Article
Food Science & Technology
Yuting Shang, Qinghua Ye, Qingping Wu, Rui Pang, Baoqing Zhou, Chufang Wang, Xinran Xiang, Fan Li, Juan Wang, Yinzhi Zhang, Jiasheng Wang, Xiulan Sun, Jumei Zhang
Summary: This study successfully identified species-specific molecular targets for different Cronobacter species using a bioinformatics approach and developed a multiplex PCR method for species identification. The PCR and mPCR assays showed excellent specificity and sensitivity for detecting Cronobacter species, suggesting their potential for use in food inspection and clinical diagnosis.
Article
Microbiology
Lu Wang, Pan Wu, Yingying Su, Yi Wei, Xi Guo, Lan Yang, Min Wang, Bin Liu
Summary: This study identified novel marker genes for the genus Cronobacter, as well as specific marker genes for three species within the genus. Duplex and multiplex PCR methods based on these markers were established and validated for accurate detection of the strains. The developed methods can be used as valuable tools for identifying these strains in various samples.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Li Sun, David G. Meckes
Summary: The new immuno-PCR method utilizes TotalSeq antibodies containing DNA conjugated oligos for high sensitivity and specificity profiling of multiple EV surface proteins simultaneously. This method has been successfully applied to cell and serum samples, and a micro-size exclusion chromatography method has been developed to detect EV surface proteins in as little as 10 μl of human serum when combined with immuno-PCR. Overall, this approach allows for rapid detection of multiple EV markers from small sample volumes in a single tube.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Mustafa Emin Oz, Emrah Torlak
Summary: In this study, a high-resolution melting (HRM) assay using EvaGreen was used to differentiate a vaccine strain of peste des petits ruminants virus (PPRV) from field isolates.
ARCHIVES OF VIROLOGY
(2022)
Article
Chemistry, Analytical
Kerou Zhang, Alessandro Pinto, Lauren Yuxuan Cheng, Ping Song, Peng Dai, Michael Wang, Luis Rodriguez, Cailin Weller, David Yu Zhang
Summary: In this study, we developed an occlusion system that functions as an efficient fluorescence indicator in HiFi qPCR reactions, improving the detection sensitivity for early cancer diagnosis and drug-resistance mutation identification.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Xu Chen, Yue Ma, Shuangyu Miao, Dongnan Li, Ye Zhang
Summary: In this study, a multiplex competitive annealing mediated isothermal amplification (mCAMP) assay on a microfluidic chip was developed for the visual detection of Cronobacter sakazakii in powdered infant formula (PIF) samples. The assay showed high specificity and a limit of detection of 2.2 x 10(3) CFU g(-1). The results are visible to the naked eye, making it suitable for rapid analysis in resource-limited settings.
Article
Agriculture, Dairy & Animal Science
Tohid Piri-Gharaghie, Ghazal Ghajari, Naz Tavakoli Lahijani, Renzon Daniel Cosme Pecho, Fahdil Hussam, Roxana Yolanda Castillo-Acobo, Mona Aghassizadeh-Sherbaf
Summary: This study aimed to develop a multiplex reverse transcriptase polymerase chain reaction (m-RT-PCR) method for simultaneous detection of important avian viral pathogens. Specific primers were designed and synthesized using conserved segments of viral genetic sequences, and recombinant vectors were synthesized as positive controls. The d-optimal approach was used to optimize PCR conditions. The m-PCR assay showed high specificity, sensitivity, and reproducibility in detecting and evaluating viral infection in birds, with the ability to detect 8 target genes and identify 8 viral pathogens from infected samples. The test also demonstrated that live animal oral swabs were as effective as tissue sampling from dead animals, making it a valuable tool for viral infection analysis in poultry.
Article
Parasitology
Woo Jun Bang, Min Hyeok Won, Seong Tae Cho, Jihun Ryu, Kwang Shik Choi
Summary: This study developed a rapid and simple multiplex PCR assay for six morphologically similar Aedini species in Korea. By generating species-specific fragments distinguishable by size, accurate identification of mosquito species was achieved, providing a useful tool for monitoring and controlling these vector mosquitoes.
PARASITES & VECTORS
(2021)
Article
Multidisciplinary Sciences
Yun Li, LuJie Zhao, Jingye Wang, Peipei Qi, Zhongfa Yang, Xiangyu Zou, Fujun Peng, Shengguang Li
Summary: The study aimed to establish a new method for rapid identification of common sepsis pathogens and evaluate its clinical application. A multiplex PCR assay was used to simultaneously amplify specific conserved regions of nine common pathogenic microorganisms. The assay showed high sensitivity and accuracy in detecting pathogens, making it useful for early initiation of effective antimicrobial treatment and routine clinical practice identification of sepsis pathogens.
Article
Food Science & Technology
Christian Brenn, Ute Schroeder, Reinhold Hanel, Pedro Martinez Arbizu
Summary: The study presents a TaqManprobe based multiplex real-time PCR screening assay for identification of four commercially relevant crustacean species, showing high specificity and potential for rapid species identification.
Article
Food Science & Technology
Na-Ye-Seul Kim, Eun-Ji Park, Seo-Hyun Lee, Kwang-Ho Mun, Ji-Young Yang, Jung-Beom Kim
Summary: Species-specific primers were developed to differentiate between tunas and billfishes, and a multiplex PCR assay was used to determine the authenticity of these species. The results showed that the developed primers were effective in distinguishing between the two species, making it a cost-effective technique for species identification.
FOOD SCIENCE AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Qing Fan, Zhixun Xie, You Wei, Yanfang Zhang, Zhiqin Xie, Liji Xie, Jiaoling Huang, Tingting Zeng, Sheng Wang, Sisi Luo, Meng Li
Summary: Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique that offers convenience, specificity, and sensitivity. However, previous LAMP detection methods have been limited to identifying a single target due to the interpretation methods. In this study, a modified LAMP method was developed that allows for the simultaneous detection of multiple target sequences.
Article
Multidisciplinary Sciences
Rungdawan Wongsamart, Parvapan Bhattarakasol, Arkom Chaiwongkot, Doonyapong Wongsawaeng, Pilailuk Akkapaiboon Okada, Tanapat Palaga, Asada Leelahavanichkul, Weerapan Khovidhunkit, Deborah Dean, Naraporn Somboonna
Summary: High rates of new cervical cancer cases and deaths are related to the limitation of regular cervical cancer screening in low- and middle-income countries. A new multiplex recombinase polymerase amplification assay was developed to detect 20 high-risk and 14 low-risk types of HPV, as well as specific genes related to cervical cancer development. The assay showed promising results in terms of specificity, diagnostic accuracy, speed, cost, and safety.
SCIENTIFIC REPORTS
(2023)
Article
Entomology
Sandthya Pramasivan, Jonathan Wee Kent Liew, Nantha Kumar Jeyaprakasam, Van Lun Low, Romano Ngui, Indra Vythilingam
Summary: This study aims to develop a quick and accurate method for identifying the important Anopheles vector species associated with simian malaria in Malaysia. Through molecular identification and multiplex PCR detection, the key Anopheles species were successfully identified. This method is valuable for accurate species identification, determining the correct vector, and understanding its geographical distribution.
Article
Biotechnology & Applied Microbiology
E. Kamra, D. Alam, V Singh, M. Kumar, M. Chauhan, P. K. Mehta
Summary: A multiplex-nested PCR (M-nested PCR) targeting mpt64 (Rv1980c) + IS6110 was designed for the detection of Mycobacterium tuberculosis (Mtb) DNA in urine, endometrial biopsies, and menstrual blood samples of UGTB patients. The M-nested PCR showed a lower detection limit and higher sensitivities compared to conventional M-PCR. Incorporation of an internal amplification control helped confirm true PCR-negative results. GeneXpert MTB/RIF assay had lower sensitivity but high specificity compared to M-nested PCR. The M-nested PCR shows promise as a potential diagnostic tool for UGTB.
LETTERS IN APPLIED MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Baoguang Li, Scott A. Jackson, Jayanthi Gangiredla, Weimin Wang, Huanli Liu, Ben D. Tall, Junia Jean-Gilles Beaubrun, Michele Jay-Russell, George Vellidis, Christopher A. Elkins
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Biotechnology & Applied Microbiology
Qiongqiong Yan, Juan Wang, Jayanthi Gangiredla, Yu Cao, Marta Martins, Gopal R. Gopinath, Roger Stephan, Keith Lampel, Ben D. Tall, Seamus Fanning
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2015)
Article
Microbiology
Monica Pava-Ripoll, Rachel E. Goeriz Pearson, Amy K. Miller, Ben D. Tall, Christine E. Keys, George C. Ziobro
Article
Biotechnology & Applied Microbiology
Lan Hu, Christopher J. Grim, Augusto A. Franco, Karen G. Jarvis, Vengopal Sathyamoorthy, Mahendra H. Kothary, Barbara A. McCardell, Ben D. Tall
Article
Biotechnology & Applied Microbiology
Qiongqiong Yan, Karen G. Jarvis, Hannah R. Chase, Karine Hebert, Larisa H. Trach, Chloe Lee, Jennifer Sadowski, Boram Lee, Seongeun Hwang, Venugopal Sathyamoorthy, Niall Mullane, Monica Pava-Ripoll, Carol Iversen, Franco Pagotto, Seamus Fanning, Ben D. Tall
Article
Biotechnology & Applied Microbiology
Shirley A. Micallef, Rachel E. Rosenberg Goldstein, Ashish George, Laura Ewing, Ben D. Tall, Marc S. Boyer, Sam W. Joseph, Amy R. Sapkota
Article
Biotechnology & Applied Microbiology
G. Gopinath, K. Hari, R. Jain, M. K. Mammel, M. H. Kothary, A. A. Franco, C. J. Grim, K. G. Jarvis, V. Sathyamoorthy, L. Hu, A. R. Datta, I. R. Patel, S. A. Jackson, J. Gangiredla, M. L. Kotewicz, J. E. LeClerc, M. Wekell, B. A. McCardell, M. D. Solomotis, B. D. Tall
Article
Food Science & Technology
Karen G. Jarvis, Qiong Q. Yan, Christopher J. Grim, Karen A. Power, Augusto A. Franco, Lan Hu, Gopal Gopinath, Venugopal Sathyamoorthy, Michael L. Kotewicz, Mahendra H. Kothary, Chloe Lee, Jennifer Sadowski, Seamus Fanning, Ben D. Tall
FOODBORNE PATHOGENS AND DISEASE
(2013)
Article
Microbiology
Roger Stephan, Christopher J. Grim, Gopal R. Gopinath, Mark K. Mammel, Venugopal Sathyamoorthy, Larisa H. Trach, Hannah R. Chase, Seamus Fanning, Ben D. Tall
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2014)
Review
Microbiology
Ziad W. Jaradat, Waseem Al Mousa, Ahmed Elbetieha, Anas Al Nabulsi, Ben D. Tall
JOURNAL OF MEDICAL MICROBIOLOGY
(2014)
Article
Multidisciplinary Sciences
Bradd J. Haley, Seon Young Choi, Christopher J. Grim, Tiffiani J. Onifade, Hediye N. Cinar, Ben D. Tall, Elisa Taviani, Nur A. Hasan, AbdulShakur H. Abdullah, Laurenda Carter, Surasri N. Sahu, Mahendra H. Kothary, Arlene Chen, Ron Baker, Richard Hutchinson, Carina Blackmore, Thomas A. Cebula, Anwar Huq, Rita R. Colwell
Review
Food Science & Technology
Rajni Chauhan, Ben D. Tall, Gopal Gopinath, Wamik Azmi, Gunjan Goel
Summary: This article reviews the factors and strategies responsible for the survival and persistence of Cronobacter sakazakii in powdered infant formula (PIF), focusing on thermal resistance, antibiotic or antimicrobial resistance, virulence potential, and stress-associated gene expression. This pathogen poses a high risk to neonates and its ability to survive in low-moisture foods is influenced by intrinsic and extrinsic factors.
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION
(2022)
Article
Microbiology
Laurenda Carter, Mei-Chiung J. Huang, Kyuyoung Han, Jayanthi Gangiredla, Jenny Yee, Hannah R. Chase, Flavia Negrete, Ben D. Tall
Summary: In this study, Bacillus cereus strains isolated from imported baby wipes were characterized using a comprehensive molecular approach. The analysis of enterotoxigenic potential, genomics, and carbohydrate utilization provided insights into the phylogenetic relatedness of these strains and their potential infection risk to vulnerable infants.
Article
Biotechnology & Applied Microbiology
Hisham Abdelrahman, Mohamed ElHady, Acacia Alcivar-Warren, Standish Allen, Rafet Al-Tobasei, Lisui Bao, Ben Beck, Harvey Blackburn, Brian Bosworth, John Buchanan, Jesse Chappell, William Daniels, Sheng Dong, Rex Dunham, Evan Durland, Ahmed Elaswad, Marta Gomez-Chiarri, Kamal Gosh, Ximing Guo, Perry Hackett, Terry Hanson, Dennis Hedgecock, Tiffany Howard, Leigh Holland, Molly Jackson, Yulin Jin, Karim Kahlil, Thomas Kocher, Tim Leeds, Ning Li, Lauren Lindsey, Shikai Liu, Zhanjiang Liu, Kyle Martin, Romi Novriadi, Ramjie Odin, Yniv Palti, Eric Peatman, Dina Proestou, Guyu Qin, Benjamin Reading, Caird Rexroad, Steven Roberts, Mohamed Salem, Andrew Severin, Huitong Shi, Craig Shoemaker, Sheila Stiles, Suxu Tan, Kathy F. J. Tang, Wilawan Thongda, Terrence Tiersch, Joseph Tomasso, Wendy Tri Prabowo, Roger Vallejo, Hein van der Steen, Khoi Vo, Geoff Waldbieser, Hanping Wang, Xiaozhu Wang, Jianhai Xiang, Yujia Yang, Roger Yant, Zihao Yuan, Qifan Zeng, Tao Zhou
