Article
Microbiology
Akito Kawai, Masahiro Suzuki, Kentaro Tsukamoto, Yusuke Minato, Yohei Doi
Summary: Posttranslational methylation at position A1408 of 16S rRNA leads to pan-aminoglycoside resistance, encompassing both 4,5- and 4,6-disubstituted 2-deoxystreptamine (DOS) aminoglycosides. NpmB1 and NpmB2 are the second group of acquired pan-aminoglycoside resistance 16S rRNA methyltransferases, conferring resistance to all clinically relevant aminoglycosides, including 4,5-DOS agents. The encoding gene for NpmB1 was likely acquired by E. coli from a soil bacterium.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Microbiology
Madhavi L. Kakumanu, Bahjat Fadi Marayati, Ayako Wada-Katsumata, Gideon Wasserberg, Coby Schal, Charles S. Apperson, Loganathan Ponnusamy
Summary: SSI9T strain is a novel bacterium belonging to the genus Sphingobacterium, characterized by distinct phylogenetic, chemotaxonomic, and genomic features.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Pooja Srinivas, Meisam Nosrati, Natalia Zelinskaya, Debayan Dey, Lindsay R. Comstock, Christine M. Dunham, Graeme L. Conn
Summary: Acquired ribosomal RNA (rRNA) methylation is an important mechanism of aminoglycoside resistance in pathogenic bacterial infections. The recognition and modification of a nucleotide in the ribosome decoding center by a specific methyltransferase enzyme has been studied, revealing the critical role of the enzyme's N-terminal domain in recognition and binding to the ribosome.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Microbiology
Soon Dong Lee, Sung-Min Kim, Hong Lim Yang, Yeong-Sik Byeon, In Seop Kim
Summary: A novel Gram stain-negative bacterial strain, named SY4-7(T), was isolated from the rhizosphere mudflat of a halophyte in South Korea. Through 16S rRNA gene sequencing and chemotaxonomic analysis, it was identified as a new species of a new genus in the family Stappiaceae, named Hongsoonwoonella zoysiae sp. nov.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Microbiology
Chuang Han, Mengqi Jiang, Qiqi Shan, Ting Liu, Han Wang, Lifeng Guo, Wensheng Xiang, Junwei Zhao, Xiangjing Wang
Summary: A novel Gram-stain-positive, aerobic actinobacterium, designated Nucisporomicrobium flavum, was isolated from saline-alkali soil collected from Daqing City, Heilongjiang Province, PR China. Genetic and phenotypic analysis showed that this strain is distinct from known genera within the family Micromonosporaceae.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2022)
Article
Microbiology
Rui Yin, Yan-Jun Yi, Zhuo Chen, Guan-Jun Chen, Yan-Xia Zhou, Zong-Jun Du
Summary: A novel Gram-negative bacterium, Flavihalobacter algicola gen. nov., sp. nov., designated Dm15(T), isolated from marine alga in Weihai, China, showed unique characteristics such as growth at a wide range of temperature and salinity, as well as the ability to degrade alginate. It represents a distinct lineage within the family Flavobacteriaceae, with specific genes for cellulase and alginate lyase contributing to its phenotypic characteristics.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Caley A. Hogan, Scott J. Gratz, Jennifer L. Dumouchel, Rajan S. Thakur, Ambar Delgado, Jenna M. Lentini, Kimberly R. Madhwani, Dragony Fu, Kate M. O'Connor-Giles
Summary: TRMT9B is identified as a new regulator of synapse formation and function in Drosophila, specifically enriched in the nervous system. It acts postsynaptically to regulate synaptogenesis and promote neurotransmission, possibly through its methyltransferase function.
Article
Microbiology
Shan Jiang, Feng-Bai Lian, You-Yang Sun, Xiao-Kui Zhang, Zong- Jun Du
Summary: The bacterial strain F7430(T) isolated from coastal sediment in Weihai, PR China, was identified as a novel species within the family Halieaceae, named Sediminihaliea albiluteola gen. nov., sp. nov. The strain exhibited unique physiological and genetic characteristics, distinguishing it as a new species within this family.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Infectious Diseases
Jayalaxmi Wangkheimayum, Deepjyoti Paul, Debadatta Dhar Chanda, K. Melson Singha, Amitabha Bhattacharjee
Summary: This study aimed to analyze the expression of endogenous and exogenous 16S rRNA methyl transferase genes in Escherichia coli under stress of different clinically relevant aminoglycoside antibiotics. The expression analysis showed that the stress of kanamycin, tobramycin, and netilmicin could increase the expression of 16S rRNA methyl transferase genes. Elevated expression of constitutive rsmI can be used as a potential biomarker for detection of acquired aminoglycoside resistance.
INFECTION GENETICS AND EVOLUTION
(2022)
Review
Infectious Diseases
Weiwei Yang, Fupin Hu
Summary: Antimicrobial resistance is a major concern in modern healthcare, with the emergence of 16S-RMTase encoding genes being a significant mechanism for aminoglycoside resistance. The transferable plasmids contribute to the widespread spread of resistance genes among bacteria, posing challenges for the treatment of infectious diseases and infection control.
Article
Public, Environmental & Occupational Health
Saeed Khoshnood, Sousan Akrami, Morteza Saki, Moloudsadat Motahar, Sara Masihzadeh, Sara Daneshfar, Hossein Meghdadi, Effat Abbasi Montazeri, Marjan Abdi, Zahra Farshadzadeh
Summary: This study evaluated the association between aminoglycoside resistance, virulence genes, and biofilm formation capacity in K. pneumoniae isolated from hospitalized patients in South-West of Iran. The results showed that K. pneumoniae strains had the highest resistance to tobramycin and the lowest resistance to amikacin. Majority of the strains were biofilm producers and there was a significant association between antibiotic resistance pattern and the strength of biofilm production.
HEALTH SCIENCE REPORTS
(2023)
Article
Microbiology
Javier E. Fernandez, Helena M. B. Seth-Smith, Patrice Nordmann, Adrian Egli, Andrea Endimiani, Vincent Perreten
Summary: This study discovered a novel multidrug resistance plasmid pJEF1-OXA-181, which carries carbapenemase and aminoglycoside resistance genes. It highlights the risk of multidrug resistance plasmids spreading in the healthcare system and calls for continuous monitoring of multidrug-resistant bacteria in high-risk patients.
MICROBIOLOGY SPECTRUM
(2022)
Article
Microbiology
Maropeng C. Monyama, Oriel M. Taioe, Jane S. Nkhebenyane, Deidre van Wyk, Tsepo Ramatla, Oriel M. M. Thekisoe
Summary: This study collected houseflies from hospices and used genetic sequencing to detect the diversity of bacteria and antibiotic resistance genes they carried. The results showed that houseflies carried various potentially pathogenic bacteria and antibiotic resistance genes. This indicates a potential health risk to patients in hospices and the surrounding community, highlighting the importance of strict control of houseflies and maintaining high standards of hygiene and food safety.
Article
Microbiology
Ashish Verma, Shiva S. Sundharam, Yash Pal, Bhawana Bisht, Pooja Yadav, Srinivasan Krishnamurthi
Summary: SAOS 153DT, a novel Gram-stain-negative, rod-shaped bacterium, shares 99.4% sequence identity with Yangia pacifica DX5-10T. The chemotaxonomic and genomic characteristics support its designation as a novel species within the genus Yangia.
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
(2021)
Article
Food Science & Technology
Meera Ongmu Bhutia, Namrata Thapa, Jyoti Prakash Tamang
Summary: The microbiological quality of traditionally preserved fish products from Sikkim, India was assessed in this study, revealing the presence of various potential pathogenic bacteria. Antibiotic susceptibility testing showed that the majority of samples were sensitive to several antibiotics. However, enterotoxins produced by Bacillus cereus and Staphylococcus were detected in some samples, highlighting potential health risks associated with these products.
