Article
Veterinary Sciences
Pouya Reshadi, Fatemeh Heydari, Reza Ghanbarpour, Mahboube Bagheri, Maziar Jajarmi, Mohadese Amiri, Hesam Alizade, Mahdi Askari Badouei, Shademan Sahraei, Nasrin Adib
Summary: The study determined the phylogenetic background and prevalence of diarrheagenic E. coli and antimicrobial resistance in healthy riding-horses in Iran. The most prevalent antimicrobial resistance phenotypes were against amoxicillin/clavulanic acid and ceftriaxone. The study also found that 26.15% of the E. coli isolates were multi-drug resistant (MDR).
BMC VETERINARY RESEARCH
(2021)
Article
Microbiology
Victoria Ballen, Yaiza Gabasa, Carlos Ratia, Melany Sanchez, Sara Soto
Summary: This study characterized 376 extraintestinal pathogenic E. coli strains collected from hospitals in Catalonia, Spain, between 2016 and 2017. The strains showed high resistance to several antibiotics and varied biofilm formation ability. Various virulence and antimicrobial resistance genes were detected, including the colibactin-encoding genes associated with colorectal cancer. The study also investigated the relationship between colibactin and biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Jie Yang, Gang Xiao, Ning Xiao, Zonghan Jiang, Chao Jiang, Yujuan Li, Wenxin Chen, Hongguang Lin, Zhiliang Sun, Jiyun Li
Summary: The plasmid-mediated tigecycline resistance gene tet(X4) was found in Escherichia coli isolates from chicken and pig farms in Hunan province, China. Among the 257 E. coli samples, six tested positive for tet(X4). These tet(X4)-positive strains showed high resistance to multiple drugs, including tigecycline. Whole-genome sequencing and bioinformatics analysis revealed the genomic locations of tet(X4) and identified the plasmid replicon types carrying this gene.
Article
Microbiology
Joao Pires, Laura Huber, Rachel A. Hickman, Simon Dellicour, Kamonwan Lunha, Thongpan Leangapichart, Jatesada Jiwakanon, Ulf Magnusson, Marianne Sunde, Josef D. Jarhult, Thomas P. Van Boeckel
Summary: Thailand is experiencing differences in antimicrobial use and the composition of antimicrobial resistance genes (ARGs) between small and medium farms. Pig isolates from small farms are more associated with genes conferring resistance to colistin and fluoroquinolones, while pig isolates from medium farms are associated with genes conferring resistance to drugs commonly used on medium farms. ARGs conferring resistance to critically important antimicrobials are more frequently co-carried on plasmids from small farms compared to plasmids from medium farms. Actions to curb the spread of these ARGs are urgently needed.
Article
Infectious Diseases
Dennis Carhuaricra, Carla G. Duran Gonzales, Carmen L. Rodriguez Cueva, Yennifer Ignacion Leon, Thalia Silvestre Espejo, Geraldine Marcelo Monge, Raul H. Rosadio Alcantara, Nilton Lincopan, Luis Luna Espinoza, Lenin Maturrano Hernandez
Summary: This study investigated the prevalence of the mcr-1 gene in livestock in Peru and found a high occurrence in pig and chicken farms. The genetic diversity of mcr-1-positive strains calls for a coordinated surveillance approach to effectively detect and limit the spread of colistin-resistant bacteria.
Article
Microbiology
Courtney A. Fancher, Hudson T. Thames, Mary Gates Colvin, Mercedes Smith, Alyssa Easterling, Nikhil Nuthalapati, Li Zhang, Aaron Kiess, Thu T. N. Dinh, Anuraj Theradiyil Sukumaran
Summary: The study investigated the prevalence and virulence of E. coli in antibiotic-free broiler farms, determining that there is a high prevalence of virulent E. coli, especially during the spring season. Antimicrobial resistance was highest for tetracycline, and serogroups O8 and O78 were most frequent in virulent E. coli.
MICROBIOLOGY SPECTRUM
(2021)
Article
Multidisciplinary Sciences
Sarvesh Surve, Dasharath B. Shinde, Ram Kulkarni
Summary: This study performed preliminary phenotypic and genomic characterization of two novel L. plantarum strains isolated from Indian foods and found that the strains exhibited bile and acid tolerance, adherence to intestinal cells, production of exopolysaccharides and folate, and possession of various genetic determinants. Comparative genome analysis showed high variation in cell-adhesion and sugar metabolism genes among 149 L. plantarum strains. This work highlights the unique properties of the studied strains and provides insights into the probiotic and genomic features of L. plantarum strains.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Katharina Meissner, Carola Sauter-Louis, Stefan E. Heiden, Katharina Schaufler, Herbert Tomaso, Franz J. Conraths, Timo Homeier-Bachmann
Summary: Antimicrobial resistance is a growing global problem in the treatment of bacterial infections in animals and humans. This study found that ESBL-positive E. coli, especially of the CTX-M type, were present in both conventional and organic pig farms. The percentage of ESBL-positive pens was higher on conventional farms and varied greatly on organic farms. The farms of origin appeared to have a major influence on the occurrence of ESBL-producing E. coli in the fattening farms.
Article
Virology
Kat R. Smith, Emmanuel W. Bumunang, Jared Schlechte, Matthew Waldner, Hany Anany, Matthew Walker, Kellie MacLean, Kim Stanford, John M. Fairbrother, Trevor W. Alexander, Tim A. Mcallister, Mohamed Faizal Abdul-Careem, Yan D. Niu
Summary: In this study, bacteriophages with the potential to control colibacillosis in chickens were isolated from hen feces and human sewage. These phages showed broad host range, pH tolerance, and no genes associated with virulence or antimicrobial resistance were detected. Comparative genomic analysis and phylogenetic trees revealed the classification and genetic relatedness of the phages. The findings suggest that diverse APEC-infecting phages in the environment could be used for controlling colibacillosis in poultry.
Article
Infectious Diseases
Jonathan Bastard, Nguyen Thi Nhung, Vo Be Hien, Bach Tuan Kiet, Laura Temime, Lulla Opatowski, Juan Carrique-Mas, Marc Choisy
Summary: This study aimed to investigate the within-farm and outside-farm drivers of colistin resistance in small-scale chicken farms in the Mekong delta of Vietnam. The results showed that colistin resistance in chickens was mostly affected by importations of resistance, and to a lesser extent, by recent antimicrobial use.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Agriculture, Dairy & Animal Science
Elise Bernaerdt, Dominiek Maes, Tommy Van Limbergen, Merel Postma, Jeroen Dewulf
Summary: Reduced and responsible antimicrobial use can lower the risk of antimicrobial resistance. This study described the criteria for a Belgian program that raises pigs without antibiotics, and evaluated the possibility of farms achieving and maintaining this status. The study found that there were no significant differences in biosecurity between farms that raised pigs without antibiotics and those that didn't, but biosecurity improved in all farms throughout the study.
Article
Veterinary Sciences
Arife Ezgi Telli, Yusuf Bicer, Nihat Telli, Candan Gungor, Gamze Turkal, Nurhan Ertas Onmaz
Summary: The present study aimed to identify pathogenic Escherichia coli and Salmonella spp. in retail chicken samples and investigate their genetic relation. The results showed a high incidence of E. coli and Salmonella spp. in chicken carcasses, but no virulent strains were detected. The genetic diversity of Salmonella spp. was found to be higher compared to E. coli isolates.
PAKISTAN VETERINARY JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Nelson E. Masota, Knut Ohlsen, Curd Schollmayer, Lorenz Meinel, Ulrike Holzgrabe
Summary: This study isolated and characterized four galloylglucoses with activity against multidrug-resistant Escherichia coli and Klebsiella pneumoniae. These compounds showed potential as antimicrobial agents against Gram-negative bacteria.
Article
Food Science & Technology
Biao Tang, Yan Ma, Xiangxiang He, Qinyi Zhou, Jiang Chang, Mingrong Qian, Xiaodong Xia, Hua Yang
Summary: This study isolated 225 Escherichia coli isolates from chilled chicken samples from markets and chicken fecal samples from farms in Zhejiang Province, China, showing similar antimicrobial resistance patterns, suggesting E. coli strains in markets may originate from farms.
FOODBORNE PATHOGENS AND DISEASE
(2021)
Article
Biology
Jolanta Sarowska, Tomasz Olszak, Agnieszka Jama-Kmiecik, Magdalena Frej-Madrzak, Bozena Futoma-Koloch, Andrzej Gawel, Zuzanna Drulis-Kawa, Irena Choroszy-Krol
Summary: This study analyzed the phylogenetic background, virulence patterns, and drug susceptibility of 132 E. coli isolates and correlated these features with the source of bacterial isolation. The results showed similar virulence patterns between isolates from poultry farms and food, while the isolates from patients with UTI symptoms showed diverse virulence patterns. In terms of drug resistance, the three groups of isolates showed similarity.
Correction
Microbiology
E. Cuevas-Ferrando, P. Guirado, E. Miro, Y. Iglesias-Torrens, F. Navarro, T. S. Alioto, J. Gomez-Garrido, C. Madrid, C. Balsalobre
VETERINARY MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
David Rodriguez-Temporal, Fernando Alcaide, Ivana Marekovic, James Anthony O'Connor, Rebecca Gorton, Jakko van Ingen, An Van den Bossche, Genevieve Hery-Arnaud, Clemence Beauruelle, Dorothea Orth-Hoeller, Juan-Jose Palacios-Gutierrez, Griselda Tudo, German Bou, Pieter-Jan Ceyssens, Montserrat Garrigo, Julia Gonzalez-Martin, Gilbert Greub, Jaroslav Hrabak, Andre Ingebretsen, Maria Concepcion Mediavilla-Gradolph, Marina Oviano, Begona Palop, Arthur B. Pranada, Lidia Quiroga, Maria Jesus Ruiz-Serrano, Belen Rodriguez-Sanchez
Summary: The ability of MALDI-TOF for the identification of nontuberculous mycobacteria (NTM) has been improved recently, thanks to updated databases and optimized protein extraction procedures. This study evaluated the reproducibility of MALDI-TOF in 15 laboratories across 9 European countries for NTM identification. The results showed that MALDI-TOF is a highly reproducible method and suitable for NTM identification implementation.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Eva Gato, Ahalieyah Anantharajah, Manuel J. Arroyo, Maria Jose Artacho, Juan de Dios Caballero, Ana Candela, Katerina Chudejova, Ignacio Pedro Constanso, Cristina Elias, Javier Fernandez, Jesus Jimenez, Pilar Lumbreras, Gema Mendez, Xavier Mulet, Patricia Perez-Palacios, Belen Rodriguez-Sanchez, Rafael Canton, Jaroslav Hrabak, Luis Mancera, Luis Martinez-Martinez, Antonio Oliver, Alvaro Pascual, Alexia Verroken, German Bou, Marina Oviano
Summary: In this study, the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales was evaluated. The method demonstrated high sensitivity and specificity, with an overall agreement rate of 92.5% relative to the reference standard. Results were obtained within 60 minutes and accuracy ranged from 83.3% to 100% among different centers. MALDI-TOF MS proves to be an outstanding tool for rapid detection of carbapenemase activity in clinical microbiology laboratories.
FRONTIERS IN MICROBIOLOGY
(2022)
Review
Infectious Diseases
Maria Nieves Larrosa, Andres Canut-Blasco, Natividad Benito, Rafael Canton, Emilia Cercenado, Fernando Docobo-Perez, Felipe Fernandez-Cuenca, Javier Fernandez-Dominguez, Jesus Guinea, Antonio Lopez-Navas, Miguel Angel Moreno, Ma Isabel Morosini, Ferran Navarro, Luis Martinez-Martinez, Antonio Oliver
Summary: The Spanish Antibiogram Committee presents recommendations on how cumulative antibiogram reports should be prepared in Spanish laboratories of Clinical Microbiology. This article is based on Clinical Microbiology Procedure No. 51 of the Spanish Society of Infectious Diseases and Clinical Microbiology, published in 2014, and includes modifications in clinical interpretive categories recently published by EUCAST in 2019. The objective is to establish a standardized way of preparing these reports for comparing results and conducting local or national surveillance on antimicrobial susceptibility.
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA
(2023)
Article
Infectious Diseases
Pedro Guirado, Elisenda Miro, Yaidelis Iglesias-Torrens, Ferran Navarro, Susana Campoy, Tyler Scott Alioto, Jessica Gomez-Garrido, Cristina Madrid, Carlos Balsalobre
Summary: This study describes the presence of a plasmid and its transferability in a multi-drug resistant strain of Campylobacter jejuni. The plasmid not only carries tetracycline resistance genes, but also a gene cluster that provides resistance to kanamycin and gentamycin. Additionally, the study suggests the possibility of genetic exchange between Campylobacter and distantly related G-positive bacterial genera.
Review
Infectious Diseases
Alba Rivera, Belen Vinado, Natividad Benito, Fernando Docobo-Perez, Felipe Fernandez-Cuenca, Javier Fernandez-Dominguez, Jesus Guinea, Antonio Lopez-Navas, Miguel Angel Moreno, Maria Nieves Larrosa, Antonio Oliver, Ferran Navarro
Summary: Disk diffusion is a standardized method used for guiding antimicrobial therapy. Guidelines from EUCAST, which are widely implemented in Spain, have led to the recommendations by COESANT for antimicrobial selection using the disk diffusion technique.
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA
(2023)
Article
Biotechnology & Applied Microbiology
Pablo Aja-Macaya, Soraya Rumbo-Feal, Margarita Poza, Angelina Canizares, Juan A. A. Vallejo, German Bou
Summary: A new method for extraction and enrichment of genomic MPXV DNA was developed, which significantly improved the sequencing efficiency and trustworthiness of the consensus sequences. Enriched samples contained a higher percentage of MPXV reads compared to non-enriched samples, leading to more accurate and cost-effective diagnosis of contagious diseases.
Article
Infectious Diseases
Juan Carlos Vazquez-Ucha, Isaac Alonso-Garcia, Paula Guijarro-Sanchez, Cristina Lasarte-Monterrubio, Laura Alvarez-Fraga, Arnau Cendon-Esteve, Michelle Outeda, Romina Maceiras, Andrea Pena-Escolano, Marta Martinez-Guitian, Jorge Arca-Suarez, German Bou, Alejandro Beceiro
Summary: Metallo- ,B-lactamase (MBL)-producing Enterobacterales, which are resistant to almost all ,B-lactam antibiotics, pose a significant threat due to their wide dissemination and difficulty in treatment. This study evaluated the activity of aztreonam in combination with novel ,B-lactamase inhibitors (BLIs) against clinical isolates co-producing MBLs and SBLs. The results showed that aztreonam/zidebactam, aztreonam/avibactam, and aztreonam/taniborbactam were the most effective combinations, offering promising therapeutic strategies for difficult-to-treat infections.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Maria Lajarin-Reinares, Eloy Pena-Rodriguez, Mariona Canellas-Santos, Elisabet Rosell-Vives, Pilar Cortes, Montserrat Llagostera Casas, Maria Angels Calvo, Francisco Fernandez-Campos
Summary: Topically applied antimicrobial drugs have several advantages, but the widespread use of antibiotics has led to increasing antimicrobial resistance. Drug repurposing, such as with disulfiram, may provide an attractive alternative for treating multidrug-resistant bacteria in skin infections.
Article
Pharmacology & Pharmacy
Maria Lajarin-Reinares, Iria Naveira-Souto, Mireia Mallandrich, Joaquim Suner-Carbo, Montserrat Llagostera Casas, Maria Angels Calvo, Francisco Fernandez-Campos
Summary: This study aimed to develop and optimize a mucoadhesive drug delivery system for the local administration of disulfiram. The results showed that these gels had antifungal activity against Candida albicans, Candida parapsilosis, and Nakaseomyces glabratus. The physicochemical properties and release profiles of the gels were also characterized and investigated.
Article
Biochemical Research Methods
C. Berengua, E. Miro, C. Gutierrez, M. Sanchez, A. Mulero, P. Ramos, M. del Cuerpo, A. Torrego, I. Garcia-Cadenas, V. Pajares, F. Navarro, R. Martino, N. Rabella
Summary: The purpose of this study was to compare the detection of HCMV in BAL fluid by viral culture and qPCR, and to establish a viral load threshold for identifying cases of pneumonitis. The results showed that 81.25% of the BAL samples had concordant results. Using a cut-off value of 1258 IU/mL of HCMV in BAL, pneumonia can be diagnosed with a sensitivity of 76% and a specificity of 100%.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Immunology
Julia Lopez-Perez, Jennifer Otero, Miquel Sanchez-Osuna, Ivan Erill, Pilar Cortes, Montserrat Llagostera
Summary: This study investigates the emergence of Salmonella strains with reduced susceptibility to a cocktail of three phages in different scenarios. The results show that the mechanisms of resistance vary in different environments, but they do not significantly impair the success of Salmonella biocontrol and oral phage therapy.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Health Care Sciences & Services
Miquel Angel Sastre-Femenia, Almudena Fernandez-Munoz, Maria Antonia Gomis-Font, Biel Taltavull, Carla Lopez-Causape, Jorge Arca-Suarez, Luis Martinez-Martinez, Rafael Canton, Nieves Larrosa, Jesus Oteo-Iglesias, Laura Zamorano, Antonio Oliver
Summary: A high-resolution analysis of resistance patterns, genomic epidemiology, and resistome of Pseudomonas aeruginosa in Spain over a five-year period reveals a general decrease in resistance but an increase in the proportion of XDR strains producing carbapenemases, with the high-risk clone ST235 becoming more prevalent.
LANCET REGIONAL HEALTH-EUROPE
(2023)
Article
Veterinary Sciences
Alice Ferreira, Daniela Silva, Carina Almeida, Maria Elisa Rodrigues, Sonia Silva, Joana Castro, Dalila Mil-Homens, Isidro Garcia-Menino, Azucena Mora, Mariana Henriques, Ana Oliveira
Summary: This study evaluated the efficacy of phage vB_EcoM_FJ1 (FJ1) in reducing the load of pathogenic Escherichia coli (ETEC) and found that encapsulated FJ1 in microparticles significantly reduced the quantity of ETEC. Bacteriophage-insensitive mutants (BIMs) displayed weaker adaptability in the pig model.
VETERINARY RESEARCH
(2023)