Article
Biotechnology & Applied Microbiology
Liu-Jing Wei, Xuan Cao, Jing-Jing Liu, Suryang Kwak, Yong-Su Jin, Wei Wang, Qiang Huaa
Summary: This study demonstrated a novel metabolic design for enhancing squalene accumulation in Y. lipolytica by overexpressing mevalonate pathway enzymes, increasing lipid production, and deleting nitrogen catabolite repression-related genes. The results suggest that deletion of URE2 can be utilized to boost hydrophobic target product production in oleaginous yeast strains. This discovery holds potential for future engineering of cell factories to efficiently produce terpenoids.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
Caleb Walker, Bruce Dien, Richard J. Giannone, Patricia Slininger, Stephanie R. Thompson, Cong T. Trinh
Summary: The study analyzed genetic diversity of undomesticated Y. lipolytica strains, identified genes associated with desirable phenotypes, and compared the differences in xylose utilization and lipid accumulation between these strains and the conventional strain. Proteomic analysis revealed metabolic processes and regulatory elements influencing growth, sugar utilization, and lipid accumulation in undetoxified biomass hydrolysates by natural variants.
Article
Biochemistry & Molecular Biology
Marek Kieliszek, Marianna Dourou
Summary: This study evaluated the effects of selenium on the growth and lipid production of two Yarrowia lipolytica strains and found that selenium negatively affected cell mass production and total lipid accumulation, leading to metabolic disorders and impacting cell morphology and lipid droplet distribution. Additionally, selenium influenced fatty acid composition differently in the two strains.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Energy & Fuels
Polinati Satya Sagar, Satish Kommoji, Iyyappan Jayaraj, Deepanraj Balakrishnan, Feroz Shaik, Mahendra Vucha, Lalitha Gnanasekaran
Summary: This study investigated the optimization of thermal-diluted acid pretreatment on native grass biomass for simple sugar recovery, followed by lipid bioproduction using Yarrowia lipolytica. The maximum total reducing sugar (34.26 +/- 1.55 g/L) in the native grass hydrolysate was obtained at 163.8 degrees C, 1.57% (v/v) sulfuric acid concentration for 21 min. Detoxified native grass hydrolysate yielded 11.64 +/- 0.84 g/L of lipid with 65.52% (w/w) lipid content after 96 h using Y. lipolytica. These results highlight the efficiency of optimizing thermal-diluted acid pretreatment on native grass biomass for lipid production using Y. lipolytica.
Article
Biochemistry & Molecular Biology
Naghmeh Poorinmohammad, Jing Fu, Bob Wabeke, Eduard J. Kerkhoven
Summary: This study investigated the molecular mechanism underlying lipid accumulation in Yarrowia lipolytica through proteomic analysis. Various cellular functions and biological mechanisms related to lipid accumulation were identified, and genetic engineering of selected targets was used to validate their effects on lipid content, providing new strategies to optimize lipid yield.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Yongshuo Ma, Nian Liu, Per Greisen, Jingbo Li, Kangjian Qiao, Sanwen Huang, Gregory Stephanopoulos
Summary: This study reports the removal of lycopene substrate inhibition in Yarrowia lipolytica by two different strategies, resulting in high carotenoid productivity. The findings provide effective approaches for efficient synthesis of natural products.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Veronika Urbanikova, Young-Kyoung Park, Daniela Krajciova, Mehdi Tachekort, Milan Certik, Ioana Grigoras, Roman Holic, Jean-Marc Nicaud, Peter Gajdos
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was used as a host for the production of Punicic acid (PuA). By supplementing the medium with pomegranate seed oil, lipids containing PuA esters were accumulated up to 31.2% in Y. lipolytica. Strategies involving the expression of the bifunctional fatty acid conjugase/desaturase gene from Punica granatum (PgFADX) allowed for de novo production of PuA in Y. lipolytica. The optimized promoter for PgFADX expression resulted in a PuA production of 36.6 mg/L. These findings highlight the potential of Y. lipolytica as a promising host for PuA production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biotechnology & Applied Microbiology
Young-Kyoung Park, Rodrigo Ledesma-Amaro
Summary: Yarrowia lipolytica is a promising candidate for industrial bioproduction of chemicals, fuels, foods, and pharmaceuticals, thanks to its natural and engineered traits. In recent years, extensive research has been conducted to assess its potential, improve its features, and scale up its processes. The increasing number of publications and patents related to Y. lipolytica demonstrates its importance and potential in the field. Despite the remaining challenges, it is expected that Y. lipolytica will become one of the preferred choices for industrial applications in the near future.
TRENDS IN BIOTECHNOLOGY
(2023)
Review
Biochemical Research Methods
Yu-Lei Jia, Jin Li, Fang-Tong Nong, Chun-Xiao Yan, Wang Ma, Xiao-Feng Zhu, Li-Hui Zhang, Xiao-Man Sun
Summary: Adaptive laboratory evolution (ALE) plays a crucial role in microbial breeding by simulating natural evolution process and rapidly obtaining strains with stable traits. It provides a powerful tool for constructing stable microbial cell factories and has been widely used in improving target product synthesis, expanding substrate utilization range, and enhancing chassis cells' tolerance. ALE also employs stress strategies to enhance the production of target compounds.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Microbiology
Hui Wei, Wei Wang, Eric P. Knoshaug, Xiaowen Chen, Stefanie Van Wychen, Yannick J. Bomble, Michael E. Himmel, Min Zhang
Summary: This study established a new cellulase coexpressing platform in Y. lipolytica by genetic modifications, which improved lipid accumulation and cellulase activity, reduced endoplasmic reticulum stress, and provided a potent tool for further development of Y. lipolytica as a robust host for the expression of cellulases and other commercially important proteins.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sonia Abreu, Young-Kyoung Park, Camilla Pires de Souza, Lea Vidal, Pierre Chaminade, Jean-Marc Nicaud
Summary: Yarrowia lipolytica is a promising yeast for producing unusual lipids. Recent studies have shown that optimizing substrate combination and metabolic pathways can improve lipid production. In this study, the lipid composition of obese LP and obese L strains was compared, revealing differences in the fatty acid composition and molecular species distributions of various lipids.
Article
Agricultural Engineering
Satish Kommoji, M. Gopinath, Polinati Satya Sagar, D. Yuvaraj, J. Iyyappan, A. Jaya Varsha, Varsha Sunil
Summary: In this study, lipid production using native grass as a substrate with Yarrowia lipolytica MTCC 9519 was investigated. The results showed promising growth performance and lipid yield of Y. lipolytica MTCC 9519 when utilizing pretreated native grass hydrolysate, suggesting the potential of native grass as a substrate for biolipid production.
BIORESOURCE TECHNOLOGY
(2021)
Article
Environmental Sciences
Soodeh Salimi Khaligh, Ece Polat, Mahmut Altinbas
Summary: This study aims to increase cell growth and lipid accumulation of Yarrowia lipolytica under nitrogen deficiency condition by using food waste as a carbon source. Two-stage batch strategy was used with fermented food waste as initial carbon source and three different carbon sources (glucose, glycerol, and potassium acetate) in the second stage. The best results were obtained with glycerol at a COD/TKN ratio of 125, achieving a high lipid content of 42.2% and biomass concentration of 12.95 g/L.
WASTE AND BIOMASS VALORIZATION
(2023)
Article
Biotechnology & Applied Microbiology
Yongshuo Ma, Jingbo Li, Sanwen Huang, Gregory Stephanopoulos
Summary: This study explores the strategy of expressing the astaxanthin biosynthesis pathway in sub-organelles of the oleaginous yeast Yarrowia lipolytica to achieve efficient synthesis of astaxanthin, leading to increased yield and reduced accumulation of intermediates. Targeting the astaxanthin pathway to subcellular organelles accelerates conversion and significantly improves synthesis, ultimately enhancing compartmentalized isoprenoid biosynthesis in Y. lipolytica.
METABOLIC ENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Agata Fabiszewska, Magdalena Paplinska-Goryca, Paulina Misiukiewicz-Stepien, Malgorzata Woloszynowska, Dorota Nowak, Bartlomiej Zieniuk
Summary: Yarrowia lipolytica yeast is a model species capable of accumulating intracellular lipids. The synthesis of lipids can follow either a de novo pathway or an ex novo pathway. The ex novo pathway is influenced by the limitation of nitrogen source in the medium. It was also found that lipid synthesis in lipid-rich medium mainly occurs via the ex novo pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)