Article
Microbiology
Yunfei Hu, Chunfang Yang, Can Song, Weixuan Zhong, Baiyuan Li, Linyan Cao, Huahai Chen, Changhui Zhao, Yeshi Yin
Summary: A new DZNR from Clostridium sp. ZJ6 was discovered, which has bidirectional enzyme activities and is widely distributed in the gut of humans and animals.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Bing-Juan Li, Meng-Ying Xiao, Xin-Yu Dong, Zhao-Xiang Huang
Summary: The study describes the improvement of engineered E. coli for the production of (S)-equol, the most active metabolite of soybean isoflavones. The optimal conditions for (S)-equol production were explored, leading to high yields of (S)-equol.
FOOD SCIENCE & NUTRITION
(2022)
Article
Chemistry, Organic
Takefumi Kuranaga, Miho Tamura, Hiroaki Ikeda, Sakahiro Terada, Yusuke Nakagawa, Hideaki Kakeya
Summary: The study detected presaccharothriolide Z and achieved its chemical synthesis, providing the first total synthesis of saccharothriolide-class macrolides and determining its structure and biological activity.
Article
Chemistry, Multidisciplinary
Xiaoya Gao, Jianxin Tian, Shujin Cheng, Zicheng Zuo, Rui Wen, Feng He, Yuliang Li
Summary: A multi-level architecture consisting of conformal graphdiyne (GDY) and CuS is designed for Li-free cathode, which efficiently integrates the advantages of GDY and forms new functional heterojunctions. This architecture successfully avoids structural collapse through the layer-by-layer 2D confinement effect, inhibits the shuttling of active components through selective transport, and significantly regulates the phase conversion reaction through the interfacial sp-C-S-Cu hybridization bond. The GDY-based interface strategy greatly improves the reaction dynamics and reversibility, enabling the cathode to deliver a high energy density of 934 Wh kg(-1) and an unattenuated lifespan of 3000 cycles at 1 C.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Louay Abo Qoura, Elena Morozova, Vitalia Kulikova, Saida Karshieva, Darina Sokolova, Vasiliy Koval, Svetlana Revtovich, Tatyana Demidkina, Vadim S. Pokrovsky
Summary: The study investigates the anticancer effect of C115H MGL-Dz/propiin on various solid tumor types, both in vitro and in vivo. The results suggest that this pharmacological pair has the potential to suppress tumor growth and can be considered as a new strategy for the treatment of solid tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Microbiology
Mitsuo Sakamoto, Nao Ikeyama, Masahiro Yuki, Takumi Murakami, Hiroshi Mori, Takao Iino, Moriya Ohkuma
Summary: Two obligately anaerobic, Gram-positive, rod-shaped bacteria were isolated from fecal samples of healthy humans in Japan. 16S rRNA gene sequence analysis revealed that these strains belonged to the genus Adlercreutzia and possessed equol-producing capabilities. The strains were named Adlercreutzia hattorii sp. nov. and A. equolifaciens. Strain 8CFCBH1T was identified as a novel species, while strain 9CBH6 exhibited daidzein-converting activity.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Nutrition & Dietetics
Hiroaki Takeuchi, Yu Yoshikane, Hirotsugu Takenaka, Asako Kimura, Jahirul Md. Islam, Reimi Matsuda, Aoi Okamoto, Yusuke Hashimoto, Rie Yano, Koichi Yamaguchi, Shouichi Sato, Satoshi Ishizuka
Summary: The study found that drinking water MIU extracted from deep sea water with a hardness of 88 in Japan is beneficial for human gut health, increasing the production of three major SCFAs and sIgA. In the MIU group, there was a significant effect on butyric acid in the intestinal environment post-intervention, and a higher metabolic efficiency for daidzein-to-equol conversion compared to the control group.
Article
Multidisciplinary Sciences
Amir Ata Saei, Christian M. Beusch, Pierre Sabatier, Juan Astorga Wells, Hassan Gharibi, Zhaowei Meng, Alexey Chernobrovkin, Sergey Rodin, Katja Nareoja, Ann-Gerd Thorsell, Tobias Karlberg, Qing Cheng, Susanna L. Lundstrom, Massimiliano Gaetani, Akos Vegvari, Elias S. J. Arner, Herwig Schuler, Roman A. Zubarev
Summary: The SIESTA method is a high-throughput unbiased proteomics approach for identifying and prioritizing enzyme-modified substrate proteins based on changes in thermal stability. In proof-of-concept studies, it successfully identified known and novel substrate candidates for several enzyme systems. This method provides a potential tool for better understanding enzyme functions and could aid in drug discovery.
NATURE COMMUNICATIONS
(2021)
Article
Food Science & Technology
Jihong Wang, Lei Zhang, Peiwen Wang, Jinhui Lei, Lingli Zhong, Lei Zhan, Xianfeng Ye, Yan Huang, Xue Luo, Zhongli Cui, Zhoukun Li
Summary: In this study, a novel and efficient malto-oligosaccharide-forming enzyme, AmyCf, was identified. AmyCf showed a broad substrate specificity towards different types of starches and could convert gelatinized starch and raw starch granules into high-purity malto-oligosaccharides. This enzymatic conversion technique has potential for low-temperature starch conversion.
Article
Chemistry, Applied
Lili Song, Xuecheng Meng, Lei Yang, Zhenmin Ma, Minying Zhou, Chenliang Yu, Zuying Zhang, Weiwu Yu, Jiasheng Wu, Heqiang Lou
Summary: This study uncovers the molecular basis of nutrition conversion in Torreya grandis seeds and suggests that sucrose phosphate synthase, dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, glycerol-3-phosphate acyltransferase, and pyruvate kinase may play key roles in nutrition conversion.
Article
Plant Sciences
Huanhuan Li, Mengxue Zhang, Hongji Li, Hai Yu, Shuo Chen, Wenhui Wu, Peng Sun
Summary: A new venturicidin biosynthetic gene cluster (BGC) was discovered, along with four 20-membered macrolides. Venturicidin A and E showed high selective cytotoxicity against acute monocytic leukemia cells.
JOURNAL OF NATURAL PRODUCTS
(2021)
Article
Microbiology
Juan Hilario Cafiero, Maria Carla Martini, Mauricio Javier Lozano, Carolina Vacca, Antonio Lagares, Pablo Emiliano Tomatis, Maria Florencia Del Papa
Summary: This study characterized a novel subclass B2 MBL called BioF identified in an environmental Pseudomonas sp. strain, which functions as a potent carbapenemase with incipient activity against some cephalosporins. The BioF activity is not affected by excess zinc but is only inhibited at high metal chelator concentrations, highlighting the difficulty in suppressing B2 carbapenemase activity.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biochemical Research Methods
Sou Sugiki, Teppei Niide, Yoshihiro Toya, Hiroshi Shimizu
Summary: This study proposes a method for estimating the contribution of each amino acid residue to substrate specificity using phylogenetic analysis and logistic regression. By using amino acid sequences of enzymes with different cofactor specificities as input data, a highly accurate machine learning model was obtained. This method can be applied in protein engineering.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Automation & Control Systems
Mu-Yen Chen, Hsin-Te Wu
Summary: As intelligent transportation systems evolve, this article focuses on the fundamental components of vessels, harbors, and ship-shore information-communication technology applications. By integrating artificial intelligence and the 5G network, the research aims to ensure ship safety through information sharing and the implementation of geofencing technology. The proposed security mechanism detects malicious attacks and improves vessel safety through the use of the 5G network and sensors. The performance analysis and experimental results demonstrate the effectiveness of the network security approach and geofencing technology introduced in this article.
IEEE TRANSACTIONS ON INDUSTRIAL INFORMATICS
(2023)
Article
Environmental Sciences
Shalini Singh, Vipin Kumar, Pratishtha Gupta, Madhurya Ray, Ankur Singh
Summary: This study investigated a potential Hg-resistant bacterial strain (IITISM23) with high removal efficiency of Hg (II) through volatilization method in nutrient-rich medium. The strain showed high resistance towards Hg and other heavy metals, indicating its potential application in the decontamination of Hg from polluted soils.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Review
Microbiology
Hirokazu Tsuji, Kazunori Matsuda, Koji Nomoto
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Psychiatry
Emiko Aizawa, Hirokazu Tsuji, Takashi Asahara, Takuya Takahashi, Toshiya Teraishi, Sumiko Yoshida, Norie Koga, Kotaro Hattori, Miho Ota, Hiroshi Kunugi
FRONTIERS IN PSYCHIATRY
(2019)
Article
Microbiology
Yukitoshi Aoyagi, Ryuta Amamoto, Sungjin Park, Yusuke Honda, Kazuhito Shimamoto, Akira Kushiro, Hirokazu Tsuji, Hoshitaka Matsumoto, Kensuke Shimizu, Kouji Miyazaki, Satoshi Matsubara, Roy J. Shepherd
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Endocrinology & Metabolism
Naohide Kurita, Kazuo Yamashiro, Takuma Kuroki, Ryota Tanaka, Takao Urabe, Yuji Ueno, Nobukazu Miyamoto, Masashi Takanashi, Hideki Shimura, Toshiki Inaba, Yuichiro Yamashiro, Koji Nomoto, Satoshi Matsumoto, Takuya Takahashi, Hirokazu Tsuji, Takashi Asahara, Nobutaka Hattori
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2020)
Article
Microbiology
T. Shima, R. Amamoto, C. Kaga, Y. Kado, T. Sasai, O. Watanabe, J. Shiinoki, K. Iwazaki, H. Shigemura, H. Tsuji
BENEFICIAL MICROBES
(2019)
Article
Nutrition & Dietetics
Takuya Sugimoto, Tatsuichiro Shima, Ryuta Amamoto, Chiaki Kaga, Yukiko Kado, Osamu Watanabe, Junko Shiinoki, Kaoru Iwazaki, Hiroko Shigemura, Hirokazu Tsuji, Satoshi Matsumoto
Article
Biotechnology & Applied Microbiology
Yuki Saito, Akira Shigehisa, Yohei Watanabe, Naoki Tsukuda, Kaoru Moriyama-Ohara, Taeko Hara, Satoshi Matsumoto, Hirokazu Tsuji, Takahiro Matsuki
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2020)
Article
Psychology, Biological
Noriyuki Miyata, Tomokazu Hata, Shu Takakura, Kazufumi Yoshihara, Chihiro Morita, Katsunaka Mikami, Koji Nomoto, Kouji Miyazaki, Hirokazu Tsuji, Nobuyuki Sudo
Summary: The study compared the serum metabolic profiles of female patients with restricting type anorexia nervosa (ANR) to healthy female controls, revealing lower amino acid levels and higher uremic toxins levels in the patients. Serum PCS levels were positively correlated with specific gut microbes in ANR patients. These findings suggest a potential role of gut microbes in the increased uremic toxins levels in ANR patients.
PHYSIOLOGY & BEHAVIOR
(2021)
Article
Ecology
Naoki Tsukuda, Kana Yahagi, Taeko Hara, Yohei Watanabe, Hoshitaka Matsumoto, Hiroshi Mori, Koichi Higashi, Hirokazu Tsuji, Satoshi Matsumoto, Ken Kurokawa, Takahiro Matsuki
Summary: This study explored the early life relationships between SCFA and gut microbiota by analyzing faecal samples collected from 12 subjects over the first two years of life. The research identified three distinct phases of SCFA profiles and their associations with specific gut microbiota. The results showed that butyrate production was linked to Clostridiales species and breastfeeding cessation, while formate production was associated with infant-type bifidobacterial species and HMO-derived fucose.
Article
Multidisciplinary Sciences
Ryuta Amamoto, Kazuhito Shimamoto, Sungjin Park, Hoshitaka Matsumoto, Kensuke Shimizu, Miyuki Katto, Hirokazu Tsuji, Satoshi Matsubara, Roy J. Shephard, Yukitoshi Aoyagi
Summary: Approximately 10% of elderly Japanese individuals may experience significant changes in gut microbiota within a year. Habitual intake of probiotics may help stabilize gut microbiota in the elderly.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Hirotaka Shimizu, Katsuhiro Arai, Takashi Asahara, Takuya Takahashi, Hirokazu Tsuji, Satoshi Matsumoto, Ichiro Takeuchi, Reiko Kyodo, Yuichiro Yamashiro
Summary: This study found that anaerobic preparation of donor stool for fecal microbiota transplantation (FMT) can improve efficacy by enhancing the transfer of obligate anaerobes, although aerobic handling may decrease viable anaerobes in the fecal slurries. Aerobic-prep did not significantly influence the survival of facultative anaerobes.
Article
Microbiology
T. Shima, C. Kaga, K. Shimamoto, T. Sugimoto, Y. Kado, O. Watanabe, T. Suwa, R. Amamoto, H. Tsuji, S. Matsumoto
Summary: This study examined the effects of live LcS on the gut microbiome, organic acid profiles, defecatory symptoms, and serum viral antibody indexes in healthy Japanese adults. The presence of live LcS was associated with a healthy gut environment and inhibition of the reactivation of latent viruses in the host.
BENEFICIAL MICROBES
(2022)
Article
Multidisciplinary Sciences
Eiji Ishikawa, Tetsuya Yamada, Kazuaki Yamaji, Masaki Serata, Daichi Fujii, Yoshinori Umesaki, Hirokazu Tsuji, Koji Nomoto, Masahiro Ito, Nobuhiko Okada, Masato Nagaoka, Atsushi Gomi
Summary: The study revealed that a housekeeping sortase from Bifidobacterium bifidum YIT 10347 (BF-1) plays a critical role in adhesion to mucin. Multiple SDPs and pili appeared to work cooperatively in achieving adhesion, with the housekeeping sortase responsible for anchoring their substrates to the cell wall for proper biological function. Mutants lacking pilin or SDP exhibited similar adhesion properties to the wild type strain.
Article
Immunology
Mai Niikura, Satomi Atobe, Akira Takahashi, Yukiko Kado, Takuya Sugimoto, Hirokazu Tsuji, Kentaro Shimizu, Hiroshi Ogura, Takashi Asahara
Summary: A novel PA quantification system based on RT-qPCR was developed, which quickly and sensitively detected PA in clinical samples, and proved valuable in identifying infection status in ICU patients. Monitoring PA viable counts correlated with inflammatory responses of infected cells and aid in evaluating appropriate antibacterial treatment.
EMERGING MICROBES & INFECTIONS
(2021)
Article
Microbiology
Yuki Saito, Tadashi Sato, Koji Nomoto, Hirokazu Tsuji
FEMS MICROBIOLOGY ECOLOGY
(2018)