Article
Microbiology
Yaoguang Zhang, Jian Chen, Hao Pan, Xiaojiang Ma, Li Jiang, Qian Zhu, Huanyu Wu, Zhenyu Wang
Summary: In this study, a triplex real-time quantitative PCR assay was developed for the simultaneous differential detection of three protozoan parasites in the human intestinal tract. The assay showed high sensitivity, specificity, and repeatability, and demonstrated good practical application value in fecal samples from patients with diarrhea.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Public, Environmental & Occupational Health
Zaheer Abbas, Muhammad Kasib Khan, Rao Zahid Abbas, Zia Ud Din Sindhu, Muhammad Sohail Sajid, Amir Munir, Abdul Wahid, Arsalan Zafar, Muhammad Adnan Sabir Mughal, Muhammad Imran, Rashid Mehmood, Muhammad Nadeem
Summary: This study evaluated the prevalence of Cryptosporidium and Giardia parasites in different water bodies, soil, and vegetables in Pakistan. The results showed high prevalence of these parasites in sewage water, municipal water, and raw vegetables.
Article
Engineering, Environmental
Asli Onursal, Bulent Icgen
Summary: This study investigated the removal capacities of different types of wastewater treatment plants (WWTPs) for Giardia intestinalis and Cryptosporidium parvum. The results showed that G. intestinalis had lower removal capacities compared to C. parvum in all types of WWTPs tested. Therefore, it is necessary to monitor the discharge of G. intestinalis in WWTPs to reduce potential risks to public health.
WATER ENVIRONMENT RESEARCH
(2023)
Article
Food Science & Technology
Catherine DeMone, J. Trenton McClure, Spencer J. Greenwood, Rebecca Fung, Mei-Hua Hwang, Zeny Feng, Karen Shapiro
Summary: Food and waterborne protozoan pathogens, including Cryptosporidium parvum, Giardia enterica and Toxoplasma gondii, are a global public health concern. A novel 18S amplicon-based next-generation sequencing assay was used to simultaneously detect protozoan pathogens in wild oysters from Prince Edward Island (PEI), with detection in 28% of oysters tested. Protozoan contamination was not associated with harvesting site classifications, indicating fecal indicators may not be sufficient for ensuring food safety.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Naimat Ullah Khan, Tahir Usman, Mian Saeed Sarwar, Hazrat Ali, Ali Gohar, Muhammad Asif, Fazli Rabbani, Rifat Ullah Khan, Nighat Sultana, Nazir Ahmad Khan, Muhammad Mobashar, Assar Ali Shah, Metha Wanapat
Summary: This study investigated the prevalence and risk factors of Cryptosporidium infection in diarrheic sheep in Pakistan, comparing the sensitivity of two diagnostic techniques. The research concluded that molecular detection through PCR is more efficient, specific, and sensitive than simple microscopy for detecting Cryptosporidium infection.
Article
Multidisciplinary Sciences
Naimat Ullah Khan, Tahir Usman, Mian Saeed Sarwar, Hazrat Ali, Ali Gohar, Muhammad Asif, Fazli Rabbani, Rifat Ullah Khan, Nighat Sultana, Nazir Ahmad Khan, Muhammad Mobashar, Assar Ali Shah, Metha Wanapat
Summary: The study investigated the prevalence and risk factors of Cryptosporidium infection in diarrheic sheep in Pakistan using simple microscopy and PCR techniques. Results showed age and sex were important factors affecting prevalence, with molecular detection being more efficient and sensitive compared to simple microscopy.
Article
Biotechnology & Applied Microbiology
Aurelie Bigot-Clivot, Stephanie La Carbona, Catherine Cazeaux, Loic Durand, Elodie Geba, Frank Le Foll, Benoit Xuereb, Houssem Chalghmi, Jitender P. Dubey, Fanny Bastien, Isabelle Bonnard, Melissa Palos Ladeiro, Sandie Escotte-Binet, Dominique Aubert, Isabelle Villena, Alain Geffard
Summary: This study demonstrated that blue mussels can be used as bioindicators for monitoring protozoan parasite contamination in marine waters. The mussels bioaccumulated the parasites over time and showed variation in contamination levels based on water conditions. This highlights the potential of using Mytilus edulis for monitoring parasite contamination in marine environments.
JOURNAL OF APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Angela Sun, Jo-Ann L. Stanton, Peter L. Bergquist, Anwar Sunna
Summary: A universal workflow using filtration and enzyme-based methods has been established to accurately quantify leading pathogens in tap water samples, with the potential to scale up. This method allows for rapid and sensitive quantification with qPCR, bypassing traditional culture-based methods. The workflow shows high quantification efficiencies and offers a rapid, portable, and cost-effective solution for water monitoring.
Article
Multidisciplinary Sciences
Hanieh Mohammad Rahimi, Hamed Mirjalali, Mohammad Reza Zali
Summary: Intestinal parasitic infections are prevalent in many regions, especially in developing countries. This study in Iran found high rates of Blastocystis sp. and Enterocytozoon bieneusi in livestock, with Encephalitozoon spp. mainly detected in chickens. Different animals showed significant correlations with the prevalence and genotypes/subtypes of targeted protozoa.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Amber N. Barnes, Anu Davaasuren, Uyanga Baasandavga, Paul M. Lantos, Battsetseg Gonchigoo, Gregory C. Gray
Summary: The study found that zoonotic enteric parasites Cryptosporidium spp. and Giardia duodenalis are present in human, animal, fly, and water samples in different regions of Mongolia. Risk factors for parasite exposure include drinking water source, access to indoor handwashing site, rural location, and animal ownership. Public health efforts should focus on water, sanitation and hygiene measures, and One Health collaboration between human and animal health sectors.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Article
Public, Environmental & Occupational Health
Amer R. Abdelaziz, Amin Tahoun, Hanem El-Sharkawy, Moustafa M. Abd El-Salam, Mohammed Alorabi, Ahmed M. El-Shehawi, Rasha A. El Meghanawy, Essam E. Toukhy, Ahmed M. Abd El-Salam, Shimaa S. G. Sorour
Summary: This study investigated the prevalence of Cryptosporidium bovis infection in cattle calves from different regions in Egypt. The overall prevalence was 7.27%, with the highest infection rate observed in young suckling calves and during the winter season. Gender and locality did not significantly affect the infection rate. Genetic analysis revealed that the local bovine isolate of C. bovis was highly similar to a human isolate, suggesting a potential zoonotic transmission and a source for human cryptosporidiosis infection in Egypt.
JOURNAL OF TROPICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Gea Guerriero, Kjell Sergeant, Sylvain Legay, Jean-Francois Hausman, Henry-Michel Cauchie, Irshad Ahmad, Khawar Sohail Siddiqui
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2018)
Article
Environmental Sciences
Laetitia Kaas, Leslie Ogorzaly, Gael Lecellier, Veronique Berteaux-Lecellier, Henry-Michel Cauchie, Jeremie Langlet
FOOD AND ENVIRONMENTAL VIROLOGY
(2019)
Article
Ecology
E. Gismondi, H. -M. Cauchie, V. Cruciani, C. Joaquim-Justo
Article
Microbiology
Amine M. Boukerb, Christian Penny, Joelle Serghine, Cecile Walczak, Henry-Michel Cauchie, William G. Miller, Serge Losch, Catherine Ragimbeau, Joel Mossong, Francis Megraud, Philippe Lehours, Lucie Benejat, Michele Gourmelon
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2019)
Review
Microbiology
Maureen Feucherolles, Henry-Michel Cauchie, Christian Penny
Article
Environmental Sciences
Nuria Martinez-Carreras, Leslie Ogorzaly, Cecile Walczak, Christophe Merlin, Emmanuelle Montarges-Pelletier, Christophe Gantzer, Jean-Francois Iffly, Henry-Michel Cauchie, Christophe Hissler
Summary: This study analyzed the concentrations and genogroup distribution of FRNAPHs in the Orne River in France over a two-year period, as well as during four storm runoff events. It found that different driving factors affect FRNAPHs during low flows and storm runoff events, highlighting the importance of measuring at both time scales to better understand phage transport dynamics in surface water.
Article
Thermodynamics
Mariem Jobrane, Achim Kopmeier, Aliza Kahn, Henry-Michel Cauchie, Adel Kharroubi, Christian Penny
Summary: This study presents conceptual improvements on the design and distillation process of a wick type solar still to overcome the low efficiency of the classic system. By combining three ideas, the efficiency of the distiller is significantly improved, resulting in high distillate outputs while maintaining simplicity and low cost. The innovative approach combines mathematical modeling tools and experimental methods to enhance the productivity of the solar still, providing high-quality drinking water.
APPLIED THERMAL ENGINEERING
(2022)
Article
Environmental Sciences
Malte Herold, Aymeric Fouquier d'Herouel, Patrick May, Francesco Delogu, Anke Wienecke-Baldacchino, Jessica Tapp, Cecile Walczak, Paul Wilmes, Henry-Michel Cauchie, Guillaume Fournier, Leslie Ogorzaly
Summary: Monitoring SARS-CoV-2 in wastewater using RT-qPCR to track RNA levels has proven effective for epidemiological surveillance. Identifying specific signature mutations from individual lineages in wastewater samples helps estimate circulating lineages in the population, with allele frequencies of signature mutations corresponding to the occurrence of particular lineages. Additionally, regional clusters can also be discerned based on the genomic sequencing data.
Article
Medicine, General & Internal
Maureen Feucherolles, Morgane Nennig, Soren L. Becker, Delphine Martiny, Serge Losch, Christian Penny, Henry-Michel Cauchie, Catherine Ragimbeau
Summary: The study evaluated the potential of MALDI-TOF MS in pre-screening genetic diversity of C. jejuni, demonstrating high accuracy in predicting CCs, STs, and CTs with protein profiles. The random forest algorithm showed a sensitivity and specificity of up to 97.5% in predicting different STs.
Article
Microbiology
Perrine Florent, Henry-Michel Cauchie, Malte Herold, Stephan Jacquet, Leslie Ogorzaly
Summary: This study examines the influence of abiotic and biotic factors on the distribution of DNA bacteriophages in soil. The results suggest that pH and calcium cations play a significant role in shaping the diversity of bacteriophage populations, and there are important relationships between bacteriophages and bacterial communities.
Review
Environmental Sciences
Perrine Florent, Henry-Michel Cauchie, Leslie Ogorzaly
Summary: This study reviews the potential and application of bacteriophages as hydrological tracers. Bacteriophages have been shown to play an important role in studying hydrological processes such as groundwater, surface water, and wastewater. However, the adsorption process and environmental factors can impact the tracer signal of bacteriophages, highlighting the need for further research and discussion on tracing conditions.
Article
Microbiology
Malte Herold, Louise Hock, Christian Penny, Cecile Walczak, Fatu Djabi, Henry-Michel Cauchie, Catherine Ragimbeau
Summary: As the leading cause of human gastroenteritis, Campylobacter must be monitored extensively using a One Health approach. Wild birds are believed to play a role in spreading recurrent C. jejuni genotypes across countries. To address the challenge of genomic diversity, a filtration method was used to obtain isolates and genotypes were recovered from metagenomic sequencing data. A computational workflow was developed to genotype mixed strains. This study provides a foundation for future research on C. jejuni diversity in environmental and clinical settings.
Article
Genetics & Heredity
Louise Hock, Malte Herold, Cecile Walczak, Alexandra Schoos, Christian Penny, Henry-Michel Cauchie, Catherine Ragimbeau
Summary: This study evaluated the presence of Campylobacter jejuni in wild birds and surface waters, aiming to explore a potential link between these environments and the persistence of recurring lineages infecting humans in Luxembourg. The results showed that a small percentage of C. jejuni found in wild birds overlapped with human strains, suggesting a possible contribution of wild birds to the persistence of these lineages. While wild birds carried C. jejuni, only a few genotypes overlapped with human strains.
MICROBIAL GENOMICS
(2023)
Review
Engineering, Environmental
Mariem Jobrane, Achim Kopmeier, Aliza Kahn, Henry-Michel Cauchie, Adel Kharroubi, Christian Penny
Summary: This article emphasizes the importance and efficiency of using wick type solar stills to produce freshwater, discussing key factors that affect their productivity. Research shows that tilted shape, rotating wick cloth, and parabolic concentrator solar tracking system applications can all significantly improve the performance of distillers.
GROUNDWATER FOR SUSTAINABLE DEVELOPMENT
(2021)
Article
Microbiology
Blandine Fauvel, Henry-Michel Cauchie, Christophe Gantzer, Leslie Ogorzaly
FEMS MICROBIOLOGY ECOLOGY
(2019)