Article
Biotechnology & Applied Microbiology
Shilin Wu, Xueming Xu, Na Yang, Yamei Jin, Zhengyu Jin, Zhengjun Xie
Summary: The study found that induced electric fields can deactivate E.coli by destroying the cell membrane, denaturing intracellular enzymes, and rupturing the cell membrane, making it a potential technique for liquid food processing.
Article
Food Science & Technology
Jong-Seong Lim, Jae-Won Ha
Summary: This study found that acid-adapted E. coli O157:H7 exhibited higher X-ray resistance compared to non-acid-adapted cells in apple juice, while acid-adapted and non-acid-adapted Salmonella Typhimurium cells showed similar resistance. The quality attributes of apple juice were not significantly affected by X-ray irradiation, suggesting the importance of considering inactivation of acid-adapted E. coli O157:H7 cells in the application of X-ray sterilization in apple juice processing.
Article
Acoustics
Jiao Li, Donghong Liu, Tian Ding
Summary: The study investigated the gene regulation at the transcription level in Escherichia coli O157:H7 after ultrasonic stimulation, revealing significant differential expressions of 1217 genes, including up-regulated and down-regulated genes related to crucial biomolecular processes. The most enriched pathways were analyzed in carbohydrate metabolism, energy metabolism, membrane transport, signal transduction, transcription, and translation categories. Additionally, the study found that the citrate cycle genes were down-regulated, affecting carbohydrate decomposition and energy production under ultrasonic stress.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Tao Yang, Zhenghui Wang, Yang Song, Xinyan Yang, Sihan Chen, Shiqian Fu, Xue Qin, Wei Zhang, Chaoxin Man, Yujun Jiang
Summary: A smartphone-based colorimetric aptasensor was developed for the detection of E. coli O157:H7 in milk, showing good reproducibility with no cross-reaction for other bacteria. The sensor could detect a concentration of 8.43 x 10^3 cfu/mL of E. coli O157:H7 in pure culture and 5.24 x 10^2 cfu/mL in artificially contaminated milk after 1 hour of incubation.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Chemistry, Analytical
Zhihua Li, Xue Zhang, Haishan Qi, Xiaowei Huang, Jiyong Shi, Xiaobo Zou
Summary: A renewable electrochemical biosensor based on mussel-inspired adhesive protein was developed for the detection of Escherichia coli O157:H7. The biosensor showed excellent repeatability and sensitivity, with a wide linear range and a low detection limit. It demonstrated good performance in detecting E. coli O157:H7 in seafood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biotechnology & Applied Microbiology
Yu Cheng, Su Zhang, Chen Zhang, Xiaoyu Mi, Wendong Zhang, Longfeng Wang, Wenzheng Liu, Yun Jiang
Summary: This study investigated the interaction between E. coli O157:H7 and Pseudomonas, and their impact on meat processing and preservation. The results showed that their coexistence had a negative effect on the growth fitness of E. coli. Additionally, E. coli was observed to stably coexist at a low cellular abundance, suggesting the presence of adaptive mechanisms that require further investigation.
Article
Biotechnology & Applied Microbiology
Eun-Jung Lee, Soo-Hwan Kim, Sang-Hyun Park
Summary: The objective of this study was to evaluate the survival and cell injury of Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes in apple puree after high hydrostatic pressure (HHP) treatment. The results showed that increasing pressure level and lowering pH of apple puree led to larger reductions in microbial levels. E. coli O157:H7 exhibited higher resistance compared to the other two pathogens.
Article
Food Science & Technology
Cuong Huu Nguyen, Rohan Tikekar, Nitin Nitin
Summary: This study evaluated the synergistic combination of high-frequency ultrasound and the food-grade antioxidant propyl gallate for non-thermal processing of liquids. The results demonstrated significant and rapid inactivation of inoculated model Gram positive and Gram negative bacteria in apple juice using this combination, through enhanced membrane damage and oxidative stress induction in bacteria. This highlights the potential of this non-thermal processing method for liquid beverage processing.
INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES
(2022)
Article
Chemistry, Multidisciplinary
Sarah H. Klass, Laura E. Sofen, Zachary F. Hallberg, Tahoe A. Fiala, Alexandra Ramsey, Nicholas S. Dolan, Matthew B. Francis, Ariel L. Furst
Summary: Pathogenic E. coli strains are a significant threat to public health, causing both foodborne illnesses and urinary tract infections. By using a rapid bioconjugation reaction and electrochemical impedance spectroscopy, we can selectively capture and accurately quantify these pathogenic microbes on a disposable gold electrode.
CHEMICAL COMMUNICATIONS
(2021)
Article
Veterinary Sciences
Delphine Bibbal, Philippe Ruiz, Panagiotis Sapountzis, Christine Mazuy-Cruchaudet, Estelle Loukiadis, Frederic Auvray, Evelyne Forano, Hubert Brugere
Summary: This study aimed to identify farms with persistent circulation of EHEC O157:H7 in cattle. The results showed that persistent circulation may be due to the presence of a few predominant strains or repeated exposure of cattle to various strains. In addition, differences were found in the fecal microbial communities of shedders and non-shedders, with different bacterial indicators identified.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Gabor Grezal, Reka Spohn, Orsolya Mehi, Anett Dunai, Viktoria Lazar, Balazs Balint, Istvan Nagy, Csaba Pal, Balazs Papp
Summary: The evolution of antibiotic resistance in bacteria often has negative effects on microbial growth, virulence, and susceptibility to other antimicrobial agents. The underlying molecular mechanisms of these trade-offs are poorly understood. In this study, we show that clinically relevant resistance mutations in Escherichia coli lead to consistent and stereotypic changes in a large fraction of the transcriptome. These changes alter various physiological processes and enhance sensitivity to antimicrobial peptides, suggesting a common global mechanism for resistance evolution.
MOLECULAR BIOLOGY AND EVOLUTION
(2023)
Article
Food Science & Technology
Kai Dong, Yuwan Li, Sijia Luo, Fan Zhang, Hanxu Pan, Liang Zhao, Yongtao Wang, Xiaojun Liao
Summary: The study analyzed the sterilizing effects of different HHP-boosting processes on Escherichia coli O157:H7, finding that linear and fast boosting had the most significant bactericidal effects. The proteins interacting with GroEL under different boost modes and rates were involved in 42 metabolic pathways, particularly ribosome, ABC transporter, oxidative phosphorylation, sulfate assimilation, and cysteine biosynthesis. Key interacting proteins NuoB, NuoF, CysD, ZnuC, and RplX were identified and their interactions with GroEL were verified in vivo.
Article
Immunology
Juliana Gonzalez, Jimena Soledad Cadona, Claudio Marcelo Zotta, Silvina Lavayen, Roberto Vidal, Nora Lia Padola, Andrea Mariel Sanso, Ana Victoria Bustamante
Summary: This study aimed to compare the genetic diversity of VTEC O157:H7 strains isolated from human disease cases in Argentina and Chile. The results showed that these strains mainly belonged to lineage I/II, associated with hypervirulent strains, and were categorized into the phylogroup E. Additionally, genetic diversity was observed among Argentinean and Chilean strains, particularly in relation to putative virulence determinants and nle profiles.
MICROBES AND INFECTION
(2022)
Article
Veterinary Sciences
Aklilu Feleke Haile, Silvia Alonso, Nega Berhe, Tizeta Bekele Atoma, Prosper N. Boyaka, Delia Grace
Summary: This study estimated the prevalence and antimicrobial resistance of Escherichia coli O157:H7 in raw beef sold in butcher shops in Addis Ababa, Ethiopia. Out of the 384 samples examined, 3.64% carried E. coli O157:H7, with 57.14% of the isolates showing resistance to three or more antimicrobial agents. Thus, stricter monitoring of antimicrobial use and further studies on contamination sources and risk mitigation strategies are needed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Chemistry, Analytical
Manman Du, Jinbiao Ma, Zhiwei Zhang, Guangzu Wu, Jianguo Wu, Hao Wang, Xinwu Xie, Can Wang
Summary: This study developed a rGO-FET biosensor for the rapid and sensitive detection of pathogenic microorganisms. The biosensor demonstrated good specificity, a wide linear range, and the ability to directly detect real samples without pretreatment.
ANALYTICA CHIMICA ACTA
(2023)
Review
Biotechnology & Applied Microbiology
Teresa M. Bergholz, Manoj K. Shah, Laurel S. Burall, Mira Rakic-Martinez, Atin R. Datta
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Manoj K. Shah, Rhodel Bradshaw, Esmond Nyarko, Eric T. Handy, Cheryl East, Patricia D. Millner, Teresa M. Bergholz, Manan Sharma
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Manoj K. Shah, Rhodel Bradshaw, Esmond Nyarko, Patricia D. Millner, Deborah Neher, Thomas Weicht, Teresa M. Bergholz, Manan Sharma
JOURNAL OF FOOD PROTECTION
(2019)
Article
Microbiology
Maria X. Cardenas-Alvarez, Megan K. Townsend Ramsett, Sahar Malekmohammadi, Teresa M. Bergholz
JOURNAL OF MEDICAL MICROBIOLOGY
(2019)
Article
Food Science & Technology
Deepti Tyagi, Autumn L. Kraft, Sara Levadney Smith, Sherry E. Roof, Julie S. Sherwood, Martin Wiedmann, Teresa M. Bergholz
Article
Biotechnology & Applied Microbiology
M. K. Shah, T. M. Bergholz
JOURNAL OF APPLIED MICROBIOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Jane Snelling, Sahar Malekmohammadi, Teresa M. Bergholz, Jae Ohm, Senay Simsek
JOURNAL OF FOOD PROTECTION
(2020)
Article
Chemistry, Applied
Senay Simsek, Jane Snelling, Sahar Malekmohammadi, Teresa M. Bergholz
Summary: The study showed that vacuum steam treatment is effective in eliminating pathogens on wheat without compromising flour quality. Bread quality is significantly impacted by high temperature treatments, while cake and cookie quality remains relatively unaffected.
Article
Biotechnology & Applied Microbiology
Sahar Malekmohammadi, Manoj K. Shah, Megan K. Townsend Ramsett, Teresa M. Bergholz
Article
Green & Sustainable Science & Technology
Jamileh Shojaeiarani, Dilpreet S. Bajwa, Nicole M. Stark, Teresa M. Bergholz, Autumn L. Kraft
SUSTAINABLE MATERIALS AND TECHNOLOGIES
(2020)
Article
Biotechnology & Applied Microbiology
Jessica R. Lauer, Senay Simsek, Teresa M. Bergholz
Summary: This study evaluates the long-term survival of enteric pathogens on wheat grain, showing varying survival capabilities between different strains of Salmonella and EHEC. It highlights the importance of considering food safety concerns when it comes to the long shelf life of wheat grain and its products in a low-moisture environment.
JOURNAL OF FOOD PROTECTION
(2021)
Article
Biotechnology & Applied Microbiology
Yawei Lin, Senay Simsek, Teresa M. Bergholz
Summary: The use of chlorinated water in the wheat milling industry can effectively reduce the numbers of enteric pathogens on wheat kernels without affecting flour quality and gluten functionality.
JOURNAL OF FOOD PROTECTION
(2022)
Article
Microbiology
Maria X. Cardenas-Alvarez, Hui Zeng, Brett T. Webb, Rinosh Mani, Marina Munoz, Teresa M. Bergholz
Summary: Ruminants are reservoirs for Listeria monocytogenes and can carry the pathogen asymptomatically or develop clinical neurologic or fetal infections. This study characterized 73 L. monocytogenes isolates from ruminant listeriosis cases in the US and identified different lineages associated with specific infection types. The presence of virulence factors, stress-associated genes, and mobile genetic elements was also evaluated.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Maria X. Cardenas-Alvarez, Daniel Restrepo-Montoya, Teresa M. Bergholz
Summary: Heterogeneity in virulence potential of L. monocytogenes subgroups is associated with advantageous genetic elements. Gene mutations are related to attenuated phenotypes, while the presence of pathogenicity islands (PI) is associated with hypervirulent or stress-resistant clones.
Article
Biotechnology & Applied Microbiology
Yawei Lin, Senay Simsek, Teresa M. Bergholz
Summary: This study aimed to investigate the behavior of Salmonella and STEC during wheat processing. The results showed that the bacterial population inoculated on the surface of wheat kernels did not significantly change during tempering, and the localization of bacteria on kernels remained unchanged after tempering. The study suggests that disinfection of wheat grains prior to milling could reduce food safety concerns.