Article
Microbiology
Amelie Lombes, Remy A. Bonnin, Frederic Laurent, Helene Guet-Revillet, Emmanuelle Bille, Vincent Cattoir, Marie-Sarah Fangous, Cecile Le Brun, Vincent Fihman, Frederic Janvier, Marie-Pierre Otto, Anais Potron, Stephane Corvec, Louise Ruffier D'Epenoux, Assaf Mizrahi, Laurent Dortet
Summary: This multicentric study in French hospitals found that 26.9% of amoxicillin-clavulanate-resistant Proteus mirabilis isolates produced the OXA-23 carbapenemase. The study also identified a screening cutoff of <11 mm for the inhibition zone diameter around the amoxicillin-clavulanate disc to accurately detect these OXA-23 producers. Whole-genome sequencing confirmed that these OXA-23 producers belonged to the same lineage known to disseminate OXA-23 or OXA-58 in P. mirabilis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Nambram Somendro Singh, Neelja Singhal, Manish Kumar, Jugsharan Singh Virdi
Summary: Waterborne E. coli resistant to AMC were susceptible to other 8-lactam/8-lactamase inhibitors, suggesting that mechanisms other than IRT point mutations may be responsible for AMC resistance in these strains. While blaTEM-1 was present, none of the strains harbored mutations that qualify as IRT, indicating the need for further research into alternative mechanisms of AMC resistance in waterborne E. coli.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Infectious Diseases
Hongying Zhu, Hong Luo, Qiaoshi Zhong, Xingwei Cao, Shumin Gu, Suqin Peng, Yanping Xiao, Yanhui Chen, Yaping Hang, Xueyao Fang, Shan Zou, Fangyou Yu, Longhua Hu
Summary: This study compared the molecular features of MSSA and MRSA isolates and found differences in sequence types, virulence genes, and antimicrobial resistance. The findings highlight the need to intensify efforts in preventing and treating MSSA infections. Further large-scale comparative epidemiological studies are necessary to understand the evolution and interaction of MSSA and MRSA populations in the future.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Mattia Pirolo, Mareliza Menezes, Peter Damborg, Alice Wegener, Birgitta Duim, Els Broens, Lisbeth Rem Jessen, Mette Schjaerff, Luca Guardabassi
Summary: The study found a correlation between oxacillin MICs and PBP mutations in MRSP strains, as well as their susceptibility to beta-lactams approved for veterinary use. Strains with low-level MRSP had an oxacillin MIC < 4 mg/L and were susceptible to cefalexin, while being less susceptible to amoxicillin/clavulanate. High oxacillin MICs were associated with mutations in multiple PBPs.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Lisa Jacobmeyer, Torsten Semmler, Ivonne Stamm, Christa Ewers
Summary: Carbapenemase-producing Acinetobacter baumannii has been identified in veterinary clinics in Germany, with different resistance gene patterns and potential transmission within the veterinary environment.
Article
Veterinary Sciences
Toloe Allahghadry, Duncan Y. K. Ng, Alireza Dibaei, Anders Miki Bojesen
Summary: The study found a high prevalence of multi-resistant Gallibacterium anatis in poultry flocks from major chicken production provinces in Iran. Identical clones of the bacteria were found to be shared among chickens within and between farms for the first time. Additionally, potential effective vaccine targets, such as the gtxA gene, were identified.
VETERINARY RESEARCH
(2021)
Article
Parasitology
Laura Mate, Mariana Ballent, Candela Canton, Carlos Lanusse, Laura Ceballos, Luis L. Ignacio Alvarez, Juan Pedro Liron
Summary: This study compared the gene expression levels of ABC transporters in sheep nematode Haemonchus contortus isolates with different susceptibility to ivermectin and found that some ABC transporter genes showed minor differences in transcription levels, but could not fully explain ivermectin resistance. Pgp-9 may be one of the most relevant candidates contributing to the multi-genic nature of ivermectin resistance.
VETERINARY PARASITOLOGY
(2022)
Article
Microbiology
Tatjana Kirtikliene, Aiste Mierauskaite, Ilona Razmiene, Nomeda Kuisiene
Summary: This study characterized 256 beta-lactam-resistant, bacteremia-causing E. coli isolates collected from 12 healthcare institutions in Lithuania in 2014 and 2018. The results showed variability and diversity in resistance and virulence genes, providing phylogenetic classification, genetic profiling, and clustering data. These findings can improve clinical control of multidrug-resistant infections in healthcare institutions and contribute to the prevention of potential outbreaks.
Article
Infectious Diseases
Huaiyu Jia, Ying Zhang, Jianzhong Ye, Wenya Xu, Ye Xu, Weiliang Zeng, Wenli Liao, Tao Chen, Jiannning Cao, Qing Wu, Tieli Zhou
Summary: The outbreak of OXA-232-producing carbapenem-resistant Klebsiella pneumoniae (CRKP) in the First Affiliated Hospital of Wenzhou Medical University was characterized by clonal transmission among ICU inpatients, low virulence, and resistance to multiple antibiotics. The use of triclosan and chlorhexidine was effective in eradicating the CRKP strains.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Immunology
Ying Li, Ming Yin, Chengju Fang, Yu Fu, Xiaoyi Dai, Wei Zeng, Luhua Zhang
Summary: Due to high resistance and virulence potential, multidrug-resistant (MDR) P. mirabilis has become a significant pathogen in catheter-associated urinary tract infections, posing a public health risk. This study investigated the resistance characteristics and virulence potential of a collection of clinical isolates. The findings highlight the urgent threat to public health posed by the prevalence of MDR P. mirabilis with high virulence potential. Evaluation: 5 out of 10.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Infectious Diseases
Kohei Ogura, Daiki Kaji, Masakazu Sasaki, Yoshihito Otsuka, Norihiko Takemoto, Tohru Miyoshi-Akiyama, Ken Kikuchi
Summary: The CC1/t1784 clone of MRSA in Japan has rapidly expanded without genomic changes.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Fisheries
Jaykumar Kiritkumar Patel, Ada Zwetlana, Dipannita Ghosh, Vineeth Rajan, Gopalan Krishnan Sivaraman, Perumal Vivekanandan, Ravikrishnan Elangovan
Summary: Aquaculture is a rapidly growing agricultural sector that provides a valuable source of animal protein. However, the presence of pathogenic microbes and antimicrobial resistance in aquaculture is a major concern. This study sequenced and analyzed the genomes of Staphylococcus spp. isolates from shrimps obtained from aquaculture farms in India, and found that most of the isolates had a close identity with human isolates, indicating a human origin of these pathogens. It highlights the importance of implementing biosecurity measures in aquaculture farms to prevent the entry and enrichment of such isolates.
Article
Agronomy
Gu Gong, Yu-Dan Zhang, Zhen-Fei Zhang, Wei-Jian Wu
Summary: This study found that intermittent feeding on susceptible varieties can reduce the virulence of herbivorous insects on resistant varieties, which can delay the progress of resistance-breaking. Therefore, careful alternation between susceptible and resistant varieties is a potential method for managing pest resistance.
Review
Public, Environmental & Occupational Health
Zixin Peng, Lin Yan, Shuran Yang, Dajin Yang
Summary: Enterococcus faecium CC17 is a hospital-adapted lineage that emerged from animal strains, with multiple antimicrobial resistance and has become the leading cause of hospital-acquired infections worldwide.
Article
Food Science & Technology
Simone de Fatima Rauber Wurfel, Denise da Fontoura Prates, Natalie Rauber Kleinubing, Joline Dalla Vecchia, Cristiane Vaniel, Louise Haubert, Odir Antonio Dellagostin, Wladimir Padilha da Silva
Summary: The study found high antimicrobial resistance and genetic diversity in Campylobacter isolates from broiler meat products in southern Brazil. Multidrug-resistant Campylobacter, especially in C. jejuni, was prevalent. Data analysis revealed that antimicrobial resistance and virulence profiles were not species-specific.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Environmental Sciences
Nicolas Francisco Fernandez-Martinez, Sheila Carcel-Fernandez, Carmen De la Fuente-Martos, Rafael Ruiz-Montero, Bernardo R. Guzman-Herrador, Rafael Leon-Lopez, Francisco Javier Gomez, Julia Guzman-Puche, Luis Martinez-Martinez, Inmaculada Salcedo-Leal
Summary: This study aimed to identify risk factors for carriage of multidrug-resistant Gram-negative bacteria (MDR-GNB) upon admission to an intensive care unit (ICU). Through a case-control study, the researchers collected sociodemographic, clinical, and microbiological data and found that liver cirrhosis was the main comorbidity associated with MDR-GNB carriage.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Microbiology
Eva Gato, Ahalieyah Anantharajah, Manuel J. Arroyo, Maria Jose Artacho, Juan de Dios Caballero, Ana Candela, Katerina Chudejova, Ignacio Pedro Constanso, Cristina Elias, Javier Fernandez, Jesus Jimenez, Pilar Lumbreras, Gema Mendez, Xavier Mulet, Patricia Perez-Palacios, Belen Rodriguez-Sanchez, Rafael Canton, Jaroslav Hrabak, Luis Mancera, Luis Martinez-Martinez, Antonio Oliver, Alvaro Pascual, Alexia Verroken, German Bou, Marina Oviano
Summary: In this study, the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales was evaluated. The method demonstrated high sensitivity and specificity, with an overall agreement rate of 92.5% relative to the reference standard. Results were obtained within 60 minutes and accuracy ranged from 83.3% to 100% among different centers. MALDI-TOF MS proves to be an outstanding tool for rapid detection of carbapenemase activity in clinical microbiology laboratories.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Cristina Lasarte-Monterrubio, Paula Guijarro-Sanchez, Alba Belles, Juan Carlos Vazquez-Ucha, Jorge Arca-Suarez, Carlos Fernandez-Lozano, German Bou, Alejandro Beceiro
Summary: This study characterized the development of carbapenem resistance in clinical isolates of Acinetobacter junii and Acinetobacter nosocomialis due to the acquisition of a plasmid-borne bla(OXA-24/40) gene, as well as the dissemination of this gene between species of Acinetobacter for the first time. The presence of a new plasmid carrying the carbapenem-resistance gene bla(OXA-24/40) was identified in both strains, facilitating the horizontal transfer of carbapenem resistance. The identification of plasmids similar to the GR12 group carrying bla(OXA-24/40) in multiple Acinetobacter species suggests a concerning dissemination and spread of carbapenem resistance in this bacterial genus.
MICROBIOLOGY SPECTRUM
(2022)
Review
Infectious Diseases
Maria Nieves Larrosa, Andres Canut-Blasco, Natividad Benito, Rafael Canton, Emilia Cercenado, Fernando Docobo-Perez, Felipe Fernandez-Cuenca, Javier Fernandez-Dominguez, Jesus Guinea, Antonio Lopez-Navas, Miguel Angel Moreno, Ma Isabel Morosini, Ferran Navarro, Luis Martinez-Martinez, Antonio Oliver
Summary: The Spanish Antibiogram Committee presents recommendations on how cumulative antibiogram reports should be prepared in Spanish laboratories of Clinical Microbiology. This article is based on Clinical Microbiology Procedure No. 51 of the Spanish Society of Infectious Diseases and Clinical Microbiology, published in 2014, and includes modifications in clinical interpretive categories recently published by EUCAST in 2019. The objective is to establish a standardized way of preparing these reports for comparing results and conducting local or national surveillance on antimicrobial susceptibility.
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA
(2023)
Review
Infectious Diseases
Alba Rivera, Belen Vinado, Natividad Benito, Fernando Docobo-Perez, Felipe Fernandez-Cuenca, Javier Fernandez-Dominguez, Jesus Guinea, Antonio Lopez-Navas, Miguel Angel Moreno, Maria Nieves Larrosa, Antonio Oliver, Ferran Navarro
Summary: Disk diffusion is a standardized method used for guiding antimicrobial therapy. Guidelines from EUCAST, which are widely implemented in Spain, have led to the recommendations by COESANT for antimicrobial selection using the disk diffusion technique.
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA
(2023)
Article
Infectious Diseases
Isabel Machuca, Julia Guzman-Puche, E. Perez-Nadales, I Gracia-Ahufinger, A. Mendez, A. Cano, J. J. Caston, A. Dominguez, J. Torre-Cisneros, L. Martinez-Martinez
Summary: This study describes the clinical and microbiological features of a community-acquired infection caused by KPC-producing K. pneumoniae resistant to ceftazidime/avibactam. The bacteria isolates were resistant to carbapenems and ceftazidime/avibactam, but susceptible to trimethoprim-sulfamethoxazole. Whole genome sequencing revealed genetic differences and the presence of a plasmid associated with trimethoprim-sulfamethoxazole resistance.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Biotechnology & Applied Microbiology
Pablo Aja-Macaya, Soraya Rumbo-Feal, Margarita Poza, Angelina Canizares, Juan A. A. Vallejo, German Bou
Summary: A new method for extraction and enrichment of genomic MPXV DNA was developed, which significantly improved the sequencing efficiency and trustworthiness of the consensus sequences. Enriched samples contained a higher percentage of MPXV reads compared to non-enriched samples, leading to more accurate and cost-effective diagnosis of contagious diseases.
Article
Infectious Diseases
Juan Carlos Vazquez-Ucha, Isaac Alonso-Garcia, Paula Guijarro-Sanchez, Cristina Lasarte-Monterrubio, Laura Alvarez-Fraga, Arnau Cendon-Esteve, Michelle Outeda, Romina Maceiras, Andrea Pena-Escolano, Marta Martinez-Guitian, Jorge Arca-Suarez, German Bou, Alejandro Beceiro
Summary: Metallo- ,B-lactamase (MBL)-producing Enterobacterales, which are resistant to almost all ,B-lactam antibiotics, pose a significant threat due to their wide dissemination and difficulty in treatment. This study evaluated the activity of aztreonam in combination with novel ,B-lactamase inhibitors (BLIs) against clinical isolates co-producing MBLs and SBLs. The results showed that aztreonam/zidebactam, aztreonam/avibactam, and aztreonam/taniborbactam were the most effective combinations, offering promising therapeutic strategies for difficult-to-treat infections.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Albert Moreno-Mingorance, Alba Mir-Cros, Lidia Goterris, Virginia Rodriguez-Garrido, Elena Sulleiro, M. Jesus Barbera, Mireia Alberny, Yannick Hoyos-Mallecot, Vicente Descalzo, Albert Bravo, Josep Roca-Grande, Belen Vinado, Tomas Pumarola, M. Nieves Larrosa, Juan Jose Gonzalez-Lopez
Summary: We described and characterized the emerging ESBL-producing Shigella spp. associated with sexual transmission among MSM in Barcelona from 2020 to 2021, and investigated their connection with contemporaneous ESBL-producing Shigella spp. from other countries. Our findings showed an increasing trend of antimicrobial resistance, particularly the dissemination of XDR ESBL-producing S. sonnei previously reported in the UK. Additionally, we observed the first emergence and clonal dissemination of ESBL-producing and fluoroquinolone-resistant S. flexneri 2a among MSM. These results underscore the importance of international collaborative surveillance in identifying and preventing the transmission of these drug-resistant pathogens.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
T. Blanco-Martin, J. Guzman-Puche, C. Riazzo, M. Gasca-Santiyan, M. Hernandez-Garcia, R. Canton, J. Torre-Cisneros, C. Herrera, L. Martinez-Martinez
Summary: IMI carbapenemases confer an unusual resistance pattern, making them difficult to detect. Commercial molecular methods usually do not include bla(IMI) genes, contributing to the hidden dissemination of bacteria producing these enzymes. We report a plasmid-encoded IMI-6 carbapenemase in an Enterobacter ludwigii isolate, providing insights into its genetic characteristics.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemical Research Methods
C. Berengua, E. Miro, C. Gutierrez, M. Sanchez, A. Mulero, P. Ramos, M. del Cuerpo, A. Torrego, I. Garcia-Cadenas, V. Pajares, F. Navarro, R. Martino, N. Rabella
Summary: The purpose of this study was to compare the detection of HCMV in BAL fluid by viral culture and qPCR, and to establish a viral load threshold for identifying cases of pneumonitis. The results showed that 81.25% of the BAL samples had concordant results. Using a cut-off value of 1258 IU/mL of HCMV in BAL, pneumonia can be diagnosed with a sensitivity of 76% and a specificity of 100%.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Infectious Diseases
Irene Sanchez-Leon, Teresa Garcia-Martinez, Seydina M. Diene, Elena Perez-Nadales, Luis Martinez-Martinez, Jean-Marc Rolain
Summary: This study aimed to investigate heteroresistance in nine clinical isolates of Klebsiella pneumoniae producing OXA-48 and describe genomic changes in vitro. It was found that the heteroresistance in K. pneumoniae isolates was mainly attributed to insertions in the mgrB gene and point mutations in colistin resistance proteins. These findings contribute to the understanding of colistin resistance mechanisms in OXA-48-producing K. pneumoniae mutants.
Article
Biochemistry & Molecular Biology
Jaume Baldira, Juan Carlos Ruiz-Rodriguez, Adolfo Ruiz-Sanmartin, Luis Chiscano, Alejandro Cortes, Diego angeles Sistac, Roser Ferrer-Costa, Inma Comas, Yolanda Villena, Maria Nieves Larrosa, Juan Jose Gonzalez-Lopez, Ricard Ferrer
Summary: Early diagnosis and appropriate treatments are crucial in reducing mortality risk for septic patients. Existing biomarkers may not be sufficient in predicting severe organ dysfunction or elevated mortality risk. This study evaluated the predictive value of MR-proADM, PCT, CRP, and lactate for 28-day mortality in sepsis patients and those with a SOFA score =6, showing that lactate had the highest predictive ability.
Article
Health Care Sciences & Services
Miquel Angel Sastre-Femenia, Almudena Fernandez-Munoz, Maria Antonia Gomis-Font, Biel Taltavull, Carla Lopez-Causape, Jorge Arca-Suarez, Luis Martinez-Martinez, Rafael Canton, Nieves Larrosa, Jesus Oteo-Iglesias, Laura Zamorano, Antonio Oliver
Summary: A high-resolution analysis of resistance patterns, genomic epidemiology, and resistome of Pseudomonas aeruginosa in Spain over a five-year period reveals a general decrease in resistance but an increase in the proportion of XDR strains producing carbapenemases, with the high-risk clone ST235 becoming more prevalent.
LANCET REGIONAL HEALTH-EUROPE
(2023)
Article
Microbiology
Irene Sanchez-Leon, Elena Perez-Nadales, Juan Antonio Marin-Sanz, Teresa Garcia-Martinez, Luis Martinez-Martinez
Summary: This study investigated heteroresistance in 10 wild-type Klebsiella pneumoniae isolates of clinical origin. The results showed the importance of persistent variants and stable mutants in heteroresistance, and described the genomic changes observed in mutants obtained in population analysis profiling (PAP) assays.
MICROBIOLOGY SPECTRUM
(2023)