Article
Agriculture, Multidisciplinary
So-Rim Kim, Minseok Cha, Taeok Kim, Sihoon Song, Hye Jee Kang, Younghoon Jung, Jeong-Yong Cho, Sang Hyun Moh, Soo-Jung Kim
Summary: The purpose of this study was to develop yeast strains for sustainable and economical production of Mycosporine-like amino acids (MAAs), particularly shinorine. By introducing genes involved in the MAA biosynthetic pathway and utilizing combinatorial gene expression, the engineered Saccharomyces cerevisiae strain successfully produced high concentrations of shinorine in fed-batch fermentation.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Agricultural Engineering
Shaozheng Li, Changsheng Su, Mudannan Fang, Di Cai, Li Deng, Fang Wang, Junfeng Liu
Summary: This study investigated the feasibility of using corn stover hydrolysate (CSH) as a carbon source for the production of palmitoleic acid (POA) by engineered S. cerevisiae. Although the growth of yeast was inhibited by CSH to some extent, the POA production with CSH was slightly higher than that with pure glucose. The C/N ratio of 120 and the addition of 1 g/L lysine increased the POA titer. Under optimized conditions, a high POA content of 57.5% (v/v) and a highest POA titer of 6.56 g/L were achieved.
BIORESOURCE TECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Yuan Zhu, Jianxiu Li, Longyun Peng, Lijun Meng, Mengxue Diao, Shuiyuan Jiang, Jianbin Li, Nengzhong Xie
Summary: In this study, a highly efficient yeast cell factory was constructed for the production of high-value-added PPD and its derivatives. By modulating gene expression, introducing dynamic control, and utilizing inexpensive substrates, large-scale PPD production was achieved, setting new records.
MICROBIAL CELL FACTORIES
(2022)
Article
Agriculture, Multidisciplinary
Guangjuan Luo, Ying Lin, Shuting Chen, Ruiming Xiao, Jiaxin Zhang, Cheng Li, Anthony J. Sinskey, Lei Ye, Shuli Liang
Summary: In this study, a high-yield patchoulol-producing strain P6H53 was constructed through systematic engineering studies on Komagataella phaffii. It achieved the highest production to date in a 5 L fermenter, laying a good foundation for the development of K. phaffii as a promising microbial cell for the synthesis of patchoulol and other sesquiterpenes.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Tae Yeob Kim, Haeseong Park, Sun-Ki Kim, Soo-Jung Kim, Yong-Cheol Park
Summary: Metabolic engineering strategies were employed to produce (-)-alpha-bisabolol in Saccharomyces cerevisiae, resulting in increased production through optimized gene expression, intensified mevalonate pathway, and enhanced acetyl-CoA pool. The engineered strain achieved significantly higher yield and productivity of (-)-alpha-bisabolol in fed-batch fermentation compared to the initial strain in batch culture, indicating the potential for sustainable production of sesquiterpenes in engineered S. cerevisiae.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Xiaomin Deng, Bin Shi, Ziling Ye, Man Huang, Rong Chen, Yousheng Cai, Zhaolin Kuang, Xiang Sun, Guangkai Bian, Zixin Deng, Tiangang Liu
Summary: This study systematically screened and identified two sesquiterpenoid biosynthesis genes from Holy basil in India, successfully recognizing (-)-eremophilene and achieving high-yield bioproduction through a yeast system, while also confirming its insect-repelling effect.
METABOLIC ENGINEERING
(2022)
Article
Agricultural Engineering
Xiang Xiu, Yi Sun, Yaokang Wu, Ke Jin, Lisha Qu, Yanfeng Liu, Jianghua Li, Guocheng Du, Xueqin Lv, Long Liu
Summary: This study achieved highly efficient 7-DHC synthesis by remodeling the complicated sterol synthesis modules. By overexpressing multiple genes and a transcription factor mutant, as well as applying CRISPR-mediated activation and repression systems, the titer of 7-DHC was successfully increased. Furthermore, by compartmentalizing enzymes and enlarging the endoplasmic reticulum lumen, the titer of 7-DHC was further enhanced, reaching the highest reported level so far.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Jiajia Liu, Chang Chen, Xiukun Wan, Ge Yao, Shaoheng Bao, Fuli Wang, Kang Wang, Tianyu Song, Penggang Han, Hui Jiang
Summary: The study successfully achieved high production of (-)-germacrene D in Saccharomyces cerevisiae through terpene synthase mining and metabolic engineering, demonstrating the importance of microbial overproduction of high-value compounds.
MICROBIAL CELL FACTORIES
(2022)
Article
Biochemistry & Molecular Biology
Masahiko Sugimura, Taisuke Seike, Nobuyuki Okahashi, Yoshihiro Izumi, Takeshi Bamba, Jun Ishii, Fumio Matsuda
Summary: This study found that deletion of RIM15 and activation of the pyruvate-consuming pathway can increase the production and yield of glycolytic bioalcohol 2,3-BDO in metabolically engineered S. cerevisiae strains.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Agriculture, Multidisciplinary
Junhua Wang, Linghuan Zhu, Youran Li, Sha Xu, Wei Jiang, Chaojuan Liang, Yakun Fang, Alex Chu, Liang Zhang, Zhongyang Ding, Guiyang Shi
Summary: By using a GGPP synthase with stronger catalytic ability and increasing the metabolic flux of the MVA pathway and the supply of IPP, the production of GGOH in Saccharomyces cerevisiae was significantly enhanced. These strategies can also be applied to enhance the production of other terpenoids in the future.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Biochemical Research Methods
Pingping Wang, Zhenjun Fan, Wenping Wei, Chengshuai Yang, Yan Wang, Xiao Shen, Xing Yan, Zhihua Zhou
Summary: Osthole, a compound found in traditional Chinese medicine, has been found to possess potential bronchodilator activity by inhibiting phosphodiesterase 4D, making it a novel treatment for asthma that does not target β2-adrenoceptors.
ACS SYNTHETIC BIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Junhua Wang, Youran Li, Wei Jiang, Jinyuan Hu, Zhenghua Gu, Sha Xu, Liang Zhang, Zhongyang Ding, Wei Chen, Guiyang Shi
Summary: Optimization of supply and conversion efficiency of geranylgeranyldiphosphate (GGPP) is crucial for increasing geranylgeraniol (GGOH) production in Saccharomyces cerevisiae. By overexpressing all genes of the mevalonate (MVA) pathway, a strain producing 26.92 +/- 1.59 mg/g of squalene was constructed, and further engineering led to a GGOH titer of 597.12 mg/L at the shake flask level. Additional expression of PaGGPPs-ERG20 and PaGGPPs-DPP1, along with downregulation of ERG9, increased the GGOH titer to 1221.96 mg/L. Introduction of a NADH HMG-CoA reductase from Silicibacter pomeroyi (SpHMGR) further improved GGOH production to 1271.14 mg/L. Fed-batch fermentation optimization in a 5 L bioreactor resulted in a GGOH titer of 6.33 g/L, 24.9% higher than the previous report. This study has significant implications for the development of S. cerevisiae cell factories for diterpenoid and tetraterpenoid production.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Ye-Gi Lee, Jin-Myung Bae, Soo-Jung Kim
Summary: In this study, Saccharomyces cerevisiae was engineered to produce enantiopure meso-2,3-BDO and achieved high productivity using cassava hydrolysates. The researchers also improved the titer and productivity of meso-2,3-BDO by resolving C-2-auxotrophy. These findings have important implications for the commercial production of enantiopure meso-2,3-BDO.
JOURNAL OF BIOTECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Dielle Pierotti Procopio, Emanuele Kendrick, Rosana Goldbeck, Andre Ricardo de Lima Damasio, Telma Teixeira Franco, David J. Leak, Yong-Su Jin, Thiago Olitta Basso
Summary: The engineering of xylo-oligosaccharide-consuming Saccharomyces cerevisiae strains is a promising approach for the effective utilization of lignocellulosic biomass and development of economic industrial fermentation processes.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Pingping Zhou, Xiuqi Zhou, Doudou Yuan, Xin Fang, Xueqing Pang, Ke Yuan, Aipeng Li, Xiqing Wang
Summary: In this study, the entrance of the substrate-binding pocket of linalool synthase was engineered to increase its catalytic efficiency, resulting in increased linalool production. Additionally, the use of cytoplasm and peroxisomes boosted linalool synthesis in Saccharomyces cerevisiae, achieving the highest linalool production in yeast to date.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)