Article
Multidisciplinary Sciences
Nazmin Bithi, Christopher Link, Yoko O. Henderson, Suzie Kim, Jie Yang, Ling Li, Rui Wang, Belinda Willard, Christopher Hine
Summary: The study investigates the impact of dietary restriction (DR) on protein persulfidation, finding enrichment of persulfidation in liver, kidney, muscle, and brain, but decreased levels in the heart. Mice lacking cystathionine gamma-lyase (CGL) show an overall decrease in tissue protein persulfidation numbers under DR.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yafu Wang, Xiaoli Zhao, Yuehua Chen, Tony D. James, Ge Wang, Hua Zhang
Summary: Naphthalimide-based fluorescent probes with dual locked fluorescence were developed. These probes demonstrated a highly sensitive off-on fluorescence response by synergistic changes of the molecular structure and conformation in the presence of H2S and DNA. They were capable of monitoring DNA damage and evaluating apoptosis induced by H2S in living cells.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Ying Li, Yan Feng, Li Liu, Xue Li, Xin-yu Li, Xun Sun, Ke-xin Li, Rong-rong Zha, Hong-dan Wang, Meng-di Zhang, Xiong-xiong Fan, Di Wu, Yao Fan, Hao-cheng Zhang, Guo-fen Qiao, Bai-yan Li
Summary: The study showed that H2S improves the autonomic control of blood pressure regulation by increasing K-ATP channel expression in the baroreflex afferent pathway, suggesting a crucial role of H2S in cardiovascular disorders.
ACTA PHARMACOLOGICA SINICA
(2021)
Article
Biochemistry & Molecular Biology
Qiwei Hu, Rama D. Yammani, Heather Brown-Harding, David R. Soto-Pantoja, Leslie B. Poole, John C. Lukesh
Summary: Doxorubicin (DOX) is a highly effective anticancer agent, but its dose-dependent cardiotoxicity limits its long-term use. This study describes a novel H2O2-responsive DOX hybrid codrug that releases hydrogen sulfide (H2S) to maintain the antitumor effects of DOX while reducing cardiotoxic side effects.
Article
Radiology, Nuclear Medicine & Medical Imaging
Bora Nam, Woonghee Lee, Swarbhanu Sarkar, Jae-Hong Kim, Abhinav Bhise, Hyun Park, Jung Young Kim, Phuong Tu Huynh, Subramani Rajkumar, Kiwoong Lee, Yeong Su Ha, Seong Hwan Cho, Jeong Eun Lim, Kyung Won Kim, Kyo Chul Lee, Kyoungho Suk, Jeongsoo Yoo
Summary: In this study, a nuclear imaging probe was developed for in vivo detection of endogenous H2S in the brain of live mice. The probe showed excellent reactivity, sensitivity, and selectivity to H2S and accurately detected subtle changes in brain H2S concentrations. The dual modality feature of the probe allowed for characterization of increased H2S levels in neuroinflammation models at both the subcellular and whole-body scale. This probe has great potential as a novel imaging agent for diagnosing brain diseases.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2022)
Article
Agriculture, Multidisciplinary
Xijin Zhu, Xiangying Kong, Lei Zang, Nan Sun, Qunli Yu, Ling Han
Summary: This study investigated the effects of oxidative stress on early postmortem glycolysis and water-holding capacity (WHC) in yak meat. The results showed that oxidative stress promoted AMPK activation and glycolysis, reducing the WHC of yak meat. These effects were likely mediated by the CaMKK beta/AMPK signaling pathway.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Cell Biology
Malgorzata Tokarska-Schlattner, Laurence Kay, Pascale Perret, Raffaella Isola, Stephane Attia, Frederic Lamarche, Cindy Tellier, Cecile Cottet-Rousselle, Amjad Uneisi, Isabelle Hininger-Favier, Marc Foretz, Herve Dubouchaud, Catherine Ghezzi, Christian Zuppinger, Benoit Viollet, Uwe Schlattner
Summary: AMPK, a key regulator of energy homeostasis, plays important roles in maintaining cardiac function under increased workload conditions. Deletion of AMPK genes in cardiomyocytes did not affect heart function under physiological conditions, but led to alterations in response to increased workload. AMPK deletion also resulted in decreased basal metabolic rate and locomotor activity, along with changes in cardiac mitochondrial function and bioenergetics.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Swetha Pavani Rao, Wei Xie, Ye In Christopher Kwon, Nicholas Juckel, Jiashu Xie, Venkateshwara Rao Dronamraju, Robert Vince, Michael K. Lee, Swati S. More
Summary: This study demonstrates the feasibility of managing AD by enhancing 3MST function in the brain and identifies sulfanegen as a potential AD therapeutic.
Article
Environmental Sciences
Zhenjie Sun, Wenhui Gu, Zezhong Feng, Yaqin Fan, Jianfeng Niu, Guangce Wang
Summary: This study elucidated the expression regulation mechanism of the heat shock protein 70 gene in Neopyropia yezoensis under high temperature stress. The findings showed the involvement of cAMP-dependent protein kinase and activating transcription factors element in the transcription regulation of the gene. In addition, AMPK was also found to play a role in the induction of the gene. Furthermore, posttranscriptional mechanisms were proposed to be involved in the synthesis regulation of the heat shock protein 70-2 protein.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Neurosciences
Xuan Kang, Li Jiang, Fang Lan, Yi-Yun Tang, Ping Zhang, Wei Zou, Yong-Jun Chen, Xiao-Qing Tang
Summary: Hydrogen sulfide (H2S) can alleviate depression and anxiety behaviors induced by sleep deprivation, reduce hippocampal neuroinflammation, with Sirt1 playing a key role in mediating these protective effects.
BRAIN RESEARCH BULLETIN
(2021)
Review
Biochemistry & Molecular Biology
Fiona M. Russell, David Grahame Hardie
Summary: AMP-activated protein kinase (AMPK) plays a crucial role in regulating cellular energy balance by promoting ATP production and inhibiting cell growth. It may restrain aberrant growth before tumorigenesis, but support the survival of cancer cells once cancer has arisen.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Rongrong Wu, Zhongxiang Chen, Hongqi Huo, Lanlan Chen, Lichao Su, Xuan Zhang, Ying Wu, Zhicun Yao, Shenggan Xiao, Wei Du, Jibin Song
Summary: In this study, a H2S-activated ratiometric photoacoustic (PA) probe was developed for detecting the upregulation of H2S in metformin-induced liver injury. The probe showed excellent selectivity and low detection limit, and successfully imaged H2S in cells and mice.
ANALYTICAL CHEMISTRY
(2022)
Article
Cell Biology
Jee Young Sohn, Hyeok-Jin Kwak, Ji Heon Rhim, Eui-Ju Yeo
Summary: Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key glycolytic enzyme that plays a role in cellular events such as DNA repair and apoptosis. This study suggests that increased AMP-activated protein kinase (AMPK) activity may be involved in the nuclear translocation of GAPDH in senescent cells.
Article
Biochemistry & Molecular Biology
Valeria Tosello, Deborah Bongiovanni, Ludovica Di Martino, Cinzia Franchin, Paola Zanovello, Giorgio Arrigoni, Erich Piovan
Summary: This study found that AMPK signaling pathway is downregulated in T-ALL cells under nutrient-depleted conditions, leading to increased sensitivity to GLI1 expression and inhibitors. Joint targeting of AMPK and hedgehog signaling pathways significantly improved therapeutic response in T-ALL cells, suggesting a potential therapeutic strategy in rapidly expanding tumors with limited nutrient availability.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Min Chen, Wenqing Hou, Chen Chen, Yechen Wang, Yiming Xu
Summary: This work investigates the photocatalytic activity of zinc cadmium sulfide under different light wavelengths and finds that the optimal x value for the maximum hydrogen evolution rate varies. Furthermore, it is observed that the activity and stability of zinc cadmium sulfide are improved after loading ZnCo2O4.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2022)
Review
Neurosciences
Ming-ming Xu, Mao-tian Zhou, Shu-wei Li, Xue-chu Zhen, Shuang Yang
Summary: Neurodegenerative diseases such as Alzheimer's and Parkinson's are incurable and progressively debilitating conditions that mainly affect the elderly. Research has found that protein glycosylation plays a crucial role in these diseases, with abnormal changes indicating altered biological pathways in disease progression. Early detection of neurodegenerative diseases is being explored through the use of specific glycosylation changes in cerebrospinal fluid, blood, and urine as potential biomarkers.
JOURNAL OF NEUROSCIENCE RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Yu Zhang, Lei Xu, Yao Zhang, Jie Pan, Pu-qing Wang, Sheng Tian, Huan-ting Li, Bo-wen Gao, Ting-jun Hou, Xue-chu Zhen, Long-Tai Zheng
Summary: This study identified 12 compounds with better bioactivity than ISO-1 as MIF inhibitors, with compound 11 showing marked anti-inflammatory efficacy in LPS-activated BV-2 microglial cells by suppressing NF-κB and MAPKs activation. These findings suggest that small-molecule MIF inhibitors could be promising therapeutic agents for neuroinflammatory diseases.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Immunology
Long-Tai Zheng, Jiaojiao Chen, Li Zhang, Yu Zhang, Lei Xu, Tingjun Hou, Xuechu Zhen, Qijun Dai, Hua Liu
Summary: This study demonstrated that the MIF inhibitor Z-312 possesses significant anti-inflammatory effects in LPS-induced neuroinflammation by reducing proinflammatory cytokine production and modulating NF-κB and MAPK signaling pathways. Furthermore, in a PD mouse model, Z-312 markedly ameliorated microglial activation and subsequent DA neuron loss. The findings suggest that Z-312 may be a promising neuroprotective agent for the treatment of neuroinflammation-mediated neurological diseases.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mengmeng Jin, Katja Akguen, Tjalf Ziemssen, Markus Kipp, Rene Guenther, Andreas Hermann
Summary: The study revealed that Th17 cells and IL-17A are directly involved in the degeneration of motor neurons in ALS patients. Th17 cells from MS patients induced severe MN degeneration, while IL-17A led to a dose-dependent decline in MN viability and neurite length. Neutralizing IL-17A and anti-IL-17 receptor A treatment reversed all effects of IL-17A, highlighting the importance of this pathway in ALS pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Junjie Cheng, Rong Zhang, Zhirou Xu, Youliang Ke, Renjuan Sun, Huicui Yang, Xiaohu Zhang, Xuechu Zhen, Long-Tai Zheng
Summary: The study suggests that glycolysis plays a crucial role in microglial activation and inhibiting glycolysis can attenuate neuroinflammatory diseases associated with microglial activation.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Neurosciences
Hai-Yue Tu, Yong-Quan Gu, Xia Li, Shao-Fang Pei, Li -Fang Hu, Ya-Li Wang
Summary: Parkinson's disease (PD) is the second most common neurodegenerative disorder, affecting dopaminergic neurons. Abnormal autophagy and circadian dysfunction are two characteristics of PD. Blood sample analysis revealed decreased levels of BECN1 and LAMP2 in PD patients, suggesting a potential manifestation of abnormal biological rhythm in PD.
NEUROSCIENCE LETTERS
(2021)
Article
Cell & Tissue Engineering
Ranjie Xu, Andrew J. Boreland, Xiaoxi Li, Caroline Erickson, Mengmeng Jin, Colm Atkins, Zhiping P. Pang, Brian P. Daniels, Peng Jiang
Summary: By coculturing human pluripotent stem cell (hPSC)-derived microglia and primitive macrophage progenitors, a new brain region-specific organoid model containing microglia has been established. This model allows control of the number of human microglia, which exhibit phagocytic activity and synaptic pruning function. Additionally, human microglia in the organoids respond to Zika virus infection, providing a novel platform to study human microglial function in various neurological disorders.
Article
Chemistry, Multidisciplinary
Peng-ju Yan, Zhao-xiang Ren, Zhi-feng Shi, Chun-lei Wan, Chao-jun Han, Liu-shuai Zhu, Ning-ning Li, John L. Waddington, Xue-chu Zhen
Summary: This study reveals that deficiency of Clk1 suppresses METH-induced conditioned place preference (CPP) and increases DAT expression on the cell membrane of striatum and hippocampus. The decrease in iron content in the brain regions is due to elevated expression of iron exporter ferroportin 1 (FPN1) associated with increased expression of hypoxia-inducible factor-1 alpha (HIF-1 alpha) in response to Clk1 deficiency. Iron plays a critical role in mediating Clk1 deficiency-induced alterations in DAT expression via upstream HIF-1 alpha.
ACTA PHARMACOLOGICA SINICA
(2022)
Article
Cell Biology
Hai-Yue Tu, Bao-Shi Yuan, Xiao-Ou Hou, Xiao-Jun Zhang, Chong-Shuang Pei, Ya-Ting Ma, Ya-Ping Yang, Yi Fan, Zheng-Hong Qin, Chun-Feng Liu, Li-Fang Hu
Summary: The extracellular transfer of alpha-Synuclein (alpha-Syn) contributes to Parkinson's disease pathogenesis by activating microglia and neuroinflammation. This study found that extracellular alpha-Syn inhibits microglia autophagy through Toll-like receptor 4 (Tlr4)-mediated p38 and Akt-mTOR signaling pathways, leading to neuroinflammation and PD development.
Article
Biochemistry & Molecular Biology
Xiao-Ou Hou, Hai-Yue Tu, Hai-Chun Qian, Qian Li, Ya-Ping Yang, Guo-Qiang Xu, Fen Wang, Chun-Feng Liu, Ya-Li Wang, Li-Fang Hu
Summary: This study found that exogenous H2S donors can enhance autophagic flux through the AMPK-mTOR signaling pathway, thus reducing the accumulation of alpha-synuclein in dopaminergic cells.
NEUROCHEMISTRY INTERNATIONAL
(2021)
Article
Neurosciences
Tao He, Chaojun Han, Chun Liu, Jiaojiao Chen, Huicui Yang, Longtai Zheng, John L. Waddington, Xuechu Zhen
Summary: This study found that methamphetamine abuse can lead to damage of dopaminergic neurons, and revealed the important role of D1 receptors in this damage. The study also found that the AMPK/FOXO3A pathway mediates the activation of autophagy mediated by D1 receptors.
PSYCHOPHARMACOLOGY
(2022)
Article
Chemistry, Multidisciplinary
An-Qi Dong, Ya-Ping Yang, Shu-Min Jiang, Xiao-Yu Yao, Di Qi, Cheng-Jie Mao, Xiao-Yu Cheng, Fen Wang, Li-Fang Hu, Chun-Feng Liu
Summary: This study demonstrates the anti-neuroinflammatory activity of PPX through enhancing autophagy activity in astrocytes, which provides a new mechanism for the beneficial effect of PPX in Parkinson's disease therapy.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Cell & Tissue Engineering
Mengmeng Jin, Ranjie Xu, Le Wang, Mahabub Maraj Alam, Ziyuan Ma, Sining Zhu, Alessandra C. Martini, Azadeh Jadali, Matteo Bernabucci, Ping Xie, Kelvin Y. Kwan, Zhiping P. Pang, Elizabeth Head, Ying Liu, Ronald P. Hart, Peng Jiang
Summary: Using iPSC-based organoid and chimeric mouse models, researchers found that microglia in Down syndrome exhibit enhanced synaptic pruning function and elevated type-I interferon signaling, which can be rescued by knocking down the gene encoding type I interferon receptors. This study provides in vivo evidence of human microglial response to pathological tau and suggests that targeting interferon receptors may improve microglial functions.
Article
Cell & Tissue Engineering
Mengmeng Jin, Mahabub Maraj Alam, Alice Y. -C. Liu, Peng Jiang
Summary: Immunodeficient mice are widely used in human stem cell transplantation research. This study found that in Rag2-/- mouse brains, lipofuscin accumulation occurs earlier and spreads more extensively, emitting strong autofluorescence. Surprisingly, in Rag1-/- mouse brains, lipofuscin autofluorescence appears at much older ages compared to Rag2-/- mice. These findings provide important background information for studies using aged immunodeficient mice.
Review
Pharmacology & Pharmacy
Zheng Ying, Na Ye, Qilian Ma, Fan Chen, Ningning Li, Xuechu Zhen
Summary: Significant evidences show that sub-cellular organelle dynamics play a critical role in physiological and pathological events, making them attractive drug targets. However, drug development in this field is still limited despite extensive studies on the biological mechanisms. This review explores the potential of targeting organelle dynamics in the central nervous system, specifically mitochondrial dynamics and ER-organelle membrane contact dynamics, for treating neurodegenerative diseases.
ADVANCED DRUG DELIVERY REVIEWS
(2023)