Article
Multidisciplinary Sciences
Patricia Lucas-Elio, Luisa Raquel Molina-Quintero, Hengyi Xu, Antonio Sanchez-Amat
Summary: This study reveals that Marinomonas mediterranea MMB-1 possesses two CRISPR-Cas systems that are controlled by the histidine kinase PpoS and a response regulator PpoR, with similarities to global regulators BarA/UvrY. RNA-Seq analyses show the involvement of these genetic elements in a regulatory cascade, and mutants in PpoS and PpoR display increased sensitivity to phages, indicating the important role of regulation by these proteins in phage defense.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Neda Baseri, Shahin Najar-Peerayeh, Bita Bakhshi
Summary: The WalK-H364R mutation in VISA strain destabilized the protein and decreased interactions with other molecules, potentially contributing to VISA development. Downregulation of related genes was observed, supporting the impact of this mutation. This study highlights the importance of molecular modelling in uncovering the molecular mechanisms of VISA development.
Article
Immunology
Won-Sik Yeo, Sophie Dyzenhaus, Victor J. Torres, Shaun R. Brinsmade, Taeok Bae
Summary: The regulation of membrane protein activity is dependent on the composition of phospholipid membranes. Cardiolipin, found in bacterial and mitochondrial membranes, plays a crucial role in stabilizing membrane proteins and maintaining their function. In Staphylococcus aureus, cardiolipin is critical for sustaining the activity of the SaeRS two-component system and other sensor kinases during infection.
INFECTION AND IMMUNITY
(2023)
Article
Agronomy
Helber Enrique Balaguera-Lopez, Mauricio Espinal-Ruiz, Jeimmy Marcela Rodriguez-Nieto, Anibal Herrera-Arevalo, Lorenzo Zacarias
Summary: The study found that 1-MCP acted as an inhibitor of ethylene biosynthesis and activity in cape gooseberry fruits, affecting volatile compound emission, color index, and firmness loss. It acted as a competitive inhibitor of ACC oxidase and a noncompetitive inhibitor of HK, with a higher coupling free energy compared to their substrates.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Mehul V. Makwana, Mike P. Williamson, Richard F. W. Jackson, Richmond Muimo
Summary: There is growing evidence that phosphohistidines play a significant role in regulating cellular activity, particularly in cancer-related signaling pathways. A new protocol for protein extraction and P-31 NMR spectroscopic analysis was used to measure phosphohistidine concentrations in human bronchial epithelial cells. The results show that phosphohistidine is about one third as abundant as phosphoserine and phosphothreonine combined, making it approximately 15 times more abundant than phosphotyrosine. These findings strengthen the case for a functional role of phosphohistidine in eukaryotic cells.
Article
Biochemistry & Molecular Biology
So Yeon Cho, Sung-il Yoon
Summary: Vibrio parahaemolyticus VbrK(SD) detected beta-lactam antibiotics or underwent S-nitrosylation in response to host nitrites. The distal end of VbrK(SD) plays a key role in initiating the VbrK-VbrR TCS pathway activation and is involved in the nitrosylation-mediated regulation of the structural dynamics of VbrK. The structural features of VbrK(SD) that are indispensable for biological functions may be conserved in other VbrK orthologs.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Biotechnology & Applied Microbiology
Rodrigo Cruz-Bautista, Beatriz Ruiz-Villafan, Alba Romero-Rodriguez, Romina Rodriguez-Sanoja, Sergio Sanchez
Summary: Despite advances in understanding the regulatory networks for secondary metabolite production, the participation of two-component systems (TCS) in this process requires better characterization. Defining the stimulus that triggers TCS activation is still a challenge due to the transmembrane nature of sensor kinases and the high GC content in streptomycetes. Obtaining sufficient sensor histidine kinase concentrations could facilitate the identification of ligand-protein interactions and the determination of phosphorylation mechanisms and tridimensional structure.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Muhammad Junaid, Yu Inaba, Ana Otero, Iwane Suzuki
Summary: Histidine kinases are sensory proteins commonly used in bacteria to detect environmental changes, while transcriptional regulation in cyanobacteria is important for production of useful compounds without altering growth profiles. A chimeric sensor, VanN_SphS, was developed to achieve reversible transcriptional regulation in cyanobacteria, showing potential for controlled production of useful compounds.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Microbiology
Lingyuan Kong, Mingyang Su, Jiayan Sang, Shanshan Huang, Min Wang, Yongfei Cai, Mingquan Xie, Jun Wu, Shida Wang, Simon J. Foster, Jiaqin Zhang, Aidong Han
Summary: In Streptococcus mutans, the histidine kinase Walk's long C-terminal tail is crucial for its interaction with the response regulator WaIR, and plays a key role in signal transduction, transcriptional regulation, and biofilm formation.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Tatsuro Yoneyama, Abdelsamed Elshamy, Junpei Yamada, Walaa A. El-Kashak, Yusuke Kasai, Hiroshi Imagawa, Sayaka Ban, Masaaki Noji, Akemi Umeyama
Summary: Cordyceps spp. is a widely recognized healthy food with traditional uses and bio-functionalities. Chemical characterization of an entomopathogenic fungus extract led to the identification of two new metabolites and four known metabolites. One of the metabolites exhibited antimicrobial activity and significant binding affinities to specific proteins.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Review
Immunology
Biaoyou Liao, Xingchen Ye, Xi Chen, Yujie Zhou, Lei Cheng, Xuedong Zhou, Biao Ren
Summary: The two-component system of Candida albicans plays crucial roles in regulating various physiological processes and could serve as a potential target for new antifungal agents.
Article
Chemistry, Analytical
Huachao Che, Nengneng Li, Yulun Nie, Xike Tian, Yong Li, Liqiang Lu, Yongqiang Wang
Summary: A convenient method for simultaneous detection of As(III/V), Cr(III/VI), and Fe(II/III) has been developed using a sensor array. The array showed low detection limit and could achieve simultaneous qualitative and quantitative results for composite samples of multiple metal ions.
Article
Chemistry, Multidisciplinary
Agnidipta Ghosh, Courtney N. Niland, Sean M. Cahill, Nishant M. Karadkhelkar, Vern L. Schramm
Summary: Human methionine adenosyltransferase MAT2A plays a crucial role in providing S-adenosyl-L-methionine (AdoMet) for methyl-transfer reactions, influencing expression patterns in development and cancer. Studies describe the mechanism of AdoMet and triphosphate formation, as well as the hydrolysis process of triphosphate to pyrophosphate and phosphate by MAT2A. Crystallographic analysis reveals the catalytic site geometry of the transition-state reconstruction, predicting a loose transition state with characteristics of symmetric nucleophilic displacement.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Multidisciplinary Sciences
Renu A. Kowluru, Rakesh Radhakrishnan, Ghulam Mohammad
Summary: This study investigates the role of histone methylation (H3K9me3) at the Rac1 promoter in dynamic DNA methylation-transcriptional activation. The results show that H3K9me3 at the Rac1 promoter assists in active DNA methylation-hydroxymethylation, activating Rac1 transcription, which may prevent the development of diabetic retinopathy.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Andrei Shelud'ko, Irina Volokhina, Dmitry Mokeev, Elizaveta Telesheva, Stella Yevstigneeva, Andrei Burov, Anna Tugarova, Alexander Shirokov, Gennady Burigin, Larisa Matora, Lilia Petrova
Summary: This study investigated the two-component signal transduction system (TCS) in A. baldaniorum bacteria, which regulates their response to mechanical stress and osmotic/water stress. The mutant showed changes in bacterial morphology, motility, and secretion apparatus. Under osmotic stress, the mutant lost its collective motility.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Esha Sehanobish, Jonatan C. Campillo-Brocal, Heather R. Williamson, Antonio Sanchez-Amat, Victor L. Davidson
Article
Biochemical Research Methods
Fabio Tonin, Roberta Melis, Arno Cordes, Antonio Sanchez-Amat, Loredano Pollegioni, Elena Rosini
Article
Biochemical Research Methods
Fabio Tonin, Elena Rosini, Luciano Piubelli, Antonio Sanchez-Amat, Loredano Pollegioni
PROTEIN EXPRESSION AND PURIFICATION
(2016)
Article
Biology
Sukrit Silas, Patricia Lucas-Elio, Simon A. Jackson, Alejandra Aroca-Crevillen, Loren L. Hansen, Peter C. Fineran, Andrew Z. Fire, Antonio Sanchez-Amat
Article
Biochemistry & Molecular Biology
Andres Andreo-Vidal, Kyle J. Mamounis, Esha Sehanobish, Dante Avalos, Jonatan Cristian Campillo-Brocal, Antonio Sanchez-Amat, Erik T. Yukl, Victor L. Davidson
Article
Chemistry, Medicinal
Andres Andreo-Vidal, Antonio Sanchez-Amat, Jonatan C. Campillo-Brocal
Article
Biochemistry & Molecular Biology
Georg Mohr, Sukrit Silas, Jennifer L. Stamos, Kira S. Makarova, Laura M. Markham, Jun Yao, Patricia Lucas-Elio, Antonio Sanchez-Amat, Andrew Z. Fire, Eugene Koonin, Alan M. Lambowitz
Article
Biochemistry & Molecular Biology
Kyle J. Mamounis, Zhongxin Ma, Antonio Sanchez-Amat, Victor L. Davidson
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2019)
Article
Multidisciplinary Sciences
Patricia Lucas-Elio, Luisa Raquel Molina-Quintero, Hengyi Xu, Antonio Sanchez-Amat
Summary: This study reveals that Marinomonas mediterranea MMB-1 possesses two CRISPR-Cas systems that are controlled by the histidine kinase PpoS and a response regulator PpoR, with similarities to global regulators BarA/UvrY. RNA-Seq analyses show the involvement of these genetic elements in a regulatory cascade, and mutants in PpoS and PpoR display increased sensitivity to phages, indicating the important role of regulation by these proteins in phage defense.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Kyle J. Mamounis, Maria Luiza Caldas Nogueira, Daniela Priscila Marchi Salvador, Andres Andreo-Vidal, Antonio Sanchez-Amat, Victor L. Davidson
Summary: The Pseudoalteromonas luteoviolacea strain CPMOR-1 expresses a flavin adenine dinucleotide (FAD)-dependent L-amino acid oxidase (LAAO) with broad substrate specificity. This study investigated the reactivity of the enzyme towards proteinogenic amino acids and found that its activity towards different amino acids is influenced by their binding affinity and relative positioning in the active site. Comparison of the enzyme's structural model with other FAD-dependent LAAOs revealed that specific residues in the active site play a role in determining the enzyme's substrate specificity.
Article
Biology
Sukrit Silas, Nimit Jain, Michael Stadler, Becky Xu Hua Fu, Antonio Sanchez-Amat, Andrew Z. Fire, Joshua Arribere
Article
Biochemistry & Molecular Biology
Heather R. Williamson, Esha Sehanobish, Alan M. Shiller, Antonio Sanchez-Amat, Victor L. Davidson