Article
Microbiology
Mona Shaaban, Soha Lotfy Elshaer, Ola A. Abd El-Rahman
Summary: This study aimed to determine the prevalence of β-lactamases, AmpC, and carbapenemases in Proteus mirabilis isolated from clinical specimens. The results showed a high level of antibiotic resistance, with 41.4% of isolates being multidrug-resistant. The most detected genes were bla(SHV), bla(AmpC), and bla(VIM-1). Additionally, some strains produced both ESBL and AmpC, and there were even strains that co-produced ESBL, AmpC, and carbapenemases. Moreover, genetic analysis based on ERIC patterns revealed high genetic diversity.
Article
Biotechnology & Applied Microbiology
Bindu Kiranmayi Chinnam, Subhashini Nelapati, Srinivasa Rao Tumati, Suresh Bobbadi, Venkata Chaitanya Peddada, Bhavana Bodempudi
Summary: Proteus mirabilis is commonly found in soil and water, and can cause serious infections in humans, including complex urinary tract infections. A study analyzing samples from animal-origin foods and animal intestinal samples recovered 232 P. mirabilis isolates, with 72 of them producing beta-lactamase. The genetic relationships between isolates from different sources were identified through further genotyping.
JOURNAL OF FOOD PROTECTION
(2021)
Article
Infectious Diseases
Se Ra Shin, Seong Mi Noh, Woo Kyung Jung, Sook Shin, Young Kyung Park, Dong Chan Moon, Suk-Kyung Lim, Yong Ho Park, Kun Taek Park
Summary: The study conducted in South Korea between 2017 and 2019 identified various ESC-resistant bacterial isolates in companion animals, with predominant species being E. coli, Klebsiella, and others. These isolates displayed diversity in sequence types and resistance genes.
Article
Immunology
Ying Li, Ming Yin, Chengju Fang, Yu Fu, Xiaoyi Dai, Wei Zeng, Luhua Zhang
Summary: Due to high resistance and virulence potential, multidrug-resistant (MDR) P. mirabilis has become a significant pathogen in catheter-associated urinary tract infections, posing a public health risk. This study investigated the resistance characteristics and virulence potential of a collection of clinical isolates. The findings highlight the urgent threat to public health posed by the prevalence of MDR P. mirabilis with high virulence potential. Evaluation: 5 out of 10.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Dominika Drzewiecka, Malgorzata Siwinska, Sof'ya N. Senchenkova, Evgeniya A. Levina, Alexander S. Shashkov, Yuriy A. Knirel
Summary: Two Proteus mirabilis strains, Kr1 and Ks20, were isolated from wound and skin samples of infected patients in central Poland. They have a unique O serotype that is not recognized by Proteus O1-O83 antisera. The O-specific polysaccharide structure of P. mirabilis Kr1 was determined through chemical analysis and NMR spectroscopy, and based on their serological features and chemical data, Kr1 and Ks20 were proposed as candidates for a new O-serogroup, O84, in the genus Proteus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Public, Environmental & Occupational Health
Marimuthu Ragavan Rameshkumar, Narasingam Arunagirinathan, Balasubramanian Senthamilselvan, Chinnambedu Ravichandran Swathirajan, Sunil Suhas Solomon, Ramachandran Vignesh, Pachamuthu Balakrishnan, Reem M. Aljowaie, Khalid S. Almaary, Tse-Wei Chen
Summary: This study found that Gram-negative bacterial isolates from HIV patients in South India show resistance to multiple antibiotics, with high levels of expression of ESBL genes indicating a high prevalence of multidrug-resistant bacteria in this population.
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2021)
Article
Infectious Diseases
Ricardo Oliveira, Joana Castro, Sonia Silva, Hugo Oliveira, Maria Jose Saavedra, Nuno Filipe Azevedo, Carina Almeida
Summary: This study identified a diverse pool of beta-lactam resistance genetic determinants in 102 multi-drug resistant K. pneumoniae isolates from patients in northern Portugal, leading to high levels of resistance to most beta-lactam antibiotics. The co-occurrence of multiple beta-lactam resistance genes in the majority of isolates serves as a serious warning of the problem of antimicrobial resistance.
Article
Infectious Diseases
Matheus Silva Sanches, Luana Carvalho Silva, Caroline Rodrigues da Silva, Victor Hugo Montini, Bruno Henrique Dias de Oliva, Gustavo Henrique Migliorini Guidone, Mara Correa Lelles Nogueira, Maisa Fabiana Menck-Costa, Renata Katsuko Takayama Kobayashi, Eliana Carolina Vespero, Sergio Paulo Dejato Rocha
Summary: The study aimed to evaluate the prevalence of antimicrobial resistance and clonal relationships in Proteus mirabilis isolated from different sources. Results showed that chicken meat had the highest multidrug resistance, followed by pork and community-acquired urinary tract infections (UTI-CA), while beef had relatively few multidrug-resistant strains. Various antimicrobial resistance genes were detected, especially in chicken meat and UTI-CA isolates. Molecular typing revealed genetic similarity between chicken meat and UTI-CA isolates, indicating that chicken could be a source of P. mirabilis causing UTI-CA. It was concluded that meat, especially chicken, can be an important source of multidrug-resistant P. mirabilis dissemination in the community.
Article
Microbiology
Xiaoyun Qu, Jie Zhou, Haoqi Huang, Wen Wang, Yingping Xiao, Biao Tang, Hanlin Liu, Chenggang Xu, Xingning Xiao
Summary: A study in Zhejiang Province, China, found that Proteus mirabilis contamination in pig farms showed high resistance to multiple antibiotics and strong biofilm formation. Whole genome sequencing revealed the presence of various antimicrobial resistance genes and virulence factors. These findings highlight the need for stricter regulation of veterinary antibiotic use.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Jungsun Park, Eunkyung Shin, Wooju Kang, Joohyun Han, Jaeil Yoo, Jung-Sik Yoo, Dong-Hyun Roh, Junyoung Kim
Summary: This study characterized ESBL-producing pathogenic E. coli CC10 strains isolated from patients with diarrhea and found that CTX-M-15-producing E. coli O25:H16 isolates were the major type associated with CC10 distribution over the past decade. Continuous monitoring is crucial to prevent the further dissemination of resistant ESBL-producing E. coli CC10 strains.
Article
Immunology
Ibrahim Bitar, Tamara Salloum, Georgi Merhi, Jaroslav Hrabak, George F. Araj, Sima Tokajian
Summary: This study investigated the resistance patterns and beta-lactamases associated with ceftolozane/tazobactam (C/T) resistance in multi-drug resistant Pseudomonas aeruginosa clinical isolates. Whole-genome sequencing revealed the presence of acquired OXA beta-lactamases and metallo-beta-lactamases as the main drivers of C/T resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Amelie Lombes, Remy A. Bonnin, Frederic Laurent, Helene Guet-Revillet, Emmanuelle Bille, Vincent Cattoir, Marie-Sarah Fangous, Cecile Le Brun, Vincent Fihman, Frederic Janvier, Marie-Pierre Otto, Anais Potron, Stephane Corvec, Louise Ruffier D'Epenoux, Assaf Mizrahi, Laurent Dortet
Summary: This multicentric study in French hospitals found that 26.9% of amoxicillin-clavulanate-resistant Proteus mirabilis isolates produced the OXA-23 carbapenemase. The study also identified a screening cutoff of <11 mm for the inhibition zone diameter around the amoxicillin-clavulanate disc to accurately detect these OXA-23 producers. Whole-genome sequencing confirmed that these OXA-23 producers belonged to the same lineage known to disseminate OXA-23 or OXA-58 in P. mirabilis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Wenjun Lu, Quedan Qiu, Keda Chen, Rongqing Zhao, Qingcao Li, Qiaoping Wu
Summary: The study found that class 1, 2, and 3 integrons are present in Proteus mirabilis isolates from patients, with class 2 integrons having low distribution but relatively conserved molecular characteristics. Three P. mirabilis isolates with functional class 2 integrons shared the same gene cassette but exhibited differences in homology and drug sensitivity.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Infectious Diseases
Mai A. Amer, Reham Wasfi, Ahmed S. Attia, Mohamed A. Ramadan
Summary: Proteus mirabilis is a common cause of catheter associated urinary tract infections, with increasing antibiotic resistance prompting the search for new treatment options. Extracts from Enterobacter sp. isolated from Egyptian soil samples showed potent antivirulence activity against P. mirabilis, particularly in antagonizing biofilm activity. The study highlights the potential of using these extracts as promising antivirulence agents against P. mirabilis uropathogens and as a potential therapy for UTIs.
Article
Pharmacology & Pharmacy
Engy Elekhnawy, Alanood S. Almurshedi, Dalia H. Abdelkader, Thanaa A. El-Masry, Basmah N. Aldosari, Maisra M. El-Bouseary, Ahmed A. Donia, Walaa A. Negm
Summary: Currently, antimicrobial resistance is spreading at an accelerated rate, leading researchers to investigate alternative treatments. This study evaluated the antibacterial properties of zinc-oxide nanoparticles synthesized from Cycas circinalis against Proteus mirabilis clinical isolates. The nanoparticles were found to be effective in inhibiting bacterial growth in vitro and in vivo, reducing bacterial count and improving survival rates in a mouse model. The study suggests that zinc-oxide nanoparticles could be a potential solution to bacterial infections caused by Proteus mirabilis.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Gastroenterology & Hepatology
Saein Kim, Sunghwan Yoo, Jung Il Lee, Sora Kim, Hye Young Chang, Dokyun Kim, Seok Hoon Jeong, Kwan Sik Lee, Hyun Woong Lee
Summary: This study investigated the predictive value of the indirect ratio of anti-HBc IgG in HBsAg seroclearance in patients with nucleos(t)ide analogue-induced HBeAg seroclearance. The results showed that baseline anti-HBc IgG levels < 11 RLU, age >= 50 years, and ALT >= 40 IU/L may be associated with HBsAg seroclearance in these patients.
DIGESTIVE DISEASES AND SCIENCES
(2022)
Article
Medical Laboratory Technology
Si Hyun Kim, Gyung-Hye Sung, Eun Hee Park, In Yeong Hwang, Gyu Ri Kim, Sae Am Song, Hae Kyung Lee, Young Uh, Young Ah Kim, Seok Hoon Jeong, Jong Hee Shin, Kyeong Seob Shin, Jaehyeon Lee, Joseph Jeong, Young Ree Kim, Dongeun Yong, Miae Lee, Yu Kyung Kim, Nam Hee Ryoo, Seungok Lee, Jayoung Kim, Sunjoo Kim, Hyun Soo Kim, Jeong Hwan Shin
Summary: This study investigated the distribution and antimicrobial resistance of Salmonella isolates collected in Korea from 2016 to 2017. The results showed that the most prevalent serogroups were C and B, and there were multiple serotypes of Salmonella with many strains showing resistance to antibiotics. Periodic investigations of Salmonella serotypes and antimicrobial resistance are necessary.
ANNALS OF LABORATORY MEDICINE
(2022)
Article
Medical Laboratory Technology
Sewhan Um, Jaeyoung Her, Si Hyun Kim, Sae Am Song, Young Nam Kim, Jeong Hwan Shin
Summary: In this study, the diagnostic performance of the BD Max GBS assay for detecting GBS using vaginal and rectal specimens was evaluated. The sensitivity of direct BDM was 100% for rectal specimens and 75.0% for vaginal specimens. The overall agreement between direct BDM and culture was 98.9%, with a sensitivity and specificity of 98.5% and 99.0%, respectively. Discrepant results were obtained for four specimens.
ANNALS OF LABORATORY MEDICINE
(2022)
Article
Multidisciplinary Sciences
Yong Chan Kim, Heun Choi, Young Ah Kim, Yoon Soo Park, Young Hee Seo, Hyukmin Lee, Kyungwon Lee
Summary: Understanding the risk factors and microbiological features in recurrent E. coli BSI can aid clinicians in their management planning. Data from 2017 to 2018 were collected to assess antimicrobial resistance rates and molecular epidemiology. Risk factors for recurrence included vascular catheterization, ESBL phenotype, and high SOFA score. The highest proportion of ST131 and ESBL genotype was observed in early onset recurrent BSI. Whole genome sequencing revealed the presence of various virulence factors, resistant genes, and plasmid types in all types of BSI. This knowledge is important for clinicians in managing recurrent E. coli BSI.
Article
Medical Laboratory Technology
Yu Jeong Choi, Young Ah Kim, Kim Junglim, Seok Hoon Jeong, Jong Hee Shin, Kyeong Seob Shin, Jeong Hwan Shin, Young Ree Kim, Hyun Soo Kim, Young Uh, Nam Hee Ryoo
Summary: IMP-6-producing Pseudomonas aeruginosa sequence type 235 is a dominant clone of carbapenemase-producing P. aeruginosa in Korea. The resistance rate of P. aeruginosa isolates from blood cultures has increased and there has been a shift in the molecular epidemiology of carbapenemase-producing P. aeruginosa.
ANNALS OF LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Yangsoon Lee, Hye Gyung Bae, Dongju Won, Woobin Yun, Hyukmin Lee, Jong Rak Choi, Young Uh, Kyungwon Lee
Summary: In this study, the distribution of GBS isolates in pregnant Korean women was investigated. CC1 was the dominant lineage, followed by CC19, CC12, and CC17. The low prevalence of CC17 GBS colonization may explain the low frequency of neonatal GBS infections. Whole-genome sequencing is a useful tool for predicting antimicrobial susceptibility and capsular serotypes in GBS isolates.
ANNALS OF LABORATORY MEDICINE
(2023)
Article
Infectious Diseases
Min Hyuk Choi, Dokyun Kim, Kyoung Hwa Lee, Jae Hwa Cho, Seok Hoon Jeong
Summary: This study aimed to evaluate the changes in pathogens causing hospital-acquired bacterial pneumonia (HABP), their antimicrobial resistance patterns, and the risk factors for 28-day all-cause mortality in patients with HABP. The annual incidence of Staphylococcus aureus HABP decreased while that of Klebsiella pneumoniae HABP increased significantly. The resistance rate of S. aureus to methicillin decreased, while the non-susceptibility rate of K. pneumoniae to carbapenems increased. HABP caused by certain pathogens and preceded by COVID-19 was associated with increased mortality and incidence of bacteraemic pneumonia.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Immunology
Han Sang Kim, Chang Gon Kim, Won Kyu Kim, Kyung-A Kim, Jinseon Yoo, Byung Soh Min, Soonmyung Paik, Sang Joon Shin, Hyukmin Lee, Kyungwon Lee, Hoguen Kim, Eui-Cheol Shin, Tae-Min Kim, Joong Bae Ahn
Summary: This study found that Fusobacterium nucleatum (FN) in colorectal cancer tissues is associated with immune regulation in the tumor microenvironment, and its infection is correlated with poor prognosis and altered distribution of immune cells.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Letter
Public, Environmental & Occupational Health
Si Hyun Kim, Gyu Ri Kim, Young Ree Kim, Young Ah Kim, Hyun Soo Kim, Kyeong Seob Shin, Jong Hee Shin, Young Uh, Seok Hoon Jeong, Namhee Ryoo, Jeong Hwan Shin
JOURNAL OF INFECTION AND PUBLIC HEALTH
(2023)
Article
Microbiology
Jungho Park, Naeun Kwak, Jong-Chan Chae, Eun-Jeong Yoon, Seok Hoon Jeong
Summary: Tuberculosis (TB) and nontuberculous mycobacteria (NTM) infections are ongoing threats to public health. A two-step PCR method was developed to accurately diagnose patients with suspected mycobacterial infections, distinguishing between TB and NTM, and identifying specific NTM species. This method showed promising results, comparable to commercially available PCR kits, and enabled the identification of mycobacterial species in most NTM infection cases.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Seung A. Byun, Yong Jun Kwon, Ga Yeong Lee, Min Ji Choi, Seok Hoon Jeong, Dokyun Kim, Min Hyuk Choi, Seung-Jung Kee, Soo Hyun Kim, Myung Geun Shin, Eun Jeong Won, Jong Hee Shin
Summary: This study analyzed the virulence traits and azole resistance mechanisms of 104 Candida auris isolates collected from Korean hospitals from 1996 to 2022. The results showed a high fluconazole resistance rate among C. auris isolates in Korean hospitals, with two clades identified and clade II isolates exhibiting higher resistance. The isolates from different clades were sensitive to other drugs, and clade II isolates displayed reduced thermotolerance, competitive growth, and virulence.
Article
Medical Laboratory Technology
Gyu Ri Kim, Eun-Young Kim, Si Hyun Kim, Hae Kyung Lee, Jaehyeon Lee, Jong Hee Shin, Young Ree Kim, Sae Am Song, Joseph Jeong, Young Uh, Yu Kyung Kim, Dongeun Yong, Hyun Soo Kim, Sunjoo Kim, Young Ah Kim, Kyeong Seob Shin, Seok Hoon Jeong, Namhee Ryoo, Jeong Hwan Shin
Summary: After the introduction of PCV13 in Korea, there have been changes in the serotype distribution and antimicrobial resistance of invasive S. pneumoniae causing IPD. Non-PCV13 serotypes are increasing, and there are differences in antimicrobial resistance according to specific serotypes. Continuous monitoring of serotypes and antimicrobial resistance is crucial for the appropriate management of S. pneumoniae infections.
ANNALS OF LABORATORY MEDICINE
(2023)
Letter
Medical Laboratory Technology
Kyunghee Yu, Junhyup Song, Dokyun Kim, Yongjung Park, Seok Hoon Jeong
ANNALS OF LABORATORY MEDICINE
(2023)
Article
Infectious Diseases
Min Hyuk Choi, Dokyun Kim, Yongjung Park, Seok Hoon Jeong
Summary: This study aimed to determine the risk factors for the progression of UTI to BSI and evaluate mortality-associated factors in patients with UT-BSI. The study found that UTI caused by Candida species was significantly associated with high 30-day mortality. Furthermore, considering the increasing age of patients and more frequent use of indwelling urologic devices, UT-BSIs caused by microorganisms other than Escherichia coli could become a more serious medical burden in the future.
JOURNAL OF INFECTION
(2022)