Article
Biotechnology & Applied Microbiology
Toru Jojima, Takafumi Igari, Ryoji Noburyu, Akira Watanabe, Masako Suda, Masayuki Inui
Summary: Introducing the ED pathway in metabolically engineered Corynebacterium glutamicum enhances glucose consumption and ethanol production. The presence of the ED pathway does not alter the intracellular NADH/NAD(+) ratio but slightly increases the ATP/ADP ratio.
BIOTECHNOLOGY FOR BIOFUELS
(2021)
Article
Plant Sciences
Gong Xiaobao, Xiong Li, Bi Caihong, Zhang Baoshun
Summary: Dios treatment significantly improved glucose metabolism in KK-Ay diabetic mice by regulating C. glu via the IRS/PI3K/AKT signaling pathway and reshaped the unbalanced gut microbiota. Our study provided evidence for the potential application of Dios in the treatment of T2DM.
Review
Biotechnology & Applied Microbiology
Yota Tsuge, Akira Yamaguchi
Summary: Corynebacterium glutamicum is widely used in industrial production of amino acids, organic acids, and other value-added compounds. It demonstrates good adaptability to oxygen levels and is capable of growth and metabolism under both anaerobic and aerobic conditions.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Koichi Toyoda, Masayuki Inui
Summary: When transitioning from exponential to stationary growth phases, bacterial metabolism shifts from aerobic respiration to fermentation in response to limited oxygen availability. The ldhA gene in Corynebacterium glutamicum encodes l-lactate dehydrogenase, responsible for l-lactate production, and is controlled by transcription regulators SugR, LldR, and GlxR. GlxR has been shown to act as a transcriptional activator of ldhA, affecting both its promoter activity and l-lactate production.
Article
Microbiology
Tobias Busche, Hana Dostalova, Lenka Rucka, Jiri Holatko, Ivan Barvik, Vaclav Stepanek, Miroslav Patek, Joern Kalinowski
Summary: The sigma H and sigma E subunits of Corynebacterium glutamicum RNA polymerase belong to Group 4 of sigma factors, also called extracytoplasmic function (ECF) sigma factors. While the genes of the C. glutamicum sigma(Eta) regulon involved in heat and oxidative stress response have been studied, the genes of the sigma(E) regulon, involved in cell surface stress response, have not been explored until now. Differential gene expression analysis revealed a significant overlap between the sigma(Eta) and sigma(E) regulons, and identified genes involved in protein quality control, regulation of Clp proteases, and membrane integrity maintenance.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Endocrinology & Metabolism
Chunjun Zhan, Namil Lee, Guangxu Lan, Qingyun Dan, Aidan Cowan, Zilong Wang, Edward E. K. Baidoo, Ramu Kakumanu, Bridget Luckie, Rita C. Kuo, Joshua McCauley, Yuzhong Liu, Luis Valencia, Robert W. Haushalter, Jay D. Keasling
Summary: Corynebacterium glutamicum is a promising host for producing valuable polyketides, but propionate addition inhibits its growth. This study identifies the accumulation of propionyl-CoA and methylmalonyl-CoA as the cause of growth inhibition and demonstrates that it can be relieved by introducing methylmalonyl-CoA-dependent polyketide synthases. Laboratory evolution is used to increase polyketide production by targeting mutations in germicidin synthase and citrate synthase. These findings highlight the importance and potential of C. glutamicum in polyketide production.
Article
Plant Sciences
Shaoyan Zheng, Jingqin Lu, Di Yu, Jing Li, Hai Zhou, Dagang Jiang, Zhenlan Liu, Chuxiong Zhuang
Summary: This study revealed that OsHXK1 is a positive regulator of rice leaf senescence by mediating glucose accumulation and inducing an increase in ROS levels. OsHXK1 upregulation leads to premature senescence of rice leaves, decreased chlorophyll levels, and damage to chloroplast structure, along with increased glucose and ROS levels, and programmed cell death signals at an early stage.
Article
Biotechnology & Applied Microbiology
Feng Peng, Jing Chen, Xiuxia Liu, Ye Li, Chunli Liu, Yankun Yang, Zhonghu Bai
Summary: The PhoPR system in corynebacteria and mycobacteria responds to oxygen deficiency, regulating pathways involved in sustaining energy levels under low oxygen conditions. PhoP is significantly upregulated upon decrease of dissolved oxygen and impacts the expression of genes related to global regulation and energy metabolism.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Hedan Li, Daqing Xu, Yuantao Liu, Xin Tan, Jun Qiao, Zihan Li, Bin Qi, Xiaoqing Hu, Xiaoyuan Wang
Summary: In this study, a gene encoding ketoacyl reductase in the biosynthetic pathway of mycolate was deleted in C. glutamicum, resulting in increased production of L-glutamate. Transcriptomic analysis revealed the up-regulation of certain genes and down-regulation of others possibly contributing to the overproduction of L-glutamate in C. glutamicum Delta cmrA.
BIOCHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Huanmin Du, Yuting Qi, Jinfang Qiao, Lingcong Li, Liang Wei, Ning Xu, Li Shao, Jun Liu
Summary: This study revealed the regulatory mechanism of OxyR on intracellular sulfane sulfur removal in C. glutamicum. OxyR directly regulated sulfane sulfur levels by controlling the expression of katA and nrdH, and also affected the sulfur assimilation and L-cysteine synthesis pathways. These findings provide insights into sulfur regulatory mechanisms and have potential applications in constructing robust cell factories for sulfur-containing amino acids.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Article
Microbiology
Hedan Li, Daqing Xu, Xin Tan, Danyang Huang, Yu Huang, Guihong Zhao, Xiaoqing Hu, Xiaoyuan Wang
Summary: In this study, a mutant strain ASYA of Corynebacterium glutamicum was constructed to be unable to synthesize trehalose. ASYA grew as well as the control strain ATCC13869 in fermentation medium, but produced significantly more L-glutamate. In minimal medium, ASYA grew worse but excreted more L-glutamate. The major lipid in ASYA was phospholipids, while in ATCC13869 it was glucose monocorynomycolate, trehalose dicorynomycolate, and phospholipids.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Jiaoqi Gao, Wei Yu, Yunxia Li, Mingjie Jin, Lun Yao, Yongjin J. Zhou
Summary: Engineered yeast Ogataea polymorpha has been used to co-utilize glucose and xylose for the production of free fatty acids (FFAs) and 3-hydroxypropionic acid (3-HP) from lignocellulose.
NATURE CHEMICAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Tantan Gao, Yan Li, Yunrong Chai, Qi Wang, Mingzheng Ding
Summary: Our study demonstrates that the colonization ability of Bacillus cereus 905 on wheat roots is influenced by the sodA2 gene and the sigB gene, playing important roles in response to environmental stress conditions. The sigB mutant showed reduced survival and defective biofilm formation when exposed to ethanol, acid, heat, oxidative stress, or glucose starvation.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Ying Liu, Tao Li, Chunxia Zhang, Wenli Zhang, Nan Deng, Lynnette M. A. Dirk, A. Bruce Downie, Tianyong Zhao
Summary: Overexpression of the ZmRAFS gene in maize increases raffinose content in leaves, reduces water loss, and enhances plant drought stress tolerance.
Article
Gastroenterology & Hepatology
Maria J. Gonzalez-Rellan, Eva Novoa, Natalia da Silva Lima, Amaia Rodriguez, Christelle Veyrat-Durebex, Samuel Seoane, Begona Porteiro, Marcos F. Fondevila, Uxia Fernandez, Marta Varela-Rey, Ana Senra, Cristina Iglesias, Adriana Escudero, Miguel Fidalgo, Diana Guallar, Roman Perez-Fernandez, Vincent Prevot, Markus Schwaninger, Miguel Lopez, Carlos Dieguez, Roberto Coppari, Gema Fruhbeck, Ruben Nogueiras
Summary: The transcription factor p63 plays a key role in glucose metabolism by regulating SIRT1 and affecting blood glucose levels, thus influencing glucose homeostasis.
Article
Biotechnology & Applied Microbiology
Yota Tsuge, Naoto Kato, Shogo Yamamoto, Masako Suda, Masayuki Inui
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Tetsu Shimizu, Haruhiko Teramoto, Masayuki Inui
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2019)
Article
Microbiology
Akiyoshi Higo, Eri Nishiyama, Kota Nakamura, Yukako Hihara, Shigeki Ehira
JOURNAL OF BACTERIOLOGY
(2019)
Editorial Material
Biochemical Research Methods
Sung Ok Han, Masayuki Inui, Yong-Su Jin
BIOTECHNOLOGY JOURNAL
(2019)
Article
Biotechnology & Applied Microbiology
Shinichi Oide, Yuya Tanaka, Akira Watanabe, Masayuki Inui
ENZYME AND MICROBIAL TECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Tetsu Shimizu, Haruhiko Teramoto, Masayuki Inui
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Satoshi Hasegawa, Toru Jojima, Masako Suda, Masayuki Inui
METABOLIC ENGINEERING
(2020)
Article
Biotechnology & Applied Microbiology
Hiroto Uchikura, Koichi Toyoda, Hiroki Matsuzawa, Hikaru Mizuno, Kazuaki Ninomiya, Kenji Takahashi, Masayuki Inui, Yota Tsuge
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Takahisa Kogure, Masako Suda, Kazumi Hiraga, Masayuki Inui
Summary: Protocatechuic acid (PCA) is a natural bioactive phenolic acid that can be produced by Corynebacterium glutamicum through the DHS and 4-HBA pathways. Simultaneous utilization of both pathways is an efficient method for PCA production, with the highest reported productivity reaching 82.7 g/L.
METABOLIC ENGINEERING
(2021)
Article
Biochemical Research Methods
Shiori Nakazawa, Osamu Imaichi, Takahisa Kogure, Takeshi Kubota, Koichi Toyoda, Masako Suda, Masayuki Inui, Kiyoto Ito, Tomokazu Shirai, Michihiro Araki
Summary: The technique involves computational suggestion of genetic modifications based on design history, retrieval of well-known designs related to the history, and enhancement of production through experimental modification. The successful utilization of former cycles suggests relevant designs worth testing, ultimately aiding in the development of bioprocessing.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Chemistry, Physical
Haruhiko Teramoto, Tetsu Shimizu, Masako Suda, Masayuki Inui
Summary: This study explored the genetic engineering of Escherichia coli for hydrogen production. The introduction of exogenous hydrogenase and inactivation of endogenous hydrogenases resulted in both hydrogen production and uptake. Disabling the pathways for ethanol, lactate, and succinate production significantly increased hydrogen production. Unexpectedly, high expression of exogenous hydrogenase completely repressed hydrogen production, and the introduction of heterologous enzyme repressed the endogenous hydrogen production ability.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Microbiology
Koichi Toyoda, Riki Sugaya, Akihiro Domon, Masako Suda, Kazumi Hiraga, Masayuki Inui
Summary: Understanding the metabolic pathways and regulation of amino acids is crucial for optimizing amino acid production. This study identified and characterized the ans operon that is involved in the catabolism of L-Asn in Corynebacterium glutamicum R. The operon consists of genes encoding a transcriptional regulator (AnsR), asparaginase (AnsA2), aspartase (AspA2), and permease (AnsP). The enzymes and permease encoded by this operon are essential for the utilization of L-Asn. The study also revealed the regulatory mechanism of the operon, with AnsR acting as a transcriptional repressor that is inhibited by L-Asn.
Article
Biotechnology & Applied Microbiology
Tetsu Shimizu, Haruhiko Teramoto, Masayuki Inui
Summary: This study provides a novel approach for increasing the yield of photofermentative H-2 production from acetate by purple nonsulfur phototrophic bacteria. This study further suggests that polyhydroxyalkanoate is not only a storage substance for carbon and energy in bacteria, but may also act as a signaling molecule that mediates bacterial metabolic adaptations to specific environments.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Chemistry, Physical
Haruhiko Teramoto, Masako Suda, Masayuki Inui
Summary: This study investigates the inhibitory compounds in dilute sulfuric acid-pretreated corn stover on hydrogen production by Escherichia coli. The results indicate that acetate is the primary inhibitory factor.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Article
Biotechnology & Applied Microbiology
Akiyoshi Higo, Shigeki Ehira
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2019)