Article
Microbiology
Manal Mohammed, Beata Orzechowska
Summary: The study aimed to characterize phage resistance mechanisms and genomic differences in S. Typhimurium DT104 and DT104b that may explain divergent phage reaction patterns, but found no unique genetic determinants. Experimental assessment of phage-specific receptors on the bacterial cell surface and analysis of bacterial transcriptome using RNA sequencing were suggested to further understand bacterial susceptibility to phages. The use of Anderson phage typing scheme of Salmonella Typhimurium can help improve the understanding of host-phage interactions and lead to the development of phage-based technologies for infection control.
Article
Food Science & Technology
Rui Dong, Xiaojie Qin, Shoukui He, Xiujuan Zhou, Yan Cui, Chunlei Shi, Yiping He, Xianming Shi
Summary: The study revealed that DsrA plays a crucial role in promoting oxidative stress resistance in S. Typhimurium by regulating the expression of antioxidant enzymes and maintaining intracellular redox balance.
Article
Food Science & Technology
Zhongyue Ren, Lingling Peng, Shufang Chen, Yi Pu, Huihui Lv, Hua Wei, Cuixiang Wan
Summary: Salmonella Typhimurium is commonly found in food and can cause infection in the gastrointestinal tract. This study investigated the effect of a high-fat diet (HFD) on S. Typhimurium infection and the potential protective effects of Lactiplantibacillus plantarum 1201 intervention. The results showed that HFD exacerbated the infection, while intervention with L. plantarum 1201 effectively reduced colonization and inflammation. These findings suggest that L. plantarum 1201 may be a potential preventive strategy against S. Typhimurium infection.
Article
Microbiology
Yu Han, Yu Li, Zhiwei Zeng, Wenjing Li, Saixiang Feng, Weisheng Cao
Summary: This study explores the resistance mechanism and activity of the antimicrobial peptide MccY against Salmonella strains. The deletion of Ton system genes resulted in resistance to MccY and mutants showed increased susceptibility to MccY under a low-iron condition. MccY may decrease the virulence of S. Typhimurium by altering multiple physiological properties.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Analytical
Han Du, Xu Zhang, Mingru Yao, Qingli Yang, Wei Wu
Summary: This study successfully constructed aptamer-guided luminous microspheres using carbon dots fluorescence, which can efficiently recognize and detect Salmonella enterica serovar Typhimurium. The method shows high sensitivity and has broad application prospects.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Immunology
Fabio Fiorino, Elena Pettini, Oliver Koeberling, Annalisa Ciabattini, Gianni Pozzi, Laura B. Martin, Donata Medaglini
Summary: STm vaccine based on GMMA technology can induce long-lasting anti-bacterial immunity in mice and have a preventive effect on iNTS disease.
Article
Microbiology
Jingyan Shu, Hongtao Liu, Yang Liu, Xindi Chen, Yu Yu, Qianghua Lv, Jianfeng Wang, Xuming Deng, Zhimin Guo, Jiazhang Qiu
Summary: Tannic acid (TA) has been shown to improve survival rates and alleviate cecal pathological lesions in S. Typhimurium-infected mice, while inhibiting bacterial invasion of HeLa cells. TA may exert its anti-infection effects by controlling the expression of key genes in the T3SS.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Stephanie Paulini, Florian D. Fabiani, Anna S. Weiss, Ana Laura Moldoveanu, Sophie Helaine, Baerbel Stecher, Kirsten Jung
Summary: Pyruvate, the simplest of the alpha-keto acids, plays a crucial role in the metabolic pathways of bacteria and eukaryotes. This study identified two specific transporters, BtsT and CstA, in Salmonella enterica serovar Typhimurium, which are important for pyruvate uptake and have significant effects on bacterial survival, infection, and persistence.
Article
Nutrition & Dietetics
Jin Zhou, Tingyang Wang, Lele Fan, Hongde Xiao, Hui Ji, Naiji Zhou, Zutao Zhou, Huazhen Liu, Muhammad Akhtar, Yuncai Xiao, Deshi Shi
Summary: This study observed that the administration of Enterococcus faecium HDRsEf1 (HDRsEf1) improved the development of the immune system by increasing the spleen index and the area of spleen white pulp. The proportion of T helper (Th) 1 cells and the production of IFN-γ and IL-12 were significantly increased in mice treated with HDRsEf1, both in vivo and in splenocytes. HDRsEf1 also enhanced resilience against systemic infection and stimulated the expression of genes for TNF alpha and iNOS, indicating the activation of macrophages for antibacterial defenses.
Article
Microbiology
Jiwon Baek, Hyunjin Yoon
Summary: Cyclic di-GMP is a signaling molecule that controls the transition between motile and nonmotile lifestyles in bacteria. It regulates biofilm formation and dispersal, and it can alter carbon metabolic pathways in Salmonella enterica serovar Typhimurium.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biotechnology & Applied Microbiology
Yucen Xie, Nitin Nitin, Linda J. Harris
Summary: Factors influencing bacterial transfer between yellow onions and polyurethane or stainless steel surfaces under dry conditions were investigated. The study found that transfer rates were significantly dependent on bacterial species, inoculation levels, inoculum carrier, and transfer direction. The results showed that Rifampin-resistant Enterococcus faecium had higher transfer rates than Salmonella, and transfer from onion to polyurethane was higher than from polyurethane to onion.
Article
Microbiology
R. Kavi Bharathi, C. S. Srinandan, N. Sai Subramanian
Summary: This study investigates the cooperative ability and matrix sharing between specialist and generalist strains during biofilm formation. The results show that the matrix aids in survival during antibiotic, chlorine, and predatory stress, and possible sharing of the matrix occurs in co-culture.
Article
Microbiology
Seul Kim, Eunsuk Kim, Hyunjin Yoon
Summary: Salmonella alters cellular processes to improve intracellular fitness during host infection by rewiring cellular transcriptional regulation through alternative sigma factors such as rpoS. Deprivation of outer membrane protein YcfR in Salmonella Typhimurium leads to decreased intracellular survival and downregulation of SPI-2 genes, which is abolished in the absence of rpoS. RpoS-associated stress responses in Salmonella due to impaired envelope integrity may reciprocally downregulate the expression of SPI-2 genes to reduce its virulence.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Yujing Shao, Wenrui Zhen, Fangshen Guo, Zeqiong Hu, Kaichen Zhang, Linhua Kong, Yuming Guo, Zhong Wang
Summary: The study showed that supplementation of E. faecium NCIBM 11181 could alleviate gut injury induced by S. Typhimurium infection, and may serve as an effective non-antibiotic feed additive for improving gut health and controlling Salmonella infection in broiler chickens.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Petra Geiser, Maria Letizia Di Martino, Pilar Samperio Ventayol, Jens Eriksson, Eduardo Sima, Anas Kh. Al-Saffar, David Ahl, Mia Phillipson, Dominic-Luc Webb, Magnus Sundbom, Per M. Hellstrom, Mikael E. Sellin
Summary: This study reveals the complete infection cycle of Salmonella enterica serovar Typhimurium within human and mouse enteroids, and demonstrates how bacterial invasion of the epithelium strongly fuels expansion in the luminal compartment through a mechanism involving the death and expulsion of bacterium-infected epithelial cells.
Article
Psychology, Developmental
Ulrike Ravens-Sieberer, Anne Kaman, Michael Erhart, Christiane Otto, Janine Devine, Constanze Loeffler, Klaus Hurrelmann, Monika Bullinger, Claus Barkmann, Nico A. Siegel, Anja M. Simon, Lothar H. Wieler, Robert Schlack, Heike Hoelling
Summary: The German COPSY study is a longitudinal study that examines the mental health impact of the COVID-19 pandemic on children and adolescents. The study found that during the pandemic, the health-related quality of life (HRQoL) of children and adolescents decreased, and there was an increase in emotional problems, peer-related mental health problems, anxiety, depressive symptoms, and psychosomatic complaints. Socially disadvantaged children and children of mentally burdened parents were more at risk of impaired mental health, while female gender and older age were associated with fewer mental health problems. A positive family climate and social support supported the mental health of children and adolescents during the pandemic.
EUROPEAN CHILD & ADOLESCENT PSYCHIATRY
(2023)
Article
Public, Environmental & Occupational Health
Andre Hajek, Freia De Bock, Christina Merkel, Sarah Eitze, Cornelia Betsch, Michael Bosnjak, Lothar H. Wieler, Hans-Helmut Koenig
Summary: This study aimed to shed light on attitudes towards influenza vaccination in Germany in 2021/2022 based on the COSMO survey. The results showed that about one-third of respondents and healthcare workers plan to get a flu shot this year. Among the at-risk group of people aged 60 and older, more than half plan to get vaccinated. Correlates, such as gender, were identified, with women having a lower likelihood of planning to get a flu shot. The study concludes that physicians should inform women, especially during the pandemic, about the advantages of influenza vaccination and communicate the proven protective effect convincingly.
Article
Microbiology
Sebastian Braetz, Peter Schwerk, Arthur Thompson, Karsten Tedin, Marcus Fulde
Summary: The efficacy of killing by bactericidal antibiotics is largely influenced by ATP levels. This study found that an ATP synthase-deficient strain of Salmonella enterica serovar Typhimurium was more sensitive to the fluoroquinolone antibiotic and showed increased survival or no significant difference when challenged with other antibiotics. The increased cell killing and reduced persistence after fluoroquinolone challenge were a result of metabolic compensation through central carbon metabolism reactions and increased NAD(P)H levels.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Gastroenterology & Hepatology
Fereshteh Ghazisaeedi, Jochen Meens, Bianca Hansche, Sven Maurischat, Peter Schwerk, Ralph Goethe, Lothar H. Wieler, Marcus Fulde, Karsten Tedin
Summary: The probiotic bacterial strain Enterococcus faecium SF68 has been found to alleviate intestinal inflammation and modulate the immune response. The inhibitory effect on host cell signaling pathways is likely due to the presence of arginine deiminase, which is responsible for the anti-inflammatory effects observed in clinical trials.
Article
Multidisciplinary Sciences
Hannelore Neuhauser, Angelika Schaffrath Rosario, Hans Butschalowsky, Sebastian Haller, Jens Hoebel, Janine Michel, Andreas Nitsche, Christina Poethko-Mueller, Franziska Pruetz, Martin Schlaud, Hans W. Steinhauer, Hendrik Wilking, Lothar H. Wieler, Lars Schaade, Stefan Liebig, Antje Gosswald, Markus M. Grabka, Sabine Zinn, Thomas Ziese
Summary: This study from Germany provides data on pre-vaccine SARS-CoV-2 seroprevalence and testing, highlighting socioeconomic disparities and low detection rates.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Sumeet K. Tiwari, Boas C. L. van der Putten, Thilo M. Fuchs, Trung N. Vinh, Martin Bootsma, Rik Oldenkamp, Roberto La Ragione, Sebastien Matamoros, Ngo T. Hoa, Christian Berens, Joy Leng, Julio Alvarez, Marta Ferrandis-Vila, Jenny M. Ritchie, Angelika Fruth, Stefan Schwarz, Lucas Dominguez, Maria Ugarte-Ruiz, Astrid Bethe, Charlotte Huber, Vanessa Johanns, Ivonne Stamm, Lothar H. Wieler, Christa Ewers, Amanda Fivian-Hughes, Herbert Schmidt, Christian Menge, Torsten Semmler, Constance Schultsz
Summary: This study analyzes the genetic determinants of host specificity in E. coli and identifies specific genes associated with different hosts. The nan-9 gene cluster shows a strong association with the human host, while previously known sialic acid regulon genes show no significant association with any host.
Article
Public, Environmental & Occupational Health
Simone Scheithauer, Alexander Dilthey, Anna Bludau, Sandra Ciesek, Victor Corman, Tjibbe Donker, Tim Eckmanns, Richard Egelkamp, Hajo Grundmann, Georg Haecker, Martin Kaase, Berit Lange, Alexander Mellmann, Martin Mielke, Mathias Pletz, Bernd Salzberger, Andrea Thuermer, Andreas Widmer, Lothar H. Wieler, Thorsten Wolff, Soeren Gatermann, Torsten Semmler
Summary: The deficit of genomic pathogen surveillance infrastructure in Germany has been revealed during the SARS-CoV-2 pandemic. Establishing an efficient and adaptable genomic pathogen surveillance network is urgently needed to prepare for future pandemics. This paper proposes measures based on global and country-specific best practices to achieve integrated genomic pathogen surveillance, which includes linking epidemiological data with pathogen genomic data, sharing and coordinating resources, making surveillance data available to decision-makers, public health service, and the scientific community, and engaging all stakeholders.
BUNDESGESUNDHEITSBLATT-GESUNDHEITSFORSCHUNG-GESUNDHEITSSCHUTZ
(2023)
Article
Public, Environmental & Occupational Health
Christopher Irrgang, Tim Eckmanns, Max von Kleist, Esther-Maria Antao, Katharina Ladewig, Lothar Wieler, Nils Koerber
Summary: Societal health is facing new challenges due to climate change, demographic ageing, and globalization. The One Health approach aims to understand health holistically by linking human, animal, and environmental sectors. Artificial intelligence techniques offer opportunities for cross-sectoral assessment of health threats. This article discusses the applications and challenges of AI techniques in the context of One Health, using antimicrobial resistance as an example.
BUNDESGESUNDHEITSBLATT-GESUNDHEITSFORSCHUNG-GESUNDHEITSSCHUTZ
(2023)
Article
Infectious Diseases
Bernd Salzberger, Alexander Mellmann, Anna Bludau, Sandra Ciesek, Victor Corman, Alexander Dilthey, Tjibbe Donker, Tim Eckmanns, Richard Egelkamp, Soeren G. Gatermann, Hajo Grundmann, Georg Haecker, Martin Kaase, Berit Lange, Martin Mielke, Mathias W. Pletz, Torsten Semmler, Andrea Thuermer, Lothar H. Wieler, Thorsten Wolff, Andreas F. Widmer, Simone Scheithauer
Summary: The COVID-19 pandemic has highlighted the importance of effective infection surveillance and infrastructure. Germany's genomic pathogen sequencing infrastructure was inadequate due to a lack of equipment, resources, data management, and coordination. The authors propose the establishment of comprehensive genomic pathogen surveillance in Germany, including sequencing, data collection, data linkage, and target pathogens, to better address future epidemics and pandemics and learn from the lessons of the COVID-19 pandemic.
Article
Infectious Diseases
Mareike Saathoff, Karsten Tedin, Stefan Graetz, Peter Schwerk, Maria Kunert, Roderich D. Suessmuth, Marcus Fulde
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Public, Environmental & Occupational Health
Carmen Koschollek, Katja Kajikhina, Charbel El Bcheraoui, Lothar H. Wieler, Niels Michalski, Claudia Hovener
Summary: The study aimed to investigate the risk of SARS-CoV-2 infection among migrants and ethnic minorities in Germany, and discuss potential explanations for these associations. The results showed that the risk of SARS-CoV-2 infection is determined by socio-economic factors rather than migrant status.
Article
Microbiology
Sebastian Braetz, Peter Schwerk, Nara Figueroa-Bossi, Karsten Tedin, Marcus Fulde
Summary: Our study demonstrates that prophages significantly reduce persister cell formation in lysogenic cells exposed to DNA-gyrase-targeting drugs, and also impact the initial drug susceptibility.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Ruth Offergeld, Karina Preussel, Thomas Zeiler, Konstanze Aurich, Barbara I. Baumann-Baretti, Sandra Ciesek, Victor M. Corman, Viktoria Dienst, Christian Drosten, Siegfried Goerg, Andreas Greinacher, Marica Grossegesse, Sebastian Haller, Hans-Gert Heuft, Natalie Hofmann, Peter A. Horn, Claudia Houareau, Ilay Guelec, Carlos Luis Jimenez Klingberg, David Juhl, Monika Lindemann, Silke Martin, Hannelore K. Neuhauser, Andreas Nitsche, Julia Ohme, Sven Peine, Ulrich J. Sachs, Lars Schaade, Richard Schaefer, Heinrich Scheiblauer, Martin Schlaud, Michael Schmidt, Markus Umhau, Tanja Vollmer, Franz F. Wagner, Lothar H. Wieler, Hendrik Wilking, Malte Ziemann, Marlow Zimmermann, Matthias an der Heiden
Summary: SARS-CoV-2 serosurveillance is crucial for infection control measures and understanding underreporting. A study in Germany analyzed blood donor samples collected from April 2020 to April 2021, September 2021, and April/May 2022. The seroprevalence of SARS-CoV-2 increased over time, reaching 100% in April/May 2022. Neutralizing capacity was also observed in the majority of positive specimens. The study revealed low levels of underreporting, indicating an effective testing and notification system in Germany.
Article
Medical Informatics
Anatol-Fiete Naeher, Carina N. Vorisek, Sophie A. Klopfenstein, Moritz Lehne, Sylvia Thun, Shada Alsalamah, Sameer Pujari, Dominik Heider, Wolfgang Ahrens, Iris Pigeot, Georg Marckmann, Mirjam A. Jenny, Bernhard Y. Renard, Max von Kleist, Lothar H. Wieler, Felix Balzer, Linus Grabenhenrich
Summary: Substantial opportunities for global health intelligence and research can be achieved through the combined and optimised use of secondary data within data ecosystems. This includes data from emerging sources such as the internet, wearables, mobile phone apps, electronic health records, and genome sequencing. Guidance on available sources and approaches for processing secondary data, as well as criteria for assessing reusability, can support policy decision making and improve early detection and prevention of emerging health threats.
LANCET DIGITAL HEALTH
(2023)
Article
Public, Environmental & Occupational Health
Benjamin F. Maier, Annika H. Rose, Angelique Burdinski, Pascal Klamser, Hannelore Neuhauser, Ole Wichmann, Lars Schaade, Lothar H. Wieler, Dirk Brockmann
Summary: After the winter of 2021/2022, a considerable number of people in Germany have been infected with or vaccinated against the SARS-CoV-2 variant. However, due to under-reporting and unknown overlap between the vaccinated and recovered populations, the exact extent is difficult to estimate. This study provides estimates of the share of immunologically naive individuals in each age group for the German population by integrating infectious-disease modeling and vaccine uptake data. The study shows that a significant proportion of the population in Germany remains immunologically naive, highlighting the impact of the Omicron waves.
EPIDEMIOLOGY AND INFECTION
(2023)