期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 78, 期 5, 页码 1611-1614出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.06693-11
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资金
- Institute of Paper Science and Technology at Georgia Tech
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0903894] Funding Source: National Science Foundation
We report here the first engineering effort for Escherichia coli biocatalysts to assimilate cellobiose through a phosphorolytic mechanism. Cytoplasmic expression of the Saccharophagus cellobiose phosphorylase was shown to enable E. coli to use cellobiose. Subsequent knockout and complementation studies provided solid evidence that the endogenous LacY was responsible for the transport of cellobiose.
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