Article
Microbiology
Pierre-Etienne Marcoux, Sarah B. Girard, Kim C. Fournier, Catherine A. Tardif, Ariane Gosselin, Steve J. Charette
Summary: This study reveals the fusion process between the pAsa5 plasmid and the complete version of the pAsa8 plasmid. The insertion of the pAsa8 transposon into pAsa5 in a certain strain appears to be an abnormal event compared to the fusion of the two full-length plasmids. The fusion is mediated by a 22-nucleotide sequence present in both plasmids. Furthermore, the study shows that pAsa8 can be introduced into new strains through conjugation and fusion with pAsa5 can be detected.
Article
Infectious Diseases
Kim C. Fournier, Valerie E. Paquet, Sabrina A. Attere, Judith Farley, Helene Marquis, Hubert Gantelet, Christian Ravaille, Antony T. Vincent, Steve J. Charette
Summary: New variants of the pRAS3 plasmid were discovered, along with a genetic element similar to one found in a swine pathogen. Despite differences between plasmids, no specific trend in tetracycline resistance was observed. Furthermore, Blast analysis revealed the widespread distribution of pRAS3 plasmids in other bacterial species.
Article
Infectious Diseases
Pierre-Etienne Marcoux, Sabrina A. Attere, Valerie E. Paquet, Maude F. Paquet, Sarah B. Girard, Judith Farley, Michel Frenette, Antony T. Vincent, Steve J. Charette
Summary: We discovered a plasmid in Aeromonas salmonicida subsp. salmonicida, called pAsa-2939, which carries antibiotic resistance genes. This 67 kb plasmid displays unique features and contains a transposon with an unusual organization. The transposon carries a catB3 gene that has not been found in this bacterial species before. The plasmid can be transferred into Aeromonas hydrophila, but not into Escherichia coli.
Review
Multidisciplinary Sciences
Antony T. Vincent, Nava Hosseini, Steve J. Charette
Summary: This literature review provides an overview of the plasmidome of Aeromonas salmonicida, focusing on antibiotic resistance genes, virulence factors, and cryptic plasmids with unknown biological functions.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Article
Virology
Nava Hosseini, Valerie E. Paquet, Mahdi Chehreghani, Sylvain Moineau, Steve J. Charette
Summary: Aquaculture is facing antibiotic resistance issues in fighting pathogenic bacteria, with phage therapy being a potential alternative. A specific combination of four phages has shown effectiveness against A. salmonicida subsp. salmonicida, but strains with Prophage 3 exhibit reduced sensitivity. This highlights the impact of prophage content on the efficacy of phage therapy.
Article
Microbiology
Pierre-Etienne Marcoux, Antony T. Vincent, Marie-Ange Massicotte, Valerie E. Paquet, Emilie J. Doucet, Nava Hosseini, Melanie V. Trudel, Gabriel Byatt, Mathilde Laurent, Michel Frenette, Steve J. Charette
Summary: European strains of Aeromonas salmonicida subsp. salmonicida are more prone to losing the TTSS locus compared to Canadian strains. A cluster of genes encoding putative proteins with DNA binding capacity and phage proteins was found to be almost perfectly correlated with the susceptibility of different strains to lose the locus. This discovery opens up new opportunities for studying the thermosensitivity of pAsa5.
Article
Fisheries
Emmanuelle Moreau, Lionel Pineau, Florine Bachelet, Antoine Rostang, Kenny Oberle, Segolene Calvez
Summary: This study aimed to develop a method of infectious challenge in large Rainbow trout by immersion, simulating natural infection conditions. The results showed that a 24-hour contact period led to infection in all fish with a mortality rate of 53.25%. The infected fish developed acute infection symptoms and lesions similar to furunculosis, and antibodies against the bacterium were produced 4 weeks after challenging compared to the non-challenged group.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Sarah B. Girard, Valerie E. Paquet, Steve J. Charette
Summary: Aeromonas salmonicida subspecies salmonicida, a fish pathogen, expresses various virulence factors such as an A-layer, lipases and proteases during infection. This study optimized protocols for liquid tests to quickly and accurately determine the presence of these virulence factors. The new protocols provide opportunities for quicker characterization and more precise results for A. salmonicida subsp. salmonicida strains.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Qiang Fu, Hao Zhang, Yuqing Li, Pei Zhang, Chengbin Gao, Jie Li, Xingchun Li, Min Cao, Chao Li
Summary: This study conducted transcriptome analysis in Atlantic salmon head kidney following the administration of ASM vaccination, revealing genes, their expression patterns, and pathways involved in immune responses. The results showed significant up-regulation and down-regulation of genes post vaccination, with many differentially expressed genes involved in cell adhesion, cytokine-cytokine receptor interaction, viral infection, and signaling pathway related to immune responses.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Environmental Sciences
Jeff Gauthier, Helene Marquis, Valerie E. Paquet, Steve J. Charette, Roger C. Levesque, Nicolas Derome
Summary: Aeromonas salmonicida subsp. salmonicida is a Gram-negative bacterium causing furunculosis in fish, but in vivo pathogenicity data is currently lacking. Using a standardized furunculosis challenge model with a prototype strain like A. salmonicida subsp. salmonicida 890054 can provide valuable information, and computational analysis of the genome can reveal important insights.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Fisheries
Yongwei Yan, Yaokuan Liu, Zhaolan Mo, Jie Li, Shulan Liu, Ye Gao, Guiyang Li, Jie Li
Summary: A developed A. salmonicida subsp. masoucida inactivated vaccine for turbot has been proven to be effective in protecting the fish for at least 24 weeks under both laboratory and field conditions, with the emulsified vaccine showing better results than the water based vaccine. The vaccine not only reduces the infection probability by other pathogens or parasites, but also promotes fish growth without severe side-effects.
Article
Fisheries
Yibin Yang, Tao Mao, Bin Xu, Xia Zhu, Lin Zhang, Ying Huang, Hongyu Zhang, Jin Xu, Xiaohui Ai
Summary: This study investigated the characteristics of a dominant strain wx3 isolated from sick red swamp crayfish and analyzed the immune response of the crayfish under stress. The whole genome analysis confirmed that wx3 is the pathogen Aeromonas salmonicida subsp. Masoucida, which causes red swamp crayfish head rot. The study also found that wx3 is highly sensitive to certain antibiotics and that infected crayfish show a strong immune response.
Article
Fisheries
Asmaa K. Al-Mokaddem, Dalia A. Abdel-moneam, Reham A. Ibrahim, Mona Saleh, Mohamed Shaalan
Summary: Aeromonas salmonicida was isolated for the first time from Mugil carinatus fish in Suez Gulf, Egypt. The bacteriological, molecular and histopathological methods were used to identify the fish samples. The isolated A. salmonicida strains were confirmed through biochemical identification, molecular identification and histopathological analysis. The study also revealed the pathological lesions caused by A. salmonicida infection.
AQUACULTURE REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Sabrina A. Attere, Cynthia Gagne-Thivierge, Valerie E. Paquet, Gabrielle R. Leduc, Antony T. Vincent, Steve J. Charette
Summary: Except for Aeromonas salmonicida, which includes both psychrophilic and mesophilic subspecies, all 36 known species of the genus Aeromonas are mesophilic. In recent years, an increasing number of mesophilic A. salmonicida strains have been discovered, with only A. salmonicida subsp. pectinolytica being officially classified as a mesophilic subspecies. Most mesophiles have been isolated in hot countries. This study presents the characterization of two new mesophilic isolates from Quebec, Canada, which is the first time mesophilic strains from different countries are found to be so similar.
Article
Infectious Diseases
Manuel Soto-Davila, Setu Chakraborty, Javier Santander
Summary: This study evaluated the gene expression of A. salmonicida in fish infection studies and identified multiple reliable reference genes. The use of multiple reference genes and validation helps to minimize variability in qPCR studies.
INFECTION GENETICS AND EVOLUTION
(2022)
Article
Evolutionary Biology
Luca Freschi, Antony T. Vincent, Julie Jeukens, Jean-Guillaume Emond-Rheault, Irena Kukavica-Ibrulj, Marie-Josee Dupont, Steve J. Charette, Brian Boyle, Roger C. Levesque
GENOME BIOLOGY AND EVOLUTION
(2019)
Article
Genetics & Heredity
Monique Turmel, Christian Otis, Antony T. Vincent, Claude Lemieux
MITOCHONDRIAL DNA PART B-RESOURCES
(2020)
Article
Genetics & Heredity
Monique Turmel, Antony T. Vincent, Christian Otis, Claude Lemieux
MITOCHONDRIAL DNA PART B-RESOURCES
(2020)
Article
Multidisciplinary Sciences
Andres M. Rueda, Yossef Lopez de los Santos, Antony T. Vincent, Myriam Letourneau, Ines Hernandez, Clara Sanchez, Daniel Molina, Sonia A. Ospina, Frederic J. Veyrier, Nicolas Doucet
Review
Multidisciplinary Sciences
Antony T. Vincent, Nava Hosseini, Steve J. Charette
Summary: This literature review provides an overview of the plasmidome of Aeromonas salmonicida, focusing on antibiotic resistance genes, virulence factors, and cryptic plasmids with unknown biological functions.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Article
Microbiology
Frederic J. Veyrier, Cecilia Nieves, Louise H. Lefrancois, Hana Trigui, Antony T. Vincent, Marcel A. Behr
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Virology
Gabrielle R. Leduc, Valerie E. Paquet, Antony T. Vincent, Steve J. Charette
Summary: Aeromonas salmonicida strains cause bacterial infections in aquaculture, and study shows that a phage, T7-Ah, can infect various strains of this bacterium, potentially offering a treatment option for these infections.
ARCHIVES OF VIROLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Juan F. Guerra Maldonado, Antony T. Vincent, Martin Chenal, Frederic J. Veyrier
Article
Microbiology
Pierre-Etienne Marcoux, Antony T. Vincent, Marie-Ange Massicotte, Valerie E. Paquet, Emilie J. Doucet, Nava Hosseini, Melanie V. Trudel, Gabriel Byatt, Mathilde Laurent, Michel Frenette, Steve J. Charette
Summary: European strains of Aeromonas salmonicida subsp. salmonicida are more prone to losing the TTSS locus compared to Canadian strains. A cluster of genes encoding putative proteins with DNA binding capacity and phage proteins was found to be almost perfectly correlated with the susceptibility of different strains to lose the locus. This discovery opens up new opportunities for studying the thermosensitivity of pAsa5.
Article
Microbiology
Antony T. Vincent, Steve J. Charette
Summary: Aeromonas salmonicida, a bacterial species known as a feared pathogen in fish farming, is not exclusively aquatic. It includes psychrophilic and mesophilic strains, and the study of its dual lifestyle is becoming increasingly important and relevant for speciation research.
Article
Infectious Diseases
Lucie Galiot, Xavier C. Monger, Antony T. Vincent
Summary: Antibiotic resistance is a widespread issue that challenges the study and control of bacterial communities due to the mobility of resistance genes. This study investigates the relationship between insertion sequences and antibiotic resistance genes in microbiomes using two datasets. Statistically significant correlations were found in both datasets, but further research is needed to fully understand the contribution of insertion sequences to gene mobility.
Article
Microbiology
Antony T. Vincent, Laurent Intertaglia, Victor Loyer, Valerie E. Paquet, Emilie Adouane, Patrick Martin, Celine Berard, Raphael Lami, Steve J. Charette
Summary: The study identified a new genomic island, AsaGEJ2d, in sick salmon isolates from France, which is distinct from known AsaGEI variants. This suggests diversity of AsaGEIs and their distribution in different regions.
FEMS MICROBIOLOGY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Robert A. Gaultney, Antony T. Vincent, Celine Lorioux, Jean-Yves Coppee, Odile Sismeiro, Hugo Varet, Rachel Legendre, Charlotte A. Cockram, Frederic J. Veyrier, Mathieu Picardeau
NUCLEIC ACIDS RESEARCH
(2020)
Article
Microbiology
Alice Levesque, Mary Thaler, Simon J. Labrie, Catherine Marois, Antony T. Vincent, Anne-Marie Lapointe, Alexander Culley
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2020)
Article
Microbiology
Eve Bernet, Marthe Lebughe, Antony T. Vincent, Mohammad Mehdi Haghdoost, Golara Golbaghi, Steven Laplante, Annie Castonguay, Frederic J. Veyrier
Summary: The study suggests that a boron-based salt, sodium tetraphenylborate (NaBPh4), displays high bactericidal activity against Neisseria meningitidis and Neisseria gonorrhoeae, with limited impact on closely related commensal species. The drug candidate's target(s) are likely located intracellularly or within the cell envelope, and mutants with decreased susceptibility show reduced virulence. This narrow selectivity of NaBPh4 is observed to have potential therapeutic importance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
