Article
Food Science & Technology
Wenxiu Zhu, Jingzhu Gao, Hongli Liu, Jiaxiu Liu, Tong Jin, Ningbo Qin, Xiaomeng Ren, Xiaodong Xia
Summary: Sodium butyrate exhibits antibiofilm effects against V. parahaemolyticus by inhibiting biofilm formation, disrupting cellular metabolism, reducing cell viability within the biofilm, decreasing bacterial motility and surface hydrophobicity, and interfering with quorum sensing. Additionally, sodium butyrate down-regulates genes associated with biofilm formation in V. parahaemolyticus, suggesting its potential as a potent agent in combating biofilm in food industries.
Article
Biochemistry & Molecular Biology
Jiawen Liu, Jinrong Tong, Qian Wu, Jing Liu, Mengqi Yuan, Cuifang Tian, Huan Xu, Pradeep K. Malakar, Yingjie Pan, Yong Zhao, Zhaohuan Zhang
Summary: In this study, a high-throughput virtual screening was conducted to identify compounds that can inhibit LolB and exhibit antibacterial activity against Vibrio parahaemolyticus. Procyanidin, stevioside, troxerutin, and rutin were found to have both high binding affinity with LolB and concentration-dependent antibacterial activity. These compounds could serve as potential antibacterial agents against V. parahaemolyticus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Yuanyuan Meng, Lili Mu, Yinhui Li, Man Yu, Haiquan Liu, Yingjie Pan, Yong Zhao
Summary: This study investigated the expression patterns of two-component system (TCS) in Vibrio parahaemolyticus with different genotypes and explored the differences in TCS between strains. The results showed similarities in the TCS expression pattern between certain genotypes, while another genotype had the least similar pattern. Analysis of biological information revealed that different TCS regulations may influence strains' growth, antibiotic resistance, biofilm, and virulence. The different TCS regulatory abilities of strains might be one of the reasons for diverse biological characteristics and different environmental adaptations.
Article
Food Science & Technology
Hongli Liu, Wenxiu Zhu, Yu Cao, Jingzhu Gao, Tong Jin, Ningbo Qin, Xiaodong Xia
Summary: Punicalagin showed significant anti-biofilm activity at sub-inhibitory concentrations, decreasing bacterial motility and metabolic activity in biofilms. It also inhibited EPS production, reduced exopolysaccharides in biofilms, and decreased Raman peak intensities of biofilm components. Punicalagin significantly inhibited V. parahaemolyticus adhesion to Caco-2 cells and downregulated genes related to biofilm formation and virulence.
Article
Microbiology
Miaomiao Zhang, Liyan Cai, Xi Luo, Xue Li, Tingting Zhang, Fei Wu, Yiquan Zhang, Renfei Lu
Summary: In this study, the effects of sublethal dose of chloramphenicol on Vibrio parahaemolyticus were investigated using various phenotypic assays and RNA sequencing. The results showed that the growth rate, biofilm formation, c-di-GMP synthesis, motility, cytoxicity, and adherence activity of V. parahaemolyticus were significantly decreased by the sublethal dose of chloramphenicol. RNA-seq analysis revealed differential expression of genes related to antibiotic resistance, virulence, biofilm formation, and regulation. The findings suggest that chloramphenicol has a significant impact on the physiology of V. parahaemolyticus.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Food Science & Technology
Wenxiu Zhu, Jiaxiu Liu, Yue Zou, Shugang Li, Dongyun Zhao, Haisong Wang, Xiaodong Xia
Summary: This study found that laurel essential oil (LEO) can effectively reduce biofilm formation of Vibrio parahaemolyticus, and it affects its metabolic activity, biofilm structure, and attachment ability. Therefore, it has potential applications in the food industry.
Article
Fisheries
Qianni Fu, Jinxia Liu, Tianjiao Ren, Zining Zhang, Zihang Ma, Zhenyu Lan, Yitao Duan, Ziwei Liang, Boyu Chen, Yan Zhang, Peng Zhu, Yongyan Liao
Summary: Heat shock proteins (HSPs) help maintain protein homeostasis and protect organisms from stress. In this study, the HSP10 and HSP40 genes of Eriocheir hepuensis were cloned and their expression patterns were analyzed. The results suggest that these proteins may play a role in protecting E. hepuensis from bacterial infections.
Article
Engineering, Environmental
Peyman Namadi, Zhiqiang Deng
Summary: A study developed forecasting models with different lead times using the Random Forest method to predict the level of V.p in oysters, based on data from two geographic locations. Results showed that antecedent environmental conditions significantly influence V.p abundance, and the models can effectively predict V.p abundance 1-4 days in advance, with model performance decreasing as lead time increases.
Article
Immunology
Xin Tan, Jun Qiao, Hedan Li, Danyang Huang, Xiaoqing Hu, Xiaoyuan Wang
Summary: This study investigated the metabolic regulation profile of Vibrio parahaemolyticus under polymyxin B stimulation, revealing significant changes in gene transcription and activation of cellular antioxidant systems to prevent bacteria from oxidant stress. The findings suggest new insights into polymyxin B-related stress response in V. parahaemolyticus, which could be beneficial for developing novel drugs for infection.
MICROBIAL PATHOGENESIS
(2021)
Article
Food Science & Technology
Corinne Audemard, Tal Ben-Horin, Howard I. Kator, Kimberly S. Reece
Summary: This study found that concentrations of bacteria in seawater can predictably reflect concentrations of the same bacteria in oysters. In regions with low tidal range, seawater samples can be used as an alternative method to assess the risk associated with oysters.
Article
Microbiology
Iker Fernandez-Velez, Gorka Bidegain, Tal Ben-Horin
Summary: Temperature is a critical factor for the growth of Vibrio parahaemolyticus in oysters, with higher temperatures favoring rapid growth and increasing the risk of gastroenteritis in humans. A continuous time model was developed to predict the pathogen growth in oysters under varying ambient temperature. The model showed promise in understanding the V. parahaemolyticus-oyster system and assessing the public health impact of raw oyster consumption.
Article
Fisheries
Petcharat Namwong, Shuang Wang, Qing Kong, Haijin Mou, Lei Ma, Prapansak Srisapoome
Summary: This study investigated the differential expression and biological processes of immune-related genes, primarily pattern recognition receptors (PRRs), in the digestive gland and mantle tissues of Crassostrea gigas in response to V. parahaemolyticus infection. The results showed that the two tissues exhibited different immune mechanisms and gene expressions, with the digestive gland tissues demonstrating immune mechanisms against bacterial adhesion and phagocytosis, and the mantle tissue showing oxidative stress and peroxidase reactions. Several PRRs-related genes were up-regulated in response to infection, indicating their critical role in the defense mechanism. The study provides valuable information on the immune response of C. gigas to V. parahaemolyticus infection and identifies potential candidate genes for further studies.
Article
Biotechnology & Applied Microbiology
Kyle D. Brumfield, Arlene J. Chen, Mayank Gangwar, Moiz Usmani, Nur A. Hasan, Antarpreet S. Jutla, Anwar Huq, Rita R. Colwell
Summary: The incidence of vibriosis is increasing globally due to changing climatic conditions that promote the growth of pathogenic Vibrio species. Environmental factors, such as temperature, salinity, total chlorophyll a, dissolved oxygen, and pH, were found to impact the occurrence of pathogenic Vibrio species. Long-term monitoring revealed an increase in Vibrio populations in the Chesapeake Bay region. The findings highlight the importance of predicting and managing Vibrio-related diseases in the face of climate change.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Food Science & Technology
Xiaojun Zhong, Zihao Pan, Yanjuan Mu, Yinchu Zhu, Yue Zhang, Jiale Ma, Menghua Yang, Huochun Yao
Summary: In this study, 152 V. parahaemolyticus strains were isolated from shellfish, shrimp, crab, and snails in East China, and the genetic diversity, population structure, and virulence profiles of these strains were analyzed. The findings revealed high genetic diversity among the strains and highlighted the potential risk of infectious outbreaks.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Fisheries
Luyue Li, Jiaqi Lu, Pingping Zhan, Qiongfen Qiu, Jiong Chen, Jinbo Xiong
Summary: This study investigated how elevated temperature and nutrient stimulate the expression profiles of Vibrio parahaemolyticus, a pathogen causing shrimp acute hepatopancreatic necrosis disease (AHPND). The results showed that elevated temperature and nutrient significantly altered the transcriptional patterns of V. parahaemolyticus, leading to differential expression of various genes. The findings provide comprehensive insights into the molecular mechanism of V. parahaemolyticus pathogenicity and partially explain the increased disease frequency under conditions of increasing temperature and eutrophication.