Article
Microbiology
Annalisa Chianese, Carla Zannella, Francesca Palma, Laura Di Clemente, Alessandra Monti, Nunzianna Doti, Anna De Filippis, Massimiliano Galdiero
Summary: Emerging viruses pose a global public health challenge and early action is needed to control their spread. Frog-derived peptides RV-23 and AR-23 demonstrated strong antiviral activity against sandfly fever Naples virus by targeting viral particles and blocking virus-cell interaction.
Article
Infectious Diseases
Maria Ortuno, Clara Munoz, Tatiana Spitzova, Petra Sumova, Maria Asuncion Iborra, Pedro Perez-Cutillas, Nazli Ayhan, Remi N. Charrel, Petr Volf, Eduardo Berriatua
Summary: In a study conducted in Murcia Region, southeast Spain, the prevalence of antibodies against Phlebotomus perniciosus sandfly salivary gland homogenate (SGH) and recombinant protein rSP03B was found to be 69% and 88%, respectively. The prevalence of Toscana virus (TOSV), Sandfly Fever Sicilian virus (SFSV), and Leishmania infections were 26%, 0%, and 1%, respectively. The study also questioned the diagnostic value of rSP03B as a marker for these infections in humans.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Virology
Ceylan Polat, Nazli Ayhan, Mehmet Bakir Saygan, Sevilay Karahan, Remi Charrel, Koray Erguenay
Summary: The study found widespread exposure to distinct pathogenic phleboviruses, particularly Toscana virus, in the screened population in Turkey. These findings highlight the need for improved diagnostic testing and serological screening efforts.
Article
Virology
Nazli Ayhan, Marc Lopez-Roig, Abir Monastiri, Remi N. Charrel, Jordi Serra-Cobo
Summary: A study tested 329 bats from eight species collected in southern Spain for neutralizing antibodies specific to TOSV and SFSV, finding bats are not likely major players in the natural cycle of these sandfly-borne viruses; large breeding colonies can be used as sentinels for virus surveillance in specific locations.
Article
Virology
Qinyan Wang, Shihong Fu, Jingxia Cheng, Xiuyan Xu, Jing Wang, Bin Wu, Xiaodong Tian, Yan Li, Ying He, Fan Li, Kai Nie, Songtao Xu, Bin Wang, Huanyu Wang, Xiaoqing Lu, Guodong Liang
Summary: Wuxiang virus (WUXV) was re-isolated from wild sandflies in Yangquan County, China, showing the ability to infect humans and animals. This virus has a relatively wide geographical distribution in China, highlighting the importance of investigation and study on its infection status in humans and animals from a public health perspective.
Article
Immunology
Lison Laroche, Frederic Jourdain, Nazli Ayhan, Anne-Laure Banuls, Remi Charrel, Jorian Prudhomme
Summary: Toscana virus (TOSV) is an emerging pathogen in the Mediterranean area with neuroinvasive properties in its severe form. Basic knowledge about TOSV biology is limited, prompting researchers to conduct a systematic review to estimate its incubation period, which was found to be 12.1 days.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Public, Environmental & Occupational Health
Yue Li, Yu-Na Wang, Feng Tian, Xiao-Long Zhang, Jing-Tao Zhang, Shuang Li, Hao Li, Xiao-Ai Zhang, Wei Liu
Summary: In this study, KARV virus was detected in Rhombomys opimus in China for the first time, expanding the geographic scope and reservoir types known for KARV. The findings highlight the potential zoonotic transmission of KARV in humans.
Article
Clinical Neurology
R. Dersch, A. Sophocleous, D. Cadar, P. Emmerich, J. Schmidt-Chanasit, S. Rauer
Summary: The study identified cases of TOSV-neuroinvasive disease in a German cohort of patients with meningoencephalitis, suggesting possible autochthonous infections without recent travel history to endemic areas. These findings imply the need to consider TOSV in patients with meningoencephalitis in Germany.
Article
Virology
Khalil Dachraoui, Ifhem Chelbi, Mourad Ben Said, Raja Ben Osman, Saifedine Cherni, Remi Charrel, Elyes Zhioua
Summary: The study investigated the transmission dynamics of Punique virus (PUNV) in Tunisia and found that Phlebotomus perniciosus is the main vector of PUNV. The research provided evidence of intensive circulation of PUNV in sandflies and hosts, reaching a peak during the autumn season.
Article
Microbiology
Nariman Shahhosseini, Sarah-Jo Paquette, Mohammad Hassan Kayedi, Mohammad Reza Abaei, Mohammad Mehdi Sedaghat
Summary: This study provides new insights into the molecular epidemiology of phleboviruses, specifically Tehran virus (TEHV), sandfly fever Sicilian viruses (SFSVs), and Karimabad viruses (KARVs) in Iran.
Review
Virology
Benedetta Rossi, Filippo Barreca, Domenico Benvenuto, Neva Braccialarghe, Laura Campogiani, Alessandra Lodi, Camilla Aguglia, Rosario Alessandro Cavasio, Maria Laura Giacalone, Dimitra Kontogiannis, Martina Moccione, Vincenzo Malagnino, Massimo Andreoni, Loredana Sarmati, Marco Iannetta
Summary: This review summarizes the epidemiology of arboviral infection in Italy over the past 10 years, including both endemic and imported infections, vector distribution, and the impact of climate change on vector ecology. Strengthening surveillance systems at a national and international level is highly recommended to prepare for potential threats from arbovirus diffusion.
Article
Virology
Natalia S. S. Barbosa, Juan O. O. Concha, Luis L. P. daSilva, Colin M. M. Crump, Stephen C. C. Graham
Summary: Oropouche virus causes a debilitating illness called Oropouche fever, which is common in South America. The virus has the ability to reassort its genome and has caused multiple epidemics in the region over the last 50 years, posing a significant threat to public health. The study investigates the cellular determinants and mechanisms involved in Oropouche virus replication and secretion.
JOURNAL OF VIROLOGY
(2023)
Article
Microbiology
Sarah Hulsey Stubbs, Marjorie Cornejo Pontelli, Nischay Mishra, Changhong Zhou, Juliano de Paula Souza, Rosa Maria Mendes Viana, W. Ian Lipkin, David M. Knipe, Eurico Arruda, Sean P. J. Whelan
Summary: Oropouche virus (OROV) infection can lead to severe febrile illness and potentially progress to meningitis or encephalitis. Researchers developed a chimeric vesicular stomatitis virus (VSV) bearing OROV glycoproteins, which elicited a strong neutralizing antibody response in mice and showed potential in reducing viral loads, suggesting targeting OROV glycoproteins could be an effective vaccination strategy against OROV infection.
Review
Virology
Fatima Amaro, Libia Ze-Ze, Maria Joao Alves
Summary: According to ICTV, there are currently 66 known species of phlebovirus, with more than 40 of them being isolated or detected in phlebotomine sandflies. In Portugal, there is limited information about sandfly-borne phleboviruses, and sandfly-borne diseases are often neglected and not considered in diagnoses. This study aims to gather existing information and raise awareness about circulating phleboviruses in the country. Massilia and Alcube phleboviruses have been isolated from sandflies in southern Portugal, and human infections with Toscana and Sicilian phleboviruses have been reported, along with seroprevalence in cats and dogs. Further studies are needed to understand the impact of these viruses on human health and the true importance of recognized pathogens in the country.
Article
Biochemistry & Molecular Biology
Hao Li, Xia-Ming Jiang, Ning Cui, Chun Yuan, Shao-Fei Zhang, Qing-Bin Lu, Zhen-Dong Yang, Qin-Lin Xin, Ya-Bin Song, Xiao-Ai Zhang, Hai-Zhou Liu, Juan Du, Xue-Juan Fan, Lan Yuan, Yi-Mei Yuan, Zhen Wang, Juan Wang, Lan Zhang, Dong-Na Zhang, Zhi-Bo Wang, Ke Dai, Jie-Ying Bai, Zhao-Nian Hao, Hang Fan, Li-Qun Fang, Gengfu Xiao, Yang Yang, Ke Peng, Hong-Quan Wang, Jian-Xiong Li, Lei-Ke Zhang, Wei Liu
Summary: The study showed that T-705 treatment for SFTS can reduce case fatality rate, especially in patients with low viral loads. The T-705-treated group exhibited faster viral clearance, lower incidence of hemorrhagic signs, and quicker recovery from laboratory abnormalities compared to the controls.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Article
Virology
Nazli Ayhan, Marc Lopez-Roig, Abir Monastiri, Remi N. Charrel, Jordi Serra-Cobo
Summary: A study tested 329 bats from eight species collected in southern Spain for neutralizing antibodies specific to TOSV and SFSV, finding bats are not likely major players in the natural cycle of these sandfly-borne viruses; large breeding colonies can be used as sentinels for virus surveillance in specific locations.
Editorial Material
Virology
Remi N. Charrel
Article
Microbiology
Laurence Thirion, Laura Pezzi, Irene Pedrosa-Corral, Sara Sanbonmatsu-Gamez, Xavier De Lamballerie, Alessandra Falchi, Mercedes Perez-Ruiz, Remi N. Charrel
Summary: In this study, three real-time RT-qPCR assays for detecting TOSV RNA were evaluated and combined to create a multi-region assay named Trio TOSV RT-qPCR. The Trio TOSV RT-qPCR assay showed equal or better performance in specificity and sensitivity compared to the three monoplex assays, indicating its potential for both research and diagnostic purposes.
Article
Virology
Lea Luciani, Denis Mongin, Laetitia Ninove, Antoine Nougairede, Kevin Bardy, Celine Gazin, Remi N. Charrel, Christine Zandotti
Summary: The evaluation of NeuMoDx™ in measuring CMV viral load showed that it is reliable and allows for rapid measurement, aiding in effective patient management. Despite initial differences in viral load measurement, long-term use of NeuMoDx™ compared to the classical technique R-GENE(R) did not demonstrate significant differences in patient management.
Article
Microbiology
Francis Simo-Fouda, Laurence Thirion, Antoine Nougairede, Lea Luciani, Jean-Selim Driouich, Paul Remi Petit, Pascal Delaunay, Remi N. Charrel
Summary: In the South-East of France, Bufavirus (BuV) circulates with a relatively high rate in stool samples, especially in men, adults, and patients from nephrology and infectious disease departments. In contrast, human parvovirus 4 (PARV4) was rarely detected in stool samples in this area.
Article
Microbiology
Marie Pouquet, Dorine Decarreaux, Pol Prevot-Monsacre, Corentin Herve, Andreas Werner, Brigitte Grosgogeat, Helene Blanche, Pascaline Rabiega, Julien Laupie, Fabienne Kochert, Nathalie Abraham, Jean-Marc Sebaoun, Xavier de Lamballerie, Remi Charrel, Cecile Souty, Ibrahima Camara, Jeanne Pergeline, Harold Noel, Caroline Guerrisi, Sylvie van der Werf, Fabrice Carrat, Thomas Hanslik, Thierry Blanchon, Alessandra Falchi
Summary: This study focuses on the seroprevalence of IgG antibodies against SARS-CoV-2 in primary health care workers and their household contacts in metropolitan France, achieved through vaccination and/or natural protection. It will be conducted in two phases using ELISA and virus neutralization tests to estimate seroprevalence.
Article
Microbiology
Francis Simo-Fouda, Laetitia Ninove, Lea Luciani, Christine Zandotti, Celine Gazin, Remi N. Charrel, Antoine Nougairede
Summary: The study retrospectively evaluated the rotavirus and adenovirus ICT routinely used in the virology laboratory at the University Hospital of Marseille, France. The results showed that the ICT had high sensitivity and specificity for rotavirus detection, but low sensitivity for adenovirus detection, indicating that a PCR-based assay is better suited for adenovirus detection in stools.
Article
Infectious Diseases
Maria Ortuno, Clara Munoz, Tatiana Spitzova, Petra Sumova, Maria Asuncion Iborra, Pedro Perez-Cutillas, Nazli Ayhan, Remi N. Charrel, Petr Volf, Eduardo Berriatua
Summary: In a study conducted in Murcia Region, southeast Spain, the prevalence of antibodies against Phlebotomus perniciosus sandfly salivary gland homogenate (SGH) and recombinant protein rSP03B was found to be 69% and 88%, respectively. The prevalence of Toscana virus (TOSV), Sandfly Fever Sicilian virus (SFSV), and Leishmania infections were 26%, 0%, and 1%, respectively. The study also questioned the diagnostic value of rSP03B as a marker for these infections in humans.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Virology
Nazli Ayhan, Aissam Hachid, Laurence Thirion, Kamel Eddine Benallal, Laura Pezzi, Fayez Ahmed Khardine, Chahrazed Benbetka, Sihem Benbetka, Zoubir Harrat, Remi Charrel
Summary: Sindbis virus (SINV), a zoonotic alphavirus, has been identified as a cause of human diseases in Africa, Europe, Asia, and Australia. In this study, SINV was detected and isolated from mosquitoes in Algeria, and genetic analysis revealed its similarity to a strain found in Kenya.
Article
Virology
Dorine Decarreaux, Marie Pouquet, Cecile Souty, Ana-Maria Vilcu, Pol Prevot-Monsacre, Toscane Fourie, Paola Mariela Saba Villarroel, Stephane Priet, Helene Blanche, Jean-Marc Sebaoun, Jean-Francois Deleuze, Clement Turbelin, Andreas Werner, Fabienne Kochert, Brigitte Grosgogeat, Pascaline Rabiega, Julien Laupie, Nathalie Abraham, Caroline Guerrisi, Harold Noel, Sylvie van der Werf, Fabrice Carrat, Thomas Hanslik, Remi Charrel, Xavier de Lamballerie, Thierry Blanchon, Alessandra Falchi
Summary: This study aimed to investigate the immune response and neutralizing activity against SARS-CoV-2 among primary health care workers (PHCW) in France and assess the association between neutralizing activity and various factors, including the COVID-19 vaccination scheme. The study found that individuals with previous SARS-CoV-2 infection were more likely to have neutralizing antibodies, regardless of vaccination status. Among those vaccinated with two doses, the time since vaccination was associated with the detection of neutralizing antibodies.
Article
Virology
Khalil Dachraoui, Ifhem Chelbi, Mourad Ben Said, Raja Ben Osman, Saifedine Cherni, Remi Charrel, Elyes Zhioua
Summary: The study investigated the transmission dynamics of Punique virus (PUNV) in Tunisia and found that Phlebotomus perniciosus is the main vector of PUNV. The research provided evidence of intensive circulation of PUNV in sandflies and hosts, reaching a peak during the autumn season.
Article
Environmental Sciences
Dorine Decarreaux, Julie Sevila, Shirley Masse, Lisandru Capai, Toscane Fourie, Paola Mariela Saba Villarroel, Abdennour Amroun, Elif Nurtop, Matthieu Vareille, Marie Pouquet, Thierry Blanchon, Xavier de Lamballerie, Remi Charrel, Alessandra Falchi
Summary: This study estimated the seroprevalence of SARS-CoV-2 infection among the staff and students of the University of Corsica during the second wave of the epidemic. The overall seroprevalence was found to be 12.8%, and factors such as residence, public transportation usage, and contact with infected individuals were associated with seropositivity. The main symptoms associated with seropositivity were acute respiratory infection and loss of smell.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Immunology
Tetsuro Ikegami, Eduardo Jurado-Cobena, Cigdem Alkan, Jennifer K. Smith, Lihong Zhang, Birte Kalveram, Terry L. Juelich, Allen T. Esterly, Jahnavi R. Bhaskar, Saravanan Thangamani, Alexander N. Freiberg
Summary: RVax-1, a vaccine candidate with protective epitopes, demonstrates immunogenicity and protective efficacy similar to the existing vaccine, while preventing viral reassortment, making it a potential next-generation vaccine for Rift Valley fever prevention.
Article
Environmental Sciences
Dorine Decarreaux, Julie Sevila, Shirley Masse, Lisandru Capai, Toscane Fourie, Paola Mariela Saba Villarroel, Abdennour Amroun, Elif Nurtop, Matthieu Vareille, Thierry Blanchon, Xavier de Lamballerie, Remi Charrel, Alessandra Falchi
Summary: This study investigated the humoral immune response to SARS-CoV-2 in a French adult population. The results showed that single infection does not provide effective protection, and vaccination significantly affects antibody levels and neutralizing activity.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Virology
Khalil Dachraoui, Ifhem Chelbi, Imen Labidi, Raja Ben Osman, Aida Sayadi, Mourad Ben Said, Saifedine Cherni, Mohammed Abdo Saghir Abbas, Remi Charrel, Elyes Zhioua
Summary: This study investigated the role of dogs as reservoir hosts for Toscana virus (TOSV) and Leishmania infantum infections. Dogs exposed to sandfly bites were examined for TOSV and L. infantum infections, and the sandflies were screened for the presence of these pathogens. The results showed that dogs with zoonotic visceral leishmaniasis (ZVL) can serve as reservoirs for both TOSV and L. infantum, and can transmit TOSV to sandfly vectors in natural settings.