Article
Fisheries
Joao F. Romero, Ian A. Gardner, Larry Hammell, David Groman, Daryl Whelan, Nicole O'Brien, Leighanne J. Hawkins, Holly Burnley, Krishna Thakur
Summary: This study describes the epidemiology and genetic variants of an ISAV outbreak in southern Newfoundland, Canada in 2020 and 2021. Multiple ISAV variants were identified, including concurrent detection of different variants in one fish. Understanding the disease dynamics and changes is crucial for disease management plans.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Yan Zhou, Tian-Xiu Qiu, Huan Wang, Ling Hu, Lei Liu, Jiong Chen
Summary: In recent years, the exploration of natural compounds with immunostimulatory and antiviral activities in aquaculture research has gained attention. Rhein, with its wide distribution and multiple biological activities, has shown potential as an immunomodulator in the battle against SVCV infections. It exhibits significant antiviral effects in vitro by blocking viral internalization and has demonstrated efficacy in vivo by increasing the expression of antiviral-related genes and reducing viral load. This research highlights the viability of rhein for future commercial deployment.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
O. Gervais, A. Barria, A. Papadopoulou, R. L. Gratacap, B. Hillestad, A. E. Tinch, S. A. M. Martin, D. Robledo, R. D. Houston
Summary: The study found that the heritability of ISAV resistance is moderate with a polygenic architecture, but a significant quantitative trait locus was detected on chromosome 13. A mild up-regulation of the interferon pathway characterizes the response to the virus in heart samples from this population of Atlantic salmon, and candidate genes showing differential expression between samples with high and low breeding values for resistance were identified.
Article
Fisheries
Chen Li, Wenyan Zhang, Lin Shi, Yuanan Lu, Jing Ye, Xueqin Liu
Summary: In this study, mass spectrometry (MS) and tandem affinity purification (TAP) were used to identify host proteins that interact with the glycoprotein of Spring viremia of carp virus (SVCV). Prohibitin (PHB) was found to have the strongest interaction with SVCV-G protein. Further experiments confirmed that PHB is essential for the binding and entry of SVCV into host cells. Overexpression of PHB increased SVCV attachment, while inhibition of PHB expression reduced binding and entry of SVCV. Treatment with recombinant PHB protein (rPHB) or blocking cell surface PHB inhibited SVCV binding to host cells. In vivo studies showed that treatment with rPHB significantly inhibited SVCV propagation and improved the survival rate of infected zebrafish.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Chen Li, Yunli Zhang, Mengdi Yuan, Wenping Zhu, Chao Pei, Xianliang Zhao, Xianghui Kong
Summary: In this study, a fusion protein combining the glycoprotein (G) of Spring viremia of carp virus (SVCV) and the beta-defensin3 (BD3) of Yellow River carp was created and expressed in E. coli. The fusion protein was used to develop an oral vaccine for Yellow River carp, which showed a higher immune protection rate (77.28%) against SVCV. This study provides guidance for the prevention of SVCV in aquaculture.
Article
Biotechnology & Applied Microbiology
Ophelie Gervais, Carolina Penaloza, Remi Gratacap, Athina Papadopoulou, Mariana Beltran, Neil C. Henderson, Ross D. Houston, Musa A. Hassan, Diego Robledo
Summary: In this study, single-cell RNA sequencing was used to provide a high-dimensional insight into the transcriptional landscape of Atlantic salmon cells during early Infectious Salmon Anaemia Virus (ISAV) infection. The results showed a clear anti-viral response in infected cells, characterized by the upregulation of IFNA2 or IRF2. Additionally, uninfected bystander cells exhibited transcriptional differences, suggesting paracrine signaling from infected cells. This study identified potential key genes involved in the host-virus interaction, which can be targeted in future functional studies to enhance the resistance of Atlantic salmon to ISAV.
Article
Fisheries
Rongrong Liu, Fei Meng, Xin Li, Hua Li, Guiwen Yang, Shijuan Shan
Summary: This study cloned and characterized common carp STING (CcSTING), and found that it plays a critical role in the immune response against RNA viruses. CcSTING is mainly located in the cytoplasm within the endoplasmic reticulum, and has strong expression in organs such as gills and head kidney. Additionally, overexpression of CcSTING can significantly suppress the replication of SVCV in EPC cells, likely through enhancing IFN and NF-κB activity and up-regulating the phosphorylation of TBK1 and p65.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Yan Zhou, Yang Hu, Bin Zhu, Lei Liu, Jiong Chen
Summary: This study demonstrates the antiviral activity of emodin against spring viremia of carp virus (SVCV) through both in vitro and in vivo experiments, suggesting its potential as a commercial antiviral additive in aquaculture.
Article
Immunology
Rongrong Liu, Yan Niu, Yue Qi, Hua Li, Guiwen Yang, Shijuan Shan
Summary: This study investigates the mucosal immune response of common carp to Spring viremia of carp virus (SVCV) infection. Transcriptome analysis identifies differentially expressed genes (DEGs) and two key genes of the RIG-I-like receptor family are further analyzed. Overexpression of these genes enhances immune gene expression and inhibits viral replication. Furthermore, the study reveals an interaction between these two genes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Microbiology
Ping Ouyang, Yu Tao, Wenyan Wei, Qiunan Li, Shuya Liu, Yongqiang Ren, Xiaoli Huang, Defang Chen, Yi Geng
Summary: This study found that infection with spring viremia of carp virus (SVCV) can induce the expression of carp IL-10 and upregulate it through the JAK-STAT, NF-κB, and p38 MAPK pathways.
Article
Biophysics
Maria Elena Tarnok, Fanny Guzman, Luis F. Aguilar
Summary: This study investigated the influence of cholesterol content on membrane fusion facilitated by ISAV fusion peptides. Results showed that cholesterol affected membrane fusion, but the peptides did not require cholesterol in their membranes for fusion in the smallest lipid vesicles (0.2 μm LUV).
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Virology
Debes Hammershaimb Christiansen, Petra Elisabeth Petersen, Maria Marjunardottir Dahl, Nicolina Vest, Maria Aamelfot, Anja Brathen Kristoffersen, Mona Dverdal Jansen, Iveta Matejusova, Michael D. Gallagher, Gisli Jonsson, Eduardo Rodriguez, Johanna Hol Fosse, Knut Falk
Summary: The nonvirulent infectious salmon anaemia virus (ISAV-HPR0) causes prevalent and transient infections in all three production stages of Atlantic salmon in the Faroe Islands. Different HPR0 subgroups dominate in different stages of production, and vertical transmission of HPR0 from broodfish to progeny is uncommon.
Article
Fisheries
Kaige Song, Fei Luo, Weichao Chen, Xiaozhou Qi, Yufeng Shen, Jiwei Zha, Fei Ling, Gaoxue Wang
Summary: The ethyl acetate extract from Bacillus velezensis JW supernatant (EJW) showed highly efficient anti-SVCV activity both in vitro and in vivo, inhibiting early events of SVCV replication and increasing the survival rate of infected common carp. Furthermore, EJW up-regulated the expression of antiviral immune genes, indicating its potential as a promising antiviral strategy against SVCV.
Review
Microbiology
Lizdany Florez-Alvarez, Edmarcia Elisa de Souza, Viviane Fongaro Botosso, Danielle Bruna Leal de Oliveira, Paulo Lee Ho, Carlos Pelleschi Taborda, Giuseppe Palmisano, Margareth Lara Capurro, Joao Renato Rebello Pinho, Helena Lage Ferreira, Paola Minoprio, Eurico Arruda, Luis Carlos de Souza Ferreira, Carsten Wrenger, Edison Luiz Durigon
Summary: This article discusses the pathogenesis and treatment options for viral hemorrhagic fevers caused by hemorrhagic fever viruses (HFVs), as well as the potential for these viruses to cause global emergencies and factors favoring their spread beyond their original niches.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Zhao Zhao, Fu-Yi Jiang, Guo-Qing Zhou, Hui-Xin Duan, Jun-Yao Xia, Bin Zhu
Summary: This study developed a mannose-modified chitosan-encapsulated DNA vaccine that effectively combats SVCV, inducing a strong immune response and providing substantial protection. This could be beneficial for the development of more aquatic animal vaccines in the future.
Article
Surgery
Rafael de Athayde Soares, Marcelo Fernando Matielo, Francisco Cardoso Brochado Neto, Bruno Veloso de Melo, Ana Paula Maia Pires, Sergio Roberto Tiossi, Marcos Roberto Godoy, Roberto Sacilotto
Summary: This study found that in endovascular treatment, the angiosome concept is no longer important to limb salvage rates or ulcer/wound healing rates. Furthermore, the WIfI classification 0-1 is associated with faster and higher wound/ulcer healing rates compared to WIfI classification 2-3.
ANNALS OF VASCULAR SURGERY
(2021)
Article
Biochemistry & Molecular Biology
Daniela Cerro, Gonzalo Bustos, Carolina Villegas, Nicolas Buendia, Giannina Truffa, Maria Paz Godoy, Francisco Rodriguez, Adrian Rojas, Maria Jose Galotto, Luis Constandil, Mauricio Yanez-S, Julio Romero, Alejandra Torres
Summary: PLA/Lig-Np nanocomposites containing cinnamaldehyde (Ci) were obtained by a combination of melt extrusion and supercritical impregnation process. The developed films showed strong UV-light barrier property and good biodegradability under composting conditions. Toxicity studies in normal rats confirmed the safety of the nanocomposites for potential applications in food packaging and biomedical fields.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Microbiology
Marcos Godoy, Daniel A. Medina, Rudy Suarez, Sandro Valenzuela, Jaime Romero, Molly Kibenge, Yingwei Wang, Frederick Kibenge
Summary: This study described the molecular phylogeny of PRV based on sequence analysis of the S1 and M2 segments, strengthening the subgenotype classification and supporting research on the virulence of PRV.
Review
Fisheries
Marcos G. Godoy, Molly J. T. Kibenge, Frederick S. B. Kibenge
Summary: The outbreak of COVID-19 in workers in fish farms and processing plants has raised concerns about the safety of aquatic food products, but research suggests that the virus is unlikely to be transmitted to humans through consumption of seafood. Public health interventions such as distancing, contact tracing, and hygiene practices are key to controlling the spread of the virus in the aquaculture industry.
Article
Environmental Sciences
Jorge Mardones, Javier Paredes, Marcos Godoy, Rudy Suarez, Luis Norambuena, Valentina Vargas, Gonzalo Fuenzalida, Elias Pinilla, Osvaldo Artal, Ximena Rojas, Juan Jose Dorantes-Aranda, Kim J. Lee Chang, Donald M. Anderson, Gustaaf M. Hallegraeff
Summary: The Pseudochattonella verruculosa led to the largest farmed fish mortality ever recorded in the world, causing losses of US$ 800 M for the Chilean salmon industry in 2016. Strong climatic anomalies resulted in vertical water column stratification, promoting development of a dynamic P. verruculosa thin layer. The algal cell densities were high and contained potentially harmful fatty acids, which may have contributed to the fish mortality.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Microbiology
Rudy Suarez, Karina Kusch, Claudio D. Miranda, Tianlu Li, Javier Campanini, Phani Rama Krishna Behra, Luis Aro, Alexis Martinez, Marcos Godoy, Daniel A. Medina
Summary: The study isolated and characterized Mycobacterium strains from freshwater salmon cultures in Chile, revealing one strain related to human-infecting Mycobacterium franklinii. The isolates carried antimicrobial resistance and virulence genes, indicating their potential as etiological agents of emerging diseases in aquaculture.
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Correction
Microbiology
Rudy Suarez, Karina Kusch, Claudio D. Miranda, Tianlu Li, Javier Campanini, Phani Rama Krishna Behra, Luis Aro, Alexis Martinez, Marcos Godoy, Daniel A. Medina
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY
(2021)
Article
Rheumatology
Nathalia de Carvalho Sacilotto, Andre Ozela Augusto, Dayrana Alves Lucena, Marcos Roberto Godoy, Rogerio Duque de Almeida, Roberta de Almeida Pernambuco, Sonia Maria Alvarenga Anti Loduca Lima, Roberto Sacilotto, Rina Dalva Neubarth Giorgi
Summary: This study aimed to assess the cardiovascular risk in rheumatoid arthritis (RA) patients classified as low-risk by the Framingham score. Carotid and femoral Doppler ultrasound (DUS) were used to stratify the patients and optimize the assessment of asymptomatic cardiovascular disease. The results showed that DUS was effective in detecting cardiovascular abnormalities in RA patients, and the EULAR criteria was successful in identifying high cardiovascular risk patients.
JCR-JOURNAL OF CLINICAL RHEUMATOLOGY
(2022)
Article
Surgery
Marcus Vinicius Martins Cury, Marcelo Fernando Matielo, Rafael de Athayde Soares, Bruno Vinicius Veloso de Melo, Edson Takamitsu Nakamura, Marcos Roberto Godoy, Roberto Sacilotto
Summary: This study reports the early and long-term results of elective endovascular aneurysm repair (EVAR) in a tertiary low-volume hospital in Brazil. The study finds that while the 30-day mortality rate of EVAR in this center is high, satisfactory long-term results are still achieved.
ANNALS OF VASCULAR SURGERY
(2022)
Review
Oceanography
Jorge Mardones, Javier Paredes-Mella, Ana Flores-Lenero, Kyoko Yarimizu, Marcos Godoy, Osvaldo Artal, Andrea Corredor-Acosta, Lara Marcus, Emma Cascales, Juan Pablo Espinoza, Luis Norambuena, Rene D. Garreaud, Humberto E. Gonzalez, Jose L. Iriarte
Summary: Patagonian fjords have been experiencing severe harmful algal blooms (HABs) in recent years, particularly due to climatic anomalies and persistent thermal stratification. The bloom of Heterosigma akashiwo in Comau fjord in March-April 2021 resulted in high salmon mortalities. The outbreak was influenced by the positive phase of the Southern Annular Mode and the drought caused by low rainfall and increased water temperature.
PROGRESS IN OCEANOGRAPHY
(2023)
Article
Microbiology
Marcos Godoy, Molly J. T. Kibenge, Marco Montes de Oca, Juan Pablo Pontigo, Yoandy Coca, Diego Caro, Karina Kusch, Rudy Suarez, Ian Burbulis, Frederick S. B. Kibenge
Summary: This study investigated the pathological lesions and viral sequences of IPNV infection in genetically resistant farmed Atlantic salmon in Chile. The findings revealed an increasing number of IPN outbreaks, despite the introduction of resistant strains and vaccination strategies.
Article
Immunology
Danixa Martinez, Ricardo Oyarzun-Salazar, Ana Maria Quilapi, Jose Coronado, Ricardo Enriquez, Carolina Vargas-Lagos, Cristian Oliver, Natacha Santibanez, Marcos Godoy, Jose Luis Munoz, Luis Vargas-Chacoff, Alex Romero
Summary: This study evaluated the activation of nutritional immunity in Atlantic salmon stimulated with live and inactivated Piscirickettsia salmonis. The results showed that both live and inactivated P. salmonis could activate nutritional immunity, and there was no distinction between the two. Genetic material of P. salmonis was detected in the liver tissue of the fish. The hematocrit percentage decreased in fish stimulated with live P. salmonis, while it remained unchanged in fish stimulated with inactivated P. salmonis. Plasma iron content decreased in both experimental groups, but the decrease was statistically significant only at 3 dpi. Immune-nutritional markers were modulated in both experimental conditions, while other markers were down-regulated. Intracellular iron content increased in the liver at 7 and 14 dpi, while zinc content decreased at 14 dpi in both experimental groups. However, manganese content was not altered by the stimulation. These findings suggest that nutritional immunity responds similarly to live and inactivated P. salmonis, and may be self-activated by the detection of PAMPs rather than a sequestration and/or competition of micronutrients by the living microorganism.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Fisheries
Laura Solarte-Murillo, Alex Romero, Juan Guillermo Carcamo, Marcos Godoy, Francisco J. Morera, C. A. Loncoman
Summary: This study reveals the association of Piscine orthoreovirus (PRV) with HSMI and jaundice syndrome in coho salmon in Chile. Two variants of PRV-3 were identified, with PRV-3a being the predominant variant in recent years. Unique genetic characteristics were also found in the PRV-3 sequences from Chile. These findings emphasize the need for further research on the link between PRV and jaundice syndrome in coho salmon.
Review
Microbiology
Marcos Godoy, Marco Montes de Oca, Diego Caro, Juan Pablo Pontigo, Molly Kibenge, Frederick Kibenge
Summary: This review presents recent developments in the evolution of IAV in birds, mammals, and humans in Chile. It discusses the emergence of different IAV subtypes in poultry and pigs, the presence of reassortant AIVs incorporating genes from North and South American isolates in wild birds, and the detection of HPAI H5N1 in wild birds, sea lions, and a human along Chile's north coast. These findings highlight the need for enhanced biosecurity on poultry farms and ongoing genomic surveillance to understand and manage AIVs in both wild and domestic bird populations in Chile.
Article
Multidisciplinary Sciences
Yoandy Coca, Marcos Godoy, Juan Pablo Pontigo, Diego Caro, Vinicius Maracaja-Coutinho, Raul Arias-Carrasco, Leonardo Rodriguez-Cordova, Marco Montes de Oca, Cesar Saez-Navarrete, Ian Burbulis
Summary: An unbalanced gut microbiota in fish may promote bacterial infections. However, the interactions between different bacterial groups and their effects on fish health are not well understood. This study found that pathogenic species were present in the digestive tract and gut mucosa of healthy salmon, and the dysbiosis in sick salmon may be associated with cooperative interactions between different taxonomic families of bacteria, leading to clinical symptoms.
SCIENTIFIC REPORTS
(2023)