaginal bacteria. This study provides insights into the enzyme activity of Gardnerella spp.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Olga Dlugosz, Julia Matysik, Wiktoria Matyjasik, Marcin Banach
Summary: The study found that alpha-amylase immobilized on Fe3O4 nanoparticles maintained high catalytic activity and can be used as a catalyst in the process of starch hydrolysis. The most effective parameters of alpha-amylase immobilization on zinc oxide and Fe3O4 nanoparticles were determined.
JOURNAL OF CLUSTER SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Avijit Sardar, Tapas Bera, Santosh Kumar Samal, Nikesh Dewangan, Mithila Kamble, Samit Guha, Pradip K. Tarafdar
Summary: The spike protein (S protein) of SARS-CoV-2 plays a critical role in the fusion between viral and host membranes during infection. Recent experiments have shown that the fusion peptide (FP) is unable to modulate fusion kinetics at low peptide-to-lipid ratios, similar to the density of spikes on the viral surface. However, by modifying the C-terminus of the FP and attaching a myristoyl chain (C-myr-FP), fusion can be greatly accelerated, suggesting the importance of myristoylation and the N-terminus. C-myr-FP enhances murine coronavirus infection by promoting syncytia formation in L2 cells, indicating its potential use in biomedical applications.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Applied
Jiayi Li, Xinjing Kong, Yongfeng Ai
Summary: Maltogenic alpha-amylase (MGA) was used to modify starches for improved functionality. MGA treatment reduced retrogradation and viscosity of the starches, increased resistant starch content in NMS, and had limited influence on HAMS functionality. These findings are important for developing starch ingredients with enhanced functionality.
CARBOHYDRATE POLYMERS
(2022)
Article
Food Science & Technology
Nima Babolanimogadam, Hassan Gandomi, Afshin Akhondzadeh Basti, Mohammad J. Taherzadeh
Summary: This study evaluated the effects of different treatments of oat slurry on the nutritional, functional, and sensorial properties of oat milk. The results showed that the different treatments had different effects on the oat milk, with the two-stage treatments being more effective in terms of nutrition and functionality.
FOOD SCIENCE & NUTRITION
(2023)
Article
Engineering, Environmental
Lingzhu Chen, Yongjun Liu, Xue Fang, Yan Cheng
Summary: A strategy for large-scale production of high-activity alpha-MnO2 catalysts in VOCs oxidation is crucial. By adjusting the precipitation temperature, construction of oxygen vacancies on alpha-MnO2 has been achieved, improving its catalytic activity. The best catalyst, alpha-MnO2-60, showed significantly improved activity despite its lower specific surface area, highlighting the importance of surface oxygen vacancies in catalytic activity. This work offers insights for rational design of efficient VOCs catalysts.
JOURNAL OF HAZARDOUS MATERIALS
(2021)