期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 74, 期 7, 页码 2254-2258出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02601-07
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We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of similar to 4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.
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