期刊
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
卷 74, 期 11, 页码 3419-3425出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00476-08
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资金
- NIGMS NIH HHS [GM047446, R01 GM047446, GM069508, R01 GM047446-16, R01 GM069508-02, R01 GM069508] Funding Source: Medline
A Tn7 donor plasmid, pTn7SX, was constructed for use with the model gram-positive bacterium Bacillus subtilis. This new mini-Tn7, mTn7SX, contains a spectinomycin resistance cassette and an outward-facing, xylose-inducible promoter, thereby allowing for the regulated expression of genes downstream of the transposon. We demonstrate that mTn7SX inserts are obtained at a high frequency and occur randomly throughout the B. subtilis genome. The utility of this system was demonstrated by the selection of mutants with increased resistance to the antibiotic fosfomycin or duramycin.
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