Article
Food Science & Technology
Monica Campas, Sandra Leonardo, Ainoa Ferre-Gode, Asif A. Chowdhury, Anna Toldra, Karl B. Andree, Ana Roque
Summary: Vibrio parahaemolyticus is a dominant cause of foodborne infections and can accumulate in shellfish. A duplex electrochemical biosensor was developed to detect the tdh and trh genes of V. parahaemolyticus in oysters, providing a quick and specific method for monitoring this pathogen.
Article
Chemistry, Analytical
Ritong Sun, Jiao Chen, Yingeng Wang, Zheng Zhang, Yong Li, Fengmei Li, Cuiping Ma, Qingxia Han, Yanjing Shi
Summary: A highly sensitive and specific real-time narrow thermal-cycling amplification detection method based on accelerated strand exchange amplification (ASEA) was developed for the detection of Vibrio parahaemolyticus. The method can detect low concentrations of Vibrio parahaemolyticus in cultured samples and artificially spiked scallop meat within a short time. It is a useful tool for rapid detection of Vibrio parahaemolyticus in seafood samples.
ANALYTICAL METHODS
(2023)
Article
Engineering, Environmental
Zaafrane Sami, Maatouk Kaouthar, Cherif Nadia, Ben Mansour Hedi
Summary: This study investigated the impact of sunlight and salinities on the survival of Vibrio parahaemolyticus strains. The results showed that sunlight exposure caused the strains to become non-culturable but viable. The tdh positive strains were more culturable than the non-virulent or trh positive strain. The survival time was longer at certain salinities and the non-pathogenic strain survived longer than the virulent ones. Resuscitation experiments did not revive the non-culturable bacteria. Microscope observations showed that the strains remained motile after losing their culturability.
WATER ENVIRONMENT RESEARCH
(2022)
Article
Microbiology
Zhuosheng Liu, Chao Liao, Luxin Wang
Summary: In this study, the population dynamics of Vibrio parahaemolyticus (Vp) strains with different virulence genes were characterized by inoculating them into seawater at temperatures mimicking different seasons. The transcriptomic responses of the strains containing the thermostable direct hemolysin (tdh+) gene and the tdh-related hemolysin (trh+) gene were investigated. The results showed that the tdh+ strain had a higher growth rate than the trh+ strain at 30 degrees C, while there was no difference between the two strains at 10 degrees C. The RNA sequencing results revealed specific metabolic changes in the trh+ strain at 30 degrees C and downregulation of histidine metabolism and biofilm formation pathways in both strains at 10 degrees C. The expression of virulence-associated genes did not differ significantly between the two temperatures.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
Guoxia Zheng, Qian Gao, Youwei Jiang, Ling Lu, Jianfeng Li, Xingcai Zhang, Hongyu Zhao, Panpan Fan, Yutong Cui, Furong Gu, Yunhua Wang
Summary: By combining digital microfluidic (DMF) with loop-mediated isothermal amplification (LAMP), an automated instrument-compact DMF-LAMP device was developed for rapid and sensitive detection of Vibrio parahaemolyticus. Proper mixing was found to enhance the amplification reaction rate, shorten the amplification time, and improve detection sensitivity of the device. The device showed high sensitivity with a detection limit of <0.23 x 10(3) CFU/g, making it a promising sample-to-answer system for quick and sensitive analysis.
ANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Xinrui Lv, Weiwei Cao, Huang Zhang, Yilin Zhang, Lei Shi, Lei Ye
Summary: A system combining chemically enhanced CRISPR and RAA technologies was established for detecting V. parahaemolyticus in seafood, showing rapid, accurate detection with no cross-reactivity.
Article
Microbiology
Abu Baker Siddique, M. Moniruzzaman, Sobur Ali, Md Nayem Dewan, Mohammad Rafiqul Islam, Md Shafiqul Islam, Mohammed Badrul Amin, Dinesh Mondal, Anowar Khasru Parvez, Zahid Hayat Mahmud
Summary: The study investigated the incidence and virulence potential of V. parahaemolyticus isolates in aquaculture farms in Bangladesh, revealing high antibiotic resistance and O8:KUT as the predominant serotype. Environmental factors such as temperature and salinity were significantly correlated with V. parahaemolyticus contamination. The genetic variation and relatedness among pathogenic isolates were determined using ERIC-PCR.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Chemistry, Analytical
Yong Li, Shuo Lin, Yuxin Xue, Qianyue Jia, Ye Wang, Yingqiu Xie, Chao Shi, Cuiping Ma
Summary: BNNPs have been proven to significantly improve the specificity and sensitivity of PCR and LAMP amplification reactions, expanding the application range of nucleic acid detection and increasing detection sensitivity.
ANALYTICA CHIMICA ACTA
(2023)
Article
Microbiology
Cristina Bacian, Cristobal Verdugo, Katherine Garcia, Josu Perez-Larruscain, Ignacio de Blas, Viviana Cachicas, Carmen Lopez-Joven
Summary: Vibrio parahaemolyticus is the leading cause of seafood-associated bacterial gastroenteritis worldwide. This study determined the prevalence of V. parahaemolyticus in mussels from two natural growing areas in Chile, finding higher detection rates in the Valdivia River Estuary compared to the Reloncavi Fjord, with significant influence from seasonal water temperature and area factors.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Prasenjit Paria, Bijay Kumar Behera, Pradeep Kumar Das Mohapatra, Pranaya Kumar Parida
Summary: The study revealed that Vibrio parahaemolyticus strains positive for tdh and trh exhibit high pathogenicity and resistance to antibiotics, causing severe pathological changes in Indian Major Carp, Labeo rohita. Proper management strategies are needed to prevent potential disease outbreaks.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Food Science & Technology
Zenghui Gao, Heer Ding, Ke Li, Qiang Zhang, Xubo Zhao, Bin Liu, Tianli Yue
Summary: Despite becoming the most common pathogen in seafood, the changes in virulence of Vibrio parahaemolyticus are often overlooked during seafood processing. Using frozen Penaeus chinensis as examples, we investigated the potential risks and determined the most virulent factors of V. parahaemolyticus, such as bacterial quantities, viable but nonculturable (VBNC) status, toxins TDH and TRH, and virulence genes tdh and trh. The bacterial quantities were significantly reduced during drain and sprinkling phases due to different factors. Washing was the main reason for bacterial reduction at the drain phase, while the strain entering VBNC state was another reason at the sprinkling phase. The results showed that the strains did not die during the freezing process, but became VBNC cells that still produced and accumulated toxins, especially the most virulent factor TDH.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Public, Environmental & Occupational Health
Xiaolan Huang, Fei Xiao, Nan Jia, Chunrong Sun, Jin Fu, Zheng Xu, Xiaodai Cui, Hui Huang, Dong Qu, Juan Zhou, Yi Wang
Summary: The ongoing outbreak of monkeypox, caused by monkeypox virus (MPXV), requires a rapid and sensitive MPXV detection method. In this study, a diagnostic test called MPX-LAMP-LFB was developed based on loop-mediated isothermal amplification (LAMP) and nanoparticle-based lateral flow biosensor (LFB) for the diagnosis of MPXV infection. The MPX-LAMP-LFB assay can specifically detect MPXV strains without cross-reaction with non-MPXV pathogens and has a low sensitivity of 5 copies/µl of plasmid template and 12.5 copies/µl of pseudovirus in human blood samples. The entire process of the assay can be completed in about 1 hour, making it a useful tool for rapid and reliable diagnosis of MPXV infection.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Food Science & Technology
Xinran Xiang, Enjie Diao, Yuting Shang, Minghui Song, Yinglong He
Summary: With the rapid development of logistics, there is an urgent need to develop effective detection strategies to ensure food safety, as a growing number of pathogenic microorganisms spread worldwide using food as a carrier. This study developed a high-fidelity target-based microfluidic identification (HFTMI) strategy by combining novel markers identified by pan-genome analysis and a digital recombinase-aided amplification (RAA) detection method. The HFTMI system showed improved performance compared to qPCR, providing more reliable guidance for food safety testing.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Environmental Sciences
Xiaotian Han, Ting Zhao, Tian Yan, Rencheng Yu
Summary: A LAMP method coupled with a LFD was developed for detecting harmful algae Karenia mikimotoi, showing high sensitivity and specificity, indicating a promising approach for detecting and monitoring harmful algal blooms.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Food Science & Technology
Qian Yang, Wei Guo, Yi Liu, Yunzhe Zhang, Ruoyang Ming, Yaowu Yuan, Jianxin Tan, Wei Zhang
Summary: A novel SPIA technique modified with SYBR Green II was developed for visual detection of V. parahaemolyticus in seafood samples, achieving high sensitivity, specificity, and accuracy. The method showed a LOD of 5.7 CFU/mL in pure culture and 42 CFU/g in raw oysters by visual fluorescence, demonstrating its potential as a rapid and specific tool for routine surveillance of food-borne pathogens.
FOOD ANALYTICAL METHODS
(2021)
Article
Immunology
Yen-Hung Liu, Wataru Yamazaki, Yu-Tsung Huang, Chun-Hsing Liao, Wang-Hui Sheng, Po-Ren Hsueh
JOURNAL OF MICROBIOLOGY IMMUNOLOGY AND INFECTION
(2019)
Article
Infectious Diseases
Wataru Yamazaki, Ryohei Makino, Konomu Nagao, Hirohisa Mekata, Kenji Tsukamoto
TRANSBOUNDARY AND EMERGING DISEASES
(2019)
Article
Biotechnology & Applied Microbiology
Islam Ibrahim Sabike, Wataru Yamazaki
JOURNAL OF FOOD PROTECTION
(2019)
Article
Biotechnology & Applied Microbiology
N. Yashiki, Y. Yamazaki, M. Subangkit, T. Okabayashi, W. Yamazaki, Y. Goto
LETTERS IN APPLIED MICROBIOLOGY
(2019)
Article
Environmental Sciences
Ryohei Makino, Yasuko Yamazaki, Konomu Nagao, Francis Victor Apego, Hirohisa Mekata, Wataru Yamazaki
FOOD AND ENVIRONMENTAL VIROLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Y. Yamazaki, U. Thongchankaew-Seo, K. Nagao, H. Mekata, W. Yamazaki
LETTERS IN APPLIED MICROBIOLOGY
(2020)
Article
Agriculture, Dairy & Animal Science
Thi Ngan Mai, Wataru Yamazaki, Thanh Phong Bui, Van Giap Nguyen, Thi My Le Huynh, Shuya Mitoma, Hala El Daous, Emmanuel Kabali, Junzo Norimine, Satoshi Sekiguchi
TROPICAL ANIMAL HEALTH AND PRODUCTION
(2020)
Article
Virology
Wataru Yamazaki, Yasufumi Matsumura, Uraiwan Thongchankaew-Seo, Yasuko Yamazaki, Miki Nagao
Summary: The study has successfully developed a simple and rapid method for extracting RNA from saliva and conducting COVID-19 testing, which can be used in field settings to potentially reduce the risk of infection.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Infectious Diseases
Emmanuel Kabali, Girja Shanker Pandey, Musso Munyeme, Penjaninge Kapila, Andrew Nalishuwa Mukubesa, Joseph Ndebe, John Bwalya Muma, Charles Mubita, Walter Muleya, Elizabeth Muligisa Muonga, Shuya Mitoma, Bernard Mudenda Hang'ombe, Anuwat Wiratsudakul, Mai Thi Ngan, Eslam Elhanafy, Hala El Daous, Nguyen Thi Huyen, Wataru Yamazaki, Tamaki Okabayashi, Maiku Abe, Junzo Norimine, Satoshi Sekiguchi
Summary: This cross-sectional study in Zambia discovered the transmission of antimicrobial resistance among Escherichia coli and other enterobacteria isolated from healthy wildlife and livestock cohabiting in a game ranch near Lusaka. The study highlights the need for further investigation on the role of wildlife in the development and transmission of antimicrobial resistance, a global concern.
Article
Multidisciplinary Sciences
Yasuko Yamazaki, Uraiwan Thongchankaew-Seo, Wataru Yamazaki
Summary: The study investigated the presence of SARS-CoV-2 in cultivated oysters sold in Japan and evaluated the risk of food-borne transmission. The results indicated that consumption of oysters cultivated in coastal areas may not be a risk factor for SARS-CoV-2 outbreaks, suggesting that well-developed sewage treatment facilities contribute to preventing viral contamination.
Article
Veterinary Sciences
Mai Thi Ngan, Huynh Thi My Le, Vu Ngoc Dang, Trinh Thi Bich Ngoc, Le Van Phan, Nguyen Thi Hoa, Truong Quang Lam, Nguyen Thi Lan, Kosuke Notsu, Satoshi Sekiguchi, Yasuko Yamazaki, Wataru Yamazaki
Summary: The lack of practical testing methods for African swine fever (ASF) has hindered early detection and early containment of the pandemic. In this study, a rapid and sensitive point-of-care test (POCT) was developed to detect ASF using swine whole blood samples. The POCT enabled crude DNA to be extracted from swine whole blood samples within 10 min at low cost and with ease. Compared to conventional real-time PCR detection, the POCT showed slightly lower sensitivity but comparable diagnostic accuracy. It is expected that this POCT will facilitate early diagnosis and containment of ASF.
VETERINARY MEDICINE AND SCIENCE
(2023)
Article
Infectious Diseases
Yasufumi Matsumura, Wataru Yamazaki, Taro Noguchi, Masaki Yamamoto, Miki Nagao
Summary: Direct detection tests for SARS-CoV-2 in respiratory samples show promising potential for rapid diagnosis of COVID-19. However, there are large differences in the performance of different assays, and laboratories need to consider these characteristics before implementing them.
JOURNAL OF CLINICAL VIROLOGY PLUS
(2023)
Article
Veterinary Sciences
Angela Patricia B. Chua, Remil L. Galay, Tetsuya Tanaka, Wataru Yamazaki
VETERINARY SCIENCES
(2020)
Article
Veterinary Sciences
Konomu Nagao, Ryohei Makino, Francis Victor Apego, Hirohisa Mekata, Wataru Yamazaki
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2019)
Meeting Abstract
Public, Environmental & Occupational Health
Oscar Escalante-Maldonado, Maria Paquita Garcia, Cesar Cabezas, Ronnie Gustavo Gavilan, Wataru Yamazaki
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2018)
