Article
Biochemistry & Molecular Biology
Jiandong Wu, Anna Chernatynskaya, Annalise Pfaff, Huari Kou, Nan Cen, Nuran Ercal, Honglan Shi
Summary: Oxidative stress may contribute to various diseases, and understanding the changes in endogenous thiols, particularly glutathione (GSH) and its metabolites, in response to oxidative insult is crucial for prevention and treatment. A new method using high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS) was developed to simultaneously determine seven sulfur-containing metabolites, providing insights into thiol metabolism and oxidative stress responses.
Article
Biochemistry & Molecular Biology
Syed Masood, Edward R. Pennington, Steven O. Simmons, Philip A. Bromberg, Saame R. Shaikh, Rebecca L. Rice, Avram Gold, Zhenfa Zhang, James M. Samet
Summary: Exposure to respirable air particulate matter (PM2.5) can lead to health issues, and one major source of PM2.5 is the photooxidation of volatile plant-produced organic compounds like isoprene. This process generates oxidants that cause inflammatory and adaptive gene expression in human airway epithelial cells (HAEC). Through experiments, it was found that isoprene hydroxyhydroperoxides (ISOPOOH) can oxidize glutathione in HAEC, both directly and indirectly through lipid hydroperoxides. These findings highlight the potential contribution of ISOPOOH to the oxidative burden in human airways caused by inhalation of secondary organic aerosols.
Article
Cell Biology
Neeraj Dholia, Gurupreet S. Sethi, Amarjit S. Naura, Umesh C. S. Yadav
Summary: The study found that LTD4 can induce inflammatory response in human airway epithelial cells by activating the NALP3 inflammasome, and further promote airway epithelial cells' remodeling through the TGF-beta/smad2/3-mediated pathway. Additionally, obese mice challenged with OVA showed asthma-like lung inflammation and remodeling phenotypes.
INFLAMMATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Marie Pacoud, Karine Mandon, Julie Cazareth, Olivier Pierre, Pierre Frendo, Genevieve Alloing
Summary: Reactive oxygen species, such as hydrogen peroxide, play a crucial role in controlling the establishment and functioning of Rhizobium-legume symbiosis. This study used biosensors to measure the intracellular redox state of Sinorhizobium meliloti, a bacterium involved in nitrogen-fixing symbiosis. The results revealed an oxidative shift in the redox status of S. meliloti during bacteroid differentiation. These findings provide new opportunities for studying redox dynamics during N2-fixing symbiosis.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Chemistry, Analytical
Xinmiao Lu, Muyu Wu, Siwen Wang, Jingcan Qin, Peiyong Li
Summary: In this study, a near infrared fluorescent probe (HI) was developed to detect and report intracellular cysteine and glutathione, and monitor the development of drug resistance in tumor. The probe showed strong near infrared fluorescence in response to cysteine and glutathione, with high specificity and no interference from other amino acids. Incubation with PATU-8988 and PATU-8988/Fu cells allowed for clear imaging and monitoring of intracellular cysteine and glutathione, while application to tumor-bearing mice facilitated monitoring and reporting of drug resistance.
Article
Medicine, Research & Experimental
Zuo Yang, Chaoqiang Qiao, Qian Jia, Zhuang Chen, Xiaofei Wang, Xuelan Liu, Ruili Zhang, Kanyi Pu, Zhongliang Wang
Summary: In this study, a versatile nano-vehicle PBMLR was developed to comprehensively and sustainably modulate the intracellular environment for enhancing the efficacy of photodynamic therapy. The metabolic pre-intervention strategy effectively circumvented the limitations of intracellular metabolic avenues, leading to the enhanced generation of singlet oxygen radicals and improved therapeutic outcomes.
Review
Biochemistry & Molecular Biology
Alessandra Fraternale, Carolina Zara, Marta De Angelis, Lucia Nencioni, Anna Teresa Palamara, Michele Retini, Tomas Di Mambro, Mauro Magnani, Rita Crinelli
Summary: Host-directed therapy using drugs targeting cellular pathways required for virus lifecycle or its clearance could be an effective approach for treating infectious diseases. Redox homeostasis, especially intracellular glutathione (GSH) depletion, plays a crucial role in virus replication and pathogenesis of virus-induced diseases, as well as in maintaining a balance between Th1/Th2 immune responses. Overproduction of reactive oxygen species (ROS) by viral infection can activate nuclear factor (NF)-kB, which regulates viral and host gene expression related to viral replication and inflammatory responses.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Chenlu Wang, Hongxin Lin, Xiaoguang Ge, Jing Mu, Lichao Su, Xuan Zhang, Meng Niu, Huanghao Yang, Jibin Song
Summary: A NIR-IIb nanoprobe was developed for in vivo ratiometric imaging of GSH with high detection limit and linear relationship. The nanoprobe successfully detected and imaged GSH in both subcutaneous tumor and orthotopic colon tumor with high accuracy and resolution.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Food Science & Technology
Jestin Thomas, Anand Patel, Syam Das Sivadasan, Sheethal Sreevallabhan, Krishnakumar Illathu Madhavamenon, Ratheesh Mohanan
Summary: This study investigated the effects of clove buds polyphenol extract (Clovinol) and synthetic glutathione (s-GSH) on metabolic syndrome. The results showed that Clovinol significantly enhanced antioxidant capacity, improved blood sugar and lipid metabolism, and had no significant side effects.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Biophysics
Jinho Yoon, Minkyu Shin, Dongyeon Kim, Joungpyo Lim, Hyun-Woong Kim, Taewook Kang, Jeong-Woo Choi
Summary: The study developed a novel bionanohybrid structure to control the intracellular redox states of living cells using the capacitance of MoS2, enabling the construction of a cell-based biomemory device. This innovation offers a new approach to manipulate cellular redox states in cell-based bioelectronic applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Biochemistry & Molecular Biology
Jan Stefaniak, Barbara Lata
Summary: The study assessed the enzymatic and non-enzymatic antioxidant status of kiwiberry leaves under different N regimes in two cultivars, showing significant fluctuations in enzymatic antioxidant activity and slight increases in total phenolic content. Two peaks of TEAC increase were observed at the start and end of the growing season, with a negative correlation between leaf N content and ABTS and FRAP tests.
Article
Environmental Sciences
Pinar Arslan
Summary: This study evaluated the effects of cyphenothrin on freshwater mussels and found that antioxidant enzyme glutathione and total hemocyte count could serve as biomarkers to assess the response of freshwater mussels to pollutants.
WATER AIR AND SOIL POLLUTION
(2022)
Article
Plant Sciences
Kai Ni, Jia Guo, Bing Bu, Yan Pan, Jingjing Li, Lei Liu, Mingzhi Luo, Linhong Deng
Summary: This study evaluated the effects of a group of bitter flavonoids derived from fruits on the proliferation of human airway epithelial-like cells cultured in vitro. The results showed that five out of the six tested bitter tastants inhibited the proliferation of AECs, while only naringin promoted proliferation.
Article
Veterinary Sciences
Yang Liu, Zhaoju Deng, Siyu Xu, Gang Liu, Yushan Lin, Sohrab Khan, Jian Gao, Weijie Qu, John P. Kastelic, Bo Han
Summary: Mycoplasma infection induces autophagy in bMEC, but subsequently impairs the autophagy flux by inhibiting autophagosome maturation and lysosomal function.
VETERINARY RESEARCH
(2021)
Review
Substance Abuse
Huihui Lin, Hequan Li
Summary: Asthma is a common chronic airway inflammatory disease that can lead to irreversible or partially irreversible airflow limitation and persistent airway hyperresponsiveness in patients. This review focuses on the effects of smoking on airway remodeling in asthmatics and the progress in evaluating airway remodeling.
TOBACCO INDUCED DISEASES
(2023)
