Article
Infectious Diseases
Hannah Yejin Kim, Evelien Ruiter, Erwin M. Jongedijk, Hemanth Kumar Ak, Ben J. Marais, P. K. Bhavani, Shailendra Sawleshwarkar, Daan J. Touw, Jan-Willem Alffenaar
Summary: The study validated the feasibility and accuracy of a UV spectrophotometric assay using non-invasive saliva sampling for linezolid.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Microbiology
Jian Du, Jingtao Gao, Yanhong Yu, Qingfeng Li, Guanghong Bai, Wei Shu, Mengqiu Gao, Yuhong Liu, Lu Wang, Yufeng Wang, Zhongtan Xue, Fengmin Huo, Liang Li, Yu Pang
Summary: In this retrospective study in China, researchers aimed to determine the prevalence of linezolid resistance among MDR-TB patients, monitor changes in linezolid susceptibility during treatment, and explore molecular mechanisms of resistance. They found that 6.9% of patients had linezolid resistance, with a higher rate in those previously exposed to the drug. The most common genetic mutation conferring resistance was a Cys154Arg substitution in the ribosomal protein L3.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Valentina Iacuzzi, Bianca Posocco, Martina Zanchetta, Sara Gagno, Ariana Soledad Poetto, Michela Guardascione, Giuseppe Toffoli
Summary: This review critically investigates the DBS-to-plasma concentration conversion methods proposed and applied in the last decade for quantitative bioanalysis of anticancer drugs in DBS matrix, highlighting the need to address challenges in comparing drug concentrations obtained from DBS with those obtained from reference matrix to ensure treatment efficacy.
PHARMACEUTICAL RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Yuan Yuan, Jinmeng Li, Yanhong Chen, Qingshan Cai, Yingying Xu, Luting Lin, Yazhen Lang, Suhang Guo, Ruoying Zhang, Xinjun Cai
Summary: This study investigates the mechanism of linezolid-induced peripheral neuropathy in multidrug-resistant tuberculosis (MDR-TB). The results show that linezolid inhibits autophagy flux, leading to the development of peripheral neuropathy. The findings suggest a potential neuroprotective role of autophagy in preventing linezolid-induced neuropathy in MDR-TB patients.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Immunology
Johannes Eimer, Mathilde Frechet-Jachym, Damien Le Du, Eric Caumes, Najoua El-Helali, Dhiba Marigot-Outtandy, Frederic Mechai, Gilles Peytavin, Valerie Pourcher, Christophe Rioux, Yazdan Yazdanpanah, Jerome Robert, Lorenzo Guglielmetti
Summary: This study evaluated the association between linezolid trough concentrations and severe toxicity in patients treated for multidrug-resistant tuberculosis. The results showed that linezolid concentrations >2 mg/L were strongly associated with the development of severe treatment-emergent toxicity, suggesting the need for an individual risk-benefit assessment in patients with high trough concentrations.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Pharmacology & Pharmacy
Haoyue Zhang, Yuying He, Lina Davies Forsman, Jakob Paues, Jim Werngren, Katarina Niward, Thomas Schon, Judith Bruchfeld, Jan-Willem Alffenaar, Yi Hu
Summary: A population pharmacokinetic (PK) model for linezolid was developed, and a PK/pharmacodynamics (PD) threshold for successful treatment outcome was identified. The currently recommended linezolid doses were evaluated, and it was found that an AUC(0-24h)/MIC > 125 was associated with successful treatment outcomes.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Pharmacology & Pharmacy
Isadora Ritter Mueller, Gabriel Linden, Mariele Feiffer Charao, Marina Venzon Antunes, Rafael Linden
Summary: The use of dried blood spots (DBS) in therapeutic drug monitoring (TDM) has advantages such as minimally invasive collection, stabilization of drugs at room or high temperatures, and inexpensive storage. However, there are challenges related to hematocrit effects and differences between blood concentrations that need to be addressed. This article reviews recent publications on DBS sampling for TDM and discusses the opportunities and challenges in clinical applications.
EXPERT REVIEW OF CLINICAL PHARMACOLOGY
(2023)
Article
Infectious Diseases
Anastasia Ushtanit, Yulia Mikhailova, Alexandra Lyubimova, Marina Makarova, Svetlana Safonova, Alexey Filippov, Sergey Borisov, Danila Zimenkov
Summary: Linezolid resistance mainly emerged during treatment with the latest regimen, and three primary cases with linezolid resistance questioned the possible transmission of totally drug-resistant tuberculosis in the Moscow region, which requires further investigation.
Review
Pharmacology & Pharmacy
Yu Han, Xi-Ling Li, Minghui Zhang, Jing Wang, Su Zeng, Jun Zhe Min
Summary: In recent years, noninvasive sampling methods for therapeutic drug monitoring and disease diagnosis have gained increasing interest among scientific researchers. Dried saliva spot (DSS), a sampling technique for collecting dried saliva samples, has been widely used as an alternative to serum for detecting target molecules. Coupling the DSS method with a highly sensitive detection instrument improves the efficiency of preparing and analyzing biological samples. This review provides integral strategies and guidelines for dried spot methods in analyzing biological samples, focusing on DSS methods from June 2014 to March 2021. The advantages and disadvantages of key aspects of this method, including sample preparation and method validation, are discussed, along with the challenges and prospects of these methods in practical applications.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2022)
Article
Medical Laboratory Technology
Marith Francke, Laura E. J. Peeters, Dennis A. Hesselink, Sanne M. Kloosterboer, Birgit C. P. Koch, Herman Veenhof, Brenda C. M. de Winter
Summary: The article discusses important considerations for implementing DBS in clinical practice, including patient selection, blood sampling, transport, and laboratory analysis. It also shares experiences and provides suggestions on how to deal with these issues in a clinical setting.
THERAPEUTIC DRUG MONITORING
(2022)
Article
Immunology
Tasnim Hasan, Ellie Medcalf, Bern-Thomas Nyang'wa, Erica Egizi, Catherine Berry, Matthew Dodd, Salah Foraida, Medea Gegia, Mengchun Li, Fuad Mirzayev, Hannah Morgan, Ilaria Motta, Linh Nguyen, Samuel Schumacher, Tim Schlub, Greg Fox
Summary: By reviewing and meta-analyzing clinical trial data, it was found that BPaL-containing regimens with a daily dose of 600 mg linezolid showed good tolerability, which may improve the tolerability of treatment for MDR/RR-TB.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Infectious Diseases
Getu Diriba, Ayinalem Alemu, Bazezew Yenew, Habteyes Hailu Tola, Dinka Fikadu Gamtesa, Hilina Mollalign, Kirubel Eshetu, Shewki Moga, Saro Abdella, Getachew Tollera, Abebaw Kebede, Mesay Hailu Dangisso
Summary: This study aimed to estimate the prevalence of extensively drug-resistant tuberculosis (XDR-TB) and pre-extensively drug-resistant tuberculosis (pre-XDR-TB) in patients with multidrug-resistant TB (MDR-TB). A total of 64 studies involving 12,711 patients from 22 countries were included. The pooled proportion of pre-XDR-TB was 26%, while XDR-TB in MDR-TB cases was 9%. These findings highlight the substantial burden of pre-XDR-TB and XDR-TB in MDR-TB patients, emphasizing the importance of strengthening TB programs and drug resistance surveillance.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Biochemical Research Methods
Sigrid Deprez, Christophe P. Stove
Summary: The study evaluated the use of DBS-MS 500 online coupled to an LC-MS/MS system for TDM of immunosuppressants. Two methods for quantifying tacrolimus, sirolimus, everolimus, and cyclosporin A in both DBS and whole blood were developed and validated, with accurate and reproducible results. However, a hematocrit-dependent relative recovery was observed for DBS samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Review
Biotechnology & Applied Microbiology
Ayushi Nair, Alosh Greeny, Amritasree Nandan, Ranjay Kumar Sah, Anju Jose, Sathish Dyawanapelly, Vijayabhaskarreddy Junnuthula, K. Athira, Prashant Sadanandan
Summary: Conventional TB therapy faces limitations, while advanced drug delivery strategies have the potential to address these challenges. These strategies, such as multiple drug encapsulation, sustained release, targeted delivery, and reduced dosing frequency, can improve TB patient compliance and enhance therapeutic outcomes.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Infectious Diseases
Wenqiang Zhou, Wenjuan Nie, Qingfeng Wang, Wenhui Shi, Yang Yang, Qi Li, Hui Zhu, Zhongquan Liu, Yangming Ding, Yu Lu, Naihui Chu
Summary: This study aimed to find the optimal dosing regimen of linezolid in different populations. The results revealed that factors such as body weight and creatinine clearance significantly influenced the pharmacokinetics of linezolid. The study determined the optimal dosage regimens for patients with different body weights and MIC values, and found that single dosing was safer than multiple dosing.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Hematology
Anne-Grete Martson, Ana Rita da Silva Ferreira, Anette Veringa, Lei Liu, Hannah R. Wardill, Lenneke A. T. Junier, Tjip S. van der Werf, Hermie J. M. Harmsen, Marieke G. G. Sturkenboom, Lambert F. Span, Wim J. E. Tissing, Jan-Willem C. Alffenaar
Summary: This study aimed to explore the association between different stages of gastrointestinal mucositis, drug exposure, and gut microbiota. It was found that severe gastrointestinal mucositis could compromise drug absorption and a weak correlation between ciprofloxacin and citrulline concentrations was observed. Further research is needed to investigate the relationship between gastrointestinal mucositis, drug exposure, and gut microbiome.
ANNALS OF HEMATOLOGY
(2023)
Article
Infectious Diseases
Anette Veringa, Roger J. Bruggemann, Lambert F. R. Span, Bart J. Biemond, Mark G. J. de Boer, Edwin R. van den Heuvel, Saskia K. Klein, Doris Kraemer, Monique C. Minnema, Niek H. J. Prakken, Bart J. A. Rijnders, Jesse J. Swen, Paul E. Verweij, Marielle J. Wondergem, Paula F. Ypma, Nicole Blijlevens, Jos G. W. Kosterink, Tjip S. van der Werf, Jan-Willem C. Alffenaar
Summary: This study investigated whether TDM-guided voriconazole treatment is superior to standard treatment for invasive aspergillosis. The results showed no significant difference in treatment outcome and adverse reactions between the TDM and non-TDM groups, but a higher proportion of voriconazole concentrations within the acceptable range were found in the TDM group.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Medical Laboratory Technology
Sjanene Marfil, Anne-Grete Maertson, Marlous Toren-Wielema, Coretta Leer-Buter, Elisabeth. H. Scholvinck, Jan-Willem C. Alffenaar, Daan. J. Touw, Marieke G. G. Sturkenboom
Summary: Ganciclovir therapeutic drug monitoring (TDM) in 12 treatment episodes showed significant variability in both trough concentration and area under the 24-hour concentration-time curve (AUC24), both within and between individuals. Subtherapeutic concentrations were found in 30% of the treatment episodes, despite adequate valganciclovir dosing. However, a decrease in viral load was still observed. These findings highlight the importance of evaluating target concentrations and assessing the applicability of ganciclovir TDM in children.
THERAPEUTIC DRUG MONITORING
(2023)
Article
Pharmacology & Pharmacy
Haoyue Zhang, Yuying He, Lina Davies Forsman, Jakob Paues, Jim Werngren, Katarina Niward, Thomas Schon, Judith Bruchfeld, Jan-Willem Alffenaar, Yi Hu
Summary: A population pharmacokinetic (PK) model for linezolid was developed, and a PK/pharmacodynamics (PD) threshold for successful treatment outcome was identified. The currently recommended linezolid doses were evaluated, and it was found that an AUC(0-24h)/MIC > 125 was associated with successful treatment outcomes.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Elise A. Schubert, Johannes C. Alffenaar, Masego T. Johnstone, John W. Barlow, Nial J. Wheate
Summary: This study found that certain concomitant medications and cannabis constituents may be associated with the incidence of adverse events when initiating medicinal cannabis.
INTERNATIONAL JOURNAL OF PHARMACY PRACTICE
(2023)
Article
Biochemistry & Molecular Biology
Nguyen Thi Hai Yen, Nguyen Ky Anh, Rannissa Puspita Jayanti, Nguyen Ky Phat, Dinh Hoa Vu, Jong-Lyul Ghim, Sangzin Ahn, Jae-Gook Shin, Jee Youn Oh, Nguyen Phuoc Long, Dong Hyun Kim
Summary: This study investigated the plasma metabolic profiles of tuberculosis (TB) patients and TB patients with type 2 diabetes mellitus (DM) using a multimodal metabolomics and lipidomics approach. Significant differences in biological processes and biomarkers were identified between TB and TB-DM, providing insights into the pathophysiology of TB-DM and potential improvements in TB treatment. The integration of multimodal metabolomics and lipidomics provides a comprehensive understanding of the metabolic changes associated with TB-DM, enhancing our knowledge of DM comorbidity in TB infection and contributing to the development of more effective treatment.
Review
Pharmacology & Pharmacy
Prakruti S. Rao, Nisha Modi, Nam-Tien Tran Nguyen, Dinh Hoa Vu, Yingda L. Xie, Monica Gandhi, Roy Gerona, John Metcalfe, Scott K. Heysell, Jan-Willem C. Alffenaar
Summary: Quantifying drug exposure is crucial for personalized dose adjustment in tuberculosis patients. Traditional methods using serum or plasma samples for drug monitoring have logistical challenges. Less invasive and lower cost tests using alternative biomatrices can improve feasibility.
CLINICAL PHARMACOKINETICS
(2023)
Article
Infectious Diseases
D. Abraham Van den Born, Anne-Grete Martson, Anette Veringa, Nieko C. Punt, Tjip S. Van der Werf, Jan-Willem C. Alffenaar, Marieke G. G. Sturkenboom, Daan J. Touw
Summary: This study investigated the effect of inflammation on the metabolism of voriconazole and found that C-reactive protein (CRP) was the only significant covariate. The final pharmacokinetic model, which included CRP as a covariate, can be applied in clinical care.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Cindy Lau, Deborah Marriott, Jessica Bui, Melanie Figtree, Michael Gould, Adriana Chubaty, Yuen Su, Suman Adhikari, Pam Konecny, Kristi Kozierowski, Taylor Holland, Eliza Milliken, Ayesha Akram, Alexander Mcnamara, Yihui Sun, Sebastiaan Van Hal, Asad E. Patanwala, Aryan Shahabi-Sirjani, Timothy Gray, Chin Yen Yeo, Angela Netluch, Stephanie Halena, Marcelle Appay, Rozanna Alameddine, Fiona Yin, Quoc Nguyen, Mei-Yi So, Indy Sandaradura, Hannah Yejin Kim, Semun Galimam, Nicole Cerruto, Tony Lai, Timothy Gilbey, Kathryn Daveson, Stephanie E. Reuter, Jonathan Penm, Jan-Willem Alffenaar
Summary: This study aimed to evaluate linezolid toxicity in a large multicentre cohort and found that linezolid treatment-limiting toxicity remains a problem. TDM-guided dose optimisation significantly reduced the risk of toxicity and a treatment duration beyond 28 days was no longer significantly associated with toxicity.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Infectious Diseases
Joanitah Nalunjogi, Sergio Mucching-Toscano, Jean Pierre Sibomana, Rosella Centis, Lia D'Ambrosio, Jan-Willem Alffenaar, Justin Denholm, Francois-Xavier Blanc, Sergey Borisov, Edvardas Danila, Raquel Duarte, Jose-Maria Garcia-Garcia, Delia Goletti, Catherine W. M. Ong, Adrian Rendon, Tania A. Thomas, Simon Tiberi, Martin Van den Boom, Giovanni Sotgiu, Giovanni Battista Migliori
Summary: This study aims to compare the number of tuberculosis (TB) cases, drug-resistant TB cases, and TB deaths in 2020 vs 2019 in 11 countries. The results show a decrease in TB cases and drug-resistant TB cases in most countries, except for a few. The TB mortality rate increased in most countries in 2020 compared to 2019. It is important to conduct similar studies in different settings and collect global treatment outcome data for TB/COVID-19 co-infected patients to comprehensively evaluate the medium-term impact of COVID-19 on TB services.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Pharmacology & Pharmacy
Ge Shao, Ziwei Bao, Lina Davies Forsman, Jakob Paues, Jim Werngren, Katarina Niward, Thomas Schon, Judith Bruchfeld, Jan-Willem Alffenaar, Yi Hu
Summary: This study aimed to establish a population pharmacokinetic model for bedaquiline in patients with multidrug-resistant tuberculosis (MDR-TB) in China, and evaluate the currently recommended dosage. The results showed that the recommended dosage of bedaquiline is sufficient for the treatment of MDR-TB, based on the thresholds and minimum inhibitory concentration (MIC) distribution derived from a clinical study.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Samiksha Ghimire, Gladys Molinas, Arturo Battaglia, Nilza Martinez, Luis Gomez Paciello, Sarita Aguirre, Jan-Willem C. Alffenaar, Marieke G. G. Sturkenboom, Cecile Magis-Escurra
Summary: The study aimed to evaluate the difference in drug exposure of rifampicin in native versus non-native Paraguayan populations using dried blood spot samples collected utilizing a limited sampling strategy. The results showed that native patients had comparable drug exposure to non-native patients, with only a small percentage of patients having a rifampicin exposure above the threshold. This study successfully used DBS and limited sampling for the estimation of rifampicin exposure.
Article
Infectious Diseases
K. P. Singh, A. C. C. Carvalho, R. Centis, L. D'Ambrosio, G. B. Migliori, S. G. Mpagama, B. C. Nguyen, R. E. Aarnoutse, A. Aleksa, R. van Altena, P. K. Bhavani, M. S. Bolhuis, S. Borisov, N. van't Boveneind-Vrubleuskaya, J. Bruchfeld, J. A. Caminero, I. Carvalho, J. G. Cho, L. Davies Forsman, M. Dedicoat, K. Dheda, K. Dooley, J. Furin, J. M. Garcia-Garcia, A. Garcia-Prats, A. C. Hesseling, S. K. Heysell, Y. Hu, H. Y. Kim, S. Manga, B. J. Marais, I. Margineanu, A-G. Martson, M. Munoz Torrico, H. M. Nataprawira, E. Nunes, C. W. M. Ong, R. Otto-Knapp, D. J. Palmero, C. A. Peloquin, A. Rendon, D. Rossato Silva, R. Ruslami, A. M. I. Saktiawati, P. Santoso, H. S. Schaaf, B. Seaworth, U. S. H. Simonsson, R. Singla, A. Skrahina, I. Solovic, S. Srivastava, S. L. Stocker, M. G. G. Stukenboom, E. M. Svensson, M. Tadolini, T. A. Thomas, S. Tiberi, J. Trubiano, Z. F. Udwadia, A. R. Verhage, D. H. Vu, O. W. Akkerman, J. W. C. Alffenaar, J. T. Denholm
Summary: This article presents clinical standards for the diagnosis and management of adverse effects (AE) to tuberculosis (TB) treatment. Eight clinical standards were identified, including counseling and evaluation for AE risk, assessment and management of AE, and training for healthcare workers. These standards aim to provide a consensus-based approach to minimize the impact of AE in TB treatment.
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
(2023)
Article
Infectious Diseases
Catriona Halliday, Hannah Yejin Kim, Enoch Tay, Sharon C-A Chen, Jan-Willem Alffenaar
Summary: This study found that statins have synergistic effects with azole antifungal drugs against C. auris isolates, leading to a reduction in the minimum inhibitory concentration of the antifungal drugs. These findings provide a rationale for the combination use of statins and antifungal drugs in the treatment of invasive Candida auris infections.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Sanjay Singh, Tawanda Gumbo, Jan-Willem Alffenaar, Gunavanthi D. Boorgula, Prem Shankar, Tania A. Thomas, Keertan Dheda, Lesibana Malinga, Prithvi Raj, Santosh Aryal, Shashikant Srivastava
Summary: By testing and studying, it was found that the combination of meropenem with beta-lactamase inhibitors (BLIs) could potentially be an effective treatment regimen for multidrug-resistant tuberculosis (MDR-TB). The meropenem-vaborbactam-moxifloxacin backbone regimen has implications for creating a new effective MDR-TB regimen.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)