Identification and Distribution of High-Abundance Proteins in the Octopus Spring Microbial Mat Community

A shotgun metaproteomics approach was employed to identify proteins in a hot spring microbial mat community. We identified 202 proteins encompassing 19 known functions from 12 known phyla. Importantly, we identified two key enzymes involved in the 3-hydroxypropionate CO2 fixation pathway in uncultivated Roseiflexus spp., which are known photoheterotrophs.