Article
Microbiology
E. D. Pieterman, S. van den Berg, A. van der Meijden, E. M. Svensson, H. Bax, J. E. M. de Steenwinkel
Summary: Increasing the dosage of rifampin and rifapentine in HFIM did not always lead to improved antimycobacterial activity, and drug resistance was found to be unrelated to drug exposure for both drugs. Further parameters and readouts may be needed for proper assessment of the translational value of HFIM in preclinical models.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Elisa H. Ignatius, Mahmoud Tareq Abdelwahab, Bronwyn Hendricks, Nikhil Gupte, Kim Narunsky, Lubbe Wiesner, Grace Barnes, Rodney Dawson, Kelly E. Dooley, Paolo Denti
Summary: Shorter, more potent regimens are needed for tuberculosis, and pretomanid offers potential as a treatment-shortening agent. However, it remains unclear how pretomanid interacts with key first-line drugs like rifampin and whether concurrent use may impact its efficacy.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Pharmacology & Pharmacy
Gunavanthi D. Boorgula, Laxmi U. M. R. Jakkula, Tawanda Gumbo, Bockgie Jung, Shashikant Srivastava
Summary: The study compared microbial kill and resistance emergence of different rifamycins in the treatment of pulmonary MAC infection. Results showed no significant difference in efficacy and resistance suppression among the three rifamycins tested, and monotherapy with rifamycins was ineffective and led to drug resistance development.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Infectious Diseases
Ma-chao Li, Jie Lu, Yao Lu, Tong-yang Xiao, Hai-can Liu, Shi-qiang Lin, Da Xu, Gui-lian Li, Xiu-qin Zhao, Zhi-guang Liu, Li-li Zhao, Kang-lin Wan
Summary: The study identified 34 mutations within the rpoB gene, with 25 of them altering structural interactions with RIF and contributing to resistance. Specific mutations such as S450L, H445D, H445Y, H445R were associated with high-level resistance, while D435V was linked to moderate resistance.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Multidisciplinary Sciences
Manse Kim, Claire E. Johnson, Alan A. Schmalstig, Ayano Annis, Sarah E. Wessel, Brian Van Horn, Amanda Schauer, Agata A. Exner, Jason E. Stout, Angela Wahl, Miriam Braunstein, J. Victor Garcia, Martina Kovarova
Summary: This study develops a long-acting delivery system for the anti-tuberculosis drug rifabutin, which is made of biodegradable polymers and biocompatible solvents. The formulation shows high drug load and sustained drug release, and demonstrates translational potential in animal models.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Frank Kloprogge, Julio Ortiz Canseco, Lynette Phee, Zahra Sadouki, Karin Kipper, Adam A. Witney, Neil Stoker, Timothy D. McHugh
Summary: This study investigated the concentration dependency of isoniazid-rifampicin resistance emergence and found that rifampicin resistance is the result of non-specific mechanisms, while part of isoniazid resistance is associated with the accumulation of specific SNPs. Additionally, the study showed that resistance does not emerge in concentration-time profiles mimicking the granuloma.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Zahra Hosseinali, Jafar Mohammadshahi, Amir Teimourpour, Shahram Habibzadeh, Majid Esmaelizad, Mohsen Arzanlou, Hafez Mirzanejad-Asl, Mahin Sadeghnezhad, Shabnam Sohrabi, Roghayeh Teimourpour
Summary: This study aimed to molecularly detect resistance to first-line drugs against Mycobacterium tuberculosis. The results revealed a higher resistance rate to isoniazid and rifampin among non-tuberculous mycobacteria (NTM) compared to Mycobacterium tuberculosis complex (MTC) strains.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Microbiology
Yingda L. Xie, Nisha Modi, Deborah Handler, Sijia Yu, Prakruti Rao, Leonid Kagan, Kristen Petros de Guex, Robert Reiss, Anna Siemiatkowska, Anshika Narang, Navaneeth Narayanan, Jasie Hearn, Amanda Khalil, Patricia Woods, Laura Young, Alfred Lardizabal, Selvakumar Subbian, Charles A. Peloquin, Christopher Vinnard, Tania A. Thomas, Scott K. Heysell
Summary: Urine spectrophotometry can accurately predict serum rifampin underexposure in adult TB patients, providing critical information for optimizing tuberculosis treatment outcomes. This simple and cost-effective test is of great importance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Ming-Chih Yu, Ching-Sheng Hung, Chun-Kai Huang, Cheng-Hui Wang, Yu-Chih Liang, Jung-Chun Lin
Summary: Drug resistance in Mycobacterium tuberculosis is a global health issue, and traditional methods for detecting resistance are time-consuming and inefficient. In this study, a whole-genome sequencing pipeline using the Nanopore MinION platform was developed to diagnose drug-resistant MTB isolates more accurately and quickly.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Ma-Chao Li, Xiao-Yue Wang, Tong-Yang Xiao, Shi-Qiang Lin, Hai-Can Liu, Cheng Qian, Da Xu, Gui-Lian Li, Xiu-Qin Zhao, Zhi-Guang Liu, Li -Li Zhao, Kang-Lin Wan
Summary: This study assessed the relationship between variant rpoB mutations and the degree of rifampin/rifabutin resistance in Mycobacterium tuberculosis. The results revealed that certain mutations were associated with resistance to both drugs, while others were only associated with rifampin resistance. Interestingly, some isolates showed resistance to rifampin but susceptibility to rifabutin.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Immunology
Jonathon R. Campbell, Hamdan Al-Jahdali, Boubacar Bah, Marcia Belo, Victoria J. Cook, Richard Long, Kevin Schwartzman, Anete Trajman, Dick Menzies
Summary: The study suggests that rifampin is as safe and effective as isoniazid in populations with various health conditions including HIV.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Marije Van Schalkwyk, Adrie Bekker, Eric Decloedt, Jiajia Wang, Gerhard B. Theron, Mark F. Cotton, Ahizechukwu C. Eke, Tim R. Cressey, David E. Shapiro, Kira Bacon, Kevin Knowles, Kathleen George, Renee Browning, Nahida Chakhtoura, Kittipong Rungruengthanakit, Lubbe Wiesner, Edmund V. Capparelli, Alice M. Stek, Mark Mirochnick, Brookie M. Best
Summary: Physiological changes during pregnancy can affect the pharmacokinetics of antituberculosis drugs. This study investigates the impact of pregnancy on the pharmacokinetics of rifampin, isoniazid, ethambutol, and pyrazinamide in individuals without HIV. The results show that isoniazid and ethambutol concentrations were lower during pregnancy compared to postpartum concentrations, while rifampin and pyrazinamide concentrations were similar.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Pierre Santucci, Beren Aylan, Laure Botella, Elliott M. Bernard, Claudio Bussi, Enrica Pellegrino, Natalia Athanasiadi, Maximiliano G. Gutierrez
Summary: This study investigates the effect of intracellular environments on the efficacy of antibiotics against Mycobacterium tuberculosis. By using a dual live imaging approach, the researchers found that intracellular acidification is a prerequisite for the antibacterial efficacy of pyrazinamide, a first-line anti-TB drug.
Article
Infectious Diseases
Elisa Lopez-Varela, Ahmed A. Abulfathi, Natasha Strydom, Pierre Goussard, Abraham C. van Wyk, Anne Marie Demers, Anneen Van Deventer, Anthony J. Garcia-Prats, Johannes van der Merwe, Matthew Zimmerman, Claire L. Carter, Jacques Janson, Julie Morrison, Helmuth Reuter, Eric H. Decloedt, James A. Seddon, Elin M. Svensson, Rob Warren, Radojka M. Savic, Veronique Dartois, Anneke C. Hesseling
Summary: This study aimed to determine the concentrations of first-line antituberculosis drugs at the site of disease in children with intrathoracic TB. The results showed that, except for isoniazid, other drugs had low exposure at the site of disease in current TB treatment for children.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Medicine, Research & Experimental
Mei-Chun Zeng, Qing-Jun Jia, Lei-Ming Tang
Summary: The study aimed to analyze genetic mutations in rpoB gene of rifampin-resistant Mycobacterium tuberculosis isolates from Zhejiang, China. The most frequent mutation was S450L, and mutations associated with poly-drug resistant, pre-XDR, and XDR TB were mainly located at codon 445 or 450 in the RRDR. Further studies are needed to clarify the relationships between RIF(R) and specific mutations.
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2021)
Article
Public, Environmental & Occupational Health
Lika Apriani, Susan McAllister, Katrina Sharples, Isni Nurul Aini, Hanifah Nurhasanah, Dwi Febni Ratnaningsih, Agnes Rengga Indrati, Rovina Ruslami, Bachti Alisjahbana, Reinout van Crevel, Philip C. Hill
Summary: Medical and nursing students entering clinical training in high TB-endemic areas are at high risk of M. tuberculosis infection. Improved infection prevention and control strategies are needed to protect these students.
TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE
(2022)
Letter
Immunology
Willyhelmina Olomi, Irene Andia Biraro, Kajiru Kilonzo, Lindsey te Brake, Davis Kibirige, Nyasatu Chamba, Nyanda Elias Ntinginya, Issa Sabi, Julia Critchley, Katrina Sharples, Philip C. Hill, Reinout Van Crevel
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Pharmacology & Pharmacy
Carlijn H. C. Litjens, Laurens F. M. Verscheijden, Celine Bolwerk, Rick Greupink, Jan B. Koenderink, Petra H. H. van den Broek, Jeroen J. M. W. van den Heuvel, Elin M. Svensson, Martin J. Boeree, Cecile Magis-Escurra, Wouter Hoefsloot, Reinout van Crevel, Arjan van Laarhoven, Jakko van Ingen, Saskia Kuipers, Rovina Ruslami, David M. Burger, Frans G. M. Russel, Rob E. Aarnoutse, Lindsey H. M. Te Brake
Summary: The study investigated the pharmacokinetic interaction of moxifloxacin with rifampicin in tuberculosis treatment, providing new insights and potential therapeutic strategies. Simulations demonstrated that increasing the dose of moxifloxacin could restore plasma exposure in patients.
JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Article
Immunology
Cas J. Isfordink, Colette Smit, Anders Boyd, Marieke J. A. de Regt, Bart J. A. Rijnders, Reinout van Crevel, Robin P. Ackens, Peter Reiss, Joop E. Arends, Marc van der Valk
Summary: Low prevalence of HCV-viremic people with HIV in the Netherlands coincides with widespread DAA uptake. Barriers to DAA uptake are mostly patient-related, and the risk of HCV transmission from untreated individuals in care is low.
Article
Immunology
Mihai G. Netea, Jorge Dominguez-Andres, Frank L. van de Veerdonk, Reinout van Crevel, Bali Pulendran, Jos W. M. van der Meer
Summary: Individuals exposed to SARS-CoV-2 may exhibit natural resistance to infection, with variability in susceptibility observed. Factors contributing to natural resistance include host genetic variants, viral interference, and innate immune responses. Understanding natural resistance can assist in developing better preventive and therapeutic measures for future pandemics.
TRENDS IN IMMUNOLOGY
(2022)
Review
Public, Environmental & Occupational Health
Sofiati Dian, Ahmad Rizal Ganiem, Savira Ekawardhani
Summary: Toxoplasma gondii infection in the central nervous system is common among immunodeficient patients, particularly in countries with a high burden of HIV and low coverage of antiretroviral drugs. The brain is a favored site for infection due to its low inflammatory reaction. The diagnosis of cerebral toxoplasmosis is mainly based on clinical symptoms, serology examination, and neuroimaging, although new diagnostic tools are being explored. Clinical trials are needed to develop new therapies and vaccines for this infection.
PATHOGENS AND GLOBAL HEALTH
(2023)
Article
Biology
Edwin Ardiansyah, Julian Avila-Pacheco, Le Thanh Hoang Nhat, Sofiati Dian, Dao Nguyen Vinh, Hoang Thanh Hai, Kevin Bullock, Bachti Alisjahbana, Mihai G. Netea, Riwanti Estiasari, Trinh Thi Bich Tram, Joseph Donovan, Dorothee Heemskerk, Tran Thi Hong Chau, Nguyen Duc Bang, Ahmad Rizal Ganiem, Rovina Ruslami, Valerie A. C. M. Koeken, Raph L. Hamers, Darma Imran, Kartika Maharani, Vinod Kumar, Clary B. Clish, Reinout van Crevel, Guy Thwaites, Arjan van Laarhoven, Nguyen Thuy Thuong Thuong
Summary: Metabolomic analysis of tuberculous meningitis patients revealed that levels of tryptophan in cerebrospinal fluid were negatively associated with survival. Additionally, downstream metabolites of tryptophan were correlated with cerebrospinal fluid inflammation and blood-cerebrospinal fluid leakage, and plasma kynurenine levels predicted mortality.
Article
Hematology
Eswi Zenobia, Nushrotul Lailiyya, Sofiati Dian, Cep Juli, Asep Nugraha Hermawan, Lisda Amalia
Summary: This study aimed to describe the levels of P-selectin in patients with Cerebral Venous Sinus Thrombosis (CVST). The results showed that P-selectin levels were associated with subacute onset, infectious etiology, duration of treatment <3 Months, past history of hyper aggregation, hypercoagulable state, increased D-dimer, normal fibrinogen, and multiple affected sinuses. P-selectin could potentially serve as a diagnostic marker for hyper aggregation and hypercoagulable state in CVST patients, but further research is needed to confirm this.
JOURNAL OF BLOOD MEDICINE
(2023)
Article
Rheumatology
Lara D. Veeken, Merel A. A. Opdam, Lise M. Verhoef, Calin Popa, Reinout van Crevel, Alfons A. den Broeder
Summary: This study aims to determine the infection incidence in a large real-life population of RA patients using RTX, with special focus on (ultra-)low dosing and time since last infusion. The results showed that the risk of infection was lower with a dose of 200 mg, and infections seemed to occur more frequently in the first two months after infusion, suggesting an association with peak concentration.
Article
Microbiology
Ralf Stemkens, Maarten Cobussen, Wouter Hoefsloot, Erik de Laat, Reinout van Crevel, Rob E. Aarnoutse, Jakko van Ingen
Summary: Treatment of skin and soft tissue infections caused by nontuberculous mycobacteria can be challenging. Negative pressure wound therapy with instillation and dwell time of topical antibiotics can be a successful and well-tolerated treatment option.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Review
Clinical Neurology
Sofiati Dian, Ahmad Rizal Ganiem, Lindsey H. M. te Brake, Arjan van Laarhoven
Summary: Neurotuberculosis is the most severe form of tuberculosis with a high mortality rate. Early treatment is crucial, but diagnosing and treating neurotuberculosis remains challenging. New antibiotic regimens and host-directed therapies are being studied to combat drug resistance and improve patient outcomes.
Article
Medicine, General & Internal
Caryn M. Upton, Rob C. van Wijk, Laurynas Mockeliunas, Ulrika S. H. Simonsson, Kirsten McHarry, Gerben van den Hoogen, Chantal Muller, Arne von Delft, Helene-Mari van der Westhuizen, Reinout van Crevel, Gerhard Walzl, Pedro M. Baptista, Jonathan Peter, Andreas H. Diacon
Summary: This study evaluated the impact of BCG vaccination on the incidence and mortality of COVID-19 in healthcare workers in South Africa, and the results showed that BCG had no significant effect in preventing COVID-19.
Article
Medicine, Research & Experimental
Bowen Zhang, Simone J. C. F. M. Moorlag, Jorge Dominguez-Andres, Ozlem Bulut, Gizem Kilic, Zhaoli Liu, Reinout van Crevel, Cheng-Jian Xu, Leo A. B. Joosten, Mihai G. Netea, Yang Li
Summary: Trained immunity refers to the long-lasting memory traits of innate immunity. This study investigates the cellular transcriptional programs in monocyte populations upon the induction of trained immunity, identifying three monocyte subpopulations. The findings are also consistent with gene signatures in patients with immune-mediated diseases.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Review
Medicine, Research & Experimental
Todia P. Setiabudiawan, Remi K. Reurink, Philip C. Hill, Mihai G. Netea, Reinout van Crevel, Valerie A. C. M. Koeken
Summary: BCG, developed 100 years ago, is the only licensed tuberculosis vaccine and is widely used worldwide. However, despite universal vaccination policies, TB burden remains high. The level of protection provided by BCG varies between age groups and settings. This review presents the evidence and factors influencing BCG's ability to protect against TB, as well as the immunological mechanisms involved. Possible strategies to boost BCG's efficacy are also discussed.
Article
Public, Environmental & Occupational Health
Annisa Rahmalia, Rudi Wisaksana, Marie Laga, Reinout van Crevel, Koen Peeters Grietens
Summary: This study examined the disclosure of HIV status and partner testing among women living with HIV in Bandung, Indonesia. The findings showed that women faced barriers in disclosing their status and getting their partners tested. Women with longstanding HIV infections in particular would benefit from partnership counseling and specific guidelines for partner notification and testing.
SEXUAL AND REPRODUCTIVE HEALTH MATTERS
(2022)