Article
Biochemistry & Molecular Biology
Marek Kieliszek, Marianna Dourou
Summary: This study evaluated the effects of selenium on the growth and lipid production of two Yarrowia lipolytica strains and found that selenium negatively affected cell mass production and total lipid accumulation, leading to metabolic disorders and impacting cell morphology and lipid droplet distribution. Additionally, selenium influenced fatty acid composition differently in the two strains.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Naghmeh Poorinmohammad, Jing Fu, Bob Wabeke, Eduard J. Kerkhoven
Summary: This study investigated the molecular mechanism underlying lipid accumulation in Yarrowia lipolytica through proteomic analysis. Various cellular functions and biological mechanisms related to lipid accumulation were identified, and genetic engineering of selected targets was used to validate their effects on lipid content, providing new strategies to optimize lipid yield.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Food Science & Technology
Bruno Nicolau Paulino, Adones Sales, Lorena Felipe, Glaucia Maria Pastore, Gustavo Molina, Juliano Lemos Bicas
Summary: Aroma is a crucial attribute of food directly impacting consumer acceptance. In recent decades, interest in biotechnologically produced aroma compounds has grown as a sustainable way to provide natural additives for the food industry. Recent advances in microbial and enzymatic production of aroma compounds demonstrate their high potential for commercial applications.
CURRENT OPINION IN FOOD SCIENCE
(2021)
Article
Multidisciplinary Sciences
Yongshuo Ma, Nian Liu, Per Greisen, Jingbo Li, Kangjian Qiao, Sanwen Huang, Gregory Stephanopoulos
Summary: This study reports the removal of lycopene substrate inhibition in Yarrowia lipolytica by two different strategies, resulting in high carotenoid productivity. The findings provide effective approaches for efficient synthesis of natural products.
NATURE COMMUNICATIONS
(2022)
Article
Biotechnology & Applied Microbiology
Liu-Jing Wei, Xuan Cao, Jing-Jing Liu, Suryang Kwak, Yong-Su Jin, Wei Wang, Qiang Huaa
Summary: This study demonstrated a novel metabolic design for enhancing squalene accumulation in Y. lipolytica by overexpressing mevalonate pathway enzymes, increasing lipid production, and deleting nitrogen catabolite repression-related genes. The results suggest that deletion of URE2 can be utilized to boost hydrophobic target product production in oleaginous yeast strains. This discovery holds potential for future engineering of cell factories to efficiently produce terpenoids.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Agata Fabiszewska, Magdalena Paplinska-Goryca, Paulina Misiukiewicz-Stepien, Malgorzata Woloszynowska, Dorota Nowak, Bartlomiej Zieniuk
Summary: Yarrowia lipolytica yeast is a model species capable of accumulating intracellular lipids. The synthesis of lipids can follow either a de novo pathway or an ex novo pathway. The ex novo pathway is influenced by the limitation of nitrogen source in the medium. It was also found that lipid synthesis in lipid-rich medium mainly occurs via the ex novo pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Jingbo Ma, Yang Gu, Peng Xu
Summary: This study reports a systematic approach to improve the biosynthesis of the cannabinoid precursor olivetolic acid in yeast, leading to a significant increase in the production of the acid. The findings provide a baseline for engineering cannabinoids biosynthesis in oleaginous yeast species.
COMMUNICATIONS BIOLOGY
(2022)
Article
Microbiology
Caleb Walker, Bruce Dien, Richard J. Giannone, Patricia Slininger, Stephanie R. Thompson, Cong T. Trinh
Summary: The study analyzed genetic diversity of undomesticated Y. lipolytica strains, identified genes associated with desirable phenotypes, and compared the differences in xylose utilization and lipid accumulation between these strains and the conventional strain. Proteomic analysis revealed metabolic processes and regulatory elements influencing growth, sugar utilization, and lipid accumulation in undetoxified biomass hydrolysates by natural variants.
Article
Biochemistry & Molecular Biology
Patrycja Szczepanska, Magdalena Rychlicka, Pawel Moroz, Tomasz Janek, Anna Gliszczynska, Zbigniew Lazar
Summary: Phospholipids are a class of lipids with diverse biological functions and are commonly used in lipid replacement therapy. This study found that Yarrowia lipolytica is a promising producer of phospholipids, especially using waste substrates.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Energy & Fuels
Polinati Satya Sagar, Satish Kommoji, Iyyappan Jayaraj, Deepanraj Balakrishnan, Feroz Shaik, Mahendra Vucha, Lalitha Gnanasekaran
Summary: This study investigated the optimization of thermal-diluted acid pretreatment on native grass biomass for simple sugar recovery, followed by lipid bioproduction using Yarrowia lipolytica. The maximum total reducing sugar (34.26 +/- 1.55 g/L) in the native grass hydrolysate was obtained at 163.8 degrees C, 1.57% (v/v) sulfuric acid concentration for 21 min. Detoxified native grass hydrolysate yielded 11.64 +/- 0.84 g/L of lipid with 65.52% (w/w) lipid content after 96 h using Y. lipolytica. These results highlight the efficiency of optimizing thermal-diluted acid pretreatment on native grass biomass for lipid production using Y. lipolytica.
Article
Biochemistry & Molecular Biology
Svetlana Kamzolova, Julia N. Lunina, Vladimir A. Samoilenko, Igor G. Morgunov
Summary: Yarrowia lipolytica yeast is capable of synthesizing citric acid, and the concentration of ammonium sulfate significantly affects its growth and citric acid production.
Article
Biotechnology & Applied Microbiology
Qiang Yan, Tyler B. Jacobson, Zhou Ye, Yoel R. Cortes-Pena, Sarang S. Bhagwat, Susan Hubbard, William T. Cordell, Rebecca E. Oleniczak, Francesca V. Gambacorta, Julio Rivera Vazquez, Eric V. Shusta, Daniel Amador-Noguez, Jeremy S. Guest, Brian F. Pfleger
Summary: Plants produce valuable oleochemical molecules, but meeting the global demand is challenging due to limited land availability and environmental impact. Metabolic engineering offers a solution by transferring oleochemical metabolism to controllable hosts and accessing molecules from non-agricultural plants. This study focuses on rewiring metabolism in Yarrowia lipolytica to synthesize acetyl-diacyl-sn-glycerol (acTAG), which has potential applications as low-grade fuels, lubricants, and emulsifiers. The research establishes acTAG production at g/L scale, explores the impact of lipid bodies, and conducts a techno-economic analysis to determine economic feasibility.
METABOLIC ENGINEERING
(2023)
Article
Biotechnology & Applied Microbiology
Erdem Carsanba, Bilal Agirman, Seraphim Papanikolaou, Patrick Fickers, Huseyin Erten
Summary: The increase in bread wastage leads to ecological problems like methane and CO2 production. To reduce this, a sustainable system of waste bread management should be established. Waste bread has a high potential to be used as feedstock for the production of value-added bio products.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Kanchana R. Kildegaard, Jonathan A. Arnesen, Belen Adiego-Perez, Daniela Rago, Mette Kristensen, Andreas K. Klitgaard, Esben H. Hansen, Jorgen Hansen, Irina Borodina
Summary: This study investigated the potential of using the oleaginous yeast Yarrowia lipolytica to produce specific profiles of gibberellins. By expressing biosynthetic enzymes and upregulating pathways, they successfully increased the production of GA precursors and specific gibberellins, providing a promising resource for recombinant production and identifying bottlenecks in GA biosynthesis.
METABOLIC ENGINEERING
(2021)
Review
Biotechnology & Applied Microbiology
Young-Kyoung Park, Rodrigo Ledesma-Amaro
Summary: Yarrowia lipolytica is a promising candidate for industrial bioproduction of chemicals, fuels, foods, and pharmaceuticals, thanks to its natural and engineered traits. In recent years, extensive research has been conducted to assess its potential, improve its features, and scale up its processes. The increasing number of publications and patents related to Y. lipolytica demonstrates its importance and potential in the field. Despite the remaining challenges, it is expected that Y. lipolytica will become one of the preferred choices for industrial applications in the near future.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Microbiology
Macarena Larroude, Djamila Onesime, Olivier Rue, Jean-Marc Nicaud, Tristan Rossignol
Summary: In this study, the yeast Yarrowia lipolytica was engineered to overproduce pyomelanin by optimizing a chassis strain to increase homogentisic acid synthesis. The overexpression of the 4HPPD gene led to significantly higher pyomelanin yields, with the pathway being strongly induced by phenylalanine supplementation.
Article
Food Science & Technology
Christelle Lopez, Bruno Novales, Hanitra Rabesona, Magalie Weber, Thierry Chardot, Marc Anton
Summary: This study investigated the lipid composition and microstructure of Hemp seed oil bodies (HSOBs) obtained through minimal processing, as well as the effects of homogenization pressure, pH, and minerals on their surface properties and physical stability. HSOBs are rich in balanced PUFA and beneficial components, with their size and stability influenced by pH and minerals.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Food Science & Technology
Sara Mitri, Mohamed Koubaa, Richard G. Maroun, Tristan Rossignol, Jean-Marc Nicaud, Nicolas Louka
Summary: Due to the pleasant rosy scent, there is a huge market demand for the aromatic alcohol 2-phenylethanol (2-PE). The most promising method for its production is through microbial fermentation, particularly using yeasts, which have the ability to produce 2-PE using l-Phe as precursor. Some agro-industrial waste and by-products with high nutritional value can be utilized as suitable media for microbial growth, including the production of 2-PE through yeast fermentation.
Article
Biochemistry & Molecular Biology
Sonia Abreu, Young-Kyoung Park, Camilla Pires de Souza, Lea Vidal, Pierre Chaminade, Jean-Marc Nicaud
Summary: Yarrowia lipolytica is a promising yeast for producing unusual lipids. Recent studies have shown that optimizing substrate combination and metabolic pathways can improve lipid production. In this study, the lipid composition of obese LP and obese L strains was compared, revealing differences in the fatty acid composition and molecular species distributions of various lipids.
Article
Biotechnology & Applied Microbiology
Lea Vidal, Esteban Lebrun, Young-Kyoung Park, Guillaume Mottet, Jean-Marc Nicaud
Summary: In this study, novel bidirectional and hybrid bidirectional promoters were developed, whose expression can be fine-tuned to meet the demand for versatile promoters in the yeast Y. lipolytica. This study provides effective tools for smoothly adjusting the erythritol-inducible co-expression of two target genes in biotechnology applications. BDPs developed in this study have potential applications in the fields of heterologous protein production, metabolic engineering, and synthetic biology.
MICROBIAL CELL FACTORIES
(2023)
Article
Microbiology
Jaroslav Hambalko, Peter Gajdos, Jean-Marc Nicaud, Rodrigo Ledesma-Amaro, Michal Tupec, Iva Pichova, Milan Certik
Summary: Research has found that sex pheromone mixtures of certain insect species can produce unique C16 long conjugated fatty acids with two or three conjugated double bonds. The supplementation of fatty acid methyl esters into the medium resulted in the identification of 20 new fatty acids with two or three double bonds. The study proves that Y. lipolytica is capable of synthesizing C16-conjugated fatty acids, and further optimization could lead to increased production of novel fatty acid derivatives with biotechnologically interesting properties.
Article
Biotechnology & Applied Microbiology
Maria Gorczyca, Jean-Marc Nicaud, Ewelina Celinska
Summary: Resistance to environmental stress and synthesis of recombinant proteins (r-Prots) are complex biological traits that rely on multiple genes. Modifying the operation of transcription factors (TFs) associated with these traits can be a strategy to engineer them. This study examined the implications of five selected TFs in stress resistance and r-Prot synthesis in Yarrowia lipolytica. Over-expression or deletion of the TFs in a host strain resulted in significant changes in growth and r-Prot yields under specific conditions. Mathematical modeling assisted in understanding the contribution of individual TFs and identifying new functions of the studied TFs.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Veronika Urbanikova, Young-Kyoung Park, Daniela Krajciova, Mehdi Tachekort, Milan Certik, Ioana Grigoras, Roman Holic, Jean-Marc Nicaud, Peter Gajdos
Summary: In this study, the oleaginous yeast Yarrowia lipolytica was used as a host for the production of Punicic acid (PuA). By supplementing the medium with pomegranate seed oil, lipids containing PuA esters were accumulated up to 31.2% in Y. lipolytica. Strategies involving the expression of the bifunctional fatty acid conjugase/desaturase gene from Punica granatum (PgFADX) allowed for de novo production of PuA in Y. lipolytica. The optimized promoter for PgFADX expression resulted in a PuA production of 36.6 mg/L. These findings highlight the potential of Y. lipolytica as a promising host for PuA production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Georgio Nemer, Nicolas Louka, Paul Rabiller Blandin, Richard G. Maroun, Eugene Vorobiev, Tristan Rossignol, Jean-Marc Nicaud, Erwann Guenin, Mohamed Koubaa
Summary: This study focuses on the biosynthesis of a mixture of violacein and deoxyviolacein using a genetically modified Y. lipolytica strain as a production chassis. The intracellular pigments were extracted and purified using column chromatography. The optimal separation between the pigments was achieved using an ethyl acetate/cyclohexane mixture with different ratios, followed by analysis using thin-layer chromatography and nuclear magnetic resonance.
Article
Biotechnology & Applied Microbiology
Lea Vidal, Zehui Dong, Kim Olofsson, Eva Nordberg Karlsson, Jean-Marc Nicaud
Summary: Yarrowia lipolytica is a suitable yeast for producing foreign proteins. A set of 18 chromogenic cloning vectors were developed, each containing an excisable auxotrophic selective marker and a different promoter. An improved recipient strain was developed by eliminating filamentation and introducing an auxotrophy for lysine. The optimal targeting sequence for Rhizopus oryzae ROL lipase secretion was determined and higher lipase activity was achieved with certain promoters.
FEMS YEAST RESEARCH
(2023)
Article
Biochemical Research Methods
Esteban Lebrun, Vasily Shenshin, Cecile Plaire, Vincent Vigneres, Theo Pizette, Bruno Dumas, Jean-Marc Nicaud, Guillaume Mottet
Summary: The search for new antibodies is time-consuming and expensive due to repeated library screenings. A new antibody screening platform based on the encapsulation of antibody-secreting yeast cells into picoreactor droplets is proposed in this study. This platform enables high-throughput screening of millions of antibodies per day and is useful in various contexts.
Review
Agronomy
Thierry Chardot
Summary: This article introduces some interesting lipids and their possible uses, as well as resources and methods for large-scale production of these lipids in different hosts.
OCL-OILSEEDS AND FATS CROPS AND LIPIDS
(2022)
Article
Biotechnology & Applied Microbiology
Wendy Al Sahyouni, Sally El Kantar, Anissa Khelfa, Young-Kyoung Park, Jean-Marc Nicaud, Nicolas Louka, Mohamed Koubaa
Summary: This study investigated the production of odd-chain fatty acid-enriched lipids through fermentation using a genetically engineered strain. The optimal conditions for maximizing cell density and fatty acid content were determined, and the results show potential for industrial production.
FERMENTATION-BASEL
(2022)
Review
Biotechnology & Applied Microbiology
Young-Kyoung Park, Cristina Gonzalez-Fernandez, Raul Robles-Iglesias, Lea Vidal, Pierre Fontanille, Christian Kennes, Elia Tomas Pejo, Jean-Marc Nicaud, Patrick Fickers
Summary: This review discusses the processes of creating valuable molecules from organic wastes-derived VFAs, the pathways used by oleaginous yeast Yarrowia lipolytica to metabolize such molecules, and the relationship between organic waste composition, anaerobic digestion, and VFA profiles. It also summarizes the current knowledge about VFA toxicity, the metabolic pathways of VFAs, and the metabolic engineering strategies in Y. lipolytica for producing value-added biobased compounds from VFAs.
FEMS YEAST RESEARCH
(2021)
Review
Biotechnology & Applied Microbiology
Georgio Nemer, Nicolas Louka, Eugene Vorobiev, Dominique Salameh, Jean-Marc Nicaud, Richard G. Maroun, Mohamed Koubaa
Summary: The production of pigments using single cell microorganisms is emerging as a sustainable alternative to conventional synthesis methods heavily reliant on petrochemicals. Research is focused on producing pigments coveted for their unique qualities beyond just dyeing abilities. Efforts are also directed towards developing effective cell disruption methods to ensure successful recovery of biosynthetic pigments.
FERMENTATION-BASEL
(2021)
