Article
Microbiology
Wendy Kloezen, Ria J. Melchers, Panagiota-Christina Georgiou, Johan W. Mouton, Joseph Meletiadis
Summary: The combination of taniborbactam and cefepime showed potent activity against ESBL-producing strains, restoring susceptibility in all Enterobacterales and two-thirds of P. aeruginosa isolates.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Kamrul Islam, Fekade B. Sime, Steven C. Wallis, Michelle J. Bauer, Brian M. Forde, Patrick Harris, Tahmina Shirin, Zakir H. Habib, Meerjady S. Flora, Jason A. Roberts
Summary: This study compared the pharmacodynamics of piperacillin/tazobactam and meropenem against ESBL-producing and non-producing E. coli. The results suggest that piperacillin/tazobactam may be a potential alternative to carbapenems in treating urosepsis caused by ESBL-producing E. coli.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2022)
Article
Infectious Diseases
Elena Sendra, Inmaculada Lopez Montesinos, Alicia Rodriguez-Alarcon, Juan Du, Ana Siverio-Pares, Mar Arenas-Miras, Esperanza Canas-Ruano, Nuria Prim, Xavier Duran-Jorda, Fabiola Blasco-Hernando, Enric Garcia-Alzorriz, Francesc Cots, Olivia Ferrandez, Silvia Gomez-Zorrilla
Summary: This study compared the clinical characteristics, outcomes, and economic differences between complicated urinary tract infections caused by extensively drug-resistant Pseudomonas aeruginosa and extended-spectrum beta-lactamase-producing Klebsiella pneumoniae. The results showed that patients with XDR P. aeruginosa cUTI more frequently received inappropriate empirical therapy, while ESBL-K. pneumoniae cUTI was associated with worse eradication rates, higher recurrence, and higher infection-related readmission. XDR P. aeruginosa cUTI was associated with worse early clinical cures and more antibiotic side effects, but no differences in mortality or hospitalization costs were observed.
Article
Infectious Diseases
Hongyu Chen, Huirong Mai, Bruno Lopes, Feiqiu Wen, Sandip Patil
Summary: This study identified carbapenem- and colistin-resistant multi-drug resistant Pseudomonas aeruginosa in paediatric patients with leukaemia in Shenzhen, China. It highlights the need for continuous surveillance of high-risk clones of multi-drug resistant P. aeruginosa. Prudent use of antibiotics based on local antimicrobial susceptibility and clinical characteristics can help in reducing mortality in immunocompromised patients.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Chemistry, Medicinal
Ruihan Dong, Hongpeng Yang, Chengwei Ai, Guihua Duan, Jianxin Wang, Fei Guo
Summary: A novel multichannel deep neural network, DeepBLI, was developed for screening β-lactamase inhibitors. Compared to state-of-the-art methods, DeepBLI showed better performance, indicating its potential in antibacterial drug development and contribution to antibiotic-resistant therapeutics.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Article
Microbiology
Leilei Wang, Xuefei Zhang, Xun Zhou, Yingmin Bi, Minggui Wang, Qinglan Guo, Fan Yang
Summary: Eleven bla(PER-1)-positive Pseudomonas aeruginosa clinical isolates exhibited variable susceptibility to ceftazidime-avibactam (CZA). The genetic contexts of bla(PER-1) were identical except for one isolate, which showed increased resistance due to an insertion in the upstream region of bla(PER-1). The variability in bla(PER-1) promoter activity partially explains the different susceptibility to CZA in PER-producing isolates.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Laurent Poirel, Jose-Manuel Ortiz de la Rosa, Mustafa Sadek, Patrice Nordmann
Summary: The study evaluated the susceptibility of broad-spectrum beta-lactamases to various antibiotics, and found that clavulanic-acid-inhibited extended-spectrum beta-lactamases significantly affected the susceptibility to ceftazidime-avibactam, ceftolozane-tazobactam, and cefiderocol.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Immunology
Pranita D. Tamma, Samuel L. Aitken, Robert A. Bonomo, Amy J. Mathers, David van Duin, Cornelius J. Clancy
Summary: This guidance document provides recommendations for the treatment of antimicrobial-resistant infections caused by ESBL-E, CRE, and DTR-P. aeruginosa, with a focus on the United States. It discusses empiric treatment selection, duration of therapy, and other management considerations for both adults and children. The current version of the guidance is valid as of September 17, 2020, and will be updated periodically.
CLINICAL INFECTIOUS DISEASES
(2021)
Article
Infectious Diseases
Se Ra Shin, Seong Mi Noh, Woo Kyung Jung, Sook Shin, Young Kyung Park, Dong Chan Moon, Suk-Kyung Lim, Yong Ho Park, Kun Taek Park
Summary: The study conducted in South Korea between 2017 and 2019 identified various ESC-resistant bacterial isolates in companion animals, with predominant species being E. coli, Klebsiella, and others. These isolates displayed diversity in sequence types and resistance genes.
Article
Chemistry, Multidisciplinary
Mitchell A. Jeffs, Rachel A. V. Gray, Prameet M. Sheth, Christopher T. Lohans
Summary: A luminescent-based biosensor has been developed to quantify beta-lactamase inhibition in bacterial cells. This method takes into account factors such as membrane permeability and can be used to identify new beta-lactamase inhibitors.
CHEMICAL COMMUNICATIONS
(2023)
Article
Microbiology
Pablo A. Fraile-Ribot, Javier Fernandez, Maria A. Gomis-Font, Lorena Forcelledo, Xavier Mulet, Carla Lopez-Causape, Antonio Oliver
Summary: The study investigated the mechanisms behind the in vivo switch in resistance phenotype of P. aeruginosa after ceftazidime-avibactam treatment. It was found that a single SNP difference can lead to a change in resistance profile, reverting a carbapenemase phenotype back into an ESBL phenotype. Through genetic analysis, it was discovered that certain mutations can alter the resistance patterns of bacteria in vivo.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
V Bratic, S. Mihaljevic, Z. Verzak, E. Plesko, A. Lukic, M. Cacic, B. Bedenic
Summary: The prophylactic use of broad-spectrum antibiotics in surgery may lead to colonization of the oral cavity and lower respiratory tract with Gram-negative bacteria, including multidrug-resistant or extensively drug-resistant organisms. The study found that the bacterial types and resistance mechanisms detected in the oral cavity were similar to those in tracheal aspirates, suggesting that oral swabs can predict the colonization of the lower respiratory tract with resistant Gram-negative organisms and the risk of developing ventilator-associated pneumonia.
LETTERS IN APPLIED MICROBIOLOGY
(2021)
Review
Infectious Diseases
Huan Zhang, Beibei Liang, Jin Wang, Yun Cai
Summary: This study compared the efficacy of non-carbapenem beta-lactam/beta-lactamase inhibitors (BLBLIs) with carbapenems for the treatment of UTIs caused by ESBL-PE. The results showed no significant difference in clinical success and mortality between the two treatment groups, but a slightly higher rate of microbiological success was observed in the BLBLIs group.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Article
Infectious Diseases
Mihret Tilahun, Alemu Gedefie, Habitye Bisetegn, Habtu Debash
Summary: The high prevalence of extended-spectrum beta-lactamase and carbapenemase producing Acinetobacter species and Pseudomonas aeruginosa among hospitalized patients in Dessie Comprehensive Specialized Hospital, Ethiopia is a major concern. Antimicrobial resistance testing revealed a high rate of multidrug resistance among the isolated bacteria.
INFECTION AND DRUG RESISTANCE
(2022)
Article
Microbiology
Maria A. Gomis-Font, Cristina Pitart, Ester del Barrio-Tofino, Yuliya Zboromyrska, Sara Cortes-Lara, Xavier Mulet, Francesc Marco, Jordi Vila, Carla Lopez-Causape, Antonio Oliver
Summary: A Pseudomonas aeruginosa isolate recovered from a patient with ventilator-associated pneumonia after COVID-19 infection showed resistance to multiple antibiotics due to various gene mutations, including modifications in MexD substrate specificity.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Toshiharu Sasaki, Yohei Doi
Summary: This study investigated cases of S. lugdunensis bacteremia and found that the clinical significance of single-positive cases is uncertain. Among all cases, only a small subset of low-risk patients did not require antimicrobial therapy, and they had no adverse clinical consequences.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2023)
Letter
Infectious Diseases
Jiachen Li, Siyuan Feng, Yuhui Chen, Meiling Yu, Wenjing Wei, Guo-Bao Tian
Article
Infectious Diseases
Brian N. Malley, Jonathan Yabes, Elizabeth H. Gimbel, Chung-Chou H. Chang, Donald A. Yealy, Michael Fine, Derek M. Angus, David T. F. Huang
Summary: The Procalcitonin Antibiotic Consensus Trial (ProACT) found that providing a procalcitonin antibiotic prescribing guideline to hospital-based clinicians did not reduce antibiotic use. The study aimed to determine the threshold adherence rate for reducing antibiotic use and to explore opportunities to increase adherence.
BMC INFECTIOUS DISEASES
(2023)
Article
Immunology
Hiroshi Mukae, Hiroshi Yotsuyanagi, Norio Ohmagari, Yohei Doi, Hiroki Sakaguchi, Takuhiro Sonoyama, Genki Ichihashi, Takao Sanaki, Keiko Baba, Yuko Tsuge, Takeki Uehara
Summary: This study evaluated the efficacy and safety of ensitrelvir for mild-to-moderate COVID-19 during the Omicron epidemic. The results showed that ensitrelvir treatment significantly reduced the SARS-CoV-2 titer compared to placebo. However, there was no significant difference in the total score of the 12 COVID-19 symptoms. Overall, ensitrelvir demonstrated favorable antiviral efficacy and potential clinical benefit with an acceptable safety profile.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Clement Kin-Ming Tsui, Fatma Ben Abid, Khalil Al Ismail, Christi Lee McElheny, Muna Al Maslamani, Ali S. Omrani, Yohei Doi
Summary: The emergence of carbapenem-resistant, hypervirulent Klebsiella pneumoniae is a new threat to health care. We studied the molecular epidemiology of carbapenem-resistant Klebsiella pneumoniae isolates in Qatar using whole-genome sequence data. We also characterized the prevalence and genetic basis of hypervirulent phenotypes and established the virulence potential using a Galleria mellonella model.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Infectious Diseases
Rimi Tanii, Sohei Harada, Hiroki Saito, Koh Okamoto, Yohei Doi, Masahiro Suzuki
Summary: This article reports a fatal case of respiratory and bloodstream infection caused by K. variicola complicating severe COVID-19. Co-infection or secondary infection of K. variicola in COVID-19 is likely under-recognized and can be fulminant as in this case.
BMC INFECTIOUS DISEASES
(2023)
Article
Immunology
Kayo Kumadaki, Natsumi Suzuki, Kaoru Tatematsu, Yohei Doi, Kentaro Tsukamoto
Summary: Bartonella species are hemotropic bacteria that cause zoonotic infections in humans. They replicate in vascular endothelial cells and induce vascular proliferation. BafA, a bacteria-secreted protein, is involved in Bartonella-induced vasoproliferation. In this study, the activity of BafA proteins derived from different Bartonella species was compared, and it was found that B. henselae, B. koehlerae, B. clarridgeiae, and B. grahamii induced cell proliferation and tube formation, while B. doshiae showed no activity. These findings suggest that BafA may contribute to the infectivity or pathogenicity of Bartonella species in humans.
INFECTION AND IMMUNITY
(2023)
Article
Infectious Diseases
Emily L. Heil, Kimberly C. Claeys, Ellen G. Kline, Tara M. Rogers, Kevin M. Squires, Alina Iovleva, Yohei Doi, Mary Banoub, Mandee M. Noval, Paul M. Luethy, Ryan K. Shields
Summary: This study evaluated the clinical characteristics and outcomes of COVID-19 patients with CRAB infections who received three-drug combination regimens. It found that the use of three-drug regimens resulted in high rates of clinical response and low mortality in severe CRAB infections among COVID-19 patients.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Medicine, General & Internal
Hiroshi Yotsuyanagi, Norio Ohmagari, Yohei Doi, Takumi Imamura, Takuhiro Sonoyama, Genki Ichihashi, Takao Sanaki, Yuko Tsuge, Takeki Uehara, Hiroshi Mukae
Summary: This study aims to evaluate the efficacy and safety of ensitrelvir in patients with mild-to-moderate COVID-19. It is a phase 3 study using a multicenter, randomized, double-blind, placebo-controlled design. The primary endpoint is the time to resolution of COVID-19 symptoms, and key secondary endpoints include the change in SARS-CoV-2 viral RNA and the time to first negative viral titer.
Article
Immunology
Natsumi Suzuki, Kayo Kumadaki, Kaoru Tatematsu, Yohei Doi, Kentaro Tsukamoto
Summary: Bartonella elizabethae is a rat-borne zoonotic bacterium that can cause human infectious endocarditis or neuroretinitis. There have been reports of bacillary angiomatosis (BA) caused by this bacterium, raising the possibility that B. elizabethae may also induce vasoproliferation. However, the effects of this bacterium on human vascular endothelial cells (ECs) are still unknown.
MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Microbiology
Mylene Vaillancourt, Anna Clara Milesi Galdino, Sam P. Limsuwannarot, Diana Celedonio, Elizabeth Dimitrova, Matthew Broerman, Catherine Bresee, Yohei Doi, Janet S. Lee, William C. Parks, Peter Jorth
Summary: This study investigates the virulence mechanisms of antibiotic resistant Pseudomonas aeruginosa in cystic fibrosis (CF) patients. It reveals that a mutation in the RNase E gene leads to increased production of siderophores, which damage macrophage cell membranes and cause cell death. The study also suggests that gallium can reduce the virulence of RNase E variants and may serve as a precision therapy for these infections.
Article
Immunology
Rick van der Geest, Hongye Fan, Hernan F. Penaloza, William G. Bain, Zeyu Xiong, Naina Kohli, Emily Larson, Mara L. G. Sullivan, Jonathan M. Franks, Donna B. Stolz, Ryota Ito, Kong Chen, Yohei Doi, Melanie J. Harriff, Janet S. Lee
Summary: This study found heterogeneity in the sensitivity of clinical Kp isolates to macrophage phagocytic uptake, and demonstrated that phagocytosis is a significant determinant of the pathogenicity of Kp in the lungs.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Michael J. Satlin, David van Duin, Pranita D. Tamma, Thomas P. Lodise, Daria Van Tyne, Keith A. Rodvold, Nadine Rouphael, Scott R. Evans, Vance G. Fowler, Toshimitsu Hamasaki, Robin Patel, Lauren Komarow, Keri Baum, Maria Souli, Nyssa Schwager, Robert A. Bonomo, Yohei Doi
Summary: Addressing antibacterial-resistant gram-negative infections is a priority of the Antibacterial Resistance Leadership Group. The focus of investigation has evolved from elucidating the epidemiology of these infections to improving patient care through clinical trials of optimizing pharmacokinetics and of novel therapies.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Microbiology
Nadine Rouphael, Patricia Winokur, Michael C. Keefer, Jessica Traenkner, Ana Drobeniuc, Yohei Doi, Sonal Munsiff, Vance G. Fowler, Scott Evans, Randolph E. Oler, Bonifride Tuyishimire, Marina Lee, Varduhi Ghazaryan, Henry F. Chambers
Summary: Concerns over resistance and safety have been identified in the current treatment regimen for complicated urinary tract infections. Fosfomycin is a drug that is routinely used for the treatment of uncomplicated cystitis. This study shows that fosfomycin could be an oral alternative as step-down therapy for the treatment of complicated urinary tract infections, with a clinical cure rate comparable to levofloxacin but a lower microbiological success rate 3 weeks from start of antibiotics.
Article
Microbiology
Richard D. Smith, Min Zhan, Shanshan Zhang, Surbhi Leekha, Anthony Harris, Yohei Doi, Scott Evans, J. Kristie Johnson, Robert K. Ernst
Summary: This study evaluated a new rapid lipid analysis technique called FLAT MS for direct identification of pathogens from blood cultures. FLAT MS showed slight differences in accuracy compared to other FDA-approved methods for identifying Gram-positive bacteria, Gram-negative bacteria, and yeast. The time-to-results and hands-on time of FLAT MS were comparable to other methods.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
