Article
Infectious Diseases
Guillaume Millette, David Lalonde Seguin, Charles Isabelle, Suzanne Chamberland, Jean-Francois Lucier, Sebastien Rodrigue, Andre M. Cantin, Francois Malouin
Summary: Prototypic Staphylococcus aureus and their small-colony variants (SCVs) are predominant in cystic fibrosis (CF) patients. We characterized S. aureus isolates from adult CF patients over several years, finding that a majority of patients were positive for SCVs. Whole-genome sequencing revealed the presence of both prototypical and SCV-related clones in some patients, and SCVs showed characteristics that could contribute to resistance acquisition.
Article
Biochemistry & Molecular Biology
Chad Normandin, Pierre-Luc Boudreault
Summary: Tomatidine, a newly discovered potential antibiotic agent, has low natural abundance and high cost, prompting researchers to develop an efficient and scalable synthesis method to produce multi-grams of the compound. This synthesis involves key coupling reactions and sequential transformations to achieve a high yield in a cost-effective and scalable manner.
Editorial Material
Immunology
Anthony J. Fischer
Summary: Staphylococcus aureus, the most common pathogen in cystic fibrosis (CF) patients, evolves small colony variants (SCVs) during chronic airway infections. Observational studies have shown a correlation between SCVs and deteriorating lung function in CF. A new experimental study provides intriguing evidence that SCVs elicit greater inflammation than normal colonies in CF rats.
INFECTION AND IMMUNITY
(2022)
Article
Cell & Tissue Engineering
Y. Cai, C. Huang, X. Chen, Y. Chen, Z. Huang, C. Zhang, W. Zhang, X. Fang
Summary: This study aimed to investigate the role of Staphylococcus aureus small colony variants (SCVs) in intraosseous invasion and colonization in patients with periprosthetic joint infection (PJI). The results showed that SCVs have a greater ability to invade and colonize bone, and there are significant differences in gene expression profiles between chronic and acute PJI. These findings are important for understanding the pathogenesis of chronic infections.
BONE & JOINT RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Henan Guo, Yucui Tong, Junhao Cheng, Zaheer Abbas, Zhongxuan Li, Junyong Wang, Yichen Zhou, Dayong Si, Rijun Zhang
Summary: The overuse of antibiotics leading to drug-resistance and multidrug-resistance has become a major concern. Staphylococcus aureus, as a representative of drug-resistant pathogens, can cause a variety of infections and adapt to diverse environments through phenotypic changes like biofilm and SCVs. This article reviews the development of drug-resistance, explores classical mechanisms, and focuses on the non-classical resistance mechanisms triggered by biofilm and SCVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Infectious Diseases
Xiangkuo Zheng, Renchi Fang, Chong Wang, Xuebin Tian, Jie Lin, Weiliang Zeng, Tieli Zhou, Chunquan Xu
Summary: The study revealed that S. aureus SCVs share rifampicin resistance mechanisms with parental strains but exhibit significant differences in biological characteristics, showing reduced susceptibility to other antibiotics and altered growth ability, biofilm formation, and virulence profiles. Additionally, SCVs incur a fitness cost during phenotype switch.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Critical Care Medicine
Anthony J. Fischer, Sachinkumar B. Singh, Mason M. LaMarche, Lucas J. Maakestad, Zoe E. Kienenberger, Tahuanty A. Pena, David A. Stoltz, Dominique H. Limoli
Summary: Staphylococcus aureus and Pseudomonas aeruginosa are both abundant in sputum cultures of cystic fibrosis patients. Contrary to common assumption, there is no rapid replacement of S. aureus by P. aeruginosa. Many CF patients have long-term coinfection with these organisms.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Article
Medicine, Research & Experimental
Kyle Rosenke, Atsushi Okumura, Matthew C. Lewis, Friederike Feldmann, Kimberly Meade-White, W. Forrest Bohler, Amanda Griffin, Rebecca Rosenke, Carl Shaia, Michael A. Jarvis, Heinz Feldmann
Summary: MK-4482 demonstrates efficacy against earlier SARS-CoV-2 lineages and shows significant inhibitory effect on the replication of the Omicron variant, reducing lung disease and viral antigen load.
Article
Infectious Diseases
Martin Vestergaard, Sahar Roshanak, Hanne Ingmer
Summary: ATP synthase inhibitors have shown selective inhibitory activity against pathogenic microorganisms where ATP synthase is essential, but some bacteria (e.g. S. aureus) do not rely on ATP synthase. Therefore, using ATP synthase inhibitors in combination with antimicrobial peptides may provide new therapeutic options for bacterial pathogens such as S. aureus.
Article
Immunology
Ruchi Yadav, Quan-Zhen Li, Hanwen Huang, S. Louis Bridges, J. Michelle Kahlenberg, Arlene A. Stecenko, Balazs Rada
Summary: This study identified several new autoantibodies in the blood of CF patients and found associations with neutrophil components, diabetes, lung disease severity, and P. aeruginosa infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Shengzhe Zhou, Yifan Rao, Jia Li, Qiaoyi Huang, Xiancai Rao
Summary: This review summarizes the significance of genetic mutations involved in diverse biosynthesis pathways that contribute to the promotion of small-colony variants (SCVs) of Staphylococcus aureus (Sau). It addresses the persistent infections caused by Sau-SCVs and discusses the prospects and challenges in their treatment. The progress in managing Sau-SCVs may provide guidance for addressing long-term and recurrent infections caused by other bacterial SCVs.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Veterinary Sciences
Jinhuan Liu, Mujie Ju, Yifei Wu, Nannan Leng, Samah Attia Algharib, Wanhe Luo
Summary: Florfenicol composite nanogels were prepared for targeted delivery to SCV infected sites. The nanogels exhibited sustained drug release, showed good antibacterial activity against SCVs, and had therapeutic efficacy against SCV mastitis. Furthermore, the nanogels demonstrated good biocompatibility and safety.
JOURNAL OF VETERINARY SCIENCE
(2022)
Article
Microbiology
Maureen Kleinhenz, Pavani Beesetty, Ching Yang, Zhaotao Li, Christopher P. Montgomery
Summary: Recurrent Staphylococcus aureus infections are common, but no approved vaccine is available. Antibiotic therapy resolves infection but fails to elicit protective immunity. Combined vaccination and antibiotics can treat infection and generate durable protective immunity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Medical Laboratory Technology
Changhang Min, Haichen Wang, Fengjun Xia, Mengli Tang, Jun Li, Yongmei Hu, Qingya Dou, Mingxiang Zou
Summary: The study investigated the prevalence and characteristics of Staphylococcus aureus small colony variants (SCVs), finding a low detection rate in wound samples. SCVs have significantly different characteristics from classic S. aureus and may lead to chronic infections and mild inflammation. There was no clonal epidemic of SCVs in the hospital.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2022)
Article
Microbiology
Fengjia Chen, Jing Zhang, Hyun Jung Ji, Min-Kyu Kim, Kyoung Whun Kim, Jong-Il Choi, Seung Hyun Han, Sangyong Lim, Ho Seong Seo, Ki Bum Ahn
Summary: Deinococcus radiodurans is a highly resistant bacterium with cellular constituents, such as DeinoPol, playing a key role in inhibiting Staphylococcus aureus colonization and biofilm formation. DeinoPol interferes with S. aureus biofilm formation by disrupting production of poly-N-acetylglucosamine, suggesting its potential application in preventing/treating S. aureus biofilm-related infections or inflammatory diseases.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Maxime Lamontagne Boulet, Charles Isabelle, Isabelle Guay, Eric Brouillette, Jean-Philippe Langlois, Pierre-Etienne Jacques, Sebastien Rodrigue, Ryszard Brzezinski, Pascale B. Beauregard, Kamal Bouarab, Kumaraswamy Boyapelly, Pierre-Luc Boudreault, Eric Marsault, Francois Malouin
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2018)
Article
Microbiology
S. Pichette-Jolette, G. Millette, E. Demontier, D. Bran-Barrera, M. Cyrenne, C. Ster, D. Haine, G. Keefe, F. Malouin, J. P. Roy
VETERINARY MICROBIOLOGY
(2019)
Article
Microbiology
Evelyne Lacasse, Eric Brouillette, Audrey Larose, Thomas R. Parr, Aileen Rubio, Francois Malouin
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2019)
Letter
Microbiology
Julie Jeukens, Luca Freschi, Irena Kukavica-Ibrulj, Jean-Guillaume Emond-Rheault, Christian Allard, Jean Barbeau, Andre Cantin, Steve J. Charette, Eric Deziel, Francois Malouin, Julie Milot, Dao Nguyen, Clara Popa, Brian Boyle, Roger C. Levesque
JOURNAL OF CLINICAL MICROBIOLOGY
(2019)
Article
Multidisciplinary Sciences
Julie Cote-Gravel, Eric Brouillette, Francois Malouin
Article
Multidisciplinary Sciences
Jocelyn Bernier-Lachance, Julie Arsenault, Valentine Usongo, Eric Parent, Josee Labrie, Mario Jacques, Francois Malouin, Marie Archambault
Article
Microbiology
Guillaume Millette, Jean-Philippe Langlois, Eric Brouillette, Eric H. Frost, Andre M. Cantin, Francois Malouin
FRONTIERS IN MICROBIOLOGY
(2019)
Article
Microbiology
Jean-Philippe Langlois, Guillaume Millette, Isabelle Guay, Alexis Dube-Duquette, Suzanne Chamberland, Pierre-Etienne Jacques, Sebastien Rodrigue, Kamal Bouarab, Eric Marsault, Francois Malouin
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Fabrice Betoudji, Taha Abd El Rahman, Marvin J. Miller, Manuka Ghosh, Mario Jacques, Kamal Bouarab, Francois Malouin
Article
Pharmacology & Pharmacy
Jose Carratala, Eric Brouillette, Naroa Serna, Alejandro Sanchez-Chardi, Julieta M. Sanchez, Antonio Villaverde, Anna Aris, Elena Garcia-Fruitos, Neus Ferrer-Miralles, Francois Malouin
Article
Immunology
Celine Ster, Marianne Allard, Julie Cote-Gravel, Simon Boulanger, Pierre Lacasse, Francois Malouin
Summary: The study developed a vaccine formulation for Staphylococcus aureus mastitis using recombinant proteins and adjuvants, successfully inducing immune responses in cows. Different antigens in the vaccine elicited different immune responses, with one antigen causing a balanced immune response and others causing a predominant Th2 response. Vaccinated cows showed a sustained reduction in milk somatic cell counts, indicating a correlation between somatic cell counts and Staphylococcus aureus counts in milk. Further research is needed to understand how different antigens stimulate varying immune responses using the same adjuvant.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
S. Bennett, I. Fliss, L. Ben Said, F. Malouin, P. Lacasse
Summary: The study evaluated the reduction of bacterial populations through the application of bacteriocin-based teat formulas. The results showed that nisin and the bacteriocin consortium showed the most promise as a teat disinfectant by reducing staphylococci, streptococci, and total bacteria counts.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Infectious Diseases
Guillaume Millette, Evelyne Lacasse, Renaud Binette, Veronique Belley, Louis-Philippe Chaumont, Celine Ster, Francis Beaudry, Kumaraswamy Boyapelly, Pierre-Luc Boudreault, Francois Malouin, Amira A. Moawad, Hosny El-Adawy, Heinrich Neubauer
Summary: Staphylococcus aureus is a major pathogen causing bovine mastitis, and the development of new antibiotics for veterinary use is important due to its virulence and antibiotic resistance. One such antibiotic, PC206, has been found to have specific narrow-spectrum activity against S. aureus and shows promising results in in vitro and in vivo experiments.
Review
Biochemistry & Molecular Biology
Julien A. Delbrouck, Michael Desgagne, Christian Comeau, Kamal Bouarab, Francois Malouin, Pierre-Luc Boudreault
Article
Veterinary Sciences
Auemphon Mordmuang, Eric Brouillette, Supayang Piyawan Voravuthikunchai, Francois Malouin
VETERINARY RESEARCH
(2019)