Article
Microbiology
Tomohiro Morohoshi, Akinori Oshima, Xiaonan Xie, Nobutaka Someya
Summary: Our study demonstrates that the PsyI/PsyR quorum-sensing systems in Pseudomonas syringae strains are genetically and functionally diverse, with diversity being linked to phylogenetic and pathovar classifications.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Microbiology
Nicole E. Smalley, Amy L. Schaefer, Kyle L. Asfahl, Crystal Perez, E. Peter Greenberg, Ajai A. Dandekar
Summary: In the bacterium Pseudomonas aeruginosa, quorum sensing (QS) can activate the expression of dozens to hundreds of genes. In this study, the researchers investigated how the size and content of the QS regulon can evolve during long-term growth of P. aeruginosa. They found that the QS regulon can undergo a reductive adaptation in response to continuous QS-dependent growth. These findings provide insights into the strain-to-strain variability in the size and content of the P. aeruginosa QS regulon.
Article
Microbiology
Nicole E. Smalley, Amy L. Schaefer, Kyle L. Asfahl, Crystal Perez, E. Peter Greenberg, Ajai A. Dandekar
Summary: This study investigates the evolution of the QS regulon in Pseudomonas aeruginosa during long-term growth when cells require some but not all the functions activated by QS. The research reveals that the QS-regulon in P. aeruginosa can undergo a reductive adaptation in response to continuous QS-dependent growth, providing insights into the variability in the size and content of the QS regulon in different strains.
Article
Microbiology
Jose Carlos Reina, Pedro Perez, Inmaculada Llamas
Summary: Some strains isolated from marine invertebrates have been found to possess the ability to degrade bacterial communication molecules (AHLs), thereby reducing pathogenic virulence and potentially being applied in biotechnological processes.
Article
Immunology
Melanie Gonzales, Laure Plener, Jean Armengaud, Nicholas Armstrong, Eric Chabriere, David Daude
Summary: This study demonstrates the importance of Quorum sensing in Burkholderia thailandensis using an enzyme-based quorum quenching strategy. The disruption of quorum sensing greatly affects bacterial behavior, including motility, proteolytic activity, and antimicrobial molecule production. Furthermore, the study shows that quorum sensing disruption significantly enhances the bactericidal activity against fungi and yeast. These findings are of prime interest for understanding the virulence of Burkholderia species and developing alternative treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Plant Sciences
Manoj Pun, Netaly Khazanov, Ortal Galsurker, Michal Weitman, Zohar Kerem, Hanoch Senderowitz, Iris Yedidia
Summary: Phloretin from apple inhibits the growth and virulence of Pectobacterium brasiliense (Pb1692), an emerging soft-rot pathogen of potato, by reducing motility, biofilm formation, enzyme secretion, AHL signaling molecule production, and infection. The compound inhibits ExpI activity and downregulates virulence-related genes associated with quorum sensing. Docking simulations suggest a likely mode of action for phloretin's inhibition of virulence.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Engineering, Environmental
Xueping Wang, Deshuang Yu, Guanghui Chen, Chengju Liu, Ao Xu, Zhihao Tang
Summary: This paper discusses the bacterial communication mechanism based on QS and the environmental factors affecting QS, compares QS inhibition strategies, and explores the role and prospects of QQ in long-term operation systems.
WATER ENVIRONMENT RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Sania Sahreen, Hamid Mukhtar, Kalman Imre, Adriana Morar, Viorel Herman, Sundas Sharif
Summary: This review discusses the role of quorum sensing (QS) in biofilm formation and its importance in biological wastewater treatment. The different types of autoinducers and their mechanisms are explained, along with the methods of QS regulation and inhibition. The potential applications of QS-regulated biofilms in wastewater treatment are also summarized. The article concludes with an analysis of knowledge gaps in current research and proposed future study requirements.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Rafaela A. Santos, Marta Monteiro, Fabio Rangel, Russell Jerusik, Maria J. Saavedra, Antonio Paulo Carvalho, Aires Oliva-Teles, Claudia R. Serra
Summary: This study characterized the QQ potential of 200 Bacillus spp. isolated from the gut of different aquaculture fish species to suppress fish pathogens QS, and identified three Bacillus spp. capable of extracellularly quenching aquaculture pathogen communication, showing promising biocontrol potential against aquaculture bacterial diseases.
Article
Microbiology
Marion Urvoy, Raphael Lami, Catherine Dreanno, Daniel Delmas, Stephane L'Helguen, Claire Labry
Summary: The study found that regulating AHLs in marine planktonic assemblages can impact both hydrolytic activities and bacterial community composition. Specific enzymatic activities were impacted as early as 6 hours after AHL amendments in both experiments; after 48 hours, the BCC was significantly affected and correlated with the expression of hydrolytic activities, indicating changes in hydrolytic intensities may drive changes in BCC.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tomohiro Morohoshi, Kanako Nameki, Nobutaka Someya
Summary: The complete genome sequences of 3 strains of Erwinia rhapontici were presented, revealing a variety of luxl/luxR gene pairs involved in acylhomoserine lactone biosynthesis and reception. A large-scale insertion sequence was observed in the native plasmid of MAFF 311154, which contained eraI3/eraR3 gene pair enabling the production of acylhomoserine lactone.
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2021)
Review
Chemistry, Medicinal
Jed F. F. Fisher, Shahriar Mobashery
Summary: This essay discusses the discovery of beta-lactam antibiotics and their transformation of modern medicine, while also pointing out the scarcity of beta-lactam compounds in marine organisms and their potential similarity to quorum sensing molecules. The author proposes the exploration of this similarity to discover new structures and enzyme targets for interesting biological activities.
Article
Biotechnology & Applied Microbiology
Aparna Anil Singh, Anil Kumar Singh, Anuradha Nerurkar
Summary: Genus Pectobacterium is a major cause of soft rot diseases in fruits and vegetables globally, and there is a need for alternative ecofriendly and economical strategies. Bacillus sp. OA10 extract can effectively inhibit the pathogenicity of PccBR1, potentially by disrupting the QS pathways.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Review
Polymer Science
Moupriya Nag, Dibyajit Lahiri, Dipro Mukherjee, Ritwik Banerjee, Sayantani Garai, Tanmay Sarkar, Sujay Ghosh, Ankita Dey, Sougata Ghosh, Smaranika Pattnaik, Hisham Atan Edinur, Zulhisyam Abdul Kari, Siddhartha Pati, Rina Rani Ray
Summary: Chitosan and its nano materials show potential in combating drug resistance by disrupting quorum sensing in microbial biofilms, offering a solution for treating chronic infections and preparing medical devices. Through interaction with amino groups on microorganisms, they disrupt membrane permeability and induce cell death.
Article
Engineering, Environmental
Chunxiao Dai, Yuanyuan Qu, Weize Wu, Shuzhen Li, Zhuo Chen, Shengyang Lian, Jiawei Jing
Summary: This study established a QS-related protein database and an automatic annotation pipeline for rapid and accurate annotation and classification of QS-related sequences in aquatic environments. It provides a useful tool for understanding bacterial communication in water environments.
Review
Fisheries
Ngoc Tuan Tran, Wei Yang, Xuan Truong Nguyen, Ming Zhang, Hongyu Ma, Huaiping Zheng, Yueling Zhang, Kok-Gan Chan, Shengkang Li
Summary: Heat-killed probiotics offer benefits to aquatic animals without negatively impacting the aquaculture environment. This review discusses their sources, safety, effectiveness, and application methods in aquaculture, as well as their positive effects on intestinal structure, growth performance, stress tolerance, pathogen protection, and host immunity.Future research directions on the application of heat-killed probiotics in aquaculture are proposed.
Article
Biology
Kah-Ooi Chua, Iqra Fatima, Yin Yin Lau, Kar Wai Hong, Wai-Fong Yin, Andrei Mardaryev, Kok-Gan Chan, Chien-Yi Chang
Summary: This study characterized the bacterial microbiome of fecal samples collected from the toilet chamber of a laboratory naked mole rat colony using 16S rRNA gene amplicon sequencing. The results revealed that the dominant taxa in the bacterial microbiome of the toilet chamber were Firmicutes and Bacteroidetes, and their relative abundance varied significantly between time points.
BMC RESEARCH NOTES
(2022)
Article
Microbiology
Kah-Ooi Chua, Yvonne Jing Mei Liew, Wah-Seng See-Too, Jia-Yi Tan, Hoi-Sen Yong, Wai-Fong Yin, Kok-Gan Chan
Summary: In this study, a novel bacterium named Formicincola oecophyllae gen. nov. sp. nov. was isolated from the gut of weaver ant. It belongs to the family Acetobacteraceae and has distinct characteristics in morphology, physiology, and gene sequence compared to its closest relatives.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Joanna Xuan Hui Goh, Loh Teng-Hern Tan, Jodi Woan-Fei Law, Kooi-Yeong Khaw, Nurul-Syakima Ab Mutalib, Ya-Wen He, Bey-Hing Goh, Kok-Gan Chan, Learn-Han Lee, Vengadesh Letchumanan
Summary: The increasing prevalence of carbapenem resistance is a growing concern, and it has been discovered that environmental microbial populations could be indirectly contributing to this resistance. This study aims to explore the carbapenem resistance profile of Vibrio species in various settings and identify the transmission pathways for carbapenem resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Yvonne Jing Mei Liew, Kah-Ooi Chua, Hoi-Sen Yong, Sze-Looi Song, Kok-Gan Chan
Summary: This study sequenced and compared the chloroplast genome of Boesenbergia rotunda, identifying highly variable regions that could be used as molecular markers and conducting phylogenetic analysis using shared protein-coding genes, revealing the phylogenetic position of B. rotunda within the Zingiberaceae family.
BRAZILIAN JOURNAL OF BOTANY
(2022)
Article
Biotechnology & Applied Microbiology
Jean-Frederic Dubern, Manuel Romero, Anne Mai-Prochnow, Marco Messina, Eleftheria Trampari, Hardeep Naghra-van Gijzel, Kok-Gan Chan, Alessandro M. Carabelli, Nicolas Barraud, James Lazenby, Ye Chen, Shaun Robertson, Jacob G. Malone, Paul Williams, Stephan Heeb, Miguel Camara
Summary: Pseudomonas aeruginosa uses multiple protein regulators, including ToxR, to control the production of virulence factors and adapt to different environments. This study reveals the previously unknown role of ToxR in surface-associated motility and its impact on the Rhl quorum sensing system and biofilm formation in P. aeruginosa.
NPJ BIOFILMS AND MICROBIOMES
(2022)
Article
Microbiology
Paul Norton, Kah-Ooi Chua, Maria Katsikogianni, M. Julie Thornton, Kok-Gan Chan, Chien-Yi Chang
Summary: This study presents the draft genome sequence of laboratory strain Staphylococcus aureus NCTC 6571-UB, derived from S. aureus NCTC 6571, to provide insights into genome dynamics and acquired resistance genes for penicillin G, trimethoprim, and sulfamethoxazole resistance.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Microbiology
Kah-Ooi Chua, Paul Norton, Pavlos Trus, Maria Katsikogianni, M. Julie Thornton, Kok-Gan Chan, Chien-Yi Chang
Summary: This study reports the draft genome sequence and antibiotic susceptibility of Pseudomonas aeruginosa strain PAO1-UB, shedding light on the genome dynamics and antibiotic resistance genes in PAO1 sublines.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2022)
Article
Virology
Kok Keng Tee, Po Qhuan Chan, Alson Mun-Khin Loh, Sarbhan Singh, Chee How Teo, Thevambiga Iyadorai, Jack Bee Chook, Kim Tien Ng, Yutaka Takebe, Kok Gan Chan, I-Ching Sam, Kenny Voon
Summary: Pteropine orthoreovirus (PRV), a bat-borne virus, is associated with acute respiratory infections (ARI) in humans. Among 632 urban outpatients, 2.2% tested positive for PRV. Symptoms included cough, sore throat, and muscle ache, with minimal fever. Genetic analysis revealed the continuous evolution of PRV through reassortment among human, bat, and non-human primate hosts, emphasizing the importance of monitoring its prevalence and diversity.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Biochemistry & Molecular Biology
Aurora Garcia-Berro, Venkat Talla, Roger Vila, Hong Kar Wai, Daria Shipilina, Kok Gan Chan, Naomi E. Pierce, Niclas Backstrom, Gerard Talavera
Summary: This study investigates the heterozygosity and population size dynamics of migratory insects using genomic data. The findings show that migratory species have higher levels of genome-wide heterozygosity than nonmigratory species. The study also reveals that migratory Lepidoptera maintain overall genetic diversity and demographic stability despite the risks associated with migration and local environmental fluctuations.
Article
Public, Environmental & Occupational Health
Ajaree Rayanakorn, Wasan Katip, Zanfina Ademi, Kok-Gan Chan
Summary: This study aims to estimate the direct medical costs associated with S.suis treatment in Thailand and identify key drivers affecting high treatment costs. The study found that infective endocarditis, Glasgow Coma Scale, length of stay, and bicarbonate level were significant predictors associated with high total treatment costs. Therefore, early diagnosis and timely treatment are crucial in alleviating long-term complications and high healthcare expenditures.
Review
Biochemistry & Molecular Biology
Bingpeng Lin, Hooi Leng Ser, Lijing Wang, Jiang Li, Kok-Gan Chan, Learn-Han Lee, Loh Teng-Hern Tan
Summary: This review provides a comprehensive overview of the role of MMP12 in various oral diseases, including periodontitis, temporomandibular joint dysfunction, orthodontic tooth movement, and oral squamous cell carcinoma. Studies have shown the association of MMP12 expression with the pathogenesis of these oral diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Pharmacology & Pharmacy
Kooi Yeong Khaw, Michael R. R. Whittaker, Sanjaya Kuruppu, Kok Gan Chan, Siew Hua Gan, Bey Hing Goh
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Entomology
Hoi-Sen Yong, Sze-Looi Song, Kah-Ooi Chua, Yvonne Jing Mei Liew, I. Wayan Suana, Phaik-Eem Lim, Kok-Gan Chan, Praphathip Eamsobhana
Summary: The mitochondrial genomes of three fruit fly species from the Zeugodacus genus show similarities in gene order and structure, but differences in certain gene features and taxonomic status. More sampling of different taxonomic units is needed to clarify their classification.
ARTHROPOD SYSTEMATICS & PHYLOGENY
(2023)
Review
Food Science & Technology
Wei-Shan Ang, Jodi Woan-Fei Law, Vengadesh Letchumanan, Kar Wai Hong, Sunny Hei Wong, Nurul Syakima Ab Mutalib, Kok-Gan Chan, Learn-Han Lee, Loh Teng-Hern Tan
Summary: A new probiotic, Christensenella minuta, was discovered in 2012 from healthy human stool. It is a subdominant commensal bacterium with highly heritable properties and has mutual interactions with other heritable microbiomes. Its relative abundance is positively correlated with the lean host phenotype. Various studies have shown its potential in managing metabolic diseases such as obesity, inflammatory bowel disease, and type 2 diabetes.
