☆ 4.6 Article

Improved Bacterial Mutagenesis by High-Frequency Allele Exchange, Demonstrated in Clostridium difficile and Streptococcus suis

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2013)

期刊

APPLIED AND ENVIRONMENTAL MICROBIOLOGY

卷 79, 期 15, 页码 4768-4771

出版社

AMER SOC MICROBIOLOGY

DOI: 10.1128/AEM.01195-13

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Biotechnology & Applied Microbiology Microbiology

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Medical Research Council [94717]
Biotechnology and Biological Sciences Research Council [BB/G019177/1]
BBSRC [BB/G019177/1] Funding Source: UKRI
MRC [G1000214, MR/K000551/1] Funding Source: UKRI
Biotechnology and Biological Sciences Research Council [BB/G019177/1] Funding Source: researchfish
Medical Research Council [MR/K000551/1, G1000214] Funding Source: researchfish

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摘要

Here we show that the frequency of mutant isolation by two-step allele exchange can be improved by increasing the length of homologous DNA and the opportunity for recombination, obviating the need for counterselection markers. These principles are demonstrated in Clostridium difficile and Streptococcus suis but are likely to be generally applicable.

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