期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 78, 期 16, 页码 5788-5795出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00895-12
关键词
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资金
- Spanish Government [CGL2011-25401]
- research program of the Xarxa de Referencia en Biotecnologia (XRB), Government of Catalonia
- research program of the Comissionat per a Universitats i Recerca del DIUE, Government of Catalonia [2009SGR1043]
- grant FI from the Government of Catalonia [2009SGR1043]
Bifidobacterium spp. belong to the commensal intestinal microbiota of warm-blooded animals. Some strains of Bifidobacterium show host specificity and have thus been proposed as host-specific targets to determine the origin of fecal pollution. Most strains have been used in microbial-source-tracking (MST) studies based on culture-dependent methods. Although some of these approaches have proved very useful, the low prevalence of culturable Bifidobacterium strains in the environment means that molecular culture-independent procedures could provide practical applications for MST. Reported here is a set of common primers and four Bifidobacterium sp. host-associated (human, cattle, pig, and poultry) probes for quantitative-PCR (qPCR) assessment of fecal source tracking. This set was tested using 25 water samples of diverse origin: urban sewage samples, wastewater from four abattoirs (porcine, bovine, and poultry), and water from a river with a low pollution load. The selected sequences showed a high degree of host specificity. There were no cross-reactions between the qPCR assays specific for each origin and samples from different fecal origins. On the basis of the findings, it was concluded that the host-specific qPCRs are sufficiently robust to be applied in environmental MST studies.
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