Article
Microbiology
Alexandra Irrgang, Ge Zhao, Katharina Juraschek, Annemarie Kaesbohrer, Jens A. Hammerl
Summary: The study found widespread distribution of the SHV-12 variant in German poultry production, with dissemination primarily driven by horizontal gene transfer. The isolates carrying bla(SHV-12) gene were shown to have low phylogenetic relationships, and the gene was located on transferable plasmids in most cases. Consumers are at risk when handling raw poultry meat and should pay attention to proper kitchen hygiene practices.
Article
Multidisciplinary Sciences
Assia Zemmour, Radia Dali-Yahia, Makaoui Maatallah, Nadjia Saidi-Ouahrani, Bouabdallah Rahmani, Nora Benhamouche, Hissa M. Al-Farsi, Christian G. Giske
Summary: This study characterized the resistome, virulome, mobilome, and CRISPR-Cas system of ESBL-KP isolates in Algeria and found them to be polyclonal. The discovery of qnrS in ESBL-KP in Algeria was reported for the first time. The co-existence of virulence and resistance genes highlights the importance of genomic surveillance in hospital settings.
Article
Microbiology
Razib Mazumder, Arif Hussain, Ahmed Abdullah, Md Nazrul Islam, Md Tuhin Sadique, S. M. Muniruzzaman, Anika Tabassum, Farhana Halim, Nasrin Akter, Dilruba Ahmed, Dinesh Mondal
Summary: This study reveals a high diversity of E. coli lineages among ESBL-producing E. coli from Dhaka, with ongoing circulation of ST131 and other major non-ST131 high-risk clones that are strongly associated with cephalosporin resistance and virulence genes, highlighting the need for prospective monitoring of high-risk clones to prevent worsening of the AMR crises.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Samiratu Mahazu, Wakana Sato, Alafate Ayibieke, Isaac Prah, Takaya Hayashi, Toshihiko Suzuki, Shiroh Iwanaga, Anthony Ablordey, Ryoichi Saito
Summary: The emergence and dissemination of ESBL-producing bacteria, particularly Enterobacteriaceae, have posed serious challenges to healthcare in Ghana. This study investigated the antimicrobial susceptibility and genetic characteristics of 164 Escherichia coli strains isolated from infected patients in two hospitals. It was found that 62.2% of the cefotaxime-resistant isolates were ESBL-producers, with ST131 being the dominant group. The ESBL-producers carried different ESBL alleles, with bla(CTX-M-15) being the most predominant. This study provides the first report on the phylogeny of ST131 in Ghana.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Mabel Kamweli Aworh, Eme Ekeng, Pernille Nilsson, Beverly Egyir, Christian Owusu-Nyantakyi, Rene S. Hendriksen
Summary: This study investigated the zoonotic transmission of extended-spectrum beta-lactamase-producing E. coli (ESBL-EC) among humans, beef cattle, and abattoir environments in Nigeria. The study found a high prevalence of ESBL-EC in beef cattle and abattoir workers, highlighting the importance of genomic surveillance and raising awareness among healthcare workers.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Mitul A. Patel, Aparna Pandey, A. C. Patel, S. S. Patel, H. C. Chauhan, M. D. Shrimali, Pankaj A. Patel, S. K. Mohapatra, B. S. Chandel
Summary: This study revealed the molecular characteristics and high occurrence of ESBL E. coli in poultry in Gujarat, India, mainly attributed to the genes bla(TEM), bla(SHV), and bla(OXA).
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Marita Vedovelli Cardozo, Apostolos Liakopoulos, Michael Brouwer, Arie Kant, Lucas Jose Luduverio Pizauro, Mariana Monezi Borzi, Dik Mevius, Fernando Antonio de Avila
Summary: This study in Sao Paolo, Brazil, investigated the phylogenetic diversity and epidemiology of ESBL-producing E. coli and K. pneumoniae from chicken, chicken meat, and human clinical isolates. The ESBL genes were characterized and their location determined on plasmids. The study found genetic diversity in isolates from chickens, chicken meat, and human feces, suggesting possible transmission of ESBL-producing pathogens from chickens to humans.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Infectious Diseases
Maria Giufre, Elena Mazzolini, Marina Cerquetti, Silvio Brusaferro
Summary: This study compared ESBL-producing Escherichia coli isolates from humans and food-producing animals, finding different types of ESBLs in different sources. The results suggest the potential exchange of ESBL genes from animals to humans, emphasizing the need for strict monitoring based on a 'One Health' approach.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Food Science & Technology
Sara A. Burgess, Jacinda Aplin, Patrick J. Biggs, Georgia Breckell, Jackie Benschop, Ahmed Fayaz, Leah J. Toombs-Ruane, Anne C. Midwinter
Summary: This study found a low prevalence of ESBL and pACBL producing E. coli on pasture-based farms, with only three farms testing positive for ESBL- or ACBL-producing strains.
INTERNATIONAL DAIRY JOURNAL
(2021)
Article
Immunology
Lisandra Aguilar-Bultet, Claudia Bagutti, Adrian Egli, Monica Alt, Laura Maurer Pekerman, Ruth Schindler, Reto Furger, Lucas Eichenberger, Tim Roloff, Ingrid Steffen, Philipp Huebner, Tanja Stadler, Sarah Tschudin-Sutter
Summary: This report describes a cluster of extended-spectrum beta-lactamase (ESBL)-producing Klebsiella pneumoniae sequence type 101, derived from 1 poultry and 2 clinical samples collected within the setting of a prospective study designed to determine the diversity and migration of ESBL-producing Enterobacterales between humans, foodstuffs, and wastewater.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Microbiology
Ralf Lopes, Danny Fuentes-Castillo, Herrison Fontana, Larissa Rodrigues, Karine Dantas, Louise Cerdeira, Isabel Henriques, Nilton Lincopan
Summary: The study identified high-risk clones of ESBL-producing E. coli and K. pneumoniae in fresh vegetables sold in a high ESBL endemic country, showing resistance to clinically relevant antibiotics. These endophytic isolates have the ability to colonize plant tissues and demonstrate a multidrug-resistant profile.
Article
Infectious Diseases
Se Ra Shin, Seong Mi Noh, Woo Kyung Jung, Sook Shin, Young Kyung Park, Dong Chan Moon, Suk-Kyung Lim, Yong Ho Park, Kun Taek Park
Summary: The study conducted in South Korea between 2017 and 2019 identified various ESC-resistant bacterial isolates in companion animals, with predominant species being E. coli, Klebsiella, and others. These isolates displayed diversity in sequence types and resistance genes.
Article
Multidisciplinary Sciences
Mayo Yasugi, Shingo Hatoya, Daisuke Motooka, Yuki Matsumoto, Shunsuke Shimamura, Hiroyuki Tani, Masaru Furuya, Keiichiro Mie, Masami Miyake, Shota Nakamura, Terumasa Shimada
Summary: This study suggests that companion dogs can spread ESBL/AmpC-producing ExPEC through their feces, with some strains and plasmids potentially being transmitted between humans and companion dogs. Therefore, companion dogs may serve as an important reservoir for ESBL/AmpC-producing E. coli in the community.
Article
Agriculture, Dairy & Animal Science
Heba Badr, Reem M. Reda, Naglaa M. Hagag, Essam Kamel, Sara M. Elnomrosy, Amal I. Mansour, Momtaz A. Shahein, Samah F. Ali, Hala R. Ali
Summary: The study reveals the emergence of resistant bugs such as ESBL-E. coli in Egypt due to sub-therapeutic doses given to food chain animals. Infections caused by these superbugs are linked to high morbidity/mortality rates. The study suggests a potential zoonotic transmission between human and chicken isolates, emphasizing the importance of managing antibiotic use in veterinary field.
Review
Immunology
Hiroki Namikawa, Waki Imoto, Koichi Yamada, Yoshihiro Tochino, Yukihiro Kaneko, Hiroshi Kakeya, Taichi Shuto
Summary: This systematic review and meta-analysis identified predictors of mortality from extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-PE) bacteremia. Previous antimicrobial therapy, neutropenia, nosocomial infection, rapidly fatal underlying disease, respiratory tract infection, Pitt bacteremia score, severe sepsis, and urinary tract infection were found to be predictors of mortality. Appropriate empirical therapy was identified as a protective factor against mortality.
EMERGING MICROBES & INFECTIONS
(2023)
Article
Infectious Diseases
Andrea Garcia-Caballero, Juan de Dios Caballero, Ainhize Maruri, Maria Isabel Serrano-Tomas, Rosa del Campo, Maria Isabel Morosini, Rafael Canton
Summary: FOX DD and MicroScan-WalkAway (R) show high sensitivity and specificity for MRSA detection among CF isolates, with ChromIDTM-MRSA having excellent sensitivity but poorer specificity for CF isolates. The study suggests using FOX DD and MicroScan-WalkAway (R) to confirm ChromIDTM-MRSA positive CF cultures.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Review
Microbiology
Christian G. Giske, John Turnidge, Rafael Canton, Gunnar Kahlmeter
Summary: EUCAST is an international committee focused on susceptibility testing, aiming to harmonize clinical breakpoints in Europe and beyond. Its activities include collecting MIC distribution data, developing software, identifying epidemiological cutoff values, and creating testing methods. EUCAST's decisions are subject to public consultation, except for breakpoints of new antimicrobial agents.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Sergio Garcia-Fernandez, Patricia J. Simner, Gina Thomson, Matthew Faron, Ron Bosboom, Arjanne van Griethuijsen, Maria Garcia-Castillo, Renee Harris, Nathan A. Ledeboer, Rafael Canton, Kenneth S. Thomson
Summary: The new BD MAX Check-Points CPO assay showed good performance in detecting KPC and OXA-48 genes, while data on VIM/IMP and NDM tests were insufficient. This detection method can rapidly and accurately identify the most common CPO infections.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2022)
Article
Microbiology
Marta Hernandez-Garcia, Juan Antonio Castillo-Polo, Desiree Gijon Cordero, Blanca Perez-Viso, Maria Garcia-Castillo, Javier Saez de la Fuente, Maria Isabel Morosini, Rafael Canton, Patricia Ruiz-Garbajosa
Summary: The emergence of Klebsiella pneumoniae isolates carrying novel bla(KPC) variants conferring ceftazidime-avibactam (CAZ/AVI) resistance is increasing. The accuracy of phenotypic methods commonly used in routine clinical laboratories for detecting novel K. pneumoniae carbapenemase (KPC) enzymes was evaluated. Whole-genome sequencing (WGS) was used to characterize KPC-ST307-K. pneumoniae isolates before and after CAZ/AVI therapy. Common phenotypic methods failed to detect novel KPC carbapenemases, but rapid molecular or immunochromatography assays were successful, highlighting their relevance in microbiology laboratories.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Editorial Material
Infectious Diseases
Soren Gatermann, Shampa Das, Luc Dubreuil, Christian G. Giske, Gunnar Kahlmeter, Gerard Lina, Christoffer Lindemann, Alasdair MacGowan, Joseph Meletiadis, Gian Maria Rossolini, John Turnidge, Rafael Canton
CLINICAL MICROBIOLOGY AND INFECTION
(2022)
Review
Microbiology
Juan de Dios Caballero, Rafael Canton, Manuel Ponce-Alonso, Marta Maria Garcia-Clemente, Elia Gomez G. de la Pedrosa, Jose Luis Lopez-Campos, Luis Maiz, Rosa del Campo, Miguel Angel Martinez-Garcia
Summary: Microbes, including fungi, play a significant role in the pathogenesis of chronic lung diseases. Next-generation sequencing techniques have revealed complex microbial lung communities, with fungi being an important component. The composition of the lung mycobiome influences the clinical course of chronic respiratory diseases through direct pathogenesis and interactions with bacterial species and the host's physiology.
Review
Respiratory System
Pavel Drevinek, Rafael Canton, Helle Krogh Johansen, Lucas Hoffman, Tom Coenye, Pierre-Regis Burgel, Jane C. Davies
Summary: This review summarizes the main points raised and highlighted during the pre-conference meeting to the 17th European Cystic Fibrosis Society Basic Science Conference. The report covers the latest information and topics related to antimicrobial resistance in cystic fibrosis, as well as advancements in novel anti-infective approaches.
JOURNAL OF CYSTIC FIBROSIS
(2022)
Article
Immunology
Jose Avendano-Ortiz, Manuel Ponce-Alonso, Emilio Llanos-Gonzalez, Hugo Barragan-Prada, Raquel Barbero-Herranz, Roberto Lozano-Rodriguez, Francesc J. Marquez-Garrido, Jose Maria Hernandez-Perez, Maria-Isabel Morosini, Rafael Canton, Rosa del Campo, Eduardo Lopez-Collazo
Summary: We investigated the effects of different lipopolysaccharide (LPS) modifications on colistin resistance and found that adding Ara4N to LPS increases the survival of Klebsiella pneumoniae in the human body and elicits a stronger inflammatory response.
INFECTION AND IMMUNITY
(2023)
Article
Public, Environmental & Occupational Health
Friederike Maechler, Frank Schwab, Sonja Hansen, Michael Behnke, Marc J. Bonten, Rafael Canton, Cristina Diaz Agero, Carolina Fankhauser, Stephan Harbarth, Benedikt D. Huttner, Axel Kola, Petra Gastmeier
Summary: This study aimed to quantify the time delay between screening and initiation of contact isolation for carriers of ESBL-E. The results showed that despite extensive surveillance screening, almost one-third of ESBL-E carriers were not isolated, impeding timely implementation of targeted contact isolation.
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY
(2023)
Article
Infectious Diseases
Juan Antonio Castillo-Polo, Marta Hernandez-Garcia, Maria Isabel Morosini, Blanca Perez-Viso, Cruz Soriano, Raul De Pablo, Rafael Canton, Patricia Ruiz-Garbajosa
Summary: The study aimed to describe the phenotypic and genotypic characteristics of ceftazidime/avibactam- and cefiderocol-resistant KPC-Klebsiella pneumoniae (KPC-Kp) during an outbreak in a medical ICU. The KPC-Kp isolates involved in the outbreak were found to belong to the high-risk clone ST307 and carried the KPC + CTX-M-15 genes. Cloning experiments showed that specific gene mutations may contribute to resistance to ceftazidime/avibactam and cefiderocol.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Review
Microbiology
Teresa M. Coque, Rafael Canton, Ana Elena Perez-Cobas, Miguel D. Fernandez-de-Bobadilla, Fernando Baquero
Summary: Antimicrobial resistance (AMR) is a global health challenge that has been studied and approached from various perspectives, including patient safety, molecular epidemiology, public health, health economics, community psychology, and history of science. However, there is a lack of dialogue between different stakeholders, hindering the development and implementation of effective strategies to combat AMR.
Article
Infectious Diseases
Rafael Canton, Thomas Gottlieb, Geoffrey W. Coombs, Patrick C. Y. Woo, Tony M. Korman, Maria Garcia-Castillo, Denise Daley, Karri A. Bauer, Michael Wong, Dominik J. Wolf, Fakhar Siddiqui, Mary Motyl
Summary: Antimicrobial resistance (AMR) is a major global public health threat, particularly affecting patients in resource-poor settings. Various surveillance programs exist worldwide, and one of the largest is the SMART program, which has been running for 20 years. The SMART database includes data from nearly 500,000 isolates from over 60 countries, and can help monitor and guide clinical treatment.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Review
Medicine, General & Internal
Francisco Javier Candel, Miguel Salavert, Miren Basaras, Marcio Borges, Rafael Canton, Emilia Cercenado, Catian Cilloniz, Angel Estella, Juan M. Garcia-Lechuz, Jose Garnacho Montero, Federico Gordo, Agustin Julian-Jimenez, Francisco Javier Martin-Sanchez, Emilio Maseda, Mayra Matesanz, Rosario Menendez, Manuel Miron-Rubio, Raul Ortiz de Lejarazu, Eva Polverino, Pilar Retamar-Gentil, Luis Alberto Ruiz-Iturriaga, Susana Sancho, Leyre Serrano
Summary: This paper reviews important issues in the approach to community-acquired pneumonia, including updates in etiology, diagnostic guidance, treatment, management, and prevention.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Health Care Sciences & Services
Miquel Angel Sastre-Femenia, Almudena Fernandez-Munoz, Maria Antonia Gomis-Font, Biel Taltavull, Carla Lopez-Causape, Jorge Arca-Suarez, Luis Martinez-Martinez, Rafael Canton, Nieves Larrosa, Jesus Oteo-Iglesias, Laura Zamorano, Antonio Oliver
Summary: A high-resolution analysis of resistance patterns, genomic epidemiology, and resistome of Pseudomonas aeruginosa in Spain over a five-year period reveals a general decrease in resistance but an increase in the proportion of XDR strains producing carbapenemases, with the high-risk clone ST235 becoming more prevalent.
LANCET REGIONAL HEALTH-EUROPE
(2023)
Review
Microbiology
Wai Ting Chan, Maria Pilar Garcillan-Barcia, Chew Chieng Yeo, Manuel Espinosa
Summary: Toxin-antitoxin (TA) systems are important entities found in prokaryotic genomes and play roles in plasmid maintenance, persistence, virulence, and defense against bacteriophages. Type II toxins primarily target bacterial translational machinery. Streptococcus pneumoniae encodes HEPN-RNases as part of the TA systems, while the PezAT family targets cell wall synthesis. Homologues of PezT/Zeta toxins are abundantly distributed among bacterial phyla.
FEMS MICROBIOLOGY REVIEWS
(2023)
