Article
Medicine, General & Internal
Ji-Na Gu, Lin Chen, Xing-Bei Weng, Xiao-Yan Yang, Dan-Mei Pan
Summary: The research revealed that Escherichia coli Huamei202001 belongs to sequence type 410 and carries a blaNDM-5-encoding IncX3-type plasmid responsible for carbapenem resistance spreading. Only the patient was detected as having a positive faecal sample screening test for CRE.
FRONTIERS IN MEDICINE
(2021)
Article
Microbiology
Tsukasa Ariyoshi, Kotaro Aoki, Hiroaki Kubota, Kenji Sadamasu, Yoshikazu Ishii, Kazuhiro Tateda
Summary: This study characterized a bla(NDM-16b)-carrying IncX3 plasmid in an Escherichia coli strain isolated from a hospital inpatient in Tokyo, Japan. The plasmid differed in sequence from previously reported bla(NDM) variants and showed high resistance to beta-lactam antimicrobials except for aztreonam.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Hui Zuo, Yo Sugawara, Shizuo Kayama, Sayoko Kawakami, Koji Yahara, Motoyuki Sugai
Summary: The spread of the gene encoding NDM-type carbapenemase poses a serious threat to clinical practice and public health. This study analyzed IncX3-type plasmids carrying bla (NDM-5) and bla (NDM-16b) in Japan, revealing their high similarity and widespread dissemination in Asian countries.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Zhenbao Ma, Zhenling Zeng, Jiao Liu, Chang Liu, Yu Pan, Yanan Zhang, Yafei Li
Summary: This study investigated the prevalence of bla(NDM-5) in pigs and analyzed the molecular characteristics of NDM-5-producing Escherichia coli isolates in Guangdong province, China. The results revealed that bla(NDM-5) was found in IncX3 and IncHI2 plasmids, with IncHI2 plasmids containing a typical IncHI2 plasmid backbone region and a multiresistance region (MRR). Continued surveillance for the dissemination of bla(NDM-5) among food-producing animals is necessary to understand and control carbapenem resistance.
Article
Microbiology
Baomo Liu, Yingyi Guo, Ningjing Liu, Jiong Wang, Feifeng Li, Likang Yao, Chao Zhuo
Summary: IncX3 plasmids have been prevalent in China over the last 10 years, and are specifically associated with hospital-isolated Escherichia coli ST410. Despite the highly conservative backbone, the resistance genetic contexts on these plasmids could fall into different subtypes, indicating the spread of resistance gene environment across different bacterial genera. It is crucial to adopt stringent infection control measures to target IncX3 plasmid spread.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Yanyun Gao, Pengcheng Du, Pei Zhang, Juan Wang, Ziyi Liu, Seamus Fanning, Zhiqiang Wang, Ruichao Li, Li Bai
Summary: The evolutionary patterns of blaNDM-1-carrying fusion plasmids are largely unknown. A study reported a blaNDM-1-bearing fusion plasmid pZX35-269k, which exhibited structural instability and formed various daughter plasmids during conjugation and passage. The study confirmed the transmission and structural evolution of the plasmid in both laboratory and clinical settings, highlighting the potential risk for public health.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Infectious Diseases
Mingju Hao, Jessica Schuyler, Haifang Zhang, Elena Shashkina, Hong Du, Derrick E. Fouts, Michael Satlin, Barry N. Kreiswirth, Liang Chen
Summary: A study on CRKp isolates from New York and New Jersey revealed low in vitro activity of apramycin and a high resistance rate, mainly among the K. pneumoniae ST258 strains. The study identified the presence of a novel cointegrate plasmid, p59494-RX116.1, demonstrating the potential spread of apramycin resistance through plasmids.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Xiaofeng Hu, Lang Yang, Nian Dong, Yanfeng Lin, Ling Zhang, Xu Wang, Xudong Guo, Ying Xiang, Lianqun Jin, Chuanfu Zhang, Yansong Sun, Shaofu Qiu, Peng Li, Hongbin Song
Summary: This study investigated the dissemination of the bla(NDM-5) gene in carbapenem-resistant Escherichia coli isolates from different regions in China. Seven bla(NDM-5)-positive E. coli strains were detected, which showed resistance to multiple antibiotics and carried other antimicrobial resistance genes. The bla(NDM-5) gene was located on 46 kb IncX3 plasmids in all isolates. This study confirms the widespread dissemination of bla(NDM-5)-carrying plasmids in E. coli isolates in China, highlighting the need for stringent surveillance and control measures.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Public, Environmental & Occupational Health
Xiaofan Zhang, Lingxia Chen, Xia Zhang, Qian Wang, Jingjing Quan, Jintao He, Hongying Pan, Xi Li
Summary: This study identified the co-existence of an IncHI2 plasmid carrying mcr-1.1 and an IncI1-I plasmid carrying blaNDM-5 in a clinical E. coli isolate for the first time. The research expands the known diversity of plasmids in NDM-5-producing Enterobacterales strains.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2022)
Article
Infectious Diseases
Junwei Huang, Jianjun Zhu, Daojun Gong, Lin Wu, Yongze Zhu, Liqing Hu
Summary: This study reported a clinical E. coli ST167 strain carrying a novel IncFII-type bla(ND)(M-5) plasmid obtained from abdominal infection in China. The presented genome sequences of the bla(NDM-)(5)-producing E. coli strain ST167 could provide further insight into the acquisition of multiple resistance genes by this successful lineage.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Infectious Diseases
Lin Sun, Guiling Li, Nan Meng, Zhenyu Wang, Hanyun Wang, Jing Wang, Xinan Jiao
Summary: A carbapenem-resistant Klebsiella quasipneumoniae isolate harboring the blaNDM-7 gene was recovered from a patient in Jiangsu Province, China. This is the first identification of an IncX3 plasmid carrying blaNDM-7 in K. quasipneumoniae in China. Controlling the dissemination of IncX3 plasmids is important due to their potential for horizontal transfer.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Patricia Alba, Roberta Taddei, Gessica Cordaro, Maria Cristina Fontana, Elena Toschi, Paolo Gaibani, Ilaria Marani, Angelo Giacomi, Elena L. Diaconu, Manuela Iurescia, Virginia Carfora, Alessia Franco
Summary: The blaNDM-5-producing E. coli ST167 high-risk clone is emerging globally in human clinical cases, but has only been sporadically found in companion animals and has not been described in Italy. The sequence analysis revealed that the plasmid pMOL008 carrying blaNDM-5 is nearly identical to those found in human clinical cases in Italy and in colonized humans and a dog in Switzerland. The presence of carbapenem-resistance genes in the plasmid poses a public health threat and underscores the importance of the One Health approach for antimicrobial resistance surveillance.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Qiu-Yun Zhao, Jia-Hang Zhu, Run-Mao Cai, Xing-Run Zheng, Li-Juan Zhang, Man-Xia Chang, Yue-Wei Lu, Liang-Xing Fang, Jian Sun, Hong-Xia Jiang
Summary: Carbapenem resistance in Enterobacteriaceae is a major challenge in treating nosocomial infections. The bla(NDM) carbapenemase genes, expressed by NDM-producing Escherichia coli, have been found in various sources worldwide, including farm animals. This study investigates the dynamic evolution of blaNDM genetic contexts and bla(NDM)-harboring plasmids, with a focus on the role of IS26 elements in multi drug-resistant regions (MRRs) formation and the dissemination of blaNDM. The findings emphasize the importance of monitoring and limiting the transmission of NDM-producing isolates from food animals to humans.
Article
Infectious Diseases
Yukitaka Ito, Kotaro Aoki, Yoshikazu Ishii, Haruo Nakayama, Masanobu Otsuka, Naomi Kaneko, Mieko Yoshida, Kazuhiro Tateda, Hiroto Matsuse
Summary: We characterized plasmids carrying bla(NDM-5) detected in Escherichia coli isolated from a Japanese patient's infection site and stool sample. These plasmids showed similarity to plasmids previously detected in E. coli from a water sample, indicating potential spread within the Japanese community. The plasmids exhibited potential for easy transmission to different strains in the patient's intestine.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Microbiology
Hazrat Bilal, Gaojian Zhang, Tayyab Rehman, Jianxion Han, Sabir Khan, Muhammad Shafiq, Xuegang Yang, Zhongkang Yan, Xingyuan Yang
Summary: The study revealed the presence of various carbapenemases-producing K. pneumoniae strains in clinical samples from Pakistan, with a high prevalence of those carrying the bla(NDM-1) gene. These strains with different sequence types also showed some variation in cluster analysis. This study also identified IncX3 plasmids carrying the bla(NDM-1) gene for the first time in Pakistan, suggesting a potential new vehicle for disseminating bla(NDM-1) among Enterobacteriaceae due to its high transferability rate.
