Article
Food Science & Technology
Xiaobing Jiang, Siyu Ren, Yimin Geng, Congyi Jiang, Guosheng Liu, Hailei Wang, Tao Yu, Yu Liang
Summary: This study revealed the crucial roles of VirAB and VirS in biofilm formation of L. monocytogenes EGD-e, showing that they are essential for biofilm formation and can function together. While involved in attachment, both VirAB and VirS are not associated with swarming motility. Downregulation of genes flaA, motA, and motB in mutant strains might be the reason for reduced attachment and biofilm formation.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Lili Hu, Qingli Dong, Zhuosi Li, Yue Ma, Muhammad Zohaib Aslam, Yangtai Liu
Summary: This study aimed to determine the effects of different temperature, NaCl concentration, and pH levels on the adhesion and biofilm formation of Listeria monocytogenes, and to build probability models. The results showed that high temperature promoted adhesion and biofilm formation, while excessive NaCl concentration inhibited both processes. The accuracy of the probability models in predicting the adhesion and biofilm formation boundaries of L. monocytogenes ST9 was reasonably high.
Article
Food Science & Technology
Xin Liu, Wenjie Chen, Zhixin Fang, Ying Yu, Jing Bi, Jing Wang, Qingli Dong, Hongzhi Zhang
Summary: This study identified four L. monocytogenes STs in two RTE food plants in Shanghai, China, and found that the biofilm formation ability and resistance to chlorine-containing disinfectants varied among different STs. ST5 and ST121 showed stronger biofilm formation ability and resistance, highlighting the need for stronger surveillance and control measures for these two STs in food processing plants.
Article
Food Science & Technology
Tingting Gu, Apisak Meesrisom, Yaguang Luo, Quynh N. Dinh, Sophia Lin, Manyun Yang, Arnav Sharma, Ruogu Tang, Jinde Zhang, Zhen Jia, Patricia D. Millner, Arne J. Pearlstein, Boce Zhang
Summary: Research showed that different surface topographies and coating compositions significantly impacted the resistance against L. monocytogenes biofilm formation. Coating the native stainless steel surface with Ni-P-PTFE significantly reduced L. monocytogenes contamination. Various coatings on different surfaces were effective in reducing L. monocytogenes contamination, with Dursan showing the best performance on the native surface.
Article
Biotechnology & Applied Microbiology
Christopher T. Gemmell, Valeria R. Parreira, Jeffrey M. Farber
Summary: The aim of this study was to investigate the ability of natural plant-derived products (flavonoid compounds) to inhibit the growth and biofilm-forming ability of Listeria monocytogenes. Among 500 flavonoid compounds, five were identified as promising antibiofilm and antimicrobial agents against Listeria monocytogenes.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Food Science & Technology
Zhen Wang, Yue Ma, Zhuosi Li, Yuan Wang, Yangtai Liu, Qingli Dong
Summary: This study investigated the spatial and temporal differences during the formation of L. monocytogenes biofilms, as well as the transfer and residual of biofilm cells at different stages. The results showed that the transfer rates of biofilm cells had no significant difference between single and ten successive contacts at specific stages, and the transfer rates were correlated with the hydrophobicity of biofilm cells.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Biotechnology & Applied Microbiology
Saili Chalke, Sinisa Vidovic, Graham C. Fletcher, Jon Palmer, Steve Flint
Summary: In this study, the influence of magnesium, calcium, and sodium on biofilm formation in four isolates of L. monocytogenes from seafood processing environments was investigated. The study showed that magnesium and calcium increased biofilm formation, while sodium had a lesser effect. These cations appeared to have a greater influence on the later stages of biofilm development.
Article
Microbiology
Lixia Wang, Chunhui Ji, Xianzhu Xia, Xuepeng Cai, Qingling Meng, Jun Qiao
Summary: This study investigated the regulatory roles and mechanisms of sRNA Rli43 in Listeria monocytogenes. The results showed that Rli43 plays important roles in environmental adaptation, biofilm formation, and virulence modulation in L. monocytogenes.
Article
Biotechnology & Applied Microbiology
Mohit Bansal, Nitin Dhowlaghar, Ramakrishna Nannapaneni, Divya Kode, Sam Chang, Chander S. Sharma, Christopher McDaniel, Aaron Kiess
Summary: The study demonstrates that subinhibitory concentrations of NaOCl can reduce the ability of L. monocytogenes planktonic cells to form biofilms on polystyrene surfaces and induce morphological changes in the biofilm structure and attachment capabilities. Additionally, subinhibitory NaOCl also downregulates the expression of biofilm formation-related genes in L. monocytogenes.
Article
Food Science & Technology
Qingli Dong, Linjun Sun, Taisong Fang, Yuan Wang, Zhuosi Li, Xiang Wang, Mengjie Wu, Hongzhi Zhang
Summary: This study investigated the mono- and dual-species biofilm formation of L. monocytogenes and P. aeruginosa under different culture conditions. Results showed the inhibitory effect of chicken juice on L. monocytogenes biofilm formation, as well as the dominance of P. aeruginosa in dual-species biofilms in certain culture mediums. The correlation between motility and biofilm formation was found to be insignificant.
Article
Food Science & Technology
Qian Guo, Yu Zhang, Xiaowei Fang, Yuying Yang, Xiongyan Liang, Jing Liu, Chun Fang
Summary: Listeria monocytogenes is a ubiquitous foodborne pathogen that forms persistent biofilms in food processing environments, posing a significant threat to human health and food safety. The two-component system virS/virR positively regulates motility and biofilm formation in L. monocytogenes, and its deletion reduces both traits. This study provides valuable insights for controlling biofilm risks of L. monocytogenes in food safety.
Article
Food Science & Technology
Yuan Wang, Linjun Sun, Lili Hu, Zhen Wang, Xiang Wang, Qingli Dong
Summary: According to the study, the nutrition status affects the adhesion ability and biofilm formation of Listeria monocytogenes. Nutrient deficiency can enhance the adhesion and biofilm formation of the bacteria, and also lead to changes in the expression of critical biofilm-associated genes. These findings are important for understanding the spread and control of Listeria in food processing.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Microbiology
Pan Mao, Yan Wang, Lin Gan, Lingyun Liu, Jinni Chen, Lingling Li, Hui Sun, Xia Luo, Changyun Ye
Summary: In this study, a comprehensive genetic analysis of sigB in a dataset of 46,921 L. monocytogenes genomes was conducted. Two predominant SigB factors (SigB(T1) and SigB(T2)) were identified, with a strong correlation between these factors and different lineages of L. monocytogenes. SigB(T1) showed superior performance in promoting cellular invasion, cytotoxicity, biofilm formation, and cold tolerance compared to SigB(T2). These findings highlight the important role of distinct SigB factors in influencing the biological traits of L. monocytogenes of different lineages, emphasizing their unique pathogenic and adaptive attributes.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
J. Nowak, S. B. Visnovsky, C. D. Cruz, G. C. Fletcher, A. H. M. van Vliet, D. Hedderley, R. Butler, S. Flint, J. Palmer, A. R. Pitman
Summary: The study revealed that biofilm formation and gallidermin resistance of L. monocytogenes are influenced by the mprF gene, with its inactivation leading to enhanced biofilm formation and abiotic surface attachment but compromising invasiveness.
JOURNAL OF APPLIED MICROBIOLOGY
(2021)
Article
Microbiology
Xiaobing Jiang, Congyi Jiang, Tao Yu, Xiaojie Jiang, Siyu Ren, Rui Kang, Shuxing Qiu
Summary: BC adaptation enhances biofilm formation but reduces swarming motility in L. monocytogenes. The expression of flagella-mediated motility-related genes is downregulated in BC adapted strains. BC adaptation increases the ability of L. monocytogenes to adhere to and invade Caco-2 cells, without affecting hemolytic activity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Ping Lu, Shuyi Wang, Yan Lu, Dante Neculai, Qiming Sun, Stijn van der Veen
JOURNAL OF INFECTIOUS DISEASES
(2019)
Article
Infectious Diseases
Jing Yan, Juan Xue, Yan Chen, Shi Chen, Qiang Wang, Chuanling Zhang, Shenghai Wu, Huoyang Lv, Yunsong Yu, Stijn van der Veen
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Letter
Infectious Diseases
Fan Yang, Haifang Zhang, Yan Chen, Yunxia Zhai, Feng Zhao, Yunsong Yu, Stijn van der Veen
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Infectious Diseases
Fan Yang, Jing Yan, Jianglin Zhang, Stijn van der Veen
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2020)
Article
Infectious Diseases
Shao-Chun Chen, Liu-Feng Yuan, Xiao-Yu Zhu, Stijn van der Veen, Yue-Ping Yin
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2020)
Article
Immunology
Ke Zhou, Shao-Chun Chen, Fan Yang, Stijn Van der Veen, Yue-Ping Yin
EMERGING MICROBES & INFECTIONS
(2020)
Article
Immunology
Juan Zhang, Shuyi Wang, Tjakko Abee, Stijn van der Veen
Summary: This study highlights the importance of base excision repair (BER) for Listeria monocytogenes virulence and survival during host colonization. By deleting specific DNA glycosylases and apurinic/apyrimidinic endonucleases, researchers found that certain mutants were attenuated in virulence in a mouse model and showed reduced colonization in the liver and spleen.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Genetics & Heredity
Chunting Hua, Jiang Zhu, Boya Zhang, Siyuan Sun, Yinjing Song, Stijn van der Veen, Hao Cheng
FRONTIERS IN GENETICS
(2020)
Article
Multidisciplinary Sciences
Wen-Ge Li, Chang-Hui Cai, Xin-Chao Cai, Shao-Chun Chen, Stijn van der Veen, Yue-Ping Yin
Article
Infectious Diseases
Jing Yan, Yan Chen, Fan Yang, Xia Ling, Shengnan Jiang, Feng Zhao, Yunsong Yu, Stijn van der Veen
Summary: This study found a high percentage of ceftriaxone-resistant FC428 clone among clinical isolates from a single hospital in Hangzhou, China, with seven isolates displaying high-level ceftriaxone resistance and treatment failure potentially linked to initial prescription of oral cephalosporins. Retreatment with a higher dose of IV ceftriaxone appeared successful in these cases.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Qiang Wang, Xiaofang Tang, Stijn van der Veen
Summary: This case report describes a surgical patient with Mycoplasma hominis bloodstream infection and pneumonia. After unsuccessful treatment with standard antimicrobial therapy, the pathogen was identified through culturing and sequencing, and combination therapy based on antimicrobial susceptibility testing was administered. Importantly, a prostate abscess was identified as a possible primary source of infection.
BMC INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Rajesh Varkhedkar, Fan Yang, Rakesh Dontha, Jianglin Zhang, Jiyong Liu, Bernhard Spingler, Stijn van der Veen, Simon Duttwyler
Summary: The identification of an alternative chemical space is crucial for discovering novel antimicrobial lead compounds to address the global challenge of antimicrobial resistance. Boron clusters are being explored due to their unique properties, but their synthesis and derivatization techniques have limitations. A new method was developed to rapidly construct a library of molecules and evaluate their antibacterial activity.
ACS CENTRAL SCIENCE
(2022)
Article
Cell Biology
Chunting Hua, Qiaoli Zheng, Jiang Zhu, Siji Chen, Yinjing Song, Stijn van der Veen, Hao Cheng
Summary: This study found that high-risk human papillomavirus 16 infection is associated with activation of the mitogen activated protein kinase (MAPK)/ERK signaling pathway and induction of canonical autophagy. Additionally, the expression of dual-specificity phosphates 5 (DUSP5) is repressed by HPV16 E7. These findings suggest that DUSP5 may serve as a potential therapeutic target for cervical cancer.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2022)
Article
Microbiology
Shuai Gao, Abidullah Khan, Xuhong Chen, Guohui Xiao, Stijn van der Veen, Yin Chen, Xu'ai Lin
Summary: This study demonstrates that c-di-GMP can stimulate immune responses and reduce MRSA infections by enhancing the secretion of antimicrobial peptides and chemokines. In a mouse skin wound infection model, pre-treatment with c-di-GMP significantly reduced bacterial colonization and promoted inflammatory cell infiltration, making it more difficult for MRSA to establish an infection.
Article
Immunology
Lingyu Gao, Zhemin Wang, Stijn van der Veen
Summary: The study elucidated the mechanisms of Neisseria gonorrhoeae resistance to palmitic acid. A stable palmitic acid-resistant strain obtained through serial passage outcompeted its parent strain in a murine infection model, with resistance-related SNPs and determinants enhancing gene expression of the FarAB efflux pump and enzyme activity of the FadD enzyme.
JOURNAL OF INFECTIOUS DISEASES
(2021)
