Article
Chemistry, Medicinal
Clyde A. Smith, Nichole K. Stewart, Marta Toth, Pojun Quan, John D. Buynak, Sergei B. Vakulenko
Summary: The widespread presence of carbapenem-hydrolyzing beta-lactamases in Gram-negative bacteria has reduced the effectiveness of carbapenem antibiotics, limiting treatment options. In the Enterobacteriaceae family, production of class D beta-lactamases, specifically from the OXA-48-type family, is a major mechanism of resistance. This study evaluates a novel carbapenem, NA-1-157, which shows promise in treating infections caused by OXA-48-producing bacterial pathogens.
ACS INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Thongpan Leangapichart, Kamonwan Lunha, Jatesada Jiwakanon, Sunpetch Angkititrakul, Josef D. Jarhult, Ulf Magnusson, Marianne Sunde
Summary: The study found that approximately 50% of healthy pigs and humans in Thailand were colonized by Klebsiella pneumoniae complex (KP). Some KP clones were shared between pigs and humans, suggesting the possibility of zoonotic transmission.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Minjeong Kang, Tae-Rim Choi, Soyeon Ahn, Hee Young Heo, Hyerim Kim, Hye Soo Lee, Yoo Kyung Lee, Hwang-Soo Joo, Philip S. Yune, Wooseong Kim, Yung-Hun Yang
Summary: Bacteria can evade antibiotics through acquiring resistance genes and switching to a dormant state. Our study found that the cold-adapted bacterium Pseudomonas sp. B14-6 showed resistance and tolerance to aminoglycosides, and this ability appears to be related to reduced metabolic activity.
Article
Infectious Diseases
Carolina Venditti, Ornella Butera, Marcello Meledandri, Maria Pia Balice, Giulio Cesare Cocciolillo, Carla Fontana, Silvia D'Arezzo, Chiara De Giuli, Mario Antonini, Alessandro Capone, Francesco Messina, Carla Nisii, Antonino Di Caro
Summary: The study analyzed ceftazidime-avibactam-resistant KPC-producing Klebsiella pneumoniae strains collected from 31 patients in six hospitals in Rome, revealing mutations in KPC variants within the bla(KPC-3) gene and the presence of high-risk clones (ST512, 101, and 307). The findings highlight concerns over the potential selection of a multidrug-resistant phenotype in strains showing resistance to both ceftazidime-avibactam and carbapenems.
CLINICAL MICROBIOLOGY AND INFECTION
(2021)
Article
Pharmacology & Pharmacy
Constanza Nunez, Annegrett Palavecino, Ivan A. Gonzalez, Paulina Dreyse, Christian Erick Palavecino
Summary: A total of 118 clinical isolates of K. pneumoniae were characterized, showing a higher frequency of virulence genes in ESBL strains and enhanced virulence in K2+, ybtS+, and allS+ genotypes. All strains were sensitive to aPDI with PSIR-3 and the aPDI was improved when combined with Cfx, with a synergistic effect observed.
Article
Multidisciplinary Sciences
Shymaa Enany, Samira Zakeer, Aya A. Diab, Usama Bakry, Ahmed A. Sayed
Summary: In this study, the whole genome sequencing of Klebsiella pneumoniae ST627 was conducted, revealing the presence of antimicrobial resistance and virulence genes. Plasmids, phage regions, and CRISPR arrays were also identified and analyzed. The results highlight the critical role of mobile genetic elements in the dissemination of antibiotic resistance in ST 627.
Article
Microbiology
Chunmei Shen, Ying Shen, Hui Zhang, Maosuo Xu, Leqi He, Jingbo Qie
Summary: The study reveals a close correlation between antibiotic resistance and bacterial metabolic changes, which could facilitate antibiotic drug development and identify potential key survival proteins.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Immunology
Jun Li, Mengli Tang, Zhaojun Liu, Fengjun Xia, Changhang Min, Yongmei Hu, Haichen Wang, Mingxiang Zou
Summary: This study investigated the molecular and clinical characteristics of hypervirulent Klebsiella pneumoniae (hvKP) in urinary tract infections (UTIs). The results showed that hvKP had a higher resistance rate and were more likely to cause complicated UTIs. The K64 hvKP isolates exhibited diversity in serotypes and genetic environments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Lin Gan, Chao Yan, Jinghua Cui, Guanhua Xue, Hanyu Fu, Bing Du, Hanqing Zhao, Junxia Feng, Yanling Feng, Zheng Fan, Pan Mao, Tongtong Fu, Ziying Xu, Shuheng Du, Shiyu Liu, Rui Zhang, Qun Zhang, Nannan Li, Xiaohu Cui, Xiaoran Li, Yao Zhou, Lei Huang, Jing Yuan
Summary: Klebsiella pneumoniae, a common cause of nosocomial and community-acquired infections, including pneumonia and pyogenic liver abscess, has little known about its population structure. This study investigated the prevalence and characteristics of K. pneumoniae isolates from carriers, pyogenic liver abscess patients, and pneumonia patients. The isolates from different sources had their own sequence types and genomic features. The isolates from liver abscess patients carried more virulence factors, while those from pneumonia patients harbored more resistance genes and replicons. The isolates from liver abscesses also showed higher virulence. The findings suggest that isolates from different sources have distinct genomic features and may infect different patients via different sources.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Catarina Ferreira, Santosh K. Bikkarolla, Karolin Frykholm, Saga Pohjanen, Margarida Brito, Catarina Lameiras, Olga C. Nunes, Fredrik Westerlund, Celia M. Manaia
Summary: The study tracked carbapenem resistance transmission by Klebsiella pneumoniae in a healthcare facility, finding evidence of vertical transmission of resistance genes among patient isolates.
Article
Infectious Diseases
Jingxuan Zhu, Ye Chen, Xuejing Yang
Summary: This study investigated the antibiotic resistance and molecular characteristics of CRKP strains isolated in Zhejiang Province, China, revealing that CRKP isolates in ICU showed significantly higher resistance rates than those in general ward patients, 50 strains carried the blaKPC gene, and pulmonary disease may affect the prognosis of CRKP infection.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Mitra Ahmadi, Reza Ranjbar, Payam Behzadi, Taher Mohammadian
Summary: This study identified and characterized clinical strains of Klebsiella pneumoniae, revealing a high prevalence of antibiotic resistance and virulence genes. The study highlights the significant pathogenic potential of isolated pathotypes of K. pneumoniae.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2022)
Article
Microbiology
Kathleen Klaper, Jens Andre Hammerl, Jorg Rau, Yvonne Pfeifer, Guido Werner
Summary: The study investigated 94 Klebsiella spp. isolates from different animal and food sources in Germany, revealing genetic diversity. ESBL genes and iron carrier (iuc) genes were detected in the isolates. The siderophore aerobactin (iuc lineage 3) was found to be more prevalent in K. pneumoniae strains isolated from pigs.
Article
Plant Sciences
Arjun Upadhaya, Sudha G. C. Upadhaya, Robert Brueggeman
Summary: A diverse population of wheat stem rust exists in the Pacific Northwest region of the United States, and novel virulence gene combinations that could overcome existing resistances have been found. This study reports the first worldwide isolates of Puccinia graminis f. sp. tritici that are virulent on both Rpg1 and RMRL, representing the most virulent strains of this pathogen reported on barley.
Review
Microbiology
Negar Narimisa, Forough Goodarzi, Shirin Bavari
Summary: This study evaluated the prevalence of colistin resistance of K. pneumoniae isolates in Iran, finding an increased resistance rate. The results showed a colistin resistance prevalence of 6.9%, with subgroup analysis indicating a recent increase. Furthermore, a strong association between carbapenem-producing K. pneumoniae and increased colistin resistance was observed.
ANNALS OF CLINICAL MICROBIOLOGY AND ANTIMICROBIALS
(2022)
Article
Microbiology
Nawarat Somprasong, Jinhee Yi, Carina M. Hall, Jessica R. Webb, Jason W. Sahl, David M. Wagner, Paul Keim, Bart J. Currie, Herbert P. Schweizer
Summary: Burkholderia cepacia complex (Bcc) and Burkholderia pseudomallei complex (Bpc) species are typically multidrug resistant, with dominant resistance mechanisms mediated by efflux pumps of the RND family. Comparative bioinformatic and functional studies suggest that RND pump-based resistance mechanisms are conserved in Burkholderia, providing a basis for adopting a uniform RND efflux pump nomenclature.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Colin Wood, Jason Sahl, Sara Maltinsky, Briana Coyne, Benjamin Russakoff, David Panisello Yague, Jolene Bowers, Talima Pearson
Summary: Existing molecular assays for Staphylococcus aureus detection lack sensitivity and specificity. The newly developed qPCR assay SaQuant shows high sensitivity and specificity, with potential for accurate detection and quantification of S. aureus. It has the ability to better understand the dynamics of prevalence and transmission in community settings.
Article
Immunology
Voahangy Andrianaivoarimanana, David M. Wagner, Dawn N. Birdsell, Birgit Nikolay, Faniry Rakotoarimanana, Lovasoa N. Randriantseheno, Amy J. Vogler, Jason W. Sahl, Carina M. Hall, Nawarat Somprasong, Simon Cauchemez, Herbert P. Schweizer, Harimahefa Razafimandimby, Christophe Rogier, Minoarisoa Rajerison
Summary: This study retrospectively characterized a pneumonic plague outbreak in Madagascar, finding that the infection was caused by a Yersinia pestis strain resistant to streptomycin. The outbreak occurred during funeral practices and all patients recovered after antimicrobial therapy.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Public, Environmental & Occupational Health
Beza Ramasindrazana, Mamionah N. J. Parany, Fanohinjanaharinirina Rasoamalala, Mercia Rasoanoro, Soloandry Rahajandraibe, Amy J. Vogler, Jason W. Sahl, Voahangy Andrianaivoarimanana, Minoarisoa Rajerison, David M. Wagner
Summary: Plague is a re-emerging zoonotic disease of public health concern in Madagascar, where different subtypes of Y. pestis co-occur. High seroprevalence of Y. pestis in local rodent populations suggests potential resistance development. Further research on genomic characterization of Y. pestis strains in endemic regions is needed for better understanding of its phylogeography.
ZOONOSES AND PUBLIC HEALTH
(2022)
Article
Biotechnology & Applied Microbiology
Carina M. Hall, Anthony L. Baker, Jason W. Sahl, Mark Mayo, Holger C. Scholz, Mirjam Kaestli, James Schupp, Madison Martz, Erik W. Settles, Joseph D. Busch, Lindsay Sidak-Loftis, Astrid Thomas, Lisa Kreutzer, Enrico Georgi, Herbert P. Schweizer, Jeffrey M. Warner, Paul Keim, Bart J. Currie, David M. Wagner
Summary: Distinct Burkholderia strains were isolated from soil samples collected in northern Australia, and were identified as two novel species within the Burkholderia pseudomallei complex. These species, named Burkholderia mayonis sp. nov. and Burkholderia savannae sp. nov., display unique genomic characteristics and biochemical profiles. The discovery of these new species expands our understanding of this significant bacterial complex.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Genetics & Heredity
Marcus de Melo Teixeira, Jason E. Stajich, Jason W. Sahl, George R. I. I. I. I. I. I. Thompson, Rachel B. Brem, Claire A. Dubin, Austin Blackmon, Heather L. Mead, Paul Keim, Bridget M. Barker
Summary: This study sequenced and annotated the genome of the Coccidioides posadasii Silveira strain using high-quality sequencing technology, providing a foundation for future genetic studies and improving understanding of the chromosomal structure and function of this strain.
G3-GENES GENOMES GENETICS
(2022)
Article
Genetics & Heredity
Charles Hall Davis Williamson, Nathan E. Stone, Amalee E. Nunnally, Chandler C. Roe, Adam J. Vazquez, Samantha A. Lucero, Heidie Hornstra, David M. Wagner, Paul Keim, Maja Rupnik, Sandra Janezic, Jason William Sahl
Summary: This study explores the genomic diversity of Clostridioides difficile and discovers new cryptic species in soil and water samples. These species may pose challenges for clinical detection as they may not amplify in PCR diagnostics. Additionally, the study identifies antimicrobial resistance and metabolic diversity in these isolates.
MICROBIAL GENOMICS
(2022)
Article
Medicine, General & Internal
Brooke L. Deatherage Kaiser, Dawn N. Birdsell, Janine R. Hutchison, Johanna Thelaus, Sarah C. Jenson, Voahangy Andrianaivoarimanana, Mona Bystroem, Kerstin Myrtennaes, Ryelan F. McDonough, Roxanne D. Nottingham, Jason W. Sahl, Herbert P. Schweizer, Minoarisoa Rajerison, Mats Forsman, David S. Wunschel, David M. Wagner
Summary: Antimicrobial resistance is a growing concern, and we studied the proteins associated with Yersinia pestis and Francisella tularensis strains to identify potential protein signatures for antimicrobial resistance (AMR). We found that protein expression was significantly influenced by AMR, even in the absence of antibiotics in growth media. Additionally, the abundance of specific proteins involved in metabolic pathways and biological functions was altered in AMR strains, regardless of species or resistance mechanism. These features could be used to develop novel approaches for identifying AMR phenotypes.
FRONTIERS IN MEDICINE
(2022)
Article
Infectious Diseases
Nathan E. Stone, Carina M. Hall, Marielisa Ortiz, Shelby M. Hutton, Ella Santana-Propper, Kimberly R. Celona, Charles H. D. Williamson, Nicole Bratsch, Luis G. V. Fernandes, Joseph D. Busch, Talima Pearson, Sarai Rivera-Garcia, Fred Soltero, Renee Galloway, Jason W. Sahl, Jarlath E. Nally, David M. Wagner
Summary: This study investigated the diversity and distribution of Leptospira bacteria in the environment in Puerto Rico. The results showed that diverse and pathogenic Leptospira were abundant and widespread, with some lineages persisting in soil for over a year. This research will contribute to improving the monitoring and diagnosis of leptospirosis.
PLOS NEGLECTED TROPICAL DISEASES
(2022)
Article
Immunology
Lisa J. Speiser, Sabirah Kasule, Carina M. Hall, Jason W. Sahl, David M. Wagner, Chris Saling, Amy Kole, Andrew J. Meltzer, Victor Davila, Robert Orenstein, Thomas Grys, Erin Graf
Summary: Melioidosis, caused by Burkholderia pseudomallei, is a highly lethal infection with a particularly high fatality rate if left untreated or undiagnosed. It is endemic to Asia, Australia, South America, and the Caribbean, but the number of cases reported in the United States is increasing. This study highlights the rare occurrence of melioidosis presenting as a mycotic aneurysm in the United States and emphasizes the need for diagnostic awareness and epidemiological concerns.
OPEN FORUM INFECTIOUS DISEASES
(2022)
Article
Veterinary Sciences
Camila Hamond, Karen LeCount, Ellie J. Putz, Darrell O. Bayles, Patrick Camp, Marga G. A. Goris, Hans van der Linden, Nathan E. Stone, Linda K. Schlater, Jason W. Sahl, David M. Wagner, Jarlath E. Nally
Summary: This study reports the isolation of L. borgpetersenii serovar Tarassovi from the urine of a dairy cow in the U.S. for the first time. The isolate was characterized using whole-genome sequencing, serotyping, MALDI, and immunoblotting. This finding highlights the importance of culture, genotyping, and serotyping for accurate classification of leptospires and the design of effective vaccine and diagnostic strategies.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Carina M. Hall, Daniel Romero-Alvarez, Madison Martz, Ella Santana-Propper, Lora Versluis, Laura Jimenez, Abdelghafar Alkishe, Joseph D. Busch, Trevor Maness, Jonathan Stewart, Tom Sidwa, Jay E. Gee, Mindy G. Elrod, Zachary Weiner, Alex R. Hoffmaster, Jason W. Sahl, Johanna S. Salzer, A. Townsend Peterson, Amanda Kieffer, David M. Wagner
Summary: The study found that the risk of acquiring melioidosis from the environment in the continental U.S. is low, even at locations where locally acquired cases have occurred.
Article
Microbiology
Tove Hoffman, Andreas Sjodin, Caroline Ohrman, Linda Karlsson, Ryelan Francis McDonough, Jason W. Sahl, Dawn Birdsell, David M. Wagner, Laura G. Carra, Peter Wilhelmsson, John H-O Pettersson, Christos Barboutis, Jordi Figuerola, Alejandro Onrubia, Yosef Kiat, Dario Piacentini, Thomas G. T. Jaenson, Per-Eric Lindgren, Sara Moutailler, Thord Fransson, Mats Forsman, Kenneth Nilsson, Ake Lundkvist, Bjorn Olsen
Summary: The study investigates the dispersal and co-occurrence of Francisella and spotted fever group Rickettsia in ticks infesting migrating birds in the African-Western Palaearctic region. The results suggest that ticks carrying Francisella and SFGR are dispersed by northbound migratory birds in the region. The most common tick species, Hyalomma rufipes, has a high prevalence of Francisella and may have a dual endosymbiosis with Midichloria to meet its nutritional requirements.
Article
Microbiology
Michael J. Sikorski, Tracy H. Hazen, Sachin N. Desai, Susana Nimarota-Brown, Siaosi Tupua, Michelle Sialeipata, Savitra Rambocus, Danielle J. Ingle, Sebastian Duchene, Susan A. Ballard, Mary Valcanis, Sara Zufan, Jianguo Ma, Jason W. Sahl, Mailis Maes, Gordon Dougan, Robert E. Thomsen, Roy M. Robins-Browne, Benjamin P. Howden, Take K. Naseri, Myron M. Levine, David A. Rasko
Summary: In this study, the population structure, evolutionary origins, and genomic features of S. Typhi associated with decades of endemic typhoid fever in Samoa were characterized using whole genome sequencing and comparative genomics analyses. Rare S. Typhi population in Samoa that likely emerged around the early 1970s and evolved into sublineages that are presently dominant were identified. The dominance of these endemic genotypes in Samoa is not readily explained by genomic content or widespread acquisition of antimicrobial resistance. These data establish the necessary framework for future genomic surveillance of S. Typhi in Samoa for public health benefit.
Article
Microbiology
Carina M. M. Hall, Nawarat Somprasong, Johannah P. P. Hagen, Roxanne Nottingham, Jason W. W. Sahl, Jessica R. R. Webb, Mark Mayo, Bart J. J. Currie, Yuwana Podin, David M. M. Wagner, Paul Keim, Herbert P. P. Schweizer
Summary: Cefiderocol is a siderophore cephalosporin designed for treating infections caused by beta-lactam and multidrug-resistant Gram-negative bacteria. Resistance in clinical B. pseudomallei isolates from Australia is caused by an uncharacterized mechanism. The PiuA outer membrane receptor plays a major role in cefiderocol nonsusceptibility in isolates from Malaysia.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)