Article
Multidisciplinary Sciences
Lucie Semenec, Amy K. K. Cain, Catherine J. J. Dawson, Qi Liu, Hue Dinh, Hannah Lott, Anahit Penesyan, Ram Maharjan, Francesca L. L. Short, Karl A. A. Hassan, Ian T. T. Paulsen
Summary: This study characterizes the genomes of A. baumannii and K. pneumoniae strains co-isolated from a single human lung infection, and investigates their interactions through various assays. The findings shed light on their effects on antimicrobial resistance and virulence during co-infection.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Anthony Sophonsri, Corey Kelsom, Mimi Lou, Paul Nieberg, Annie Wong-Beringer
Summary: The study found that patients coinfected with CRKP and another carbapenem-resistant pathogen have more significant morbidity and require longer hospital stays compared to those with CRKP monoinfection. Empiric therapy targeting both CRKP and carbapenem-resistant Pseudomonas aeruginosa may be beneficial for at-risk patients with pneumonia.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Ruohan Wei, Xuemei Yang, Han Liu, Tongyao Wei, Sheng Chen, Xuechen Li
Summary: The study demonstrates that chemically synthesized Pse-CRM197 conjugates can stimulate high immune responses and provide a new alternative for controlling infections caused by multidrug-resistant A. baumannii.
ACS CENTRAL SCIENCE
(2021)
Article
Biology
Jaeeun Park, Misung Kim, Bora Shin, Mingyeong Kang, Jihye Yang, Tae Kwon Lee, Woojun Park
Summary: The modification of outer membrane charge induced by polymyxin B (PMB) in Acinetobacter baumannii leads to PMB-resistant variants and clinical isolates producing outer membrane vesicles (OMVs). Upregulation of the pmr operon and decreased membrane-linkage proteins result in enhanced biofilm formation and protection of susceptible bacteria from PMB, suggesting OMVs function as decoys against PMB. OMVs also protect the microbial community against PMB treatment in anaerobic conditions, indicating their potential clinical significance in antibiotic resistance.
Article
Multidisciplinary Sciences
Sachio Suzuki, Phawinee Subsomwong, Kouji Narita, Noriaki Kawai, Takahito Ishiai, Wei Teng, Rojana Sukchawalit, Akio Nakane, Sadatomo Tasaka, Krisana Asano
Summary: By comparing AbOMVs isolated under hypoxic and normoxic conditions, it was found that they have different protein profiles. While both AbOMVs had similar cytotoxic effects on bacteria, AbOMVs from normoxia induced higher levels of the cytokine TNF-alpha production and invasion of Staphylococcus aureus and Pseudomonas aeruginosa. On the other hand, AbOMVs from hypoxia significantly enhanced A. baumannii invasion into lung epithelial cells. This finding sheds light on the complex interactions between A. baumannii, coinfecting pathogens, and host cells mediated by OMVs, particularly the different pathogenic effects of AbOMVs under normoxic and hypoxic conditions.
Article
Immunology
Rushuang Yan, Ye Lu, Yiwei Zhu, Peng Lan, Shengnan Jiang, Jun Lu, Ping Shen, Yunsong Yu, Jiancang Zhou, Yan Jiang
Summary: The study characterized a carbapenem-resistant hypervirulent K. pneumoniae isolate from Zhejiang, China, demonstrating resistance to multiple antibiotics and harboring a bla (KPC-2) carrying plasmid. Transfer of the plasmid to P. aeruginosa was shown to confer carbapenem resistance, posing a serious threat to public health.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Irfan Ahmad, Aftab Nadeem, Fizza Mushtaq, Nikola Zlatkov, Muhammad Shahzad, Anton V. Zavialov, Sun Nyunt Wai, Bernt Eric Uhlin
Summary: Acinetobacter baumannii is a common drug-resistant bacterial pathogen, and its biofilms and pili play important roles in antibiotic resistance and adherence to epithelial cells.
NPJ BIOFILMS AND MICROBIOMES
(2023)
Article
Medical Laboratory Technology
Pengwen Ouyang, Bin Jiang, Na Peng, Juan Wang, Liang Cai, Yi Wu, Jianrong Ye, Yiping Chen, Hao Yuan, Chaochao Tan, Liming Tan, Liangyi Xie
Summary: The study analyzed the microbiological and clinical characteristics of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKP) that causes nosocomial infection. The main type of CRKP that caused nosocomial infection was found to be ST11 KPC-2-producing CR-hvKP, and clonal spread has occurred.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Infectious Diseases
Lei Zha, Xue Zhang, Yusheng Cheng, Qiancheng Xu, Lingxi Liu, Simin Chen, Zhiwei Lu, Jun Guo, Boris Tefsen
Summary: The benefit of combining polymyxin and tigecycline for treating carbapenem-resistant bacterial infections is uncertain. A cohort study found that adding polymyxin B to high-dose tigecycline therapy did not lead to better clinical outcomes in pneumonia patients caused by carbapenem-resistant Klebsiella pneumoniae and Acinetobacter baumannii. The study recommended against adding polymyxin B to the high-dose tigecycline regimen for this type of pneumonia.
Article
Infectious Diseases
Constantinos Tsioutis, Vanessa M. Eichel, Nico T. Mutters
Summary: This critical review aimed to evaluate the effectiveness of measures to control the spread of KPC-KP in healthcare settings, finding that successful control during outbreaks doesn't necessarily indicate the effectiveness of individual infection control measures, due to low-quality evidence and lack of controlled intervention studies. The distribution of studies, reported cases, and high mortality rates highlight that KPC-KP remains a major healthcare problem globally.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Microbiology
Bosul Lee, Jun Yan, Amber Ulhaq, Sarah Miller, Wonjae Seo, Peggy Lu, Rosemary She, Brad Spellberg, Brian Luna
Summary: The antimicrobial activity of rifabutin against Acinetobacter baumannii is best modeled by using RPMI for in vitro testing. The hypersusceptibility of A. baumannii to rifabutin was observed both in vitro and in vivo using a Galleria mellonella infection model. Resistance emergence frequency was higher in vitro when bacteria were selected on RPMI compared to TSA or MHII plates, but lower in vivo compared to the RPMI in vitro condition.
Article
Multidisciplinary Sciences
Woo Cheol Lee, Sungjae Choi, Ahjin Jang, Kkabi Son, Yangmee Kim
Summary: The study focused on the crystal structures of the beta-ketoacyl-ACP reductase ApeQ produced by an APE gene cluster in Gram-negative bacteria. It was found that ApeQ plays a crucial role in APE biosynthesis and cannot be substituted by a similar enzyme from the FAS gene cluster. Structural and functional analysis revealed a positively charged arginine patch in ApeQ as the cognate ACP-binding site.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Jingchun Kong, Yue Wang, Zhuocheng Yao, Yishuai Lin, Yi Zhang, Yijia Han, Tieli Zhou, Jianzhong Ye, Jianming Cao
Summary: In this study, the combination of colistin and eugenol was found to have a significant synergistic antibacterial effect against colistin-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae. The combination increased membrane permeability and caused membrane damage, inhibiting bacteria synergistically. Eugenol was non-toxic to cells and the combination was effective in vivo. These findings suggest that the colistin/eugenol combination may be a viable treatment option for colistin-resistant bacterial infections.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Jingchun Kong, Yue Wang, Zhuocheng Yao, Yishuai Lin, Yi Zhang, Yijia Han, Tieli Zhou, Jianzhong Ye, Jianming Cao
Summary: This study found that the combination of colistin and eugenol has a significant synergistic effect in treating colistin-resistant Pseudomonas aeruginosa and Klebsiella pneumoniae infections, reversing their sensitivity to colistin. Eugenol enhances membrane permeability and causes membrane damage, leading to inhibition of bacteria. The combination of colistin and eugenol is also effective in vivo. These findings suggest that the colistin/eugenol combination may be a viable treatment option for clinical infections caused by colistin-resistant pathogens.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Qing Yang, Yue Li, Li Fang, Tailong Lei, Heng Cai, Xiaoting Hua, Min Zheng, Yunsong Yu
Summary: This study characterized a novel KPC-113 variant in a clinical Pseudomonas aeruginosa isolate R20-14. Genomic analysis revealed a plasmid carrying blaKPC-113, which conferred both carbapenem and ceftazidime-avibactam resistance. KPC-113 presented reduced catalytic ability and poor response to avibactam inhibition compared to KPC-2. Efforts should be made to prevent the horizontal spread of blaKPC-113 in clinical settings.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Microbiology
Iraida E. Robledo, Edna E. Aquino, Maria I. Sante, Jorge L. Santana, Diana M. Otero, Carlos F. Leon, Guillermo J. Vazquez
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2010)
Article
Microbiology
Daniel J. Wolter, Noha Khalaf, Iraida E. Robledo, Guillermo J. Vazquez, Maria I. Sante, Edna E. Aquino, Richard V. Goering, Nancy D. Hanson
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2009)
Article
Microbiology
T. Martinez, G. J. Vazquez, E. E. Aquino, R. V. Goering, I. E. Robledo
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2012)
Letter
Infectious Diseases
Teresa Martinez, Guillermo J. Vazquez, Edna E. Aquino, Ramon Ramirez-Ronda, Iraida E. Robledo
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2012)
Article
Microbiology
Teresa Martinez, Guillermo J. Vazquez, Edna E. Aquino, Idali Martinez, Iraida E. Robledo
JOURNAL OF MEDICAL MICROBIOLOGY
(2014)
Article
Cell Biology
RV Flores, MG Hernández-Pérez, E Aquino, RC Garrad, GA Weisman, FA Gonzalez
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2005)
Article
Developmental Biology
WI Silva, HM Maldonado, G Velázquez, M Rubio-Dávila, JD Miranda, E Aquino, N Mayol, A Cruz-Torres, J Jardón, IK Salgado-Villanueva
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE
(2005)
Article
Infectious Diseases
E Aquino-Piñero, N Rodríguez-Del Valle
