Article
Food Science & Technology
Marlen Petersen, Luyao Ma, Xiaonan Lu
Summary: In this study, a method combining LAMP and PMA treatment was developed for detecting VBNC C. jejuni in agri-foods. The method demonstrated high specificity and quantitative capacity, allowing for rapid and sensitive determination of the pathogenic bacteria in contaminated agricultural products, reducing the risk to public health.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Sabin Poudel, Linan Jia, Mark A. Arick Ii, Chuan-Yu Hsu, Adam Thrash, Anuraj T. Sukumaran, Pratima Adhikari, Aaron S. Kiess, Li Zhang
Summary: Campylobacter jejuni is a common food-borne pathogen causing gastroenteritis in the United States. This study aimed to identify potential vaccine candidates against C. jejuni through genomic analysis and gene expression analysis during host-pathogen interactions. Three candidate antigens, including phospholipase A, TonB dependent vitamin B12 transporter, and cytolethal distending toxin subunit B, were predicted and found to be upregulated in different C. jejuni strains.
Article
Biotechnology & Applied Microbiology
Benjamin Duque, Sandrine Reze, Albert Rossero, Jeanne-Marie Membre, Sandrine Guillou, Nabila Haddad
Summary: Broiler meat is considered a significant source of Campylobacter jejuni, a foodborne pathogen. Stress conditions during slaughtering can affect bacterial adaptation and resistance, leading to changes in gene expression. RT-qPCR was used to measure gene expression in 44 C. jejuni genes after thermal stresses, revealing differential expression in 26 genes. The study provides insights into the molecular mechanisms of bacterial stress response in real-life processing conditions.
Article
Agriculture, Dairy & Animal Science
Paul Dawson, Ahmet Buyukyavuz, Claudia Ionita, Julie Northcutt
Summary: The combination of PCR and DNA extraction techniques can be used to enumerate Campylobacter spp. from poultry gastrointestinal tract samples. The study developed a DNA standard curve for Campylobacter spp. DNA primers and a cell/genomic DNA extraction protocol for complex samples. The results showed that qPCR and standard plate count methods were highly correlated for enumerating Campylobacter spp. in the specified range.
Article
Food Science & Technology
Franca Rossi, Ilaria Del Matto, Paola Manocchio, Maria Antonietta Saletti, Luciano Ricchiuti, Lucio Marino
Summary: The aim of this study was to investigate the survival of Campylobacter jejuni in dairy matrices by viability qPCR. The cheese outbreak strain, C. jejuni 11 218, showed enhanced tendency to become viable but nonculturable (VBNC) in UHT milk, as well as in raw and fermented milk and cheese. Viability qPCR also detected viable Campylobacter spp. in 40.9% of 25 mL raw milk samples that were negative on culture. The results highlight the value of viability qPCR in estimating Campylobacter risk in dairy products.
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
(2023)
Article
Microbiology
Anand B. Karki, Bhuwan Khatri, Mohamed K. Fakhr
Summary: RNA-seq was used to analyze the differentially expressed genes (DEGs) in C. coli HC2-48 and C. jejuni OD2-67 transcriptomes under different temperature conditions. In C. coli HC2-48, genes involved in flagellar assembly were significantly impacted by low temperatures (4°C), while genes related to the ribosome and ribonucleoprotein complex were modulated in C. jejuni OD2-67 at 4°C. Although more DEGs were observed in the chicken juice medium compared to Mueller-Hinton broth, there were no common genes expressed at all incubation times, indicating that the food matrix environment is not the sole determinant of differential expression in Campylobacter spp. at low temperatures.
Article
Food Science & Technology
Ana Rita Barata, Barbara Nunes, Ricardo Oliveira, Hugo Guedes, Carina Almeida, Maria Jose Saavedra, Gabriela Jorge da Silva, Goncalo Almeida
Summary: The growing belief in the health benefits of natural products, such as raw milk, has led to behaviors that can contribute to food safety issues and increased healthcare costs. This study investigated the occurrence and seasonality of Campylobacter spp. in raw cow's milk and the potential routes of contamination in dairy farm environments. The findings highlight the potential risk of campylobacteriosis associated with consuming raw milk and emphasize the importance of routine surveillance in dairy farms for zoonotic pathogens.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Microbiology
Andrea Laconi, Ilenia Drigo, Nicola Palmieri, Lisa Carraro, Elena Tonon, Raffaela Franch, Luca Bano, Alessandra Piccirillo
Summary: The study revealed that Campylobacter isolates from chicken livers possessed a higher number of virulence-associated genes, which might contribute to the development of pathological lesions in these isolates within the host.
VETERINARY MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Kay M. Russell, Jacqueline Smith, Abi Bremner, Cosmin Chintoan-Uta, Lonneke Vervelde, Androniki Psifidi, Mark P. Stevens
Summary: The transcriptional response in the caecal tonsils of inbred chickens to Campylobacter colonisation is limited, but there are a large number of differentially transcribed genes between chicken lines 6(1) and N which may explain the variation in heritable resistance to C. jejuni.
Article
Microbiology
Saeed Sharifi, Bita Bakhshi, Shahin Najar-Peerayeh
Summary: The study demonstrated the significant contribution of the CmeABC efflux pump in conferring high-level resistance to tetracycline and ciprofloxacin, along with the involvement of the tetO gene and point mutation of the gyrA gene. Genotyping revealed no clonal correlation among resistant strains, suggesting distinct evolution of tetracycline and ciprofloxacin resistant genotypes among the isolates.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2021)
Article
Microbiology
Teruyo Ojima-Kato, Satomi Nagai, Akane Fujita, Junko Sakata, Hiroto Tamura
Summary: Identification of microorganisms by MALDI-TOF MS has become popular in the past 20 years. The Strain Solution ver. 2 software with MALDI-TOF MS allows accurate discrimination of serotypes and strains beyond the genus and species level by creating a theoretical mass-based database. This study constructed a theoretical mass database with validated biomarkers for proteotyping Campylobacter jejuni and achieved promising results.
Article
Biochemistry & Molecular Biology
Su-jin Lee, Si Yeon Ahn, Han Byeol Oh, Seung Yeon Kim, Wan Seok Song, Sung-il Yoon
Summary: The recombination mediator complex RecFOR is necessary for initiating homologous recombination in bacteria. Our study focused on Campylobacter jejuni, a bacteria that lacks the recF gene and uses only RecR and RecO for recombination initiation. We determined the crystal structure of cjRecR and found differences in its binding properties compared to RecR from other bacteria that express RecF.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Pattarapong Wenbap, Yortyot Seetang-Nun, Taradon Luangtongkum, Pongsak Khunrae, Pravate Tuitemwong, Triwit Rattanarojpong
Summary: Campylobacteriosis is caused by infection of Campylobacter spp. in humans, mainly by C. jejuni. This study successfully designed a chimeric recombinant antigen named multiepitope antigen (MEA) using in silico techniques, which showed high antigenicity and specificity in detecting both C. jejuni and C. coli. The produced anti-MEA antibody had a high titer and did not cross-react with non-Campylobacter spp.
PROTEIN EXPRESSION AND PURIFICATION
(2021)
Article
Multidisciplinary Sciences
Medelin Ocejo, Beatriz Oporto, Jose Luis Lavin, Ana Hurtado
Summary: A long-term surveillance study on dairy cattle farms revealed that C. jejuni was more prevalent than C. coli, and resistance rates were higher in calves. Some genotypes persisted throughout the study while others were sporadically detected. Acquisition of extracellular genes and intracellular mutational events were identified as the processes that led to the emergence and spread of resistant genotypes. Oxford Nanopore Technologies sequencing helped decipher the complex molecular epidemiology of resistant Campylobacter dissemination.
SCIENTIFIC REPORTS
(2023)
Article
Multidisciplinary Sciences
Yuli Melisa Sierra-Arguello, Gustavo Perdoncini, Laura Beatriz Rodrigues, Luciana Ruschel dos Santos, Karen Apellanis Borges, Thales Quedi Furian, Carlos Tadeu Pippi Salle, Hamilton Luiz de Souza Moraes, Marcos Jose Pereira Gomes, Vladimir Pinheiro do Nascimento
Summary: Campylobacter jejuni is a common cause of foodborne illnesses worldwide, with limited research on strains isolated from Latin-American countries. This study in Southern Brazil identified 140 C. jejuni strains from various poultry sources and analyzed their virulence markers, providing insight into factors related to the occurrence of this pathogen and their epidemiology. The PCR analysis revealed 25 different virulence profiles, with certain combinations of virulence genes being more common among the strains.
SCIENTIFIC REPORTS
(2021)
Article
Cell Biology
Lucia Casadei, Adarsh Choudhury, Patricia Sarchet, Prashanth Mohana Sundaram, Gonzalo Lopez, Danielle Braggio, Gita Balakirsky, Raphael Pollock, Shaurya Prakash
Summary: This study presents a resource-efficient approach using a micro-nanofluidic device for isolating and capturing liposarcoma-derived extracellular vesicles, leading to a significant advance in retrieval of critical liposarcoma-relevant extracellular vesicle cargo compared to state-of-the-art methods.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Review
Food Science & Technology
Gabriele Rocchetti, Rosa Perez Gregorio, Jose M. Lorenzo, Francisco J. Barba, Paula Garcia Oliveira, Miguel A. Prieto, Jesus Simal-Gandara, Juana Mosele, Maria-Jose Motilva, Merve Tomas, Vania Patrone, Esra Capanoglu, Luigi Lucini
Summary: Scientific evidence suggests the health benefits of phenolic compounds and dietary fiber, and these two components may interact with each other. Food processing can affect these interactions. Fiber-bound phenolic compounds undergo transformation in the large intestine and can impact gut microbiota. However, there is limited information on the effects of fiber-bound phenolics on gut microbiota, and further research is needed in this area.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2022)
Review
Oncology
Soumya R. Sharma, Nadia E. Paonessa, Lucia Casadei, Fernanda Costas De Faria, Raphael E. Pollock, Valerie Grignol
Summary: Soft tissue sarcomas are a heterogeneous group of tumors that arise from mesenchymal tissue, with recent research providing new insight into potential biomarkers at the genomic, proteomic, and immunological levels. The identification of biomarkers can improve diagnosis, prognosis, prediction of recurrence, and treatment response in STS.
JOURNAL OF SURGICAL ONCOLOGY
(2022)
Article
Pharmacology & Pharmacy
Mattia Tiboni, Enas Elmowafy, Marwa O. El-Derany, Serena Benedetti, Raffaella Campana, Michele Verboni, Lucia Potenza, Francesco Palma, Barbara Citterio, Maurizio Sisti, Andrea Duranti, Simone Lucarini, Mahmoud E. Soliman, Luca Casettari
Summary: Researchers have been exploring innovative green materials made from renewable natural substances, such as chitosans and biosurfactants, for formulation needs. These biopolymers possess biocompatibility, biodegradability, functionality, and cost-effectiveness. They have shown potential for wound dressing to aid in physiological wound healing, which involves oxidative damage, inflammation, and infection risks. The study synthesized different sugar esters in combination with chitosan and sucrose laurate and evaluated their cytocompatibility, anti-inflammatory, antioxidant, and antibacterial activities. The combination showed superior wound healing prospects and restoration of the Wnt/beta-catenin signaling pathway.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2022)
Article
Infectious Diseases
Raffaella Campana, Mattia Tiboni, Filippo Maggi, Loredana Cappellacci, Kevin Cianfaglione, Mohammad Reza Morshedloo, Emanuela Frangipani, Luca Casettari
Summary: The antimicrobial activity of several essential oils and their related microemulsions was investigated. It was found that cumin, sea fennel, and anise essential oils were effective against various microorganisms. The antimicrobial activity was further enhanced when the essential oils were formulated into microemulsions, resulting in lower minimum inhibitory concentration and minimum bactericidal concentration values.
Article
Chemistry, Medicinal
Michele Verboni, Serena Benedetti, Raffaella Campana, Francesco Palma, Lucia Potenza, Maurizio Sisti, Andrea Duranti, Simone Lucarini
Summary: This study investigates a new glycolipid compound, URB1418, which exhibits antifungal and anti-inflammatory properties. It is also more stable and cost-effective to produce compared to reference compounds, making it a promising candidate for various applications.
Article
Biochemistry & Molecular Biology
Jotham Suez, Yotam Cohen, Rafael Valdes-Mas, Uria Mor, Mally Dori-Bachash, Sara Federici, Niv Zmora, Avner Leshem, Melina Heinemann, Raquel Linevsky, Maya Zur, Rotem Ben-Zeev Brik, Aurelie Bukimer, Shimrit Eliyahu-Miller, Alona Metz, Ruthy Fischbein, Olga Sharov, Sergey Malitsky, Maxim Itkin, Noa Stettner, Alon Harmelin, Hagit Shapiro, Christoph K. Stein-Thoeringer, Eran Segal, Eran Elinav
Summary: This study found that the consumption of non-nutritive sweeteners (NNS) may alter the microbiome and glycemic responses in humans. Different NNS had distinct effects on the microbiome and glycemic responses, with saccharin and sucralose significantly impairing glycemic responses. Additionally, when NNS were supplemented to mice, the glycemic responses reflected those in respective human donors and were influenced by microbial signals.
Article
Biochemistry & Molecular Biology
Raffaella Campana, Luigia Sabatini, Luca Giorgi, Giulia Pettinari, Laura Valentini, Pietro Gobbi
Summary: This study investigated the susceptibility of synthetic polymers to microorganisms and found that they are prone to microbial attack regardless of the biocide treatment. Penicillium citrinum showed the greatest adaptation ability among the examined resins, while P. aeruginosa was visible in all the resins regardless of the presence of biocide.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Raffaella Campana, Francesco Palma, Maurizio Sisti
Summary: This research investigated the growth characteristics of Zalaria obscura LS31012019 in different media, temperatures, and carbon sources. The results showed that Z. obscura LS31012019 can grow well in various conditions and utilize carbon sources other than glucose and sucrose.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Mycology
Raffaella Campana, Fabiana Fanelli, Maurizio Sisti
Summary: The role of melanin in black-fungi, specifically in Aureobasidium pullulans and Zalaria obscura, was investigated in this study. The results showed that melanin production contributed to increased tolerance to high temperature and UV-C radiation, while non-melanized cells were more susceptible to antifungals and essential oils. This research provides novel insights into the biological role of melanin in black-fungi and enhances our understanding of their environmental adaptive ability.
Article
Biochemistry & Molecular Biology
Mariele Montanari, Michele Guescini, Ozan Gundogdu, Francesca Luchetti, Paola Lanuti, Caterina Ciacci, Sabrina Burattini, Raffaella Campana, Claudio Ortolani, Stefano Papa, Barbara Canonico
Summary: Cytolethal distending toxin (CDT) is an important virulence factor produced by Gram-negative pathogenic bacteria. It targets the endo-lysosomal compartment and exploits unconventional secretion (EV release) to cause cell cycle arrest and cell death. Extracellular vesicles (EVs) play crucial roles in cellular communication and can also spread molecules during infections to alter immune responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Medicinal
Michele Verboni, Maurizio Sisti, Raffaella Campana, Serena Benedetti, Francesco Palma, Lucia Potenza, Simone Lucarini, Andrea Duranti
Summary: A library of 6-O-sucrose monoester surfactants was synthesized and tested for their antimicrobial activities. The synthetic procedure, a modified Mitsunobu reaction, showed improved results compared to the literature. Most of these glycolipids exhibited satisfactory antifungal activities, with sucrose palmitoleate (URB1537) showing good activity against Candida albicans ATCC 10231, Fusarium spp., and Aspergillus fumigatus IDRAH01. URB1537 was further characterized for its radical scavenging, anti-inflammatory, and biocompatibility properties, demonstrating control of inflammatory response and safety.
Article
Food Science & Technology
Rebecca Ghisolfi, Francesca Bandini, Filippo Vaccari, Gabriele Bellotti, Cristian Bortolini, Vania Patrone, Edoardo Puglisi, Lorenzo Morelli
Summary: This study used high-throughput sequencing to assess the impact of box, ground, and jute fermentation methods on bacterial and fungal communities. The results showed noticeable differences between the three methods, with box fermentation having higher bacterial species diversity and ground processing resulting in a wider fungal community. The box method was found to be preferable due to its limited microbial diversity and the presence of microorganisms that ensure good fermentation. The study provided a comprehensive exploration of the microbiota of differently treated cocoa beans and improved understanding of the technological processes for obtaining a standardized end-product.
Article
Food Science & Technology
Walter Mancino, Paola Carnevali, Valeria Terzi, Pascual Garcia Perez, Leilei Zhang, Gianluca Giuberti, Lorenzo Morelli, Vania Patrone, Luigi Lucini
Summary: A multi-omics approach was used to investigate the impact of lactic acid fermentation and seed germination on the composition and physicochemical properties of rye doughs. The study found that fermentation increased acidity and dough rise, regardless of the flour used. Germination had a strong impact on the bacterial community profile of sprouted rye flour, with different types of bacteria present in doughs made with germinated and native rye.
Article
Health Care Sciences & Services
L. Sabatini, M. Sisti, R. Campana
Summary: This study is the first to describe the distribution of Legionella spp. in non-hospital facilities in the province of Pesaro-Urbino and highlights a potential risk for susceptible populations. Regular and constant control measures to prevent microbiological risk from legionellosis, especially in facilities housing the elderly, are recommended.
ANNALI DI IGIENE MEDICINA PREVENTIVA E DI COMUNITA
(2022)