期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 80, 期 1, 页码 354-359出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02987-13
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资金
- National Center for Research Resources, National Institute of Health [UL1TR000083]
- National Center for Advancing Translational Sciences, National Institute of Health [UL1TR000083]
- North Carolina State University Center for Comparative Molecular Medicine and Translational Research [2011-2611]
- NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES [UL1TR000083] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [P30DK034987] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM007092] Funding Source: NIH RePORTER
Ticks are important vectors for many emerging pathogens. However, they are also infected with many symbionts and commensals, often competing for the same niches. In this paper, we characterize the microbiome of Amblyomma americanum (Acari: Ixodidae), the lone star tick, in order to better understand the evolutionary relationships between pathogens and nonpathogens. Multitag pyrosequencing of prokaryotic 16S rRNA genes (16S rRNA) was performed on 20 lone star ticks (including males, females, and nymphs). Pyrosequencing of the rickettsial sca0 gene (also known as ompA or rompA) was performed on six ticks. Female ticks had less diverse microbiomes than males and nymphs, with greater population densities of Rickettsiales. The most common members of Rickettsiales were Candidatus Rickettsia amblyommii and Candidatus Midichloria mitochondrii. Ca. Rickettsia amblyommii was 2.6-fold more common in females than males, and there was no sequence diversity in the sca0 gene. These results are consistent with a predominantly vertical transmission pattern for Ca. Rickettsia amblyommii.
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