Article
Infectious Diseases
Po-Han Huang, Yi-Hsiang Cheng, Wen-Yin Chen, Chih-Han Juan, Sheng-Hua Chou, Jin-Town Wang, Chien Chuang, Fu-Der Wang, Yi-Tsung Lin
Summary: This study investigated the risk factors and mechanisms for in vivo emergence of colistin resistance in CRKP, revealing colistin exposure as a significant factor predisposing to resistance. Alterations in the mgrB gene were identified as the predominant mechanism for emergent colistin resistance.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Microbiology
Aki Hirabayashi, Van Thi Thu Ha, An Van Nguyen, Son Thai Nguyen, Keigo Shibayama, Masato Suzuki
Summary: The study identified for the first time Klebsiella pneumoniae isolates outside of China carrying the tmexCD1-toprJ1 gene cluster, indicating horizontal spread of tigecycline resistance mediated by the gene has occurred in Vietnam and other countries,raising concerns about further global dissemination.
JOURNAL OF MEDICAL MICROBIOLOGY
(2021)
Article
Microbiology
Yawei Zhang, Xiaojuan Wang, Qi Wang, Hongbin Chen, Henan Li, Shuyi Wang, Ruobing Wang, Hui Wang
Summary: This study reported the emergence of two IMP-4 carbapenemase-producing K2-ST65 hypervirulent K. pneumoniae isolates that caused severe and fatal infections in clinical settings in China. These strains carrying bla(IMP) and virulence plasmids had the potential to exist for a long time without compromising their fitness, highlighting the urgent need to enhance clinical awareness and epidemiologic surveillance to prevent their dissemination.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Xinhong Han, Qiucheng Shi, Yihan Mao, Jingjing Quan, Ping Zhang, Peng Lan, Yan Jiang, Dongdong Zhao, Xueqing Wu, Xiaoting Hua, Yunsong Yu
Summary: This study investigated the mechanisms by which carbapenem-resistant Klebsiella pneumoniae acquires ceftazidime/avibactam (CZA) and tigecycline (TGC) resistance, finding that duplication of bla (KPC-2) and inactivation of ramR are main contributors to CZA and TGC resistance, respectively. This highlights the rapid evolution of resistance in CRKP clone ST4496, posing a potential threat to inpatients during antibiotic treatment.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Immunology
Lilan Sun, Lang Sun, Xue Li, Xinxin Hu, Xiukun Wang, Tongying Nie, Youwen Zhang, Xuefu You
Summary: ML-7 hydrochloride (ML-7) has been identified as a potential novel tigecycline adjuvant to combat tigecycline-resistant Klebsiella pneumoniae. ML-7 exhibits synergistic bactericidal activities with tigecycline, inhibits efflux pump function, disrupts proton motive force, reduces intracellular ATP levels, and leads to accumulation of ROS. Furthermore, transcriptomic analysis reveals the enrichment of ML-7-related genes in ABC transporters.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Jingchen Hao, Bangqin Zhang, Jiamin Deng, Yueshuai Wei, Xue Xiao, Jinbo Liu
Summary: The emergence of bla(NDM-1) and bla(KPC-2) co-producing Klebsiella pneumoniae strains in Southwest China has raised concerns due to their antimicrobial resistance and hypervirulent phenotypes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Suyeon Park, Hyunkeun Kim, Kwan Soo Ko
Summary: In hypervirulent Klebsiella pneumoniae strains, tigecycline resistance is associated with decreased virulence due to reduced production of capsular polysaccharides (CPS). Transcriptomic analyses revealed downregulation of ompK35 expression in tigecycline-resistant mutants, which overexpressed ompR and negatively regulated ompK35 expression. Tigecycline-resistant and ompR-overexpressed mutants exhibited reduced hypermucoviscosity and virulence, while deletion of ompR restored these phenotypes in tigecycline-resistant mutants.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Microbiology
Jiawei Chen, Yu Zeng, Rong Zhang, Jiachang Cai
Summary: Three clonally related carbapenem-resistant Klebsiella pneumoniae (CRKP) strains harbored the same set of antimicrobial resistance genes. Among them, one strain with a type 1 Tet(A) variant carrying a G300E mutation on a plasmid exhibited significantly elevated tigecycline resistance.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Weishuai Zhai, Yingxin Tian, Mi Lu, Muchen Zhang, Huangwei Song, Yulin Fu, Tengfei Ma, Chengtao Sun, Li Bai, Yang Wang, Dejun Liu, Ying Zhang
Summary: This study identified a clinical Klebsiella pneumoniae isolate harboring the tet(X4) gene on a non-self-transferable plasmid. The plasmid could be cotransferred with another conjugative plasmid to Escherichia coli, suggesting an increasing risk of spreading tet(X4) among important pathogens.
MICROBIOLOGY SPECTRUM
(2022)
Article
Infectious Diseases
Rezvan Goodarzi, Mohammadreza Arabestani, Mohmmad Yousef Alikhani, Fariba Keramat, Babak Asghari
Summary: In this study, it was found that both AcrAB-TolC and OqxAB efflux pumps may play an important role in tigecycline resistance in K. pneumoniae isolates. The emergence of the ST11 molecular type of resistant isolates should be monitored in hospitals to identify factors leading to tigecycline resistance. Limited studies have been conducted on the mechanisms of resistance of K. pneumoniae strains to tigecycline, especially in developing countries like Iran.
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES
(2021)
Article
Infectious Diseases
Cecile Emeraud, Aba Mahamat, Agnes B. Jousset, Sandrine Bernabeu, Tania Goncalves, Camille Pommier, Delphine Girlich, Aurelien Birer, Christophe Rodriguez, Jean-Michel Pawlotsky, Thierry Naas, Remy A. Bonnin, Laurent Dorte
Summary: Since 2021, the rapid spread of highly resistant NDM-14 producing Klebsiella pneumoniae has been identified in hospitals in France. This strain, primarily imported from Morocco, is highly resistant to most antimicrobials and belongs to a single clone. However, it remains susceptible to colistin, cefiderocol, and the combination of aztreonam/avibactam.
Article
Microbiology
Otavio Hallal Ferreira Raro, Patrice Nordmann, Manuel Dominguez Pino, Jacqueline Findlay, Laurent Poirel
Summary: There is an increasing occurrence of multidrug-resistant (MDR) and hypervirulent (hv) Klebsiella pneumoniae (MDR-hvKp) convergent clones. These strains can cause difficult-to-treat infections and have a higher mortality rate in healthy adults. This study in Switzerland identified a rate of 9.0% K. pneumoniae with a virulence genotype, producing carbapenemase and recovered from rectal swabs, urine, and blood. The presence of alarming MDR-hvKp and MDR nonhypervirulent K. pneumoniae (MDR-nhv-Kp) isolates highlights the need for surveillance networks to track and monitor their spread.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Jose Joao Sumbana, Antonella Santona, Nader Abdelmalek, Maura Fiamma, Massimo Deligios, Alice Manjate, Jahit Sacarlal, Salvatore Rubino, Bianca Paglietti
Summary: Globally, antibiotic-resistant Klebsiella spp. cause healthcare-associated infections with high mortality rates. The study investigated the phenotypic and genetic features of Klebsiella isolates from invasive infections in Mozambique. The majority of isolates displayed multidrug resistance, with ESBL-producing strains carrying various beta-lactamase genes. The study also identified susceptible hypervirulent Klebsiella pneumoniae isolates associated with severe infections. The findings highlight the importance of surveillance strategies to prevent the emergence of multidrug-resistant hypervirulent Klebsiella pneumoniae.
Article
Infectious Diseases
Sotiria Fergadaki, Georgios Renieris, Nikolaos Machairas, Labros Sabracos, Dionyssia-Irene Droggiti, Evangelos Misiakos, Evangelos J. Giamarellos-Bourboulis
Summary: Experimental studies demonstrated that tigecycline alone or in combination effectively prolongs survival and reduces bacterial load in KPC-Kp infections in mice.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Chemistry, Medicinal
Shiyi Shi, Mengxin Xu, Yining Zhao, Luozhu Feng, Qi Liu, Zhuocheng Yao, Yao Sun, Tieli Zhou, Jianzhong Ye
Summary: The study aimed to clarify the synergistic antibacterial activity of the combination of tigecycline and rifampicin, and investigated its impact on mutational resistance development and efficacy in an in vivo model using Galleria mellonella. Through a checkerboard test, it was found that the combination showed synergistic antibacterial activity against carbapenem-resistant Klebsiella pneumoniae. The study establishes a paradigm for evaluating drug-resistant mutant suppression in antimicrobial combination therapy.
ACS INFECTIOUS DISEASES
(2023)