Article
Chemistry, Multidisciplinary
Jiahong Chen, Jianwei Zhong, Yifu Chang, Yinning Zhou, Seok Hwee Koo, Thean Yen Tan, Hongtao Lei, Ye Ai
Summary: Antimicrobial resistance is a serious threat to global public health. A label-free electrical impedance-based microfluidic platform has been designed to expedite and streamline antimicrobial susceptibility testing (AST) for clinical practice, with a rapid 2-minute AST assay at the single-bacterium level. This platform enables accurate analysis of bacterial viability and determination of antimicrobial resistance.
Article
Biophysics
Pragya Swami, Ayush Sharma, Satyam Anand, Shalini Gupta
Summary: Antimicrobial resistance (AMR) is caused by inappropriate or excessive antibiotic consumption. Early diagnosis of bacterial infections can greatly curb empirical treatment and thus AMR. The authors introduce a real-time, label-free approach called DEPIS that combines dielectrophoresis for bacterial enrichment and impedance spectroscopy for cell viability analysis in under 60 minutes. This rapid cell analyses approach can aid in faster diagnosis of bacterial infections and benefit the clinical decision-making process for antibiotic treatment, addressing the critical issue of AMR.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Naoki Uno, Ziyue Li, Lori Avery, Maroun M. Sfeir, Changchun Liu
Summary: CRISPR technology combined with isothermal amplification has been used as a powerful biosensing tool for sensitive nucleic acid detection. However, integrating isothermal amplification into CRISPR detection in a one-pot system has been challenging. In this study, a simple CRISPR gel biosensing platform was developed for HIV RNA detection, which successfully detected down to 30 copies of HIV RNA per test within 30 minutes.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Dan Li, Xiao-Long Zhang, Ya-Qin Chai, Ruo Yuan
Summary: A high-efficiency controllable three-dimensional DNA nanomachine (CDNM) was developed by regulating the core diameter and DNAzyme cantilever length, which exhibited greater amplification efficiency and faster walking rate compared to traditional 3D DNA nanomachines. The CDNM could be activated by enzyme-free-target-recycling amplification (EFTRA) to achieve fast cleavage and walking on the biosensor surface under the interaction of Mg2+ when the target miRNA-21 was present. The designed electrochemical biosensor successfully detected target miRNA-21 at an ultrasensitive level with a wide response range and low detection limit.
ANALYTICAL CHEMISTRY
(2023)
Article
Biochemical Research Methods
Mohammad Osaid, Yi-Sin Chen, Chih-Hung Wang, Anirban Sinha, Wen-Bin Lee, Priya Gopinathan, Hung-Bin Wu, Gwo-Bin Lee
Summary: The study developed a microfluidic platform for rapid antimicrobial susceptibility testing, including dilution of antibiotics and distribution of bacteria, which can improve efficiency and accuracy of the testing process.
Article
Biophysics
Andrew Piper, Ingrid Oberg Mansson, Shirin Khaliliazar, Roman Landin, Mahiar Max Hamedi
Summary: Wearable sensors have the potential to revolutionize biomarker detection, with the ability to produce cheap and disposable sensors that can be easily functionalized for a broad range of biomarkers. These sensors are stretchable and have shown promising results in detecting glucose in human sweat, making them ideal for point-of-care sensing applications. The sensors have a high sensitivity and low detection limit, demonstrating their potential for biomarker detection.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biophysics
Lik-Voon Kiew, Chia-Yu Chang, Sheng-Yu Huang, Pei-Wen Wang, Choon-Han Heh, Chung-Te Liu, Chia-Hsin Cheng, Yi-Xiang Lu, Yen-Chen Chen, Yi-Xuan Huang, Sheng-Yun Chang, Huei-Yu Tsai, Yu-An Kung, Peng-Nien Huang, Ming-Hua Hsu, Bey-Fen Leo, Yiing-Yee Foo, Chien-Hao Su, Kuo-Chen Hsu, Po-Hsun Huang, Chirk-Jenn Ng, Adeeba Kamarulzaman, Chiun-Jye Yuan, Dar-Bin Shieh, Shin-Ru Shih, Lip-Yong Chung, Chia-Ching Chang
Summary: The study developed a biosensing platform for the rapid screening of potential inhibitors against the binding of S-protein and ACE2. Several compounds were identified to reduce the binding affinity of S-protein to ACE2, thereby decreasing the infectivity of SARS-CoV-2 to human oral cavity squamous carcinoma cells expressing ACE2. This highlights the utility of the developed biosensing platform in identifying modulators for S-protein-ACE2 binding.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Elif Burcu Aydin, Muhammet Aydin, Mustafa Kemal Sezginturk
Summary: In this study, an electrochemical biosensor modified with gold nanoparticles and amino-substituted-pyrrole polymer (AuNPs/P(PyAmn)) was developed for label-free detection of CYFRA 21-1, an important lung cancer biomarker. The biosensor was fabricated by electropolymerization of PyAmn on an ITO platform, followed by electrodeposition of AuNPs and binding of an anti-CYFRA 21-1 recognition element. The biosensor exhibited high sensitivity, stability, and specificity for CYFRA 21-1 detection, and could be successfully applied to real serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Infectious Diseases
Felix Roth, Nathan D. Leedahl, David D. Leedahl, Dubert M. Guerrero
Summary: The rapid identification of pathogens causing bloodstream infections is crucial in clinical microbiology laboratories. While most methods have reduced turnaround time and impact on clinical decisions, they do not provide complete antimicrobial susceptibility testing (AST) information. By employing rapid identification (ID) and AST with the Accelerate PhenoTest, the study observed a significant decrease in hospital stay, reduction in broad-spectrum antibiotic use, and potential cost savings.
Article
Chemistry, Physical
Said M. El-Sheikh, Diaa Osman, Omnia Ali, Wafaa Gh Shousha, Madiha A. Shoeib, Sherif M. Shawky, Sheta M. Sheta
Summary: The study developed a novel label-free electrochemical biosensor for direct detection of unamplified HCV nucleic acid based on bimetallic metal-organic framework (Ag/Zn-MOF). The biosensor showed high sensitivity, low detection limit, wide detection range, low cost, and ease of use. The bimetallic MOF exhibited excellent catalytic activity for electrochemical signal amplification.
APPLIED SURFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Daniel Spencer, Yuetao Li, Yiling Zhu, J. Mark Sutton, Hywel Morgan
Summary: A novel test has been developed to assess the susceptibility of bacteria to antibiotics by measuring changes in impedance, providing a rapid determination of electrical minimum inhibitory concentration (eMIC). This test accurately predicts sensitivity or resistance of bacterial strains to various antibiotics within a fraction of the time compared to traditional methods.
Article
Chemistry, Multidisciplinary
Morteza Azizi, Benyamin Davaji, Ann Nguyen, Shiying Zhang, Belgin Dogan, Kenneth W. Simpson, Alireza Abbaspourrad
Summary: This study proposed a novel gradient-based microchamber microfluidic platform for rapid antimicrobial susceptibility testing, which can cover a wide range of antibiotic concentrations in a single test with a short time to results. The platform demonstrated good tunability and applicability for profiling different bacteria, and tracked bacterial growth independently of antibiotic mechanism or bacterial species in a robust and easy-to-implement manner.
Article
Chemistry, Analytical
Xiaoqian Wei, Shaojia Song, Weiyu Song, Weiqing Xu, Lei Jiao, Xin Luo, Nannan Wu, Hongye Yan, Xiaosi Wang, Wenling Gu, Lirong Zheng, Chengzhou Zhu
Summary: The rational construction of advanced sensing platforms to sensitively detect H2O2 produced by living cells is a challenge in both physiological and pathological fields. Single atomic site catalysts (SASCs) with intrinsic peroxidase (POD)-like activity have shown great promise for H2O2 detection, but there is still a gap between them and natural enzymes. A new design of synergistically enhanced single atomic sites for electrochemical sensing applications is proposed in this work.
ANALYTICAL CHEMISTRY
(2021)
Review
Medicine, General & Internal
Ritu Banerjee, Romney Humphries
Summary: Antimicrobial susceptibility testing of bacteria isolated in blood cultures is crucial for managing patients with sepsis. This review discusses new methods for AST and their potential impact on clinical outcomes of patients with bloodstream infections.
FRONTIERS IN MEDICINE
(2021)
Article
Chemistry, Analytical
Weifeng Zhang, Yun Li, Lin Yao, Fan Wu, Chen Chen, Liqun Zhou, Bo Zheng, Pu Wang, Weili Hong
Summary: A new method based on SRS microscopy was reported to rapidly determine the antimicrobial susceptibility of beta-lactam antibiotics by measuring both the metabolic activity and morphological deformation of bacteria. The evaluation method achieved a 93.2% categorical agreement and a 93.2% essential agreement with the standard reference method when performing AST of cefotaxime on 103 E. coli strains.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Ludmila Moranova, Michal Stanik, Roman Hrstka, Susana Campuzano, Martin Bartosik
Summary: A novel method for detecting prostate cancer by combining electrochemical assay with LAMP technology has been developed, allowing for direct detection of two RNA biomarkers PCA3 and PSA in urine samples. This method demonstrates good sensitivity and specificity, providing a minimally invasive diagnostic format.
Article
Chemistry, Analytical
Beatriz Arevalo, Marina Blazquez-Garcia, Alejandro Valverde, Veronica Serafin, Ana Montero-Calle, Guillermo Solis-Fernandez, Rodrigo Barderas, Susana Campuzano, Paloma Yanez-Sedeno, Jose M. Pingarron
Summary: A dual immunosensor was developed for the simultaneous determination of two important immunity-related cytokines, BAFF and APRIL. The immunosensor showed high sensitivity and low detection limits, making it suitable for detecting these cytokines in cancer cell lysates and serum samples.
Article
Chemistry, Analytical
Marina Blazquez-Garcia, Beatriz Arevalo, Veronica Seraffin, Sara Benede, Luis Mata, Patricia Galan-Malo, Isabel Segura-Gil, Maria Dolores Perez, Jose M. Pingarron, Susana Campuzano
Summary: This study developed the first electrochemical bioplatform for detecting soy traces in food. The bioplatform utilizes specific antibodies and magnetic microbeads to perform sandwich-type immunoassays. The electrochemical detection method showed good selectivity and sensitivity, with a short detection time and accurate results comparable to ELISA methodologies.
Article
Materials Science, Multidisciplinary
Susana Campuzano, Maria Pedrero, Rodrigo Barderas, Jose M. Pingarron
Summary: Electrochemical biosensors are rapidly advancing in terms of sensitivity, selectivity, simplicity, response time, and antifouling ability, thanks to the design and development of nanostructured or multifunctional nucleic acid or peptide biomaterials. This perspective article critically discusses and overviews the recent progress in electrochemical biosensors involving these biomaterials, providing insights into potential and opportunities. The challenges and future directions are also highlighted.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Chemistry, Analytical
Goksu Ozcelikay, Maria Gamella, Mehmet Altay Unal, Kivilcim Gucuyener, Ana Montero-Calle, Rodrigo Barderas, Jose M. Pingarron, Susana Campuzano, Sibel A. Ozkan
Summary: This study presents a magnetic microbeads-based electrochemical immunoplatform for the detection of GFAP, which has potential clinical applications in the diagnosis and prognosis of neurodegenerative diseases. The platform demonstrates low detection limit, good selectivity, and the ability to differentiate between healthy individuals and patients with VD and AD.
Review
Chemistry, Analytical
Esther Sanchez-Tirado, Lourdes Agui, Araceli Gonzalez-Cortes, Susana Campuzano, Paloma Yanez-Sedeno, Jose Manuel Pingarron
Summary: The study of the human microbiome is a multidisciplinary field that holds great potential for improving disease diagnosis and monitoring using microbiota biomarkers. Electrochemical biosensors, with their high sensitivity and fast response, combined with advancements in nanomaterials, offer unique tools for understanding the human microbiome and driving future developments. This review article focuses on the application of electrochemical (bio)sensors for monitoring microbial metabolites and disease biomarkers in the gastrointestinal microbiome, discussing challenges and future directions.
Article
Chemistry, Analytical
Beatriz Arevalo, Veronica Serafin, Maria Garranzo-Asensio, Ana Montero-Calle, Rodrigo Barderas, Paloma Yanez-Sedeno, Susana Campuzano, Jose M. Pingarron
Summary: This study presents the first amperometric biosensor capable of simultaneously determining the single or total content of various human immunoglobulin isotypes of anti-dsDNA antibodies, which are important biomarkers in autoimmune and neurodegenerative diseases. The biosensor involves the use of functionalized magnetic microparticles and specific secondary antibodies for detection. Clinical applications of the biosensor were successful in determining hIgs classes in healthy individuals, as well as patients with SLE and AD, yielding results consistent with established detection methods.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Victor Perez-Gines, Rebeca M. Torrente-Rodriguez, Maria Pedrero, Neus Martinez-Bosch, Pablo Garcia de Frutos, Pilar Navarro, Jose M. Pingarron, Susana Campuzano
Summary: Pancreatic ductal adenocarcinoma (PDAC) is the third leading cause of cancer-related deaths in developed countries and predicted to be the second leading cause in 2030. A disposable amperometric immunoplatform for the determination of CA19-9 has been developed in this study. The immunoplatform combines magnetic microsupports (MBs) for immunoassay implementation and amperometric transduction on screen-printed carbon electrodes (SPCEs). The immunoplatform showed good reproducibility, selectivity, and storage stability, and was able to differentiate healthy subjects from PDAC patients within 1 hour. It represents an improvement in terms of cost, applicability, and accessibility compared to current ELISA-based techniques.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Susana Campuzano, Maria Gamella, Maria Pedrero, Jose M. Pingarron
Summary: The role of liquid biopsy in precision, equitable, and sustainable diagnosis of prevalent and highly heterogeneous diseases using biomarkers derived from whole cells, secretomes, and exosomes is undeniable. Electrochemical biosensing and biosensors, particularly those involving affinity reactions, have emerged as competitive and cost-effective analytical alternatives for identifying these challenging targets. This review provides a concise and up-to-date overview of the fundamentals, versatility, and opportunities offered by state-of-the-art electrochemical biosensing (2018-2022) for the determination of cellular-derived biomarkers. It also offers critical insights into the short-term challenges and future research directions in this field.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Oncology
Ana Montero-Calle, Maria Garranzo-Asensio, Rebeca M. Torrente-Rodriguez, Victor Ruiz-Valdepenas Montiel, Carmen Poves, Jana Dziakova, Rodrigo Sanz, Cristina Diaz del Arco, Jose Manuel Pingarron, Maria Jesus Fernandez-Acenero, Susana Campuzano, Rodrigo Barderas
Summary: The study aimed to assess whether p53 and p63 proteoforms derived from alternative splicing could have a differential seroreactivity and higher diagnostic value than canonical proteins in colorectal cancer. The researchers demonstrated the appearance of a differential seroreactivity among the proteoforms in colorectal cancer patients. These findings reveal increased complexity of the humoral immune response in cancer and highlight the potential for developing a blood-based biomarker signature for colorectal cancer detection.
Review
Biochemical Research Methods
Susana Campuzano, Rodrigo Barderas, Maria Teresa Moreno-Casbas, Angeles Almeida, Jose M. Pingarron
Summary: In the era of personalization, personalized management of medicine and nutrition is crucial for life expectancy and quality of life. Implementing precision medicine and nutrition is a complex challenge that requires new technologies to meet cost, simplicity, and versatility requirements, as well as accurately determining molecular markers in biofluids at different omics levels almost in real time. This review critically discusses recent advances and positions electrochemical bioplatforms as suitable tools for advanced diagnostics, therapy, and precision nutrition.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Ponnusamy Nandhakumar, Cristina Munoz San Martin, Beatriz Arevalo, Shichao Ding, Mahika Lunker, Eva Vargas, Omeed Djassemi, Susana Campuzano, Joseph Wang
Summary: In order to address the low sensitivity issue of conventional LFA, we report a simple electrochemical LFA with nanocatalytic redox cycling for decentralized insulin detection. This method achieves higher sensitivity than traditional methods by amplifying the signal through nanoparticles and chemical reactions.
Review
Chemistry, Multidisciplinary
Susana Campuzano, Jose M. Pingarron
Summary: Electrochemical affinity biosensors are rapidly evolving and becoming increasingly important in our daily lives. They combine their intrinsic attributes with advances in technology to offer a wide range of opportunities and applications. This perspective provides an updated and critical view of these biosensors and discusses the challenges and future directions they face.
Review
Chemistry, Physical
Susana Campuzano, Jose M. Pingarron
Summary: The recent advances in electrochemical biosensing have demonstrated the versatility and adaptability of biotools. Innovations in bioreceptor production, electrode substrate fabrication, miniaturizable electronics, materials science, digitization, and artificial intelligence have also contributed to the evolution of electrochemical biosensing.
CURRENT OPINION IN ELECTROCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Sandra Tejerina-Miranda, Maria Pedrero, Marina Blazquez-Garcia, Veronica Serafin, Ana Montero-Calle, Maria Garranzo-Asensio, A. Julio Reviejo, Jose M. Pingarron, Rodrigo Barderas, Susana Campuzano
Summary: This study developed the first electrochemical bioplatform for determining human endostatin, which shows high sensitivity and selectivity for diagnosing tumors and identifying patients in different stages.
BIOELECTROCHEMISTRY
(2024)