Article
Immunology
Josephine Moran, Liam Feltham, James Bagnall, Marie Goldrick, Elizabeth Lord, Catherine Nettleton, David G. Spiller, Ian Roberts, Pawel Paszek
Summary: The paper investigates the real-time interaction between the food-borne pathogen L. monocytogenes and host macrophages, revealing heterogeneous outcomes of infection and the impact of bacterial quantity on macrophage phagocytosis ability. It also highlights the role of population-level PrfA-mediated activity in regulating host-pathogen interactions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Xiaobing Jiang, Congyi Jiang, Tao Yu, Xiaojie Jiang, Siyu Ren, Rui Kang, Shuxing Qiu
Summary: BC adaptation enhances biofilm formation but reduces swarming motility in L. monocytogenes. The expression of flagella-mediated motility-related genes is downregulated in BC adapted strains. BC adaptation increases the ability of L. monocytogenes to adhere to and invade Caco-2 cells, without affecting hemolytic activity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Michelle L. Claxton, Lauren K. Hudson, Daniel W. Bryan, Thomas G. Denes
Summary: Listeria monocytogenes is a foodborne pathogen with high mortality rate. This study isolated and characterized Listeria strains from soil samples in the Great Smoky Mountains National Park, revealing a diversity of Listeria species and indicating the park's role as a reservoir. The findings provide insights into the genomic evolution and ecology of Listeria spp. and their contribution to contamination of agricultural and food processing environments.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemical Research Methods
Claudius L. Dietsche, Elisabeth Hirth, Petra S. Dittrich
Summary: A microfluidic platform was developed to quantitatively measure the secreted proteins of individual macrophages and macrophages polarized by breast cancer cells. The study successfully distinguished differently polarized macrophages by determining three signaling proteins. The results showed that macrophages treated with cancer cell supernatant exhibited a similar signaling pattern to M2 macrophages.
Article
Food Science & Technology
Divya Kode, Ramakrishna Nannapaneni, Sam Chang
Summary: Between January and July 2021, there were 30 recalls in the U.S. due to potential Listeria monocytogenes contamination in various food products. The emergence of antibiotic resistance in L. monocytogenes poses a risk to public health. Research indicates that biocides used in food processing may contribute to the development of antibiotic resistance in L. monocytogenes.
Article
Food Science & Technology
Federica D'Onofrio, Maria Schirone, Antonello Paparella, Ivanka Krasteva, Manuela Tittarelli, Francesco Pomilio, Luigi Iannetti, Nicola D'Alterio, Mirella Luciani
Summary: This study evaluated proteome profiling of Listeria monocytogenes strains grown in stress conditions using one-dimensional electrophoresis, 2D-PAGE, and tandem mass spectrometry. Proteins related to pathogenesis and stress response pathways were analyzed, and certain proteins were found only under specific stress conditions. Understanding how L. monocytogenes adapts to stress can help control its growth in food and reduce consumer risk.
Article
Food Science & Technology
Jasper W. Bannenberg, Marcel H. Tempelaars, Marcel H. Zwietering, Tjakko Abee, W. den Besten
Summary: Pathogens exhibit highly variable behavior at the single-cell level, impacting the efficacy of detection methods. Heterogeneity in single-cell growth is influenced by heat stress, selective enrichment broth, and the specific strain of Listeria monocytogenes. Low cell concentrations can impede detection due to cell heterogeneity, emphasizing the importance of considering single-cell heterogeneity in optimizing enrichment procedures for low levels of L. monocytogenes contamination.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Microbiology
Divya Kode, Ramakrishna Nannapaneni, Mohit Bansal, Sam Chang, Wen-Hsing Cheng, Chander S. Sharma, Aaron Kiess
Summary: The study found that Listeria monocytogenes developed low-level tolerance to the fluoroquinolone antibiotic ciprofloxacin after sublethal adaptation to quaternary ammonium compound (QAC), showing increased short-range MIC, growth rate, and survival in ciprofloxacin for certain strains. However, not all strains exhibited increased tolerance after QAC adaptation, suggesting the potential formation of low-level ciprofloxacin-tolerant subpopulations in certain strains when exposed to residual QAC concentrations.
Article
Food Science & Technology
Yuan Wang, Linjun Sun, Lili Hu, Zhen Wang, Xiang Wang, Qingli Dong
Summary: According to the study, the nutrition status affects the adhesion ability and biofilm formation of Listeria monocytogenes. Nutrient deficiency can enhance the adhesion and biofilm formation of the bacteria, and also lead to changes in the expression of critical biofilm-associated genes. These findings are important for understanding the spread and control of Listeria in food processing.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Jiawen Zhang, Peiying Zheng, Jing Li, Yijing Yang, Shaoxiao Zeng, Jianqing Qiu, Shaoling Lin
Summary: In this study, sono-photodynamic sterilization technology (SPDT) was used to effectively inactivate Listeria monocytogenes by inducing excessive ROS production, leading to bacterial cell deformation, membrane rupture, and triggering DNA cleavage and protein degradation.
Article
Food Science & Technology
Jialun Wu, Olivia McAuliffe, Conor P. O'Byrne
Summary: Listeria monocytogenes possesses a high proportion of carbohydrate-specific phosphotransferase system (PTS) genes in its genome, allowing it to inhabit diverse environmental niches. A study examined the carbon source utilization of L. monocytogenes strains and identified a mutation in a putative trehalose transporter gene, TreB, that affected growth and other phenotypes. Reversion mutants with restored TreB function showed improved biofilm development and acid stress resistance. These findings have important implications for food safety, as the studied strain is recommended for food challenge studies.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Benjamin J. Auerbach, Jian Hu, Muredach P. Reilly, Mingyao Li
Summary: The development of single-cell technologies has enabled the study of cellular heterogeneity, which is crucial for understanding disease molecular mechanisms. Further research requires careful computational analysis.
Article
Food Science & Technology
Fiona Zoz, Stephane Guyot, Cosette Grandvalet, Melanie Ragon, Eric Lesniewska, Sebastien Dupont, Olivier Firmesse, Brigitte Carpentier, Laurent Beney
Summary: This study investigated the impact of relative air humidity on destroying Listeria monocytogenes, finding that dehydration in PBS at 68% RH followed by rapid rehydration greatly altered cell membrane permeability and envelope, leading to only 67% recovery in cell volume. Dehydration and rehydration in city water was found to be the most efficient way to destroy cells, suggesting potential improvements in current cleaning procedures in the food industry.
Article
Biotechnology & Applied Microbiology
Duarte N. Guerreiro, Jialun Wu, Emma McDermott, Dominique Garmyn, Peter Dockery, Aoife Boyd, Pascal Piveteau, Conor P. O'Byrne
Summary: The alternative sigma factor B (sigma(B)) contributes to the stress tolerance of the foodbome pathogen Listeria monocytogenes by upregulating the general stress response. In this study, the researchers found that sigma(B) loss-of-function mutations arise frequently in strains of L. monocytogenes, and mild stresses can favor the selection of such mutations.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Cell Biology
Lalitha Tadala, Dorothee Langenbach, Mirjam Dannborg, Ramon Cervantes-Rivera, Atin Sharma, Kevin Vieth, Lisa M. Rieckmann, Alkwin Wanders, David A. Cisneros, Andrea Puhar
Summary: This study demonstrates that the mechanosensitive plasma membrane channel PIEZO1 acts as a sensor for bacterial entry and initiates immune signaling through ATP secretion and gene expression. It suggests that the detection of infection by PIEZO1 is driven by physical signals instead of chemical ligands, independent of detection of microbial molecules.
Article
Ecology
Richa Sharma, Laurent Gal, Dominique Garmyn, David Bru, Shilpi Sharma, Pascal Piveteau
Summary: This study reveals that a bacterial consortium can significantly enhance plant growth and suppress the growth of pathogens. In sterilized soil, the inoculation of bioinoculants effectively inhibits the population of Listeria monocytogenes, but in non-sterile soil, the presence of resident soil bacterial communities also plays a crucial role in this inhibitory effect.
Article
Biochemical Research Methods
Ingrid Chafsey, Rafal Ostrowski, Morgan Guilbaud, Pilar Teixeira, Jean-Marie Herry, Nelly Caccia, Christophe Chambon, Michel Hebraud, Joana Azeredo, Marie-Noelle Bellon-Fontaine, Magdalena Popowska, Mickael Desvaux
Summary: Listeria monocytogenes exhibits a dimorphism associated with SecA2 activity, with significant implications for ecophysiology and pathophysiology, affecting cell surface properties and proteosurfaceome composition.
JOURNAL OF PROTEOMICS
(2022)
Article
Multidisciplinary Sciences
Nathalie Bechon, Jovana Mihajlovic, Anne-Aurelie Lopes, Sol Vendrell-Fernandez, Julien Deschamps, Romain Briandet, Odile Sismeiro, Isabelle Martin-Verstraete, Bruno Dupuy, Jean-Marc Ghigo
Summary: This study found that bile triggers biofilm formation in B. thetaiotaomicron and other gut Bacteroidales species. Unlike many Firmicutes and Proteobacteria that rely on eDNA for biofilm formation, B. thetaiotaomicron requires BT3563-mediated eDNA degradation to form biofilms in the presence of bile.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Virgile Gueneau, Ana Rodiles, Jean-Christophe Piard, Bastien Frayssinet, Mathieu Castex, Julia Plateau-Gonthier, Romain Briandet
Summary: Little information is available about biofilm microbial communities on the surface of livestock buildings. This study proposes a reproducible coupon-based capture method and complementary ex-situ analysis tools to describe the major features of these communities. The analysis of pig farm building coupons revealed a rapid coverage of biological material and the existence of active metabolic microorganism clusters. High-throughput sequencing showed bacterial diversity profiles in accordance with reported bacteria diversity in pig intestinal ecosystems and revealed differences between materials.
Editorial Material
Microbiology
Arnaud Bridier, Romain Briandet
Summary: This article discusses the role of microbial biofilms in important ecological processes and chronic infections, as well as their relationship with the three-dimensional structure and functional properties. It also highlights the recent advances in studying biofilms using advanced technologies, and emphasizes the need for further research on cell behavior in different scales and environments.
Article
Agronomy
Catherine Henault, Elodie Barbier, Alain Hartmann, Cecile Revellin
Summary: Agriculture is a major source of greenhouse gas N2O, and this study demonstrates the potential of using specific bacterial strains to significantly reduce soil N2O emissions, not only for soybean but also for other leguminous crops and grassland.
Article
Microbiology
Virgile Gueneau, Jean-Christophe Piard, Bastien Frayssinet, Valentin Loux, Helene Chiapello, Julia Plateau-Gonthier, Mathieu Castex, Romain Briandet
Summary: The genome of Bacillus velezensis P1 strain isolated from a biofilm on a pig farm wall was sequenced, revealing the presence of numerous surface colonization genes involved in surfactant, matrix, and antibacterial synthesis.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Biotechnology & Applied Microbiology
Cedric Saint Martin, Maud Darsonval, Marina Gregoire, Nelly Caccia, Lucas Midoux, Sophie Berland, Sabine Leroy, Florence Dubois-Brissonnet, Mickael Desvaux, Romain Briandet
Summary: This study investigated the spatial patterns of Listeria monocytogenes and Escherichia coli O157:H7 colonization in gel matrices using confocal laser scanning microscopy and genetic fluorescent labeling. It was found that increasing low melting point agarose (LMPA) concentrations transitioned the bacteria from a motile single-cell lifestyle to a sessile population organized as microcolonies. The size, number, and morphology of the microcolonies were significantly affected by supplementation of NaCl or lactic acid. The presence of these compounds also partially restored single-cell motility at higher LMPA concentrations.
Review
Microbiology
Raphael Charron, Marine Boulanger, Romain Briandet, Arnaud Bridier
Summary: Bacteria in biofilms on food chain surfaces show higher tolerance to biocides used during cleaning, which raises concerns about their adaptation and resistance at the single-cell and communal levels. Research has focused on understanding the specific features of biofilms that enable bacterial populations to adapt to biocide exposure. This review discusses the diversity of adaptive strategies and the potential side effects of biofilm adaptation on antimicrobial resistance, virulence, and colonization features from a One Health perspective.
Article
Food Science & Technology
Cedric St Martin, Nelly Caccia, Maud Darsonval, Marina Gregoire, Arthur Combeau, Gregory Jubelin, Florence Dubois-Brissonnet, Sabine Leroy, Romain Briandet, Mickael Desvaux
Summary: Functional diversity within isogenic spatially organised bacterial populations can trigger emergent community properties, such as stress tolerance. This study investigated the expression of the gadB gene, which is involved in E. coli tolerance to acidic conditions, in hydrogels mimicking structured food matrices. The spatial patterns of bacterial gene expression were visualised using confocal laser scanning microscopy. The study found that the expression patterns of gadB were radically different between neutral and acidic hydrogels. Similar spatial patterns were also observed for E. coli O157:H7 grown in the presence of L. lactis. These findings have significant implications for risk assessment and public health, highlighting the need for tailored risk mitigation strategies for different food types.
NPJ SCIENCE OF FOOD
(2023)
Article
Microbiology
Yasmine Dergham, Dominique Le Coq, Arnaud Bridier, Pilar Sanchez-Vizuete, Hadi Jbara, Julien Deschamps, Kassem Hamze, Ken-ichi Yoshida, Marie-Francoise Noirot-Gros, Romain Briandet
Summary: The NDmed strain of Bacillus subtilis has the ability to form complex biofilms and protect pathogens embedded in mixed-species biofilms. It is also genetically manipulatable, making it a valuable model for studying biofilm formation in B. subtilis.
Article
Microbiology
Yicen Lin, Romain Briandet, Akos T. Kovacs
Summary: Biofilm formation is a common process among bacterial communities, allowing them to survive. Bacillus cereus is a widely distributed species, found not only in soil but also in plant roots, nematodes, and food. Understanding the mechanisms of biofilm formation is crucial for cleaning procedures in the food industry.
Article
Microbiology
Virgile Gueneau, Julia Plateau-Gonthier, Ludovic Arnaud, Jean-Christophe Piard, Mathieu Castex, Romain Briandet
Summary: The increase in human consumption of animal proteins has led to changes in meat production management, which has resulted in stricter regulations on antimicrobial products used to control pathogens. Alternative biological solutions, such as beneficial bacteria forming positive biofilms, are being developed to improve biosecurity practices in livestock buildings. However, the mechanisms and microbial composition of these new products are still not well understood.
Article
Microbiology
Pilar Sanchez-Vizuete, Yasmine Dergham, Arnaud Bridier, Julien Deschamps, Etienne Dervyn, Kassem Hamze, Stephane Aymerich, Dominique Le Coq, Romain Briandet
Summary: Bacillus subtilis can form biphasic biofilms on submerged surfaces, where bacteria initially adhere to the surface forming elongated chains before being released as free cells. The switching coincides with oxygen depletion and is followed by the formation of the biofilm at the liquid-air interface. Residual bacteria associated with the solid surface express matrix genes to build protruding structures typical of biofilms.
Review
Medicine, Research & Experimental
Celia Sahli, Sergio E. Moya, John S. Lomas, Christine Gravier-Pelletier, Romain Briandet, Miryana Hemadi
Summary: This article introduces the spatially-organized communities of microorganisms called biofilms and their interactions with antimicrobials. The limitations of antibiotic treatments are discussed, and alternative approaches using nanoparticles are presented. The aim of this article is to eradicate bacterial biofilms and fight antimicrobial tolerance using nanobiotechnology-based approaches.
