Article
Veterinary Sciences
Xiaozhou Wang, Mingchao Liu, Na Geng, Yongzhen Du, Zhaoming Li, Xin Gao, Bo Han, Jianzhu Liu, Yongxia Liu
Summary: Cell death and inflammation are closely linked during mastitis caused by Staphylococcus aureus. Bovine mammary epithelial cells respond to S. aureus by NLRP3 inflammasome activation, leading to cell apoptosis and pyroptosis. This cell death process is associated with caspase-1 activation.
VETERINARY RESEARCH
(2022)
Article
Microbiology
Dianwen Xu, Guiqiu Hu, Jianchun Luo, Ji Cheng, Di Wu, Lisha Cheng, Xuejie Huang, Shoupeng Fu, Juxiong Liu
Summary: This study investigates the role and mechanism of mitophagy in the intracellular survival of Staphylococcus aureus (S.aureus) within bovine mammary epithelial cells (BMECs). The findings showed that S.aureus induced complete mitophagy to promote its survival within BMECs, which was mediated by the activation of the p38-PINK1-Parkin signaling pathway.
VETERINARY MICROBIOLOGY
(2023)
Article
Immunology
Haojun Xu, Changjie Lin, Ting Li, Yifan Zhu, Jinghan Yang, Sijie Chen, Jianguo Chen, Xi Chen, Yingyu Chen, Aizhen Guo, Changmin Hu
Summary: In this study, the researchers aimed to investigate the effect of Staphylococcus aureus and Escherichia coli on m(6)A-modified circRNAs in bovine mammary epithelial cells. They found that these modified circRNAs were associated with cell injury, inflammation, apoptosis, and autophagy, and identified their regulatory roles in MAPK, WNT, and inflammation pathways.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Xiao-Yan Zhu, Meng-Ling Wang, Meng Cai, Xue-Mei Nan, Yi-Guang Zhao, Ben-Hai Xiong, Liang Yang
Summary: Mastitis is a common infectious disease in dairy farms that results in decreased milk production and quality. Staphylococcus aureus is one of the main bacteria causing subclinical mastitis. Exosomes play a role in various physiological and pathological functions by containing proteins, lipids, and nucleic acids.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Biology
Mara D. Saenz-de-Juano, Giulia Silvestrelli, Andres Weber, Christian Rohrig, Mathias Schmelcher, Susanne E. Ulbrich
Summary: This study investigates the impact of Staphylococcus aureus extracellular vesicles on mammary epithelial cells, providing insights into how the bacteria evades host defense mechanisms and colonizes the mammary gland effectively.
Article
Biochemistry & Molecular Biology
Changjie Lin, Yifan Zhu, Zhiyu Hao, Haojun Xu, Ting Li, Jinghan Yang, Xi Chen, Yingyu Chen, Aizhen Guo, Changmin Hu
Summary: This study investigated the effects of E.coli and S.aureus on bovine mammary epithelial cell injuries and explored the lncRNA profile. The results showed differential expression of a large number of lncRNAs induced by these two pathogens, targeting signaling pathways with similar functions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Veterinary Sciences
Bruno Toledo-Silva, Ana Claudia Dumont Oliveira, Fernando N. Souza, Freddy Haesebrouck, Sarne De Vliegher
Summary: This study found that cell-free supernatants from bovine non-aureus staphylococcal isolates can prevent adhesion and internalization of Staphylococcus aureus into bovine mammary epithelial cells, potentially through the S. aureus accessory gene regulator system. These findings provide new anti-virulence strategies for treating and controlling bovine S. aureus mastitis.
VETERINARY RESEARCH
(2023)
Article
Microbiology
Marco Antonio Barajas-Mendiola, Maria Guadalupe Salgado-Lora, Joel Edmundo Lopez-Meza, Alejandra Ochoa-Zarzosa
Summary: This study investigates the epigenetic regulation of bovine mammary epithelial cells (bMECs) challenged with Staphylococcus aureus induced by bovine prolactin (bPRL). The results show that bPRL alters specific epigenetic marks, affects the expression of inflammatory genes, and regulates the expression of miRNAs, allowing S. aureus to persist and evade the host immune response.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Kangjun Liu, Xi Zhou, Li Fang, Junsheng Dong, Luying Cui, Jun Li, Xia Meng, Guoqiang Zhu, Jianji Li, Heng Wang
Summary: Staphylococcus aureus (S. aureus) has the ability to invade mammalian cells and protect itself from clearance by the immune system. The role of mitophagy in S. aureus-associated bovine mastitis has been unclear. This study found that S. aureus infection induces mitophagy and enhances inflammation, contributing to S. aureus survival.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Biology
Shamsaldeen Ibrahim Saeed, Erkihun Aklilu, Khalid M. Mohammedsalih, Adewole A. Adekola, Ahmed Elmontaser Mergani, Maizan Mohamad, Nor Fadhilah Kamaruzzaman
Summary: The study evaluated the potential antibacterial properties of ikarugamycin as a novel antibiotic candidate against intracellular persistent infection caused by Staphylococcus aureus, highlighting its intracellular killing activity and potential application in treating S. aureus mastitis in the future.
Article
Veterinary Sciences
Yuhao Chen, Yuze Ma, Qiang Ji, Xiaoru Yang, Xue Feng, Ruiyuan Yao, Xiaoou Cheng, Tingting Li, Yanfeng Wang, Zhigang Wang
Summary: The study revealed that S. aureus infection decreases milk protein synthesis in bovine mammary epithelial cells, reducing amino acid uptake, impairing mTORC1 signaling, and inhibiting the activity of NF-kappa B and STAT5.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Microbiology
Yi-Tian Ying, Wei-Jia Ren, Xun Tan, Jing Yang, Rui Liu, Ai-Fang Du
Summary: The membrane-binding protein Annexin A2 plays a role in the internalization of Staphylococcus aureus into bovine mammary epithelial cells, with the interaction between ClfB and AnxA2 promoting the invasion of S. aureus. Targeting this interaction may provide protection against S. aureus mastitis.
Article
Cell Biology
Yi-Tian Ying, Jing Yang, Xun Tan, Rui Liu, Ying Zhuang, Jia-Xue Xu, Wei-Jia Ren
Summary: Escherichia coli and Staphylococcus aureus induce distinct immune and inflammatory responses in dairy cattle mastitis, with Nrf2 pathway playing a key role in this difference. Activation of Nrf2 is essential for bovine mammary epithelial cells to mount an effective innate defensive response, while Staphylococcus aureus impairs Nrf2 activation leading to a weaker inflammatory response.
Article
Biochemistry & Molecular Biology
Ruiyuan Yao, Manshulin Wang, Yue Zhao, Qiang Ji, Xue Feng, Linfeng Bai, Lili Bao, Yanfeng Wang, Huifang Hao, Xihe Li, Zhigang Wang
Summary: This study reveals the regulation of lipid synthesis under Staphylococcus aureus infection through mTORC1 signaling and identifies the protective effects of CGA on lipid synthesis in bovine mammary epithelial cells.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS
(2023)
Article
Immunology
Bo Liu, Qianru Li, Zhiguo Gong, Jiamin Zhao, Baichen Gu, Shuang Feng
Summary: This study found that lipoproteins from Staphylococcus aureus play a crucial role in inducing inflammatory responses and mediating bacterial internalization into bovine mammary epithelial cells.
MICROBIAL PATHOGENESIS
(2022)
Correction
Multidisciplinary Sciences
Ichiro Imanishi, Aurelie Nicolas, Ana-Carolina Barbosa Caetano, Thiago Luiz de Paula Castro, Natayme Rocha Tartaglia, Ricardo Mariutti, Eric Guedon, Sergine Even, Nadia Berkova, Raghuvir K. Arni, Nubia Seyffert, Vasco Azevedo, Koji Nishifuji, Yves Le Loir
SCIENTIFIC REPORTS
(2020)
Article
Biotechnology & Applied Microbiology
Vinicius de Rezende Rodovalho, Brenda Silva Rosa da Luz, Aurelie Nicolas, Fillipe Luiz Rosa do Carmo, Julien Jardin, Valerie Briard-Bion, Gwenael Jan, Yves Le Loir, Vasco Ariston de Carvalho Azevedo, Eric Guedon
Summary: Propionibacterium freudenreichii, a probiotic Gram-positive bacterium, produces extracellular vesicles (EVs) with immunomodulatory properties. The protein content and anti-inflammatory activity of EVs vary depending on the culture medium used, suggesting the potential for modulating therapeutic applications through growth conditions.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Microbiology
F. Blanchet, L. Rault, V Peton, Y. Le Loir, C. Blondeau, L. Lenoir, M. Dubourdeaux, S. Even
Summary: The study evaluated the potential of live and heat-inactivated Lactobacillus gasseri LA806 in combating Staphylococcus aureus and Escherichia coli infections. Live LA806 significantly reduced S. aureus adhesion and internalization, as well as pro-inflammatory cytokine expression, while inactivated LA806 showed lower antibacterial ability but minimal impact on anti-inflammatory properties.
BENEFICIAL MICROBES
(2021)
Article
Microbiology
Lucie Rault, Pierre-Alexandre Leveque, Sarah Barbey, Frederic Launay, Helene Larroque, Yves Le Loir, Pierre Germon, Jocelyne Guinard-Flament, Sergine Even
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Sabine Leroy, Sergine Even, Pierre Micheau, Anne de la Foye, Valerie Laroute, Yves Le Loir, Regine Talon
Article
Agriculture, Dairy & Animal Science
X. Yu, N. Leconte, S. Mejean, G. Garric, S. Even, G. Henry, F. J. Tessier, M. Howsam, T. Croguennec, G. Gesan-Guiziou, D. Dupont, R. Jeantet, A. Deglaire
Summary: The study aimed to investigate innovative minimal processing routes for the production of first-age infant formula powder while ensuring microbial safety with minimal protein denaturation. Three IF powders were produced using different heat treatments, with the powder produced without heat treatment showing the lowest protein denaturation extent. This study demonstrates the feasibility of producing bacteriologically safe infant formula with a high content of native proteins at a semi-industrial scale.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Food Science & Technology
Fanny Guyomarc'h, Gaelle Arvisenet, Said Bouhallab, Fanny Canon, Stephanie-Marie Deutsch, Valentin Drigon, Didier Dupont, Marie-Helene Famelart, Gilles Garric, Eric Gueden, Thibaut Guyot, Manon Hiolle, Gwenael Jan, Yves Le Loir, Valerie Lechevalier, Francoise Nau, Stephane Pezennec, Anne Thierry, Florence Valence, Valerie Gagnaire
Summary: The review focuses on innovative mixes of animal and plant ingredients, especially legumes, to create products like dairy or egg gels, emulsions, or foams. These products offer enhanced protein stability and synergistic properties, potentially serving as a flexible tool for food design. Fermentation can further enhance the nutritional and sensory aspects of these mixes, making them a possible solution to reduce animal protein consumption while maintaining essential nutrients and sensory qualities.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Microbiology
Brenda Silva Rosa Da Luz, Aurelie Nicolas, Svetlana Chabelskaya, Vinicius de Rezende Rodovalho, Yves Le Loir, Vasco Ariston de Carvalho Azevedo, Brice Felden, Eric Guedon
Summary: The study delved into the RNA content of Staphylococcus aureus EVs, revealing a diverse range of RNAs including those related to virulence and ribosomal proteins. The composition of EV RNAs was found to be significantly influenced by growth phase and environmental factors.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Barbara F. Cordeiro, Juliana L. Alves, Giovanna A. Belo, Emiliano R. Oliveira, Marina P. Braga, Sara H. da Silva, Luisa Lemos, Jonas T. Guimaraes, Ramon Silva, Ramon S. Rocha, Gwenael Jan, Yves Le Loir, Marcia Cristina Silva, Monica Q. Freitas, Erick A. Esmerino, Alfonso Gala-Garcia, Enio Ferreira, Ana Maria C. Faria, Adriano G. Cruz, Vasco Azevedo, Fillipe L. R. do Carmo
Summary: The study demonstrated that probiotic Minas Frescal cheese containing Lactococcus lactis NCDO 2118 can prevent DSS-induced colitis in mice. Consumption of probiotic cheese reduced the severity of colitis, alleviating weight loss, disease activity index, colon shortening, and histopathological score. Additionally, probiotic cheese increased the expression of tight junctions' proteins and IL-10 release, suggesting its potential as a new probiotic functional food for personalized nutrition in the context of IBD.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Nutrition & Dietetics
Gaelle Boudry, Elise Charton, Isabell Le Huerou-Luron, Stephanie Ferret-Bernard, Sophie Le Gall, Sergine Even, Sophie Blat
Summary: The assembly of gut microbiota in newborns during the first months of life is a coordinated process influenced by early bacterial colonization, environmental factors, and maternal diet. Breastfeeding plays a crucial role in shaping a healthy microbiota in infants, with human milk composition impacting the establishment of gut microbiota. Understanding these mechanisms can provide opportunities for programming health through microbiota modulation in early life.
FRONTIERS IN NUTRITION
(2021)
Article
Pharmacology & Pharmacy
Bruna Savassi, Barbara F. Cordeiro, Sara H. Silva, Emiliano R. Oliveira, Giovanna Belo, Alessandra Gomes Figueiroa, Maria Izabel Alves Queiroz, Ana Maria Caetano Faria, Juliana Alves, Tales Fernando da Silva, Gabriela Munis Campos, Erick A. Esmerino, Ramon S. Rocha, Monica Q. Freitas, Marcia C. Silva, Adriano G. Cruz, Katia Duarte Vital, Simone O. A. Fernandes, Valbert N. Cardoso, Leonardo Borges Acurcio, Gwenael Jan, Yves Le Loir, Alfonso Gala-Garcia, Fillipe Luiz R. do Carmo, Vasco Azevedo
Summary: The symbiotic product developed in this study shows significant improvement in symptoms of mucositis induced by 5-FU, including reducing weight loss and intestinal permeability, lowering inflammation score, and decreasing levels of pro-inflammatory cytokines.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Pharmacology & Pharmacy
Giovanna A. Belo, Barbara F. Cordeiro, Emiliano R. Oliveira, Marina P. Braga, Sara H. da Silva, Bruno G. Costa, Flaviano dos S. Martins, Gwenael Jan, Yves Le Loir, Alfonso Gala-Garcia, Enio Ferreira, Vasco Azevedo, Fillipe L. R. do Carmo
Summary: The study showed that the SlpB protein can enhance the probiotic potential of L. lactis strain to alleviate DSS-induced colitis in mice.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Emeline Roux, Aurelie Nicolas, Florence Valence, Gregoire Siekaniec, Victoria Chuat, Jacques Nicolas, Yves Le Loir, Eric Guedon
Summary: The study analyzed various health-related functions at the genome level of Streptococcus thermophilus strains isolated from diverse products and locations. While some functions are widely conserved among isolates, others such as the tagatose-6-phosphate pathway and the production of bioactive peptides and gamma-aminobutyric acid are strain-specific. The study also investigated the genetic basis for phenotypic diversity in certain health-related traits among strains.
Article
Microbiology
Brenda Silva Rosa da Luz, Vinicius de Rezende Rodovalho, Aurelie Nicolas, Svetlana Chabelskaya, Julien Jardin, Valerie Briard-Bion, Yves Le Loir, Vasco Ariston de Carvalho Azevedo, Eric Guedon
Summary: This study provides a comprehensive characterization of the protein composition of Staphylococcus aureus extracellular vesicles (EVs) under different growth conditions. The results show that the EV protein composition is modulated by the environment and differs from that of whole cells, with a more pronounced impact of the growth conditions on EVs compared to whole cells. This study paves the way for future research on the production, composition, and functions of S. aureus EVs.