Article
Microbiology
Yusuke Ota, Isaac Prah, Samiratu Mahazu, Yoshiaki Gu, Yoko Nukui, Ryuji Koike, Ryoichi Saito
Summary: In this study, bla(GES)-encoding plasmids were characterized from a single-hospital sewage sample in Japan. The results showed that bla(GES) genes were mainly located on different types of plasmids, which were closely related to plasmids from Japan, the United States, and Europe. The bla(GES) genes were located on the class 1 integron cassette of the Tn3 transposon-related region. These findings provide insights into the diversity and spread of resistance and highlight the importance of continuous monitoring of environmental GES-type carbapenemase producers.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Julian Sommer, Kristina M. Gerbracht, Felix F. Krause, Florian Wild, Manuela Tietgen, Sara Riedel-Christ, Janko Sattler, Axel Hamprecht, Volkhard A. J. Kempf, Stephan Goettig
Summary: The study identified OXA-484 as a carbapenemase with high sequence similarity plasmids, showing low frequency in horizontal gene transfer. OXA-484 exhibits antibiotic activity between OXA-232 and OXA-244, demonstrating unique resistance characteristics.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Evolutionary Biology
Linyue Zhang, Ying Fu, Linghong Zhang, Qingye Xu, Yunxing Yang, Jintao He, Sebastian Leptihn, Belinda Loh, Robert A. Moran, Willem van Schaik, Mark Alexander Toleman, Qiong Chen, Lilin Liu, Yunsong Yu, Xiaoting Hua
Summary: This study investigates the co-evolutionary dynamics between carbapenem-resistant Acinetobacter baumannii and a plasmid encoding OXA-23 carbapenemase. The researchers found that the bacteria gradually adapted to the presence of the antibiotic imipenem through mechanisms such as duplication of the OXA-23 gene and chromosomal adaptations, leading to improved fitness of plasmid-carrying cells.
EVOLUTIONARY APPLICATIONS
(2022)
Article
Environmental Sciences
Di Wang, Bjorn Berglund, Qi Li, Xiaorong Shangguan, Jingjing Li, Feng Liu, Fanghui Yao, Xuewen Li
Summary: Carbapenem-resistant Enterobacterales (CRE) pose an urgent threat to global public health and their transmission is facilitated through the environment. Wastewater treatment plants (WWTPs) are considered important sources of antibiotic resistance and hotspots of antibiotic-resistant bacteria (ARB), thereby promoting the dissemination of antibiotic resistance genes. This study investigated the occurrence and characteristics of carbapenem-resistant Escherichia coli (CREC) in a WWTP in Jinan, Shandong province, China. The detection rate of CREC in the WWTP water inlet was found to be 85%, and all CREC isolates were multidrug-resistant.
ENVIRONMENTAL POLLUTION
(2023)
Article
Engineering, Environmental
Octavio Alves, Luis Calado, Roberta M. Panizio, Margarida Goncalves, Eliseu Monteiro, Paulo Brito
Summary: Gasification of waste is a promising solution with better environmental impact, but requires techno-economic studies to determine feasibility and optimize costs. A study on a small-scale gasification plant processing solid recovered fuels and sewage sludge found that both electricity production and hydrogen production scenarios were economically viable, with capital expenditures having the most significant influence on economic indicators.
Article
Environmental Sciences
Pamela Maria Oliveira, Celio Faria-Junior, Daniely Martins Silva, Larissa Fernandes Matos, Alex Leite Pereira
Summary: This study investigated carbapenem-resistant Klebsiella pneumoniae (CR-Kp) strains isolated from sewage treatment plants (STPs) and their resistance mechanisms. It was found that sewage contained clinically relevant clones of CR-Kp, which resisted sewage treatments and subsequently contaminated downstream water bodies. The occurrence of resistant strains was positively associated with the number of hospitalized patients in the areas serviced by STPs.
JOURNAL OF WATER AND HEALTH
(2023)
Article
Environmental Sciences
Folake Temitope Fadare, Anthony Ifeanyi Okoh
Summary: The effluents of wastewater treatment plants in the Eastern Cape Province of South Africa contain a high abundance of multidrug-resistant Enterobacteriaceae, indicating a high antibiotic pressure environment. This highlights the need to optimize current WWTP processes to reduce potential risks of environmental contamination and health hazards.
FRONTIERS IN ENVIRONMENTAL SCIENCE
(2021)
Article
Medicine, General & Internal
Ji-Na Gu, Lin Chen, Xing-Bei Weng, Xiao-Yan Yang, Dan-Mei Pan
Summary: The research revealed that Escherichia coli Huamei202001 belongs to sequence type 410 and carries a blaNDM-5-encoding IncX3-type plasmid responsible for carbapenem resistance spreading. Only the patient was detected as having a positive faecal sample screening test for CRE.
FRONTIERS IN MEDICINE
(2021)
Article
Agricultural Engineering
Xiao-Hong Chen, Yu-Hong Huang, Huixiong Lu, Ce-Hui Mo, Lei Xiang, Nai-Xiang Feng, Hai-Ming Zhao, Hui Li, Yan-Wen Li, Quan-Ying Cai
Summary: The study found that plant-scale hyperthermophilic composting effectively reduces the concentration of organic pollutants and alters the structure and function of bacterial communities. Microbes capable of degrading pollutants were found in the compost, and hyperthermophilic composting promotes the dissipation and humification of organic pollutants.
BIORESOURCE TECHNOLOGY
(2022)
Article
Microbiology
Abimbola Olumide Adekanmbi, Olabisi Comfort Akinlabi, Adedolapo Victoria Olaposi
Summary: There is a rapid rise in the incidence of quinolone resistant bacteria in Nigeria. Most studies in Nigeria have focused on isolates from the clinical settings, with few focusing on isolates of environmental origin. This study aimed to investigate the antibiogram and carriage of plasmid-mediated quinolone resistance (PMQR) genes by quinolone-resistant isolates obtained from a pool of cefotaxime-resistant Escherichia coli (E. coli) recovered from sewage leaking out of some surface-leaking sanitary sewers in a University community in Nigeria. The findings showed a high level of resistance to antibiotics and carriage of PMQR genes by quinolone-resistant E. coli obtained from the leaking sanitary sewers, suggesting a potential environmental and public health concern.
ARCHIVES OF MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Di Wang, Huiyun Zou, Ling Zhao, Qi Li, Min Meng, Xuewen Li, Bjorn Berglund
Summary: This study investigated the occurrence and characteristics of carbapenem and colistin-resistant Escherichia coli (CCREC) in wastewater and sludge samples from a urban wastewater treatment plant in Jinan, China. The study found that 8 out of 168 samples tested positive for CCREC, with high prevalence of multidrug resistance and the presence of virulence genes. The study also observed that the resistance genes could be transferred through conjugation, indicating the potential for further dissemination of resistant E. coli.
INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH
(2023)
Article
Green & Sustainable Science & Technology
Min-Sang Kim, Hongmok Jo, Yeongmi Park, Uijeong Han, Ajay Thapa, Kyunghyun Kim, Du Hyeong Choi, Gwang Jo Park, Si-Kyung Cho
Summary: A novel pilot-scale wet electrostatic precipitator (WESP) was developed and installed in a bio-drying-assisted solid recovered fuel (SRF) generation plant for the first time, demonstrating promising performances in achieving high collection efficiencies of particulate matter. The experimental findings provide useful information to understand the WESP system, despite observing a significant increase in certain particle matter sizes.
Article
Medical Laboratory Technology
Fatma Kalayci-Yuksek, Defne Gumus, Aysun Uyanik-Ocal, Gokhan Gun, Derya Bayirli-Turan, Asli-Ceren Macunluoglu, Yasar Nakipoglu, Mine Ang-Kucuker
Summary: This study investigated the prevalence of antibiotic resistance in multidrug-resistant Klebsiella strains isolated from hospitals. The study found a high prevalence of carbapenemase genes, mobile colistin-resistance genes, and integrons in these strains. The findings highlight the importance of surveillance on emerging antibiotic resistance.
CLINICAL LABORATORY
(2023)
Article
Medical Laboratory Technology
Fatma Kalayci-Yuksek, Defne Gumus, Aysun Uyanik-Ocal, Gokhan Gun, Derya Bayirli-Turan, Asli-Ceren Macunluoglu, Yasar Nakipoglu, Mine Ang-Kucuker
Summary: This study reports the first human isolate from Turkey with co-presence of blaNDM, blaOXA-48, and both Class I and Class II integrons. The findings highlight the dissemination of integrons and carbapenemases, and emphasize the importance of surveillance on emerging antibiotic resistance.
CLINICAL LABORATORY
(2022)
Article
Biotechnology & Applied Microbiology
Jie Lian, Georg Steinert, Jeroen de Vree, Sven Meijer, Christa Heryanto, Rouke Bosma, Rene H. Wijffels, Maria J. Barbosa, Hauke Smidt, Detmer Sipkema
Summary: This study investigated the bacterial diversity in indoor and outdoor algae reactors, and found significant differences in bacterial community composition between different reactors and over time. The presence of certain bacterial taxa was significantly correlated with nitrate concentration.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Infectious Diseases
Remy A. Bonnin, Sandrine Bernabeu, Cecile Emeraud, Thierry Naas, Delphine Girlich, Agnes B. Jousset, Laurent Dortet
Summary: This study demonstrated the high in vitro efficacy of ceftazidime/avibactam, imipenem/relebactam, and to a lesser extent meropenem/vaborbactam against carbapenem-resistant non-carbapenemase-producing Enterobacterales. Testing all beta-lactam/beta-lactamase inhibitor combinations is crucial for carbapenem-resistant Enterobacterales (CRE) as resistance to one combination does not predict resistance to another, depending on the resistance mechanisms involved.
Article
Microbiology
Otavio Hallal Ferreira Raro, Patrice Nordmann, Manuel Dominguez Pino, Jacqueline Findlay, Laurent Poirel
Summary: There is an increasing occurrence of multidrug-resistant (MDR) and hypervirulent (hv) Klebsiella pneumoniae (MDR-hvKp) convergent clones. These strains can cause difficult-to-treat infections and have a higher mortality rate in healthy adults. This study in Switzerland identified a rate of 9.0% K. pneumoniae with a virulence genotype, producing carbapenemase and recovered from rectal swabs, urine, and blood. The presence of alarming MDR-hvKp and MDR nonhypervirulent K. pneumoniae (MDR-nhv-Kp) isolates highlights the need for surveillance networks to track and monitor their spread.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Jacqueline Findlay, Laurent Poirel, Maxime Bouvier, Valeria Gaia, Patrice Nordmann
Summary: Carbapenem-resistant Enterobacterales, including KPC-producing Klebsiella pneumoniae, pose a major threat to public health due to their rapid spread. Ceftazidime-avibactam, a beta-lactam/beta-lactamase inhibitor combination, has shown excellent activity against multidrug-resistant KPC-producing Enterobacterales strains. However, there is an increasing occurrence of CAZ-AVI-resistant K. pneumoniae isolates, mainly due to the emergence of KPC variants that confer resistance to CAZ-AVI but retain carbapenem resistance.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Christophe Le Terrier, Patrice Nordmann, Mustafa Sadek, Laurent Poirel
Summary: This study evaluated the in vitro activity of cefepime/taniborbactam and cefepime/zidebactam against aztreonam/avibactam-resistant E. coli strains. The results showed that cefepime/taniborbactam had a resistance rate of 100% against these strains, while cefepime/zidebactam was effective in all cases. This suggests that cefepime/zidebactam combination could be a promising therapeutic option.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Ahmad Ibrahim, Maxime Bouvier, Mustafa Sadek, Jean-Winoc Decousser, Laurent Poirel, Patrice Nordmann
Summary: Cefiderocol (FDC) is an effective siderophore cephalosporin against multidrug-resistant Gram-negative bacteria. The SuperFDC medium was developed to rapidly and accurately identify FDC-resistant pathogens, which is crucial for controlling their spread. The medium showed high sensitivity and specificity in detecting FDC-resistant isolates with various resistance mechanisms.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Editorial Material
Infectious Diseases
Delphine Girlich, Cecile Emeraud, Oceane Vanparis, Thierry Naas, Remy A. Bonnin, Laurent Dortet
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2023)
Article
Infectious Diseases
Otavio Hallal Ferreira Raro, Maxime Bouvier, Auriane Kerbol, Jean-Winoc Decousser, Laurent Poirel, Patrice Nordmann
Summary: The study developed a rapid test for the detection of Acinetobacter baumannii susceptibility to the antibiotic cefiderocol. The test showed high sensitivity and specificity and significantly reduced the time required compared to current methods.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Patrice Nordmann, Maxime Bouvier, Laurent Poirel
Summary: Carbapenemase-producing Enterobacterales (CPE) are a major health concern globally, with limited therapeutic options in Switzerland. This study evaluated the susceptibility of CPE isolates recovered in Switzerland from 2018 to 2020 to novel β-lactam/β-lactamase inhibitor combinations, including ceftazidime-avibactam, meropenem-vaborbactam, and imipenem-relebactam. The majority of clinical CPE isolates were Klebsiella pneumoniae and Escherichia coli, and the distribution of carbapenemases varied. The study found that meropenem-vaborbactam had the highest susceptibility among the three combinations, followed by ceftazidime-avibactam and imipenem-relebactam. These findings can help optimize first-line therapy for CPE infections.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Christophe Le Terrier, Patrice Nordmann, Chloe Buchs, Doris Yoong Wen Di, Gian Maria Rossolini, Roger Stephan, Mariana Castanheira, Laurent Poirel
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Otavio Hallal Ferreira Raro, Laurent Poirel, Maurine Tocco, Patrice Nordmann
Summary: The study aimed to evaluate whether subdosages of antibiotics administered in veterinary medicine could enhance plasmid transfer and resistance gene exchange in gut microbiota. The results showed that antibiotics in veterinary medicine may induce the transfer of plasmid-encoded resistance and contribute to the global spread of antibiotic resistance genes.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Editorial Material
Infectious Diseases
Jacqueline Findlay, Laurent Poirel, Patrice Nordmann
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Clement Viguier, Maxime Bouvier, Mustafa Sadek, Auriane Kerbol, Laurent Poirel, Patrice Nordmann
Summary: This study develops a rapid culture-based test for the identification of Aztreonam-avibactam resistance among multidrug-resistant Enterobacterales. The test is rapid, highly sensitive, specific, easily interpretable, and can be easily implemented in routine laboratory practices.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Microbiology
Julia Hassa, Tom Jonas Tubbesing, Irena Maus, Robert Heyer, Dirk Benndorf, Mathias Effenberger, Christian Henke, Benedikt Osterholz, Michael Beckstette, Alfred Puehler, Alexander Sczyrba, Andreas Schlueter
Summary: This study used metagenomic and metaproteomic analyses to investigate the microbial communities in a full-scale biogas plant with three differently operated digesters. The results showed that these microbial communities had a significant impact on the abundance of enzymes and genes involved in the biogas process, and identified key microorganisms involved in different steps of anaerobic digestion. Further analysis of the highly abundant MAGs in all digesters indicated their potential in ensuring biogas process stability.
