Review
Chemistry, Analytical
Poramin Boonbanjong, Kiatnida Treerattrakoon, Wassa Waiwinya, Piyawat Pitikultham, Deanpen Japrung
Summary: Isothermal amplification (IA) is a nucleic acid amplification technology that has significantly contributed to the healthcare system by improving disease diagnosis through higher sensitivity, specificity, and rapidity. Certain IA techniques provide a rapid, sensitive, specific, and simple method for disease diagnosis. However, not all IA techniques have been widely used in clinical applications due to the influence of different biomarkers and sample types on the IA system.
Article
Chemistry, Analytical
Hyo Eun Kim, Ariadna Schuck, Hyeonseek Park, Hee Jae Huh, Minhee Kang, Yong-Sang Kim
Summary: The electrochemical analysis of SARS-CoV-2 using a screen-printed carbon electrode (SPCE) coupled with a thin-film heater based on the loop-mediated isothermal amplification (LAMP) technique successfully detected the envelope (E) and RNA-dependent RNA polymerase (RdRP) genes. The SPCE sensor with synthesized gold nanostars (AuNSs) showed improved sensitivity, and the optimized LAMP assay using a real-time amplification reaction system accurately analyzed both positive and negative clinical samples. The developed device has potential as a point-of-care test DNA-based sensor for the diagnosis of SARS-CoV-2.
Article
Plant Sciences
R. Logeshwari, C. Gopalakrishnan, A. Kamalakannan, J. Ramalingam, R. Saraswathi
Summary: A rapid and highly specific detection method based on LAMP technology was developed in this study for the seed-borne pathogen Sarocladium oryzae. This method offers quick and reliable detection of the pathogen, which is important for maintaining the health of rice seeds.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Chemistry, Analytical
Meng Chen, Qidi He, Yanli Tong, Zuanguang Chen
Summary: This study successfully combined LAMP with RCA to achieve rapid detection and quantification of pathogens. By using a strand displacement strategy in a single-tube reaction, different pathogen target genes were successfully detected, demonstrating the universality and reliability of the method.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Entomology
Yue Zhang, Weisong Li, Massimiliano Virgilio, Marc De Meyer, Zhihong Li
Summary: This study achieved rapid molecular identification of Ceratitis fruit flies using LAMP technology and mitochondrial genes as targets. A loop-mediated isothermal amplification identification technology system was established, which utilized rapid, rough DNA extraction and visual detection. The system demonstrated high sensitivity and simplicity, allowing the entire identification process to be completed within one hour.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Chemistry, Analytical
Huan-Huan Zhu, Yuan Li, Li-Xia Wu, Ke-Sheng Wang, Yue Zhang, Qiang-Yuan Fan, Zun-Zhen Ming, Wei-Qin Chen, Wei-Wei Liu
Summary: Loop-mediated isothermal amplification (LAMP) is a promising diagnostic tool for genetic amplification, known for its speed, simplicity, efficiency, and sensitivity. This research introduces an internal heating method for LAMP using magnetic nanoparticles (MNPs) called nano-LAMP, which can detect low concentrations of HPV and has been validated in clinical samples.
Article
Fisheries
Shun Zhou, Qing Huang, Mingming Yu, Ying Li, Xujia Zheng, Yunji Xiu
Summary: The study developed a rapid detection method, LAMP-HNB assay, for Aeromonas salmonicida, an important pathogen in fish farming, with good specificity and high sensitivity. The method has potential applications in diagnostic testing, health certification, and surveillance programs.
JOURNAL OF FISH DISEASES
(2021)
Article
Medicine, Legal
Seiji Kubo, Keito Amai, Jin Tanaka, Hideki Niimi
Summary: This study reports a one-tube, two-step isothermal amplification assay for rapid and sensitive screening of saliva samples. The assay can be performed in a closed tube and the products are detected using real-time fluorescence analysis. The strategy shows promise for screening saliva from forensic samples.
FORENSIC SCIENCE INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Tadafumi Yokoyama, Yuko Tasaki, Natsumi Inoue, Naotoshi Sugimoto, Eri Nariai, Sanae Kuramoto, Taizo Wada
Summary: This study developed a RT-LAMP technique for the diagnosis of HPeVs, particularly HPeV3, allowing for rapid and convenient detection of HPeV3 RNA. The technique showed clinical utility in diagnosing newborns and infants with sepsis-like symptoms.
Review
Microbiology
Cassandra Suther, Sloane Stoufer, Yanjiao Zhou, Matthew D. Moore
Summary: Foodborne and enteric viruses impose a significant burden on public health and economy globally. Real time PCR, although considered a gold standard, has limitations in terms of portability, sample processing, and time to result. Isothermal amplification methods have shown promise as portable, sensitive, and rapid alternatives to PCR for detecting these viruses.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Review
Chemistry, Analytical
Jesse M. M. Marangoni, Kenneth K. S. Ng, Arezoo Emadi
Summary: Loop-mediated isothermal amplification (LAMP) is a useful tool for pathogen detection in clinical and environmental samples, but monitoring LAMP can be challenging for point-of-use applications. This review discusses the use of voltammetric sensors for LAMP monitoring and highlights various strategies for detecting DNA amplicons, pyrophosphate, and protons. The review also addresses the hurdles in adapting these sensors for on-site applications.
Review
Plant Sciences
Sandra V. Gomez-Gutierrez, Stephen B. Goodwin
Summary: LAMP is a molecular technique for detection of plant pathogens, known for its specificity, sensitivity, and ability to conduct reactions at a constant temperature. This review explains the amplification process, visualization techniques, advantages, and disadvantages of LAMP compared to alternative isothermal approaches. It also presents the application of LAMP in detecting fungal pathogens and viruses in wheat, as well as the development of real-time and multiplex LAMP assays.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Chemistry, Analytical
Jocelyn Reynolds, Reid S. Loeffler, Preston J. Leigh, Hannah A. Lopez, Jeong-Yeol Yoon
Summary: Isothermal nucleic acid amplification tests, which only require a constant temperature and simplify the amplification process, have gained popularity over PCR. Paper microfluidics have been extensively used for automated nucleic acid extraction, amplification, and detection in these isothermal amplification tests.
Article
Biochemical Research Methods
Xuliang Zhang, Yingfeng Zhao, Chang Ma, Wei Wu, Min Dong, Jinwei You, Jie Liu, Shifeng Yun
Summary: In this study, a rapid and visual detection method for minute virus of mice (MVM) using loop-mediated isothermal amplification (LAMP) with hydroxynaphthol blue (HNB) dye was developed. The assay showed high sensitivity and specificity, with a lower limit of detection compared to conventional PCR. The established LAMP system was successfully applied to detect MVM infections in laboratory animal samples.
JOURNAL OF VIROLOGICAL METHODS
(2022)
Article
Entomology
Matteo Marchioro, Massimo Faccoli
Summary: The Walnut Twig Beetle (WTB) and the pathogen causing thousand cankers disease (TCD) pose serious threats to walnut orchards in Europe. Factors affecting WTB dispersal were analyzed using a model developed from 8 years of monitoring data, which also allows for assessing the colonization risk of specific walnut orchards.
JOURNAL OF PEST SCIENCE
(2022)
Review
Entomology
Sofia Branco, Massimo Faccoli, Eckehard G. Brockerhoff, Geraldine Roux, Herve Jactel, Nicolas Desneux, Emmanuel Gachet, Raphaelle Mouttet, Jean-Claude Streito, Manuela Branco
Summary: Efforts have been made to prevent Asian longhorn beetles from causing harm to forest and urban trees, with over 45% of eradication programmes successful in the last 12 years. Despite the high costs of eradication programs, the benefits outweigh the costs. Eradication efforts for Anoplophora chinensis are more challenging compared to Anoplophora glabripennis.
JOURNAL OF PEST SCIENCE
(2022)
Article
Entomology
Andrea Battisti, Jose A. Hodar, Rodolfo Hernandez, Stig Larsson
Summary: This study examines the relationship between population density and egg mass aggregation in two species of pine processionary moths. The results show that egg mass aggregation deviates from uniform distribution at high population density, which may have significant implications for insect outbreaks.
ECOLOGICAL ENTOMOLOGY
(2023)
Article
Entomology
Davide Scaccini, Davide Bramuzzo, Cengiz Bostanci, Massimo Faccoli, Isabel Martinez-Sanudo, Alexey Matov, Alberto Zilli, Alberto Pozzebon
Summary: The Asian walnut moth (Garella musculana) was found for the first time in NE Italy in 2021, marking its first record in the country and Western Europe. The species was identified based on morphology and genetic analysis, but further research is needed to clarify its classification due to similarities with other taxa. Resolving this issue is crucial for the identification and proper management of walnut moths.
JOURNAL OF APPLIED ENTOMOLOGY
(2023)
Article
Plant Sciences
Colton D. Meinecke, Lieschen De Vos, Neriman Yilmaz, Emma T. Steenkamp, Michael J. Wingfield, Brenda D. Wingfield, Caterina Villari
Summary: Fusarium circinatum, a pathogen that causes pine pitch canker, poses a global invasive threat. A molecular test using loop-mediated isothermal amplification (LAMP) technology was developed to accurately diagnose and monitor this pathogen in real-time. The assay has the potential to reduce the spread and impact of pitch canker worldwide.
Article
Zoology
Livio Mola, Enrico Ruzzier, Claudio Deiaco, Mauro Agosti, Massimo Faccoli, Jan Matejicek, Laura Farina, Luciano Diotti, Francesca Della Rocca
Summary: The Po Plain in Northern Italy is highly urbanized, but still contains fragmented oak-hornbeam forests, which serve as important refuges for both sedentary and migratory species. This paper presents a preliminary list of beetle species inhabiting the Monte Netto Regional Park, identified through 10 years of field surveys and various sampling techniques. A total of 834 species were identified, including non-native species and endangered species on the Red List. This survey highlights the conservation value of Monte Netto and the naturalness of its remaining forest patches.
Article
Forestry
Aurora Bozzini, Saverio Francini, Gherardo Chirici, Andrea Battisti, Massimo Faccoli
Summary: Extreme weather events are causing an increase in forest-pest outbreaks, with a windthrow event in 2018 in the southern Italian Alps causing a shift in populations of the European spruce bark beetle. This study used remote sensing techniques and a random forest model to detect outbreak spots in the southeast Alps. The model achieved an overall accuracy of 72% in 2022 and 58% in 2021, demonstrating the potential to locate even small outbreak areas or areas with mixed healthy and infested trees.
Article
Multidisciplinary Sciences
Enrico Ruzzier, Giacomo Ortis, Davide Vallotto, Massimo Faccoli, Isabel Martinez-Sanudo, Matteo Marchioro
Summary: Xyleborini is the largest tribe of Scolytinae with about 1300 species worldwide, all of which are primarily xylomycetophagous and develop on symbiotic fungi in plant woody tissues. The wood-boring action of Xyleborini, combined with the inoculation of symbiotic fungi, can cause host plant dieback, wood damage, and death, making many Xyleborini major pests on various trees. Importation of host plants is often a primary pathway for introducing and establishing Xyleborini species in non-native environments, and efforts are being made to manage their invasive potential. However, a lack of data on Xyleborini host plants hinders the development of effective detection and monitoring strategies and risk assessment for plant pests and invasive species. This study provides updated host records and economic categorization for the 1293 known Xyleborini species worldwide.
Article
Entomology
Julianne Farrell, Myron P. Zalucki, Andrea Battisti
Summary: Host specificity of an Australian processionary moth species was studied using reciprocal transplant experiments. The moth, Ochrogaster lunifer, has genetically distinct nesting forms associated with acacias and eucalypts. The study confirmed genetic separation and the importance of host specialization in the speciation process.
Article
Food Science & Technology
Claude Bragard, Paula Baptista, Elisavet Chatzivassiliou, Francesco Di Serio, Josep Anton Jaques Miret, Annemarie Fejer Justesen, Alan MacLeod, Christer Sven Magnusson, Panagiotis Milonas, Juan A. Navas-Cortes, Stephen Parnell, Roel Potting, Philippe Lucien Reignault, Emilio Stefani, Hans-Hermann Thulke, Wopke Van der Werf, Antonio Vicent Civera, Jonathan Yuen, Lucia Zappala, Andrea Battisti, Hugo Mas, Massimo Faccoli, Ciro Gardi, Alzbeta Mikulova, Olaf Mosbach-Schulz, Giuseppe Stancanelli, Fabio Stergulc, Paolo Gonthier
Summary: The European Commission has proposed the use of sulfuryl fluoride on ash log shipments for phytosanitary certification. The Panel on Plant Health conducted a quantitative assessment on the likelihood of pest freedom from A. planipennis for two different commodities fumigated with sulfuryl fluoride. They concluded that the likelihood of pest freedom is lower for ash logs with bark compared to debarked ash logs.
Article
Entomology
Luca Deganutti, Filippo Biscontin, Iris Bernardinelli, Massimo Faccoli
Summary: This study investigated the large-scale use of a semiochemical push-and-pull strategy to protect vulnerable Norway spruce edge trees from the European spruce bark beetle after a windthrow disturbance. The results showed that the push-and-pull technique was effective in reducing damage to forest edges in high-risk areas. However, its effectiveness was limited in clear-cut areas, where it did not significantly reduce the number of infested trees along the forest edge.
AGRICULTURAL AND FOREST ENTOMOLOGY
(2023)
Article
Entomology
Mizuki Uemura, Myron P. Zalucki, Andrea Battisti
Summary: This study observed the tent construction and foraging behavior of Thaumetopoea pityocampa caterpillars and found differences among individuals in terms of sex, body size, and larval stage. The findings highlight the importance of communal living as a defense against environmental elements and natural enemies, and provide insights for further investigation in polyethism.
Article
Forestry
Davide Nardi, Aurora Bozzini, Giuseppe Morgante, Angelo Gaccione, Valerio Finozzi, Andrea Battisti
Summary: During pest outbreaks, mapping tools are crucial for accurate detection. Participatory projects can provide ground data with high accuracy in detecting early-stage infestations and small spots. However, satellite approaches are essential for estimating infestations due to spatial bias in ground data. This study shows how a participatory approach involving nonspecialized staff and a GIS-based app can provide complementary ground truth data to satellite data.
ANNALS OF FOREST SCIENCE
(2023)
Article
Microbiology
Juan Carlos Cambronero-Heinrichs, Andrea Battisti, Peter H. W. Biedermann, Giacomo Cavaletto, Victor Castro-Gutierrez, Lorenzo Favaro, Giacomo Santoiemma, Davide Rassati
Summary: Ambrosia beetles have abundant bacteria in their active galleries, which play a crucial role in providing nutrition and defense. This study supports the hypothesis of mutualistic interaction between Erwiniaceae bacteria and ambrosia beetles.
FEMS MICROBIOLOGY ECOLOGY
(2023)