Article
Microbiology
Devin Sindeldecker, Shaurya Prakash, Paul Stoodley
Summary: This study analyzed the zone of killing (ZOK) in Pseudomonas aeruginosa biofilms using both in vitro and in silico methods. The findings revealed that achieving a specific antibiotic concentration versus time constraint (AUC) could completely eradicate bacteria in the biofilms. This suggests that appropriate antibiotic concentrations and treatment duration may be effective against antibiotic-resistant and -tolerant biofilm infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Xiaoya Wei, Jiacong Gao, Congjuan Xu, Xiaolei Pan, Yongxin Jin, Fang Bai, Zhihui Cheng, Iain L. Lamont, Daniel Pletzer, Weihui Wu
Summary: This study reveals the response mechanism of Pseudomonas aeruginosa to murepavadin and provides a promising antibiotic combination for the treatment of P. aeruginosa infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Xiaoya Wei, Jiacong Gao, Congjuan Xu, Xiaolei Pan, Yongxin Jin, Fang Bai, Zhihui Cheng, Iain L. Lamont, Daniel Pletzer, Weihui Wu
Summary: This study reveals the response mechanism of P. aeruginosa to murepavadin and provides a promising antibiotic combination for the treatment of P. aeruginosa infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Fernanda Jimenez Otero, Dianne K. Newman, Leonard M. Tender
Summary: Biofilms provide a protective environment but also present challenges to the cells living within them. Pseudomonas aeruginosa overcomes oxygen limitation by secreting redox active phenazines, which act as electron shuttles. Blocking the re-oxidation of one of these electron shuttles, pyocyanin, decreases cell survival within biofilms and enhances the effect of gentamicin in killing cells. The study highlights the importance of redox cycling of electron shuttles in P. aeruginosa biofilms.
Article
Infectious Diseases
Mariana Castanheira, Timothy B. Doyle, Cory M. Hubler, Timothy D. Collingsworth, Sean DeVries, Rodrigo E. Mendes
Summary: This study conducted a whole genome and transcriptome analysis of 213 Pseudomonas aeruginosa isolates resistant to antipseudomonal beta-lactams, revealing complex resistance mechanisms involving multiple genes.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Article
Microbiology
Xinrui Zhao, Yongxin Jin, Fang Bai, Zhihui Cheng, Weihui Wu, Xiaolei Pan
Summary: Aztreonam/avibactam is an effective drug against Metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa, a pathogen causing highly lethal infections. This study discovered a novel gene mutation, PM292, that could lead to resistance to aztreonam/avibactam and beta-lactams. Additionally, the study found that overproduction of pyocyanin increased bacterial resistance to beta-lactams.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Ashlan J. Kunz Coyne, Mohammad Alshaer, Anthony M. Casapao, Veena Venugopalan, Carmen Isache, Jason Ferreira, Christopher A. Jankowski
Summary: This study compared clinical outcomes in hospitalized patients with Pseudomonas aeruginosa pneumonia or bloodstream infection who received beta-lactam antibiotic infusions with and without the guidance of therapeutic drug monitoring. The results showed that therapeutic drug monitoring decreased the odds of treatment failure, but also increased the risk of infection-related readmissions.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Pharmacology & Pharmacy
Stef Schouwenburg, Enno D. Wildschut, M. de Hoog, Birgit C. P. Koch, Alan Abdulla
Summary: The EXPAT Kids study is designed to analyze whether current empiric dosing regimens of frequently used beta-lactam antibiotics achieve defined therapeutic target concentrations in PICU patients.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Microbiology
Xuerui Bao, Ellen Goeteyn, Aurelie Crabbe, Tom Coenye
Summary: D,L-malate enhances ciprofloxacin activity against P. aeruginosa, improving antibiotic therapy in infection environments. The choice of model system influences the outcome of the experiment, emphasizing the importance of using models that closely mimic the in vivo environment. This study confirms the potential of D,L-malate to enhance ciprofloxacin activity against P. aeruginosa-associated infections.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Sofie A. M. Dhaese, Eric A. Hoste, Jan J. De Waele
Summary: The surge in antimicrobial resistance and limited availability of new drugs has sparked interest in optimizing antibiotic dosing. For beta-lactam antibiotics, prolonged infusion has been widely adopted due to PK/PD considerations. However, merely increasing target attainment is unlikely to improve clinical outcomes. A different approach, focusing on the maximum tolerable dose (MTD), is necessary to maximize bacterial cell kill while minimizing the risk of resistance and toxicity.
Article
Biochemistry & Molecular Biology
Vidhu Agarwal, Tara Chand Yadav, Akhilesh Tiwari, Pritish Varadwaj
Summary: Antimicrobial resistance is a growing global health challenge. Bacterial infections have developed resistance against beta-lactam antibiotics through various mechanisms, including the production of novel beta-lactamase enzymes. These bacteria have also developed resistance against beta-lactamase inhibitors. Investigating the role of important residues in altering and expanding the spectrum activity of these enzymes is necessary to understand beta-lactamase-mediated resistance.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Infectious Diseases
Eric Reid, Ryan W. Walters, Christopher J. Destache
Summary: The meta-analyses demonstrate that FQ monotherapy significantly improves survival in PA bacteremia and shows similar rates of bacteriological eradication in pneumonia and skin and soft tissue infection caused by PA compared to BL monotherapy. Further research is needed to provide meaningful clinical recommendations.
Review
Biochemistry & Molecular Biology
Vidhu Agarwal, Akhilesh Tiwari, Pritish Varadwaj
Summary: Beta-lactam antibiotics are effective in treating bacterial infections, but resistance has developed due to overuse and misuse. Resistance is primarily caused by beta-lactamase enzymes that hydrolyze these antibiotics. Bacteria evolve and mutate to produce such enzymes, leading to resistance even against newly discovered antibiotics. Carbapenems are considered the last line of defense, but now resistance is also developing against this combination. This extensive review discusses the different classes of beta-lactamase enzymes, their mechanisms of action, and the role of structural elements in resistance.
CURRENT MEDICINAL CHEMISTRY
(2023)
Article
Infectious Diseases
Gianluca Morroni, Lucia Brescini, Alberto Antonelli, Vincenzo Di Pilato, Sefora Castelletti, Andrea Brenciani, Gloria D'Achille, Marina Mingoia, Eleonora Giovanetti, Simona Fioriti, Annamaria Masucci, Tommaso Giani, Andrea Giacometti, Gian Maria Rossolini, Oscar Cirioni
Summary: A surveillance study conducted in a regional hospital in Italy revealed the presence of Ceftolozane/tazobactam-resistant P. aeruginosa isolates carrying multiple resistance mechanisms acquired by various high-risk clones.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2022)
Review
Chemistry, Inorganic & Nuclear
Micha Zabiszak, Justyna Frymark, Kazuma Ogawa, Monika Skrobanska, Martyna Nowak, Renata Jastrzab, Malgorzata T. Kaczmarek
Summary: Beta-lactam antibiotics are widely used in medicine due to their strong antibacterial activity. They are produced by fungi and bacteria, and their advantages lie in their potent bactericidal activity and wide spectrum of activity. The common mode of action of beta-lactam antibiotics involves inhibiting the synthesis of the bacterial cell wall, leading to the demise of the bacterial cell.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Biology
Maria Escobar-Salom, Gabriel Torrens, Elena Jordana-Lluch, Antonio Oliver, Carlos Juan
Summary: The review focuses on bacterial envelope-targeting proteins/peptides of mammalian innate immune system and their potential therapeutic applications against multidrug-resistant 'ESKAPE' Gram-negative pathogens. It discusses experimental therapeutic approaches utilizing these immune elements and explores the possibilities of improving their bactericidal power by targeting bacterial and/or host factors. The review also identifies areas for future research to obtain successful envelope-targeting therapeutic options to combat antimicrobial resistance.
BIOLOGICAL REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Jiuxin Qu, Zhao Cai, Xiangke Duan, Han Zhang, Hang Cheng, Shuhong Han, Kaiwei Yu, Zhaofang Jiang, Yingdan Zhang, Yang Liu, Fang Bai, Yingxia Liu, Lei Liu, Liang Yang
Summary: Our study suggests that SARS-CoV-2 infection can lead to genetic adaptation of bacterial pathogens, promoting chronic infections. The adapted bacteria exhibit characteristics such as excessive biofilm formation and reduced virulence, enabling them to evade immune clearance. This highlights the importance of understanding the lung microenvironment post-virus infections and superinfection mechanism in COVID-19 patients.
CELL AND BIOSCIENCE
(2022)
Article
Microbiology
Carla Lopez-Causape, Pablo A. Fraile-Ribot, Santiago Jimenez-Serrano, Gabriel Cabot, Ester del Barrio-Tofino, M. Carmen Prado, Juana Maria Linares, Aranzazu Lopez, Adoracion Hurtado, Elena Riera, Antoni Serra, Eva Rosello, Lluis Carbo, M. Victoria Fernandez-Baca, Carmen Gallegos, Juan Saurina, Emilio Arteaga, M. Magdalena Salom, Antonia Salva, Antoni Nicolau, Fernando Gonzalez-Candelas, Inaki Comas, Antonio Oliver
Summary: Analysis of SARS-CoV-2 genomic epidemiology in the Balearic Islands, a unique setting influenced by insularity, severe social restrictions, and tourism travels, revealed B.1.1.7 and B.1.617.2/AY.X as major circulating lineages. Additionally, lineage B.1.221 was the third most prevalent in Ibiza/Formentera.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Eva Gato, Ahalieyah Anantharajah, Manuel J. Arroyo, Maria Jose Artacho, Juan de Dios Caballero, Ana Candela, Katerina Chudejova, Ignacio Pedro Constanso, Cristina Elias, Javier Fernandez, Jesus Jimenez, Pilar Lumbreras, Gema Mendez, Xavier Mulet, Patricia Perez-Palacios, Belen Rodriguez-Sanchez, Rafael Canton, Jaroslav Hrabak, Luis Mancera, Luis Martinez-Martinez, Antonio Oliver, Alvaro Pascual, Alexia Verroken, German Bou, Marina Oviano
Summary: In this study, the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) for rapid detection of carbapenemase activity in Enterobacterales was evaluated. The method demonstrated high sensitivity and specificity, with an overall agreement rate of 92.5% relative to the reference standard. Results were obtained within 60 minutes and accuracy ranged from 83.3% to 100% among different centers. MALDI-TOF MS proves to be an outstanding tool for rapid detection of carbapenemase activity in clinical microbiology laboratories.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Jing Wang, Xi Liu, Kaiwei Yu, Moxiao Liu, Jiuxin Qu, Yingxia Liu, Zhao Cai, Ke Wang, Chao Zhuo, Liang Yang, Yingdan Zhang
Summary: The co-infection of Pseudomonas aeruginosa (Pa) and Acinetobacter baumannii (Ab) in ICU patients is common but challenging to eliminate. Research suggests that antibiotic exposure reshapes the community compositions of dual-species biofilms, with different EPS structures playing roles in maintaining stability, highlighting the potential of targeting EPS structural fibers like Psi and extracellular DNA (eDNA) as a strategy for controlling polymicrobial biofilm-related infections.
ACS INFECTIOUS DISEASES
(2022)
Review
Biotechnology & Applied Microbiology
Xiaobo Liu, Bin Cao, Liang Yang, Ji-Dong Gu
Summary: Biofilm formation and biofilm-induced biodeterioration have significant impacts on the community. Cyclic dimeric guanosine monophosphate (c-di-GMP) is a key signaling molecule in bacteria, regulating various bacterial processes. Understanding the regulatory mechanisms of c-di-GMP would benefit the control of bacterial processes and the development of anti-biofilm technologies.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Biotechnology & Applied Microbiology
Yu-Ming Cai, Kai-Wei Yu, Ji-Hong Liu, Zhao Cai, Zun-Hao Zhou, Yang Liu, Tian-Fu Wang, Liang Yang
Summary: The c-di-GMP signaling pathways in P. aeruginosa are highly organized and play important roles in the transition between motile and sessile lifestyles. This study identified a phosphodiesterase, PipA, that regulates bacteriophage production, and uncovered a potential signaling circuit for biofilm treatment.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Microbiology
Tianyuan Jia, Dongjing Liu, Xianbiao Bi, Menglu Li, Zhao Cai, Jiapeng Fu, Zhi Liu, Pengyao Wu, Xue Ke, Aiqun Jia, Guoliang Zhang, Guobao Li, Liang Yang
Summary: This study reveals that the AhR ligand Pht and its vitamin K analogs can inhibit the quorum sensing mechanism of P. aeruginosa and activate immune-modulating functions, suggesting their potential as alternative treatments for infections.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Eva Armengol, Kasper Norskov Kragh, Tim Tolker-Nielsen, Josep M. Sierra, Doaa Higazy, Oana Ciofu, Miguel Vinas, Niels Hoiby
Summary: The combination of colistin and rifampicin has been found to effectively kill colistin-resistant Pseudomonas aeruginosa, suggesting it as a promising treatment for infections caused by this strain.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Immunology
Magnus Bock, Anna Marie Theut, Johan G. C. van Hasselt, Hengzhuang Wang, Kurt Fuursted, Niels Hoiby, Christian Johann Lerche, Nikolaj Ihlemann, Sabine Gill, Ulrik Christiansen, Hans Linde Nielsen, Lars Lemming, Hanne Elming, Jonas A. Povlsen, Niels Eske Bruun, Dan Hofsten, Emil L. Fosbol, Lars Kober, Martin Schultz, Mia M. Pries-Heje, Jonas Henrik Kristensen, Jens Jorgen Christensen, Flemming S. Rosenvinge, Christian Torp Pedersen, Jannik Helweg-Larsen, Niels Tonder, Kasper Iversen, Henning Bundgaard, Claus Moser
Summary: Pharmacokinetic/pharmacodynamic analyses were conducted to assess the probabilities of target attainment for oral treatments of infective endocarditis. The majority of patients achieved the target level with orally administered antibiotics, supporting the efficacy of oral step-down antibiotic treatment in patients with infective endocarditis.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Magnus Bock, Johan G. C. Van Hasselt, Franziska Schwartz, Hengzhuang Wang, Niels Hoiby, Kurt Fuursted, Nikolaj Ihlemann, Sabine Gill, Ulrik Christiansen, Niels Eske Bruun, Hanne Elming, Jonas A. Povlsen, Lars Kober, Dan E. Hofsten, Emil L. Fosbol, Mia M. Pries-Heje, Jens Jorgen Christensen, Flemming S. Rosenvinge, Christian Torp-Pedersen, Jannik Helweg-Larsen, Niels Tonder, Kasper Iversen, Henning Bundgaard, Claus Moser
Summary: This study characterized the population pharmacokinetics of linezolid in patients with infective endocarditis and found that there is a substantial interaction between linezolid and rifampicin, which increases the clearance of linezolid. Model-based simulations showed that increasing the linezolid dose can compensate for the interaction without increasing the risk of adverse effects.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Microbiology
Anne Sofie Laulund, Franziska Angelika Schwartz, Lars Christophersen, Mette Kolpen, Peter Ostrup Jensen, Henrik Calum, Niels Hoiby, Kim Thomsen, Claus Moser
Summary: Chronic wounds have compromised microcirculation and are often infested with microorganisms in biofilms, hindering antibiotic function. This study investigates whether hyperbaric oxygen therapy (HBOT) can counteract this process.
Article
Microbiology
Anne Sofie Laulund, Franziska Angelika Schwartz, Niels Hoiby, Kim Thomsen, Claus Moser
Summary: The study demonstrates that hyperbaric oxygen therapy (HBOT) can enhance the effectiveness of tobramycin in biofilms and counteract the micro-compartmentalization of biofilm pharmacokinetics through increased available tobramycin and augmented bacterial killing.
Article
Microbiology
Guohui Xiao, Zhao Cai, Qinglong Guo, Taosheng Ye, Yimin Tang, Peikun Guan, Juanjuan Zhang, Min Ou, Xiangdong Fu, Lili Ren, Minfei Yu, Zhaoqin Wang, Lei Liu, Liang Yang, Guoliang Zhang
Summary: This study aimed to determine the lung microbiota profile associated with pulmonary tuberculosis (TB) and characterize the changes during anti-TB treatment. The lung microbiota of untreated pulmonary TB patients was found to be distinct from that of healthy individuals and lung cancer patients. Anti-TB treatment significantly affected the diversity of the lung microbiota and induced the presence of antibiotic resistance genes.
MICROBIOLOGY SPECTRUM
(2022)
