Article
Food Science & Technology
Rongrong He, Qiuping Zhong, Weijun Chen, Ming Zhang, Jianfei Pei, Haiming Chen, Wenxue Chen
Summary: This study investigated the antibacterial mechanism of linalool and its application in chicken breast preservation using transcriptomics and proteomics. The results showed that linalool exerted antibacterial effect by damaging cellular structure, impairing energy metabolism, activating specific amino acid metabolism, and DNA repair. Additionally, linalool effectively prevented the growth of L. monocytogenes in chicken breast during storage without affecting sensory acceptability.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Microbiology
Damayanti Chakravarty, Gyan Sahukhal, Mark Arick II, Morgan L. Davis, Janet R. Donaldson
Summary: This study analyzed the differences in transcript levels produced by Listeria monocytogenes under conditions mimicking the gastrointestinal tract, revealing that anaerobic environments have an impact on gene transcription, particularly under acidic conditions. These findings provide comprehensive information on how L. monocytogenes responds to the gastrointestinal tract.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Paula Fernandez-Gomez, Jose F. Cobo-Diaz, Marcia Oliveira, Montserrat Gonzalez-Raurich, Avelino Alvarez-Ordonez, Miguel Prieto, James L. Walsh, Morten Sivertsvik, Estefania Noriega-Fernandez, Mercedes Lopez
Summary: Plasma-Activated Water (PAW) was generated from tap water and its antimicrobial activity against Listeria monocytogenes was evaluated. PAW generated at 36 W-30 min showed the most effective inactivation, reducing the cell count by 4.6 log in planktonic state. Although the antimicrobial activity in biofilms was lower, increasing the exposure time to 30 min resulted in a reduction of >4.5 log cycles. The mechanisms of action of PAW involved changes in carbon metabolism, virulence, and stress response genes.
Article
Food Science & Technology
Zhipeng Gao, Weiming Zhong, Ting Liu, Tianyu Zhao, Jiajing Guo
Summary: In this study, the response of Listeria monocytogenes exposed to linalool was explored using iTRAQ-based quantitative proteomics analysis to uncover the mode of action and drug targets of linalool against LM. A total of 445 differentially expressed proteins were identified, with 31 significantly enriched gene ontology functional categories and 60 significantly enriched biological pathways. The analysis suggested that cell membranes, cell walls, nucleoids, and ribosomes might be the targets of linalool against LM.
Article
Food Science & Technology
Jiajing Guo, Xiao Hu, Zhipeng Gao, Gaoyang Li, Fuhua Fu, Xuebo Shang, Zengenni Liang, Yang Shan
Summary: Research showed that Fingered Citron essential oil (FCEO) has strong anti-listeria activity, causing cell morphology changes, and L. monocytogenes exhibited various cell responses in its presence. Genome-wide transcriptome analysis revealed that L. monocytogenes adapt by enhancing motility, promoting escape mechanisms, increasing energy intake, and altering metallic cation uptake in response to FCEO exposure.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Biochemistry & Molecular Biology
Cleiton de Paula Soares, Michelle Zibetti Trada-Sfeir, Leonardo Araujo Terra, Jessica de Paula Ferreira, Carlos Magno Dos-Santos, Izamara Gesiele Bezerra de Oliveira, Jean Luiz Simoes Araujo, Carlos Henrique Salvino Gadelha Meneses, Emanuel Maltempi de Souza, Jose Ivo Baldani, Marcia Soares Vidal
Summary: This study provides a comprehensive analysis of the early transcriptional response of Gluconacetobacter diazotrophicus to iron limitations, revealing the regulatory mechanisms of genes related to iron homeostasis and identifying genes that are overexpressed under iron-limited conditions. Additionally, the study demonstrates that the fur protein of G. diazotrophicus can complement an E. coli fur mutant.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Sae-Hae Kim, Byeol-Hee Cho, Kwang Soon Kim, Yong-Suk Jang
Summary: C5a in the Peyer's patch enhances CD8(+) T cell responses by increasing C5/C5a levels. Single-cell RNA sequencing identifies dendritic cells expressing C5aR and lysozyme as key players in CD8(+) T cell immune induction.
Article
Food Science & Technology
Kye-Hwan Byun, Sang Ha Han, Min Woo Choi, Si Hong Park, Sang-Do Ha
Summary: The study found that sublethal concentrations of bactericidal antibiotics increased the mutation frequency in L. monocytogenes, facilitated the adaptation of the bacterium to food-associated stress, and led to an increase in its pathogenicity.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Environmental Sciences
Xiaxia Chen, Jie Wang, Zunqing Du, Qihang Shu, Zheng Zheng, Xingzhang Luo
Summary: Iron limitation inhibits the growth and photosynthetic activity of cyanobacteria. In this study, the effects of iron limitation on cell density, chlorophyll content, and photosynthetic activity in Microcystis aeruginosa were investigated. The results showed that iron deficiency significantly reduced cell density, chlorophyll-a content, and photosynthetic efficiency in M. aeruginosa. Transcriptome analysis revealed that genes involved in iron absorption and transport were up-regulated, while genes related to photosynthesis were down-regulated. These changes in gene expression may be a survival strategy for M. aeruginosa in an iron-deficient environment.
Article
Microbiology
Xifeng Zuo, Meilin Chen, Xinshuai Zhang, Ailing Guo, Si Cheng, Rong Zhang
Summary: Ralstonia insidiosa can survive in various environments and is harmful. It can form suspended aggregates with Listeria monocytogenes, increasing the risk of contamination. RNA sequencing and metabolomics were used to analyze the differentially expressed genes and metabolites involved in aggregate formation. The study found that under nutrient-poor conditions, certain genes and metabolites of R. insidiosa were significantly upregulated.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Arkadiusz Jozef Zakrzewski, Wioleta Chajecka-Wierzchowska, Anna Zadernowska
Summary: Ceviche is a traditional marinated raw fish dish commonly consumed in Pacific coastal countries. This study aimed to assess the viability of bacteria isolated from fish after the preparation of ceviche. The results showed that different strains of bacteria had varying survival rates and pathogenic potentials during the preparation process, and certain ingredients in ceviche exhibited antibacterial properties.
Article
Chemistry, Multidisciplinary
Yao Liu, Yiping Lu, Bo Ning, Xiaomin Su, Binru Yang, Haiqing Dong, Bo Yin, Zhiqing Pang, Shun Shen
Summary: In this study, an immunotherapy system based on red blood cell membrane encapsulated Listeria monacytagenes (Lmo@RBC) was developed to address the limitations of Lmo as a cancer vaccine. Lmo@RBC showed low systemic inflammatory response and enhanced accumulation in tumors, resulting in a strong and durable anti-tumor immune response with excellent therapeutic effects.
Article
Immunology
Robert M. Johnson, Adesola C. Olatunde, Lauren N. Woodie, Michael W. Greene, Elizabeth Hiltbold Schwartz
Summary: The study utilized a live pathogenic infection model to investigate the metabolic demands induced by infection, revealing that the greatest impact on metabolism occurred during the early immune response, coinciding with the greatest shift in innate cellular metabolism. Additionally, systemic metabolism returned to resting state during maximal T cell expansion, suggesting a relative metabolic homeostasis.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Food Science & Technology
Yuan Wang, Linjun Sun, Lili Hu, Zhen Wang, Xiang Wang, Qingli Dong
Summary: According to the study, the nutrition status affects the adhesion ability and biofilm formation of Listeria monocytogenes. Nutrient deficiency can enhance the adhesion and biofilm formation of the bacteria, and also lead to changes in the expression of critical biofilm-associated genes. These findings are important for understanding the spread and control of Listeria in food processing.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Microbiology
Banhong Liu, Xiaolin Zhu, Ning Zhang, Hui Zhang, Hongbo Li, Yonghua Qi, Haizhen Mo, Liangbin Hu
Summary: In this study, it was discovered that L. monocytogenes entered a viable but non-culturable (VBNC) state in the presence of FeSO4 and remained virulent to Caenorhabditis elegans. Transcriptomic analysis revealed increased gene transcription in the VBNC cells compared to dormant cells, specifically related to stress response and ribosome binding. The formation of the VBNC state in L. monocytogenes was found to be dependent on intracellular Fe2+ and other cascades related to ferroptosis.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Environmental Sciences
S. R. Milillo, B. Lungu, C. A. O'Bryan, S. E. Dowd, A. Muthaiyan, M. G. Johnson, S. C. Ricke
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
(2015)
Article
Dentistry, Oral Surgery & Medicine
M. Vujanac, V. S. Iyer, M. Sengupta, D. Ajdic
MOLECULAR ORAL MICROBIOLOGY
(2015)
Article
Integrative & Complementary Medicine
Arunachalam Muthaiyan, Debabrata Biswas, Philip G. Crandall, Brian J. Wilkinson, Steven C. Ricke
BMC COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2012)
Article
Agriculture, Multidisciplinary
Arunachalam Muthaiyan, Oswaldo Hernandez-Hernandez, F. Javier Moreno, Maria Luz Sanz, Steven C. Ricke
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2012)
Article
Biotechnology & Applied Microbiology
A. Muthaiyan, E. M. Martin, S. Natesan, P. G. Crandall, B. J. Wilkinson, S. C. Ricke
JOURNAL OF APPLIED MICROBIOLOGY
(2012)
Article
Environmental Sciences
Suwat Saengkerdsub, Jody M. Lingbeck, Heather H. Wilkinson, Corliss A. O'Bryan, Philip G. Crandall, Arunachalam Muthaiyan, Debabrata Biswas, Steven C. Ricke
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
(2013)
Article
Environmental Sciences
Suwat Saengkerdsub, Arunachalam Muthaiyan, Jody M. Lingbeck, Corliss A. O'Bryan, Philip G. Crandall, Steven C. Ricke
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART B-PESTICIDES FOOD CONTAMINANTS AND AGRICULTURAL WASTES
(2014)
Article
Biotechnology & Applied Microbiology
Debabrata Biswas, Nathan E. Wideman, Corliss A. O'Bryan, Arunachalam Muthaiyan, Jody M. Lingbeck, Philip G. Crandall, Steven C. Ricke
JOURNAL OF FOOD SAFETY
(2012)
Article
Energy & Fuels
Davidraj Johnravindar, Namasivayam Elangovan, Nellaiappan Olaganathan Gopal, Arunachalam Muthaiyan, Qiang Fei
Summary: Tapioca starch factories generate a large amount of wastewater and solid waste, with high levels of starchy lignocellulosic materials like cassava waste residue. Clostridium sp. AS3 strain showed promising biobutanol production capacity from CWR under optimized conditions, demonstrating potential for continuous production.
Article
Environmental Sciences
Thangavel Mohankumar, Haralur Shankaraiah Lalithamba, Krishnan Manigandan, Arunachalam Muthaiyan, Namasivayam Elangovan
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Shrawan Kumar, Anjali Kharb, Aman Vazirani, Rajinder Singh Chauhan, Goutam Pramanik, Mrittika Sengupta, Souradyuti Ghosh
Summary: Nucleic acid amplification technique (NAAT)-assisted detection is crucial for pathogen molecular diagnostics, but prior nucleic acid extraction is required. Traditional extraction methods limit the use of qPCR or PCR-like NAAT methods in resource-constrained settings.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Sayantan Tripathy, Tanvi Agarkar, Arunansu Talukdar, Mrittika Sengupta, Ashvani Kumar, Souradyuti Ghosh
Summary: This study reports a novel sample-to-answer workflow for detecting SARS-CoV-2 nucleic acid, which involves an indirect sequence-specific magneto-extraction method and electrochemical LAMP, demonstrating high sensitivity.
Review
Microbiology
Suraj Kumar Modi, Smriti Gaur, Mrittika Sengupta, Manu Smriti Singh
Summary: Antimicrobial Resistance (AMR) is a serious concern that causes 5 million deaths per year. Over the years, significant membrane changes due to broad spectrum drugs have resulted in bacterial resistance. This increasing drug resistance poses a global burden on healthcare and immediate action is needed. Nanoparticles (NP) have shown potential as therapeutic agents or carriers of antibiotics against multidrug resistant (MDR) pathogens. Surface functionalization of nanocarriers using aptamers, antibodies, etc. allows for targeted delivery and evasion of bacterial resistance mechanisms. Understanding the interactions between NP surface and bacterial membrane is crucial in determining the role of NP as therapeutic agents. Smart surface-functionalized nanocarriers can potentially act as targeted robots against resistant bacteria. Nanomedicine offers promising and alternative tools in overcoming AMR as bacterial strains continue to evolve into resistant versions.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Tanvi Agarkar, Sayantan Tripathy, Vipin Chawla, Mrittika Sengupta, Souradyuti Ghosh, Ashvani Kumar
Summary: This study developed an electrochemical biosensor based on transition metal oxide for highly sensitive detection of SARS-CoV-2 RNA. The optimized electrode design allowed for the detection of low copies of RNA, and the sensor demonstrated advantages such as affordability, decentralization, and high sensitivity. The biosensor successfully detected the target in various samples, including aqueous solution, samples spiked with excess human genomic DNA, and serum-spiked samples.
ANALYTICAL METHODS
(2022)
Article
Infectious Diseases
Dipti S. Hattangady, Atul K. Singh, Arun Muthaiyan, Radheshyam K. Jayaswal, John E. Gustafson, Alexander V. Ulanov, Zhong Li, Brian J. Wilkinson, Richard F. Pfeltz