Article
Agricultural Engineering
Sumao Zhao, Wei Xu, Wenli Zhang, Hao Wu, Cuie Guang, Wanmeng Mu
Summary: The study successfully cloned and expressed methyl parathion hydrolase from Azohydromonas australica in Escherichia coli, showing excellent enzymatic activity at high pH and temperature. The addition of Mn2+ significantly enhanced enzyme activity and improved thermal stability. The enzyme effectively degraded various organophosphorus pesticides in minutes, indicating great potential for bioremediation applications.
BIORESOURCE TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Minwen Sun, Wei Xu, Wenli Zhang, Cuie Guang, Wanmeng Mu
Summary: In this study, the researchers prepared a metal-organic framework (MOF) MIL-88A and used it as a carrier for immobilizing the methyl parathion hydrolase from Azohydromonas australica (Azau-MPH). The immobilized Azau-MPH showed higher activity and better storage stability compared to the free enzyme. It also exhibited a high reuse efficiency and sensitivity for detecting methyl parathion.
PROCESS BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Yinhui Yi, Wei Zeng, Gangbing Zhu
Summary: A novel nanoprobe based on molybdenum disulfide quantum dots (MoS2 QDs) and cyclodextrin was developed for the fluorescent detection of parathion-methyl, with wide detection scope and excellent selectivity.
Article
Engineering, Chemical
Zhenfu Wang, Jie Gao, Qinghong Shi, Xiaoyan Dong, Yan Sun
Summary: In this study, a facile method for enzyme immobilization was proposed using Cu2+ modified bovine serum albumin (BSA-Cu) induced organic-inorganic hybrid calcium phosphate nanocrystals (CaPs). The BSA-Cu@CaPs nanosheet was used for one-pot purification and immobilization of His-tagged organophosphorus hydrolase (OPH). BSA-Cu@CaPs-OPH exhibited enhanced pH stability, thermal stability, and reusability. This strategy shows promise for the effective biodegradation of organophosphorus pesticides with immobilized enzymes.
CHINESE JOURNAL OF CHEMICAL ENGINEERING
(2023)
Article
Chemistry, Multidisciplinary
Lingfeng Long, Yun Hu, Le Xie, Fubao Sun, Zhenghong Xu, Jinguang Hu
Summary: The study demonstrates a convenient and effective approach for designing a bacterial sensor platform based on bacterial cellulose through CBM2a-assisted cell adhesion strategy, achieving efficient immobilization and sensitive detection of target analyte.
Article
Engineering, Environmental
Zhenfu Wang, Yan Sun
Summary: By in situ encapsulating organophosphorus hydrolase (OPH) into a Zn-doped Co-based ZIF, a hybrid catalyst was developed for the chemoenzymatic cascade conversion of methyl parathion. The hybrid catalyst catalyzed the complete conversion of methyl parathion to 4-aminophenol efficiently, with a maximum cascade conversion rate higher than most reported chemoenzymatic cascade catalysts.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Microbiology
Klaudia Arauzo-Aguilera, Mirva J. Saaranen, Colin Robinson, Lloyd W. Ruddock
Summary: High-value heterologous proteins with disulfide bonds are typically targeted to the periplasm via the Sec pathway, but the Tat system shows potential as an alternative because it can transport fully folded proteins. Previous studies using the TorA signal peptide, a Tat-specific signal peptide, resulted in lower yields and protein degradation. This study demonstrates that the Tat pathway, specifically through the MdoD and AmiC signal peptides, can export the disulfide bond-containing protein YebF to the periplasm and media at high levels.
Article
Biochemistry & Molecular Biology
Nayana Bhat, Bodee Nutho, Chonnikan Hanpaibool, Sarinya Hadsadee, Alisa Vangnai, Thanyada Rungrotmongkol
Summary: This study investigated the binding of different organophosphates (OPs) pesticides to methyl parathion hydrolase (MPH) with cobalt(II) metal ions using computational modeling and molecular dynamics simulations. The findings suggest that the coordination patterns between the pesticides and metal ions differ, indicating a potential impact of metal ions on the functional evolution and divergence within enzyme superfamilies.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2023)
Article
Biotechnology & Applied Microbiology
Sonia Nicchi, Maria Giuliani, Fabiola Giusti, Laura Pancotto, Domenico Maione, Isabel Delany, Cesira L. Galeotti, Cecilia Brettoni
Summary: This study evaluates three approaches for displaying heterologous and homologous lipoproteins on the surface of E. coli, with a focus on the importance of experimental conditions and carrier protein selection in achieving successful protein translocation. The Lpp'OmpA chimera is found to be effective for homologous lipoproteins, while AIDA-I is identified as the best system for mature lipoproteins (especially heterologous ones) without inhibiting growth.
MICROBIAL CELL FACTORIES
(2021)
Review
Food Science & Technology
Hassan Karimi-Maleh, Rozhin Darabi, Mehdi Baghayeri, Fatemeh Karimi, Li Fu, Jalal Rouhi, Dragoi Elena Niculina, Emine Selda Gunduz, E. N. Dragoi
Summary: Methyl parathion (MP) is a commonly used organophosphorus insecticide, and monitoring its residues has attracted attention due to its threat to food safety and the environment. Traditional spectroscopic and chromatographic methods for analyzing MP have limitations such as the need for specialized equipment and extensive sample preparation. Electrochemical sensors, particularly those based on carbon nanomaterials, have emerged as a reliable and quick alternative for detecting MP residues. This study provides an overview of the creation and operation of carbon nanomaterial-based electrochemical sensors for identifying MP residues in various samples, and discusses their potential benefits, drawbacks, and future research prospects.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)
Article
Biotechnology & Applied Microbiology
Xiaoqi Wang, Zhenfu Wang, Linling Yu, Qinghong Shi, Xiaoyan Dong, Yan Sun
Summary: Organophosphorus pesticides (OPPs) degradation is important for environmental bioremediation. This study proposes immobilizing organophosphorus hydrolase (OPH) on mesoporous silica nanoparticles coated with a zwitterionic polymer to enhance enzyme stability and degradation efficiency. The immobilized OPH shows improved stability and activity towards methyl parathion hydrolysis. The coating also promotes substrate enrichment on the carrier, resulting in a lower Michaelis constant and higher catalytic efficiency compared to free OPH.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Yinzhuang Zhu, Yaru Liu, Mingmei Ai, Xiaoqiang Jia
Summary: This study developed a surface display system for carbonic anhydrase using Escherichia coli as the host. The engineered strain showed higher enzyme activity and stability for CO2 capture and mineralization.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Amruta A. Karbelkar, Erin E. Reynolds, Rachel Ahlmark, Ariel L. Furst
Summary: This innovative microbial technology effectively detects and destroys OP pesticides using two engineered strains, showing superior detection capabilities compared to traditional sensors and offering adaptability to various environmental contaminants.
ACS CENTRAL SCIENCE
(2021)
Article
Energy & Fuels
Mei Yuin Joanne Wee, Nur Izyan Wan Azelee, Samson Pachelles, Abdul Munir Abd Murad, Farah Diba Abu Bakar, Rosli Md Illias
Summary: In this study, the feasibility of using cell surface display of xylanase on Escherichia coli for the hydrolysis of hemicellulose from pineapple pomace was investigated. The results showed that the application of this technology is possible and offers great potential for the production of xylooligosaccharides in industry.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Biochemical Research Methods
Wang Ying, Wang YaPing, Huang Can, Ma Lixin, Yan Hong, Min Yong, Liu Xiaoyan, Rao Ben
Summary: Methyl parathion hydrolase (MPH) was efficiently and specifically produced from Plesiomonas sp. M6 using a Pichia pastoris multi-copy expression system. Increasing the number of MPH gene copies enhanced the production of recombinant MPH. The addition of Ni2+, Co2+, and Mg2+ as metal ions significantly increased MPH-R activity. Immobilised MPH-R showed improved stability and activity compared to free MPH-R, demonstrating its potential for industrial applications.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Biochemistry & Molecular Biology
Agnes Ulfig, Roland Freudl
JOURNAL OF BIOLOGICAL CHEMISTRY
(2018)
Review
Biotechnology & Applied Microbiology
Roland Freudl
MICROBIAL CELL FACTORIES
(2018)
Article
Chemistry, Analytical
Daniel Wasik, Ashok Mulchandani, Marylynn V. Yates
Article
Biotechnology & Applied Microbiology
Johannes Hemmerich, Matthias Moch, Sarah Jurischka, Wolfgang Wiechert, Roland Freudl, Marco Oldiges
BIOTECHNOLOGY AND BIOENGINEERING
(2019)
Article
Chemistry, Analytical
Hui Wang, Pankaj Ramnani, Tung Pham, Claudia Chaves Villarreal, Xuejun Yu, Gang Liu, Ashok Mulchandani
Article
Biotechnology & Applied Microbiology
Sarah Jurischka, Astrid Bida, Doris Dohmen-Olma, Britta Kleine, Janko Potzkei, Stephan Binder, Georg Schaumann, Patrick J. Bakkes, Roland Freudl
MICROBIAL CELL FACTORIES
(2020)
Review
Chemistry, Analytical
Mohammed Sedki, Ying Chen, Ashok Mulchandani
Article
Biochemistry & Molecular Biology
Mika Hatada, Noya Loew, Junko Okuda-Shimazaki, Mukund Khanwalker, Wakako Tsugawa, Ashok Mulchandani, Koji Sode
Summary: This study successfully developed a highly sensitive disposable enzyme sensor strip for measuring glycated albumin (GA) using an interdigitated electrode (IDE) as the electrode platform. The IDE enzyme sensor demonstrated superior sensitivity and reproducibility compared to previous sensor designs. Measurement of fructosyl lysine allowed accurate differentiation between healthy and diabetic levels.
Article
Chemistry, Analytical
Hend S. Magar, Rabeay Y. A. Hassan, Ashok Mulchandani
Summary: Electrochemical impedance spectroscopy (EIS) is a powerful technique for analyzing interfacial properties related to bio-recognition events at the electrode surface, with applications in biomedical diagnosis and environmental monitoring. The use of nanomaterials enhances the analytical features of impedimetric biosensors, promoting faster electron transfer and improving sensor reliability and accuracy.
Article
Microbiology
Patrick J. Bakkes, Patrick Lenz, Carolin Mueller, Astrid Bida, Doris Dohmen-Olma, Andreas Knapp, Marco Oldiges, Karl-Erich Jaeger, Roland Freudl
Summary: The development of a new reporter strain with fluorescence protein expression in C. glutamicum has enabled the quantification of protein secretion and optimization of cutinase secretion from Fusarium solani pisi through strategies involving signal peptide library and mutant library. This biosensor-based approach not only significantly increased the secretion efficiency of cutinase, but also improved the specific activity by identifying key mutations in the signal peptide and enzyme structure.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Carolin Mueller, Patrick J. Bakkes, Patrick Lenz, Vera Waffenschmidt, Laura M. Helleckes, Karl-Erich Jaeger, Wolfgang Wiechert, Andreas Knapp, Roland Freudl, Marco Oldiges
Summary: Automated workflows were developed for heterologous production of cutinase in Corynebacterium glutamicum. The best performing signal peptides for cutinase secretion were identified using enzymatic activity and split GFP assay.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Angela Kranz, Tino Polen, Christian Kotulla, Annette Arndt, Graziella Bosco, Michael Bussmann, Ava Chattopadhyay, Annette Cramer, Cedric-Farhad Davoudi, Ursula Degner, Ramon Diesveld, Raphael Freiherr von Boeselager, Kim Gaertner, Cornelia Gaetgens, Tobias Georgi, Christian Geraths, Sabine Haas, Antonia Heyer, Max Huennefeld, Takeru Ishige, Armin Kabus, Nicolai Kallscheuer, Larissa Kever, Simon Klaffl, Britta Kleine, Martina Kocan, Abigail Koch-Koerfges, Kim J. Kraxner, Andreas Krug, Aileen Krueger, Andreas Kueberl, Mohamed Labib, Christian Lange, Christina Mack, Tomoya Maeda, Regina Mahr, Stephan Majda, Andrea Michel, Xenia Morosov, Olga Mueller, Arun M. Nanda, Jens Nickel, Jennifer Pahlke, Eugen Pfeifer, Laura Platzen, Paul Ramp, Doris Rittmann, Steffen Schaffer, Sandra Scheele, Stephanie Spelberg, Julia Schulte, Jens-Eric Schweitzer, Georg Sindelar, Ulrike Sorger-Herrmann, Markus Spelberg, Corinna Stansen, Apilaasha Tharmasothirajan, Jan van Ooyen, Philana van Summeren-Wesenhagen, Michael Vogt, Sabrina Witthoff, Lingfeng Zhu, Bernhard J. Eikmanns, Marco Oldiges, Georg Schaumann, Meike Baumgart, Melanie Brocker, Lothar Eggeling, Roland Freudl, Julia Frunzke, Jan Marienhagen, Volker F. Wendisch, Michael Bott
Summary: Corynebacterium glutamicum is a major host for amino acid production and has been extensively studied in microbial biotechnology. This study presents a large compendium of transcriptional profiles, allowing researchers to investigate gene expression changes under different environmental conditions or genetic modifications. The compendium provides valuable insights into the understanding of C. glutamicum as a microbial cell factory.
Article
Chemistry, Analytical
Guo Zhao, Mohammed Sedki, Shengcun Ma, Claudia Villarreal, Ashok Mulchandani, David Jassby
Article
Genetics & Heredity
Patrick J. Bakkes, Paul Ramp, Astrid Bida, Doris Dohmen-Olma, Michael Bott, Roland Freudl
Article
Chemistry, Analytical
Nuvia M. Saucedo, Yingning Gao, Tung Pham, Ashok Mulchandani